Information report for OsRab5a;gpa1
Gene Details
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Functional Descriptions
- We conclude that OsRab5a plays an essential role in trafficking of storage protein to PBII, possibly as part of its function in organizing the endomembrane system in developing endosperm cells of rice.
- OsRab5a regulates endomembrane organization and storage protein trafficking in rice endosperm cells.
- These results indicate that the formation of the paramural bodies in glup4 endosperm was due to a significant disruption of endocytosis and membrane vesicular transport by Rab5a loss of function.
- Here, we report the isolation of a rice mutant, gpa1, that accumulates 57 kDa pro-glutelins in seeds and whose endosperm has a floury appearance.
- Transmission electron microscopy analysis showed that the gpa1 endosperm cells have an enlarged ER lumen and a smaller protein body II (PBII), and accumulated three types of newly generated subcellular structures.
- Moreover, a proportion of glutelins in the gpa1 endosperm cells were not delivered to PBII, and instead were mis-targeted to two of the newly generated structures or secreted.
Literature and News
- OsRab5a regulates endomembrane organization and storage protein trafficking in rice endosperm cells . DOI: 10.1111/j.1365-313X.2010.04370.x ; PMID: 21105928
- Gene-gene interactions between mutants that accumulate abnormally high amounts of proglutelin in rice seed . DOI: 10.1270/jsbbs.60.568
- The small GTPase Rab5a is essential for intracellular transport of proglutelin from the Golgi apparatus to the protein storage vacuole and endosomal membrane organization in developing rice endosperm . DOI: 10.1104/pp.111.180505 ; PMID: 21825104
Gene Resources
- UniProt: Q0ILQ6
- EMBL: AJ292320, AK061116, AP008218
- AlphaFoldDB: Q0ILQ6
- EnsemblPlants: Os12t0631100-01, Os12t0631100-02
- Gramene: Os12t0631100-01, Os12t0631100-02
- KEGG: dosa:Os12g0631100
- Orthologous matrix: PKNEPQC
- InterPro: IPR001806, IPR005225, IPR027417
Homologs
- Actinidia chinensis: Achn273391
- Arabidopsis thaliana: AT1G06400, AT3G15060, AT1G02130, AT3G07410, AT2G44610, AT3G12160, AT3G46060, AT3G46830, AT1G05810, AT3G11730, AT1G01200, AT2G43130
- Brassica napus: GSBRNA2T00129504001
- Fragaria vesca: mrna29786.1-v1.0-hybrid
- Lupinus albus: Lalb_Chr04g0259661
- Malus domestica: MDP0000300402, MDP0000180376
- Sesamum indicum: SIN_1023555
- Solanum lycopersicum: Solyc04g012180.3
Sequences
cDNA Sequence
- >LOC_Os12g43550.2
TCTTCCTTAGCCTTAAGCGTAAAGCAACTCGTCTCATCTTCAGAGAAGCAAACCCAACAAAAAAAAAAAAAGAATTTTCAGGTTTTCTTCTCGCTTTCACCGGAGAAGGAGAAGGAGAGGAAGGTGTTGATCGAATCCTCCTCCAATCGCGCGCGATTCGATCCCCGTTGCTGGCCGCTCGCTCGCTCGATCCCTCATCCGATCTGAATCCCCCGATCTGATGGCGGCCAACCCCGGCAACAAGATCCGCAACGCCAAGCTGGTTCTTCTTGGAGATGTGGGCACGGGCAAGTCGAGCCTCGTTCTCCGGTTTGTGAAGGGCCAGTTTGTTGAGTTCCAGGAGTCCACCATCGGCGCGGCCTTCTTCTCGCAAACCTTGGCGGTTAACGATGAGACGGTGAAGTTCGAAATCTGGGATACTGCAGGGCAGGAGAGGTATCATAGCTTGGCTCCGATGTACTATCGGGGTGCGGCTGCCGCGATAGTTGTCTACGACATCACAAATGCGGCCTCTTTCACACGTGCAAAAAAATGGGTTCAAGAACTTCAAGCGCAAGGAAACCCAAACACGATAATGGCTCTTGCTGGTAACAAGGCTGATATGGTAGAGGCGAGGCAGGTGCCAGCAGAAGAGGCAAAGACCTACGCACAAGAGAATGGCCTTTTCTTCATGGAAACATCTGCGAAAACGGCGATCAATGTGAACGATGTATTCCACGAGATCGCAAAGAGATTGCTTCAAGGACAGCAGGCTCAAGACACACCGGCTGGAATGGTTCTCAACCAGAGACCAGCTGAGAGGATGGTGAGCAGTTCTTCATGCTGCTCATAATTCAAGTGTGGAGTATGTGACAACTACATAATGCCATGGCCTGAAATCCAGGAGAAGTGTGTATGCATCAGAGATGCACACAAAGCTTGTAAAGTGGCTGCTGCCATCCCAGCTGCTGTTTCTTATTTTGTCATGTTCGTTCTGTAAATGTGAATACTAGGGTGTCGCTGCAGAAATTCAGATGTATTACTACAGTGTATGCTCTTGCTTAAGTATTTCTCGACACTGAAACAGAGTAAAGCAAAGTCTAGTCAGATTCGTTTTGTTTGCTTCAATTCAGATCATGCAGTGAGCTTGCTTTTTACATTCTAATTGTGCTAGTATGTGCAATTCAGACCACATTCACCCATTTGTACCTTTTCGTCAGAAAAATGGAAAAATATGATCAGATTTTGTAGTTACCCTTTTACACAAGGAATGTGGTAAAGATACACGCATCAACTGAACAATTGCTGATGTAGCTTATCCAATTCAACATCAAGAAGTTCTGAAACCATCCCAAGCATGTGTGGCTCACAAGAAAAACAAGCATATACACGCTTAGGGAATTAACAAAAAATAAATAAAATCAGTGATGGCAACTCCTTTTGCTTATGCACCAAACCAGGAAAAAAGGGAGTACAAGAGGAAAAGCTCATGAAGGTATAGAACCATCTCACTTTTCCTGTATTGATTTTCAAGAGGGGCATGGAAACATATTATTCCAAATGAAATTCATCAGTATGTGCCATTCTTTTTTTTCTTAATGCTATCTATGTTGTTTTCACTGGATGTAATCTTCATTGTAGCTGTCACTGATCACCGAAACGAACCGCTCGAGCCATGGCAGCTGGTGAAGGTCCTTCATGGTGTCAATGATATATGAATCGGCGCAGCGGGGCGGCGACTGGCTTGGAGGCTCACCCAGGTTGAAACCCCCTGAGAGGAACCTCTGAATGAGAGCCAGCTGAGCTCTCTCCTGCTCCGAGTACCCTTCGATGGCGGTTGGCATGCAGTCGAACGTGTCCCATGCCCATATCTCGGATGCTTGGGTGGAATGCGCTGCCGAGGCAGCCTCGATGCTCTCTTCGTCCCAGAAAACACATTGGCATGTCTGTATACCATTGGTGTACTCGGCGCCACAGGAGTAGCAGAAC - >LOC_Os12g43550.1
TCTTCCTTAGCCTTAAGCGTAAAGCAACTCGTCTCATCTTCAGAGAAGCAAACCCAACAAAAAAAAAAAAAGAATTTTCAGGTTTTCTTCTCGCTTTCACCGGAGAAGGAGAAGGAGAGGAAGGTGTTGATCGAATCCTCCTCCAATCGCGCGCGATTCGATCCCCGTTGCTGGCCGCTCGCTCGCTCGATCCCTCATCCGATCTGAATCCCCCGATCTGATGGCGGCCAACCCCGGCAACAAGATCCGCAACGCCAAGCTGGTTCTTCTTGGAGATGTGGGCACGGGCAAGTCGAGCCTCGTTCTCCGGTTTGTGAAGGGCCAGTTTGTTGAGTTCCAGGAGTCCACCATCGGCGCGGCCTTCTTCTCGCAAACCTTGGCGGTTAACGATGAGACGGTGAAGTTCGAAATCTGGGATACTGCAGGGCAGGAGAGGTATCATAGCTTGGCTCCGATGTACTATCGGGGTGCGGCTGCCGCGATAGTTGTCTACGACATCACAAATGCGGCCTCTTTCACACGTGCAAAAAAATGGGTTCAAGAACTTCAAGCGCAAGGAAACCCAAACACGATAATGGCTCTTGCTGGTAACAAGGCTGATATGGTAGAGGCGAGGCAGGTGCCAGCAGAAGAGGCAAAGACCTACGCACAAGAGAATGGCCTTTTCTTCATGGAAACATCTGCGAAAACGGCGATCAATGTGAACGATGTATTCCACGAGATCGCAAAGAGATTGCTTCAAGGACAGCAGGCTCAAGACACACCGGCTGGAATGGTTCTCAACCAGAGACCAGCTGAGAGGATGGTGAGCAGTTCTTCATGCTGCTCATAATTCAAGTGTGGAGTATGTGACAACTACATAATGCCATGGCCTGAAATCCAGGAGAAGTGTGTATGCATCAGAGATGCACACAAAGCTTGTAAAGTGGCTGCTGCCATCCCAGCTGCTGTTTCTTATTTTGTCATGTTCGTTCTGTAAATGTGAATACTAGGGTGTCGCTGCAGAAATTCAGATGTATTACTACAGTGTATGCTCTTGCTTAAGTATTTCTCGACACTGAAACAGAGTAAAGCAAAGTCTAGTCAGATTCGTTTTGTTTGCTTCAATTCAGATCATGCAGTGAGCTTGCTTTTTACATTCTAATTGTGCTAGTATGTGCAATTCAGACCACATTCACCCATTTGTACCTTTTCGTCAGAAAAATGGAAAAATATGATCAGATTTTGTAGTTACCCTTTTACACAAGGAATGTGGTAAAGATACACGCATCAACTGAACAATTGCTGATGTAGCTTATCCAATTCAACATCAAGAAGTTCTGAAACCATCCCAAGCATGTGTGGCTCACAAGAAAAACAAGCATATACACGCTTAGGGAATTAACAAAAAATAAATAAAATCAGTGATGGCAACTCCTTTTGCTTATGCACCAAACCAGGAAAAAAGGGAGTACAAGAGGAAAAGCTCATGAAGGTATAGAACCATCTCACTTTTCCTGTATTGATTTTCAAGAGGGGCATGGAAACATATTATTCCAAATGAAATTCATCAGTATGTGCCATTCTTTTTTTTCTTAATGCTATCTATGTTGTTTTCACTGGATGTAATCTTCATTGTAGCTGTCACTGATCACCGAAACGAACCGCTCGAGCCATGGCAGCTGGTGAAGGTCCTTCATGGTGTCAATGATATATGAATCGGCGCAGCGGGGCGGCGACTGGCTTGGAGGCTCACCCAGGTTGAAACCCCCTGAGAGGAACCTCTGAATGAGAGCCAGCTGAGCTCTCTCCTGCTCCGAGTACCCTTCGATGGCGGTTGGCATGCAGTCGAACGTGTCCCATGCCCATATCTCGGATGCTTGGGTGGAATGCGCTGCCGAGGCAGCCTCGATGCTCTCTTCGTCCCAGAAAACACATTGGCATGTCTGTATACCATTGGTGTACTCGGCGCCACAGGAGTAGCAGAAC
CDS Sequence
- >LOC_Os12g43550.2
ATGGCGGCCAACCCCGGCAACAAGATCCGCAACGCCAAGCTGGTTCTTCTTGGAGATGTGGGCACGGGCAAGTCGAGCCTCGTTCTCCGGTTTGTGAAGGGCCAGTTTGTTGAGTTCCAGGAGTCCACCATCGGCGCGGCCTTCTTCTCGCAAACCTTGGCGGTTAACGATGAGACGGTGAAGTTCGAAATCTGGGATACTGCAGGGCAGGAGAGGTATCATAGCTTGGCTCCGATGTACTATCGGGGTGCGGCTGCCGCGATAGTTGTCTACGACATCACAAATGCGGCCTCTTTCACACGTGCAAAAAAATGGGTTCAAGAACTTCAAGCGCAAGGAAACCCAAACACGATAATGGCTCTTGCTGGTAACAAGGCTGATATGGTAGAGGCGAGGCAGGTGCCAGCAGAAGAGGCAAAGACCTACGCACAAGAGAATGGCCTTTTCTTCATGGAAACATCTGCGAAAACGGCGATCAATGTGAACGATGTATTCCACGAGATCGCAAAGAGATTGCTTCAAGGACAGCAGGCTCAAGACACACCGGCTGGAATGGTTCTCAACCAGAGACCAGCTGAGAGGATGGTGAGCAGTTCTTCATGCTGCTCATAA - >LOC_Os12g43550.1
ATGGCGGCCAACCCCGGCAACAAGATCCGCAACGCCAAGCTGGTTCTTCTTGGAGATGTGGGCACGGGCAAGTCGAGCCTCGTTCTCCGGTTTGTGAAGGGCCAGTTTGTTGAGTTCCAGGAGTCCACCATCGGCGCGGCCTTCTTCTCGCAAACCTTGGCGGTTAACGATGAGACGGTGAAGTTCGAAATCTGGGATACTGCAGGGCAGGAGAGGTATCATAGCTTGGCTCCGATGTACTATCGGGGTGCGGCTGCCGCGATAGTTGTCTACGACATCACAAATGCGGCCTCTTTCACACGTGCAAAAAAATGGGTTCAAGAACTTCAAGCGCAAGGAAACCCAAACACGATAATGGCTCTTGCTGGTAACAAGGCTGATATGGTAGAGGCGAGGCAGGTGCCAGCAGAAGAGGCAAAGACCTACGCACAAGAGAATGGCCTTTTCTTCATGGAAACATCTGCGAAAACGGCGATCAATGTGAACGATGTATTCCACGAGATCGCAAAGAGATTGCTTCAAGGACAGCAGGCTCAAGACACACCGGCTGGAATGGTTCTCAACCAGAGACCAGCTGAGAGGATGGTGAGCAGTTCTTCATGCTGCTCATAA
Protein Sequence
- >LOC_Os12g43550.2
MAANPGNKIRNAKLVLLGDVGTGKSSLVLRFVKGQFVEFQESTIGAAFFSQTLAVNDETVKFEIWDTAGQERYHSLAPMYYRGAAAAIVVYDITNAASFTRAKKWVQELQAQGNPNTIMALAGNKADMVEARQVPAEEAKTYAQENGLFFMETSAKTAINVNDVFHEIAKRLLQGQQAQDTPAGMVLNQRPAERMVSSSSCCS* - >LOC_Os12g43550.1
MAANPGNKIRNAKLVLLGDVGTGKSSLVLRFVKGQFVEFQESTIGAAFFSQTLAVNDETVKFEIWDTAGQERYHSLAPMYYRGAAAAIVVYDITNAASFTRAKKWVQELQAQGNPNTIMALAGNKADMVEARQVPAEEAKTYAQENGLFFMETSAKTAINVNDVFHEIAKRLLQGQQAQDTPAGMVLNQRPAERMVSSSSCCS*