Gene Details:

Functional Descriptions:

  • Seedlings of transgenic Arabidopsis thaliana plants overexpressing OsRAN2 were overly sensitive to salinity stress and exogenous ABA treatment.
  • Here, we investigated OsRAN2 involved in regulation of cell division during cold stress in rice.
  • OsRAN2, essential for mitosis, enhances cold tolerance in rice by promoting export of intranuclear tubulin and maintaining cell division under cold stress.
  • Our study suggests a mechanism for OsRAN2 in regulating cold resistance in rice by maintaining cell division through promoting the normal export of intranuclear tubulin at the end of mitosis.
  • Expression of OsRAN2 was increased under cold treatment, but not during salt and drought stress.
  • OsRAN2 overexpression in tobacco epidermal leaf cells disturbed the nuclear import of a maize (Zea mays L.
  • Overexpression of OsRAN2 in rice resulted in enhanced sensitivity to salinity, osmotic stress, and ABA.
  • Overexpression of OsRAN2 in rice and Arabidopsis renders transgenic plants hypersensitive to salinity and osmotic stress.
  • The mean root mitotic index was closely related to the expression level of OsRAN2.
  • The transgenic rice overexpressing OsRAN2 showed maintained cell division, decreased proportion of cells with intranuclear tubulin and formation of a normal nuclear envelope under the cold condition.
  • Transcription of OsRAN2 was reduced by salt, osmotic, and exogenous abscisic acid (ABA) treatments, as determined by real-time PCR.
  • Overexpression of OsRAN2 enhanced cold tolerance in rice.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os05g49890.3
    CAATTCCATTTCGAATTCTAGGGTTTTGTTGGCCCCCATTCCTCCCCCACCCCGGTGTCCTCCCCTCCGCCGCTCGCGTCGCCGCCTTTTTCCCCTATGGCGCTGCCGAATCAGGGGACCGTGGATTACCCCAGCTTCAAGCTGGTCATCGTCGGCGATGGCGGGACTGGTAAAACTACATTTGTGAAGAGGCATCTCACTGGAGAGTTTGAAAAGAAATATGAGCCAACCATTGGTGTCGAAGTTCACCCTCTAGATTTTACCACCAACTGTGGTAAGATCCGCTTCTACTGCTGGGACACTGCTGGACAAGAGAAGTTTGGTGGACTTAGGGATGGATACTATATCCATGGTCAATGTGCGATAATTATGTTTGATGTCACTTCAAGGCTGACTTACAAGAATGTTCCAACATGGCATAGGGACTTGTGCAGGTTTATTTCTGAAGACACGATAAACTTTACCTAATATATTATATGGAAGAGAACTCTTATGATTAGGTATCAACTCTTGCAGGGTGTGTGAAAACATCCCCATTGTCCTGTGTGGTAACAAGGTTGATGTGAAGAACAGGCAGGTTAAAGCCAAGCAGGTCACATTCCACAGGAAGAAGAATCTCCAGTACTATGAAATTTCTGCCAAGAGCAACTACAACTTTGAGAAGCCCTTCCTTTATCTTGCAAGGAAGCTTGCTGGTGACCCGAACCTGCATTTCGTTGAAGCTGTTGCTCTTAAACCTCCGGAAGTTCCCATTGACCTGGCAATGCAGCAACAGCATGAGGCTGAGCTTGCGGCTGCAGCAGCACAACCTCTCCCAGATGACGATGACGATCTGATCGAGTAGAGAGGATCATGAGTCTATCATGCTTCAGCCTGAGTCTCTTCATAAGAGTCTGAATTAGGCCAATGCATGCTAGTGTTAGCTTGTCTGTGGAATGTGGACGTTTCCTGCGTGCCTGATGGGTTGAAAACTTTTGATGTGTGGTGTTACATGTTGCTGCCATGAATTTGTTGGCTCTTTTTTAACACTGCTGTGTTACTTCTATGGTTATGCTTAAACTATTTGTATCATCGAGCTGGTATTTATTTATCCGGTGTTGCACAATATTCTTCTTCACTGCCCTAACTGCGAGTGCTGACCGCCCAAGATTATGTAATGTTCGCATCGCATTAAGTTTTGTATATCTGTGATGAAA
  • >LOC_Os05g49890.1
    CAATTCCATTTCGAATTCTAGGGTTTTGTTGGCCCCCATTCCTCCCCCACCCCGGTGTCCTCCCCTCCGCCGCTCGCGTCGCCGCCTTTTTCCCCTATGGCGCTGCCGAATCAGGGGACCGTGGATTACCCCAGCTTCAAGCTGGTCATCGTCGGCGATGGCGGGACTGGTAAAACTACATTTGTGAAGAGGCATCTCACTGGAGAGTTTGAAAAGAAATATGAGCCAACCATTGGTGTCGAAGTTCACCCTCTAGATTTTACCACCAACTGTGGTAAGATCCGCTTCTACTGCTGGGACACTGCTGGACAAGAGAAGTTTGGTGGACTTAGGGATGGATACTATATCCATGGTCAATGTGCGATAATTATGTTTGATGTCACTTCAAGGCTGACTTACAAGAATGTTCCAACATGGCATAGGGACTTGTGCAGGTTTATTTCTGAAGACACGATAAACTTTACCTAATATATTATATGGAAGAGAACTCTTATGATTAGGTATCAACTCTTGCAGGGTGTGTGAAAACATCCCCATTGTCCTGTGTGGTAACAAGGTTGATGTGAAGAACAGGCAGGTTAAAGCCAAGCAGGTCACATTCCACAGGAAGAAGAATCTCCAGTACTATGAAATTTCTGCCAAGAGCAACTACAACTTTGAGAAGCCCTTCCTTTATCTTGCAAGGAAGCTTGCTGGTGACCCGAACCTGCATTTCGTTGAAGCTGTTGCTCTTAAACCTCCGGAAGTTCCCATTGACCTGGCAATGCAGCAACAGCATGAGGCTGAGCTTGCGGCTGCAGCAGCACAACCTCTCCCAGATGACGATGACGATCTGATCGAGTAGAGAGGATCATGAGTCTATCATGCTTCAGCCTGAGTCTCTTCATAAGAGTCTGAATTAGGCCAATGCATGCTAGTGTTAGCTTGTCTGTGGAATGTGGACGTTTCCTGCGTGCCTGATGGGTTGAAAACTTTTGATGTGTGGTGTTACATGTTGCTGCCATGAATTTGTTGGCTCTTTTTTAACACTGCTGTGTTACTTCTATGGTTATGCTTAAACTATTTGTATCATCGAGCTGGTATTTATTTATCCGGTGTTGCACAATATTCTTCTTCACTGCCCTAACTGCGAGTGCTGACCGCCCAAGATTATGTAATGTTCGCATCGCATTAAGTTTTGTATATCTGTGATGAAA
  • >LOC_Os05g49890.2
    CAATTCCATTTCGAATTCTAGGGTTTTGTTGGCCCCCATTCCTCCCCCACCCCGGTGTCCTCCCCTCCGCCGCTCGCGTCGCCGCCTTTTTCCCCTATGGCGCTGCCGAATCAGGGGACCGTGGATTACCCCAGCTTCAAGCTGGTCATCGTCGGCGATGGCGGGACTGGTAAAACTACATTTGTGAAGAGGCATCTCACTGGAGAGTTTGAAAAGAAATATGAGCCAACCATTGGTGTCGAAGTTCACCCTCTAGATTTTACCACCAACTGTGGTAAGATCCGCTTCTACTGCTGGGACACTGCTGGACAAGAGAAGTTTGGTGGACTTAGGGATGGATACTATATCCATGGTCAATGTGCGATAATTATGTTTGATGTCACTTCAAGGCTGACTTACAAGAATGTTCCAACATGGCATAGGGACTTGTGCAGGTTTATTTCTGAAGACACGATAAACTTTACCTAATATATTATATGGAAGAGAACTCTTATGATTAGGTATCAACTCTTGCAGGGTGTGTGAAAACATCCCCATTGTCCTGTGTGGTAACAAGGTTGATGTGAAGAACAGGCAGGTTAAAGCCAAGCAGGTCACATTCCACAGGAAGAAGAATCTCCAGTACTATGAAATTTCTGCCAAGAGCAACTACAACTTTGAGAAGCCCTTCCTTTATCTTGCAAGGAAGCTTGCTGGTGACCCGAACCTGCATTTCGTTGAAGCTGTTGCTCTTAAACCTCCGGAAGTTCCCATTGACCTGGCAATGCAGCAACAGCATGAGGCTGAGCTTGCGGCTGCAGCAGCACAACCTCTCCCAGATGACGATGACGATCTGATCGAGTAGAGAGGATCATGAGTCTATCATGCTTCAGCCTGAGTCTCTTCATAAGAGTCTGAATTAGGCCAATGCATGCTAGTGTTAGCTTGTCTGTGGAATGTGGACGTTTCCTGCGTGCCTGATGGGTTGAAAACTTTTGATGTGTGGTGTTACATGTTGCTGCCATGAATTTGTTGGCTCTTTTTTAACACTGCTGTGTTACTTCTATGGTTATGCTTAAACTATTTGTATCATCGAGCTGGTATTTATTTATCCGGTGTTGCACAATATTCTTCTTCACTGCCCTAACTGCGAGTGCTGACCGCCCAAGATTATGTAATGTTCGCATCGCATTAAGTTTTGTATATCTGTGATGAAA
CDS Sequence
  • >LOC_Os05g49890.3
    ATGGCGCTGCCGAATCAGGGGACCGTGGATTACCCCAGCTTCAAGCTGGTCATCGTCGGCGATGGCGGGACTGGTAAAACTACATTTGTGAAGAGGCATCTCACTGGAGAGTTTGAAAAGAAATATGAGCCAACCATTGGTGTCGAAGTTCACCCTCTAGATTTTACCACCAACTGTGGTAAGATCCGCTTCTACTGCTGGGACACTGCTGGACAAGAGAAGTTTGGTGGACTTAGGGATGGATACTATATCCATGGTCAATGTGCGATAATTATGTTTGATGTCACTTCAAGGCTGACTTACAAGAATGTTCCAACATGGCATAGGGACTTGTGCAGGTTTATTTCTGAAGACACGATAAACTTTACCTAA
  • >LOC_Os05g49890.1
    ATGGCGCTGCCGAATCAGGGGACCGTGGATTACCCCAGCTTCAAGCTGGTCATCGTCGGCGATGGCGGGACTGGTAAAACTACATTTGTGAAGAGGCATCTCACTGGAGAGTTTGAAAAGAAATATGAGCCAACCATTGGTGTCGAAGTTCACCCTCTAGATTTTACCACCAACTGTGGTAAGATCCGCTTCTACTGCTGGGACACTGCTGGACAAGAGAAGTTTGGTGGACTTAGGGATGGATACTATATCCATGGTCAATGTGCGATAATTATGTTTGATGTCACTTCAAGGCTGACTTACAAGAATGTTCCAACATGGCATAGGGACTTGTGCAGGTTTATTTCTGAAGACACGATAAACTTTACCTAA
  • >LOC_Os05g49890.2
    ATGGCGCTGCCGAATCAGGGGACCGTGGATTACCCCAGCTTCAAGCTGGTCATCGTCGGCGATGGCGGGACTGGTAAAACTACATTTGTGAAGAGGCATCTCACTGGAGAGTTTGAAAAGAAATATGAGCCAACCATTGGTGTCGAAGTTCACCCTCTAGATTTTACCACCAACTGTGGTAAGATCCGCTTCTACTGCTGGGACACTGCTGGACAAGAGAAGTTTGGTGGACTTAGGGATGGATACTATATCCATGGTCAATGTGCGATAATTATGTTTGATGTCACTTCAAGGCTGACTTACAAGAATGTTCCAACATGGCATAGGGACTTGTGCAGGTTTATTTCTGAAGACACGATAAACTTTACCTAA
Protein Sequence
  • >LOC_Os05g49890.3
    MALPNQGTVDYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFTTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTSRLTYKNVPTWHRDLCRFISEDTINFT*
  • >LOC_Os05g49890.1
    MALPNQGTVDYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFTTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTSRLTYKNVPTWHRDLCRFISEDTINFT*
  • >LOC_Os05g49890.2
    MALPNQGTVDYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFTTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTSRLTYKNVPTWHRDLCRFISEDTINFT*