Gene Details:

Functional Descriptions:

  • The 4-bp (CGAG) insertion may cause a weak or loss-of-function allele of ZmPLA1, leading to the HI phenotype.
  • ZmPLA1 (GRMZM2G471240) Mutation Causes Haploid Induction in Maize.
  • A 4-bp Insertion at ZmPLA1 Encoding a Putative Phospholipase A Generates Haploid Induction in Maize.
  • ZmPLA1/MTL-mediated HI may facilitate to enhance breeding efficiency not only in maize but also in other important crops.
  • ZmPLA1 encodes a pollen-specific phospholipase; novel edits lead to a 6.7% haploid induction rate.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Zm00001eb019170_T002
    GTTCATCACTAATCACACTTATTGTGCCCTCGACGAGTATCTATAGCTAGCTCATTAATCGATTCGGGGGTGTGTTGTCGAAGGCGGCAATGGCGAGCTACTCGTCGCGGCGTCCATGCAATACCTGTAGCACGAAGGCGATGGCCGGGAGCGTGGTCGGCGAGCCCGTCGTGCTGGGGCAGAGGGTGACGGTGCTGACGGTGGACGGCGGCGGCGTCCGGGGTCTCATCCCGGGAACCATCCTCGCCTTCCTGGAGGCCAGGCTGCAGGAGCTGGACGGACCGGAGGCGAGGCTGGCGGACTACTTCGACTACATCGCCGGAACCAGCACCGGCGGTCTCATCACCGCCATGCTCACCGCGCCCGGCAAGGACAAGCGGCCTCTCTACGCTGCCAAGGACATCAACCACTTTTACATGCAGAACTGCCCGCGCATCTTTCCTCAGAAGAGCAGGCTTGCGGCCGCCATGTCCGCGCTGAGGAAGCCAAAGTACAACGGCAAGTGCATGCGCAGCCTGATTAGGAGCATCCTCGGCGAGACGAGGGTAAGCGAGACGCTGACCAACGTCATCATCCCTGCCTTCGACATCAGGCTGCTGCAGCCTATCATCTTCTCTACCTACGACGCCAAGAGCACGCCTCTGAAGAACGCTCTGCTCTCGGACGTGTGCATTGGCACGTCCGCCGCGCCGACCTACCTCCCGGCGCACTACTTCCAGACTGAAGACGCCAACGGCAAGGAGCGCGAATACAACCTCATCGACGGCGGTGTGGCGGCCAACAACCCGACGATGGTTGCGATGACGCAGATCACCAAAAAGATGCTTGCCAGCAAGGACAAGGCCGAGGAGCTGTACCCAGTGAAGCCGTCGAACTGCCGCAGGTTCCTGGTGCTGTCCATCGGGACGGGGTCGACGTCCGAGCAGGGCCTCTACACGGCGCGGCAGTGCTCCCGGTGGGGTATCTGCCGGTGGCTCCGCAACAACGGCATGGCCCCCATCATCGACATCTTCATGGCGGCCAGCTCGGACCTGGTGGACATCCACGTCGCCGCGATGTTCCAGTCGCTCCACAGCGACGGCGACTACCTGCGCATCCAGGACAACTCGCTCCGTGGCGCCGCGGCCACCGTGGACGCGGCGACGCCGGAGAACATGCGGACGCTCGTCGGGATCGGGGAGCGGATGCTGGCACAGAGGGTGTCCAGGGTCAACGTGGAGACAGGGAGGTACGAACCGGTGACTGGCGAAGGAAGCAATGCCGATGCCCTCGGTGGGCTCGCTAGGCAGCTCTCCGAGGAGAGGAGAACAAGGCTCGCGCGCCGCGTCTCTGCCATCAACCCAAGAGGCTCTAGATGTGCGTCGTACGATATCTAAGACAAGTGGCTTTACTGTCAGTCACATGCTTGTAAATAAGTAGACTTTATTTTAATAAAACATAAAAATATATATATGTTCTTGAATATAAAATTGATAACCAAATTAAAATTCGAACCATCACTTATACATAATTTTACTTTATTTTTTATAAAAC
  • >Zm00001eb019170_T001
    GTTCATCACTAATCACACTTATTGTGCCCTCGACGAGTATCTATAGCTAGCTCATTAATCGATTCGGGGGTGTGTTGTCGAAGGCGGCAATGGCGAGCTACTCGTCGCGGCGTCCATGCAATACCTGTAGCACGAAGGCGATGGCCGGGAGCGTGGTCGGCGAGCCCGTCGTGCTGGGGCAGAGGGTGACGGTGCTGACGGTGGACGGCGGCGGCGTCCGGGGTCTCATCCCGGGAACCATCCTCGCCTTCCTGGAGGCCAGGCTGCAGGAGCTGGACGGACCGGAGGCGAGGCTGGCGGACTACTTCGACTACATCGCCGGAACCAGCACCGGCGGTCTCATCACCGCCATGCTCACCGCGCCCGGCAAGGACAAGCGGCCTCTCTACGCTGCCAAGGACATCAACCACTTTTACATGCAGAACTGCCCGCGCATCTTTCCTCAGAAGAGCAGGCTTGCGGCCGCCATGTCCGCGCTGAGGAAGCCAAAGTACAACGGCAAGTGCATGCGCAGCCTGATTAGGAGCATCCTCGGCGAGACGAGGGTAAGCGAGACGCTGACCAACGTCATCATCCCTGCCTTCGACATCAGGCTGCTGCAGCCTATCATCTTCTCTACCTACGACGCCAAGAGCACGCCTCTGAAGAACGCTCTGCTCTCGGACGTGTGCATTGGCACGTCCGCCGCGCCGACCTACCTCCCGGCGCACTACTTCCAGACTGAAGACGCCAACGGCAAGGAGCGCGAATACAACCTCATCGACGGCGGTGTGGCGGCCAACAACCCGACGATGGTTGCGATGACGCAGATCACCAAAAAGATGCTTGCCAGCAAGGACAAGGCCGAGGAGCTGTACCCAGTGAAGCCGTCGAACTGCCGCAGGTTCCTGGTGCTGTCCATCGGGACGGGGTCGACGTCCGAGCAGGGCCTCTACACGGCGCGGCAGTGCTCCCGGTGGGGTATCTGCCGGTGGCTCCGCAACAACGGCATGGCCCCCATCATCGACATCTTCATGGCGGCCAGCTCGGACCTGGTGGACATCCACGTCGCCGCGATGTTCCAGTCGCTCCACAGCGACGGCGACTACCTGCGCATCCAGGACAACTCGCTCCGTGGCGCCGCGGCCACCGTGGACGCGGCGACGCCGGAGAACATGCGGACGCTCGTCGGGATCGGGGAGCGGATGCTGGCACAGAGGGTGTCCAGGGTCAACGTGGAGACAGGGAGGTACGAACCGGTGACTGGCGAAGGAAGCAATGCCGATGCCCTCGGTGGGCTCGCTAGGCAGCTCTCCGAGGAGAGGAGAACAAGGCTCGCGCGCCGCGTCTCTGCCATCAACCCAAGAGGCTCTAGATGTGCGTCGTACGATATCTAAGACAAGTGGCTTTACTGTCAGTCACATGCTTGTAAATAAGTAGACTTTATTTTAATAAAACATAAAAATATATATATGTTCTTGAATATAAAATTGATAACCAAATTAAAATTCGAACCATCACTTATACATAATTTTACTTTATTTTTTATAAAAC
CDS Sequence
  • >Zm00001eb019170_T002
    ATGGCGAGCTACTCGTCGCGGCGTCCATGCAATACCTGTAGCACGAAGGCGATGGCCGGGAGCGTGGTCGGCGAGCCCGTCGTGCTGGGGCAGAGGGTGACGGTGCTGACGGTGGACGGCGGCGGCGTCCGGGGTCTCATCCCGGGAACCATCCTCGCCTTCCTGGAGGCCAGGCTGCAGGAGCTGGACGGACCGGAGGCGAGGCTGGCGGACTACTTCGACTACATCGCCGGAACCAGCACCGGCGGTCTCATCACCGCCATGCTCACCGCGCCCGGCAAGGACAAGCGGCCTCTCTACGCTGCCAAGGACATCAACCACTTTTACATGCAGAACTGCCCGCGCATCTTTCCTCAGAAGAGCAGGCTTGCGGCCGCCATGTCCGCGCTGAGGAAGCCAAAGTACAACGGCAAGTGCATGCGCAGCCTGATTAGGAGCATCCTCGGCGAGACGAGGGTAAGCGAGACGCTGACCAACGTCATCATCCCTGCCTTCGACATCAGGCTGCTGCAGCCTATCATCTTCTCTACCTACGACGCCAAGAGCACGCCTCTGAAGAACGCTCTGCTCTCGGACGTGTGCATTGGCACGTCCGCCGCGCCGACCTACCTCCCGGCGCACTACTTCCAGACTGAAGACGCCAACGGCAAGGAGCGCGAATACAACCTCATCGACGGCGGTGTGGCGGCCAACAACCCGACGATGGTTGCGATGACGCAGATCACCAAAAAGATGCTTGCCAGCAAGGACAAGGCCGAGGAGCTGTACCCAGTGAAGCCGTCGAACTGCCGCAGGTTCCTGGTGCTGTCCATCGGGACGGGGTCGACGTCCGAGCAGGGCCTCTACACGGCGCGGCAGTGCTCCCGGTGGGGTATCTGCCGGTGGCTCCGCAACAACGGCATGGCCCCCATCATCGACATCTTCATGGCGGCCAGCTCGGACCTGGTGGACATCCACGTCGCCGCGATGTTCCAGTCGCTCCACAGCGACGGCGACTACCTGCGCATCCAGGACAACTCGCTCCGTGGCGCCGCGGCCACCGTGGACGCGGCGACGCCGGAGAACATGCGGACGCTCGTCGGGATCGGGGAGCGGATGCTGGCACAGAGGGTGTCCAGGGTCAACGTGGAGACAGGGAGGTACGAACCGGTGACTGGCGAAGGAAGCAATGCCGATGCCCTCGGTGGGCTCGCTAGGCAGCTCTCCGAGGAGAGGAGAACAAGGCTCGCGCGCCGCGTCTCTGCCATCAACCCAAGAGGCTCTAGATGTGCGTCGTACGATATCTAA
  • >Zm00001eb019170_T001
    ATGGCGAGCTACTCGTCGCGGCGTCCATGCAATACCTGTAGCACGAAGGCGATGGCCGGGAGCGTGGTCGGCGAGCCCGTCGTGCTGGGGCAGAGGGTGACGGTGCTGACGGTGGACGGCGGCGGCGTCCGGGGTCTCATCCCGGGAACCATCCTCGCCTTCCTGGAGGCCAGGCTGCAGGAGCTGGACGGACCGGAGGCGAGGCTGGCGGACTACTTCGACTACATCGCCGGAACCAGCACCGGCGGTCTCATCACCGCCATGCTCACCGCGCCCGGCAAGGACAAGCGGCCTCTCTACGCTGCCAAGGACATCAACCACTTTTACATGCAGAACTGCCCGCGCATCTTTCCTCAGAAGAGCAGGCTTGCGGCCGCCATGTCCGCGCTGAGGAAGCCAAAGTACAACGGCAAGTGCATGCGCAGCCTGATTAGGAGCATCCTCGGCGAGACGAGGGTAAGCGAGACGCTGACCAACGTCATCATCCCTGCCTTCGACATCAGGCTGCTGCAGCCTATCATCTTCTCTACCTACGACGCCAAGAGCACGCCTCTGAAGAACGCTCTGCTCTCGGACGTGTGCATTGGCACGTCCGCCGCGCCGACCTACCTCCCGGCGCACTACTTCCAGACTGAAGACGCCAACGGCAAGGAGCGCGAATACAACCTCATCGACGGCGGTGTGGCGGCCAACAACCCGACGATGGTTGCGATGACGCAGATCACCAAAAAGATGCTTGCCAGCAAGGACAAGGCCGAGGAGCTGTACCCAGTGAAGCCGTCGAACTGCCGCAGGTTCCTGGTGCTGTCCATCGGGACGGGGTCGACGTCCGAGCAGGGCCTCTACACGGCGCGGCAGTGCTCCCGGTGGGGTATCTGCCGGTGGCTCCGCAACAACGGCATGGCCCCCATCATCGACATCTTCATGGCGGCCAGCTCGGACCTGGTGGACATCCACGTCGCCGCGATGTTCCAGTCGCTCCACAGCGACGGCGACTACCTGCGCATCCAGGACAACTCGCTCCGTGGCGCCGCGGCCACCGTGGACGCGGCGACGCCGGAGAACATGCGGACGCTCGTCGGGATCGGGGAGCGGATGCTGGCACAGAGGGTGTCCAGGGTCAACGTGGAGACAGGGAGGTACGAACCGGTGACTGGCGAAGGAAGCAATGCCGATGCCCTCGGTGGGCTCGCTAGGCAGCTCTCCGAGGAGAGGAGAACAAGGCTCGCGCGCCGCGTCTCTGCCATCAACCCAAGAGGCTCTAGATGTGCGTCGTACGATATCTAA
Protein Sequence
  • >Zm00001eb019170_P002
    MASYSSRRPCNTCSTKAMAGSVVGEPVVLGQRVTVLTVDGGGVRGLIPGTILAFLEARLQELDGPEARLADYFDYIAGTSTGGLITAMLTAPGKDKRPLYAAKDINHFYMQNCPRIFPQKSRLAAAMSALRKPKYNGKCMRSLIRSILGETRVSETLTNVIIPAFDIRLLQPIIFSTYDAKSTPLKNALLSDVCIGTSAAPTYLPAHYFQTEDANGKEREYNLIDGGVAANNPTMVAMTQITKKMLASKDKAEELYPVKPSNCRRFLVLSIGTGSTSEQGLYTARQCSRWGICRWLRNNGMAPIIDIFMAASSDLVDIHVAAMFQSLHSDGDYLRIQDNSLRGAAATVDAATPENMRTLVGIGERMLAQRVSRVNVETGRYEPVTGEGSNADALGGLARQLSEERRTRLARRVSAINPRGSRCASYDI
  • >Zm00001eb019170_P001
    MASYSSRRPCNTCSTKAMAGSVVGEPVVLGQRVTVLTVDGGGVRGLIPGTILAFLEARLQELDGPEARLADYFDYIAGTSTGGLITAMLTAPGKDKRPLYAAKDINHFYMQNCPRIFPQKSRLAAAMSALRKPKYNGKCMRSLIRSILGETRVSETLTNVIIPAFDIRLLQPIIFSTYDAKSTPLKNALLSDVCIGTSAAPTYLPAHYFQTEDANGKEREYNLIDGGVAANNPTMVAMTQITKKMLASKDKAEELYPVKPSNCRRFLVLSIGTGSTSEQGLYTARQCSRWGICRWLRNNGMAPIIDIFMAASSDLVDIHVAAMFQSLHSDGDYLRIQDNSLRGAAATVDAATPENMRTLVGIGERMLAQRVSRVNVETGRYEPVTGEGSNADALGGLARQLSEERRTRLARRVSAINPRGSRCASYDI

Source: Genetic information comes from the article