Gene Details:

• Gene ID: Zm00001eb317040
• Gene Symbol: ZmTKPR1-2
• Gene Name: dihydroflavonoid reductase1
• Genome: Zm-B73-REFERENCE-NAM-5.0
• Species: Zea mays

Functional Descriptions:

• CRISPR/Cas9-based genome editing of 14 lipid metabolic genes reveals a sporopollenin metabolon ZmPKSB-ZmTKPR1-1/-2 required for pollen exine formation in maize.
• Identified two maize GMS genes, ZmTKPR1-1 and ZmTKPR1-2, by CRISPR/Cas9 mutagenesis of 14 lipid metabolic genes with anther stage-specific expression patterns.
• ZmTKPR1-1 and ZmTKPR1-2 encode tetraketide α-pyrone reductases and have catalytic activities in reducing tetraketide α-pyrone produced by ZmPKSB (polyketide synthase B).
• ZmTKPR1-1/ZmTKPR1-2 and ZmPKSB formed a protein complex, their mutants showed different, even opposite, defective phenotypes of anther cuticle and pollen exine.
• Based on protein structure prediction, molecular docking, site-directed mutagenesis and biochemical assays, the sporopollenin biosynthetic metabolon ZmPKSB-ZmTKPR1-1/ZmTKPR1-2 was identified to control pollen exine formation in maize anther.

Function-related keywords:

• pollen ,  anther ,  cuticle ,  pollen-exine-formation ,  pollen-formation ,  cuticle-formation ,  protein

Literature:

• CRISPR/Cas9-based genome editing of 14 lipid metabolic genes reveals a sporopollenin metabolon ZmPKSB-ZmTKPR1-1/-2 required for pollen exine formation in maize.   DOI:  10.1111/pbi.14181 ;  PMID:  37792967

Related News:

Gene Resources:

• NCBI ID: LOC542650
• UniProt accessions: A0A804QC34

Orthologs:

Sequences:

Source: Genetic information comes from the article