Information report for TaMTL-4A
Gene Details
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Functional Descriptions
- TaWaxy and TaMTL, were edited with high efficiency by the optimized SpCas9 system, with the highest efficiency (80.5%) being achieved when using TaU3 and two sgRNAs to target TaWaxy.
- A haploid induction rate of 18.9% was found in the TaMTL-edited T1 plants using the CRISPR/SpCas9 system. Mutants with reverse insertion of the deleted sequences of TaMTL and TaWaxy between the two sgRNAs were identified in the edited T0 plants.
- In addition, wheat grains lacking embryos or endosperms were observed in the TaMTL-edited T1 generation.
- We then applied the optimized system to edit TaMTL and TaWaxy to develop the induction of haploids and to improve starch quality.
Literature and News
Gene Resources
- NCBI ID: KAF7041134
- UniProt accessions: A0A3B6HPB9
- EnsemblPlants: TraesCS4A02G018100.1
- Gramene: TraesCS4A02G018100.1
- GO: GO:0004620 , GO:0006952 , GO:0016042 , GO:0047372
- OMA: RAMAGCV
- InterPro: IPR002641 , IPR016035
- PANTHER: PTHR32176 , PTHR32176:SF5
- SUPFAM: SSF52151
- PROSITE: PS51635
- Gene3D: 3.40.1090.10
- OrthoDB: A0A3B6HPB9
- SMR: A0A3B6HPB9
Sequences
cDNA Sequence
- >TraesCS4A02G018100.1
ATGGCAAGCTACTGGTGCCGGCGGCCCTGCGAGTCGTGCAGCACGCGGGCGATGGCGGGCAGCGTGGTCGGCCAGCCGGTGGCGCCGGGGCAGCGGGTGACGGTGCTGACCATCGACGGGGGCGGCATCCGCGGCATCATCCCGGGCACCATCCTCGACTTCCTCGAGGCCAAGCTGCAGGAGCTGGACGGCCCGGGCGCGCGCCTGGCCGACTACTTCGACTGCATCGCCGGCACCAGCACCGGCGGCCTCATCACCGCCATGATCACCGCGCCCGGCAAGGACGGCCGCCCGCTCTTCGCCGCCAGGGACGTCAACCGCTTCTACCTCGACAACGGGCCCTACATCTTCCCTCAAAGGAGGTGCGCGCTCGCCGCGGTGACCGCATCGCTGAGGCGGCCGAGGTACAGCGGCAAGTACCTGCACGGCAAGATCAGGAGCATGCTCGGCGAGACGAGGCTGTGCGACGCGCTCACCGACGTCGTCATCCCCACCTTCGACGTCAAGCTTCTCCAGCCCATCATCTTCTCCACATACGACGCCAAGAGCATGCCCCTGAAGAACGCGCGGCTCGCCGACATCTGCATCGGCACCTCCGCCGCCCCGACCTACCTCCCCGCGCACCACTTCCACACCCACGACGGCAACGGCAAGGAGCGCGAGTACAACCTCATCGACGGCGGCGTCGCCGCCAACAATCCGACGATGGTGGCGATGACGCAGATCACCAAGAAGATGATGGGCAAGGACAGGGAGGAGCTGTACCCGGTGAAGCCGTCGGACTGCGGCAAGTTCCTGGTGATGTCCGTCGGCACCGGCTCGACGTCCGACCAGGGGCTGTACACGGCGAAGCAGTGCTCCCAGTGGGGCATCATCAGCTGGCTCCGCAACAAGGGCATGGCGCCCATCATCGACATCTTCATGGCAGCCAGCTCCGACCTCGTCGACATCCACGCCGCCGTGCTCTTCCAGTCGCTGCACAGCGACGGCGACTACCTCCGCATCCAGGACAACTCGCTCCACGGCCCGGCGGCCACGGTGGACGCTGCCACGCCCGAGAACATGGCGGAGCTCCTCAGGATCGGCGAGCGGATGCTGGCGCAGAGGGTGTCCAGGGTGAACGTCGAGACCGGGAGGTACGAGGAGGTAAAGGGGGCCGGGAGCAACGCCGACGCGCTCGCCGGGTTCGCCAGGCAGCTCTCCGACGAGAGGAGGACAAGGCTCGAGCGCCGGCGTGGTGGCGCCGGCCGTCTGAAATCCAGACGCTGA
CDS Sequence
- >TraesCS4A02G018100.1
ATGGCAAGCTACTGGTGCCGGCGGCCCTGCGAGTCGTGCAGCACGCGGGCGATGGCGGGCAGCGTGGTCGGCCAGCCGGTGGCGCCGGGGCAGCGGGTGACGGTGCTGACCATCGACGGGGGCGGCATCCGCGGCATCATCCCGGGCACCATCCTCGACTTCCTCGAGGCCAAGCTGCAGGAGCTGGACGGCCCGGGCGCGCGCCTGGCCGACTACTTCGACTGCATCGCCGGCACCAGCACCGGCGGCCTCATCACCGCCATGATCACCGCGCCCGGCAAGGACGGCCGCCCGCTCTTCGCCGCCAGGGACGTCAACCGCTTCTACCTCGACAACGGGCCCTACATCTTCCCTCAAAGGAGGTGCGCGCTCGCCGCGGTGACCGCATCGCTGAGGCGGCCGAGGTACAGCGGCAAGTACCTGCACGGCAAGATCAGGAGCATGCTCGGCGAGACGAGGCTGTGCGACGCGCTCACCGACGTCGTCATCCCCACCTTCGACGTCAAGCTTCTCCAGCCCATCATCTTCTCCACATACGACGCCAAGAGCATGCCCCTGAAGAACGCGCGGCTCGCCGACATCTGCATCGGCACCTCCGCCGCCCCGACCTACCTCCCCGCGCACCACTTCCACACCCACGACGGCAACGGCAAGGAGCGCGAGTACAACCTCATCGACGGCGGCGTCGCCGCCAACAATCCGACGATGGTGGCGATGACGCAGATCACCAAGAAGATGATGGGCAAGGACAGGGAGGAGCTGTACCCGGTGAAGCCGTCGGACTGCGGCAAGTTCCTGGTGATGTCCGTCGGCACCGGCTCGACGTCCGACCAGGGGCTGTACACGGCGAAGCAGTGCTCCCAGTGGGGCATCATCAGCTGGCTCCGCAACAAGGGCATGGCGCCCATCATCGACATCTTCATGGCAGCCAGCTCCGACCTCGTCGACATCCACGCCGCCGTGCTCTTCCAGTCGCTGCACAGCGACGGCGACTACCTCCGCATCCAGGACAACTCGCTCCACGGCCCGGCGGCCACGGTGGACGCTGCCACGCCCGAGAACATGGCGGAGCTCCTCAGGATCGGCGAGCGGATGCTGGCGCAGAGGGTGTCCAGGGTGAACGTCGAGACCGGGAGGTACGAGGAGGTAAAGGGGGCCGGGAGCAACGCCGACGCGCTCGCCGGGTTCGCCAGGCAGCTCTCCGACGAGAGGAGGACAAGGCTCGAGCGCCGGCGTGGTGGCGCCGGCCGTCTGAAATCCAGACGCTGA
Protein Sequence
- >TraesCS4A02G018100.1
MASYWCRRPCESCSTRAMAGSVVGQPVAPGQRVTVLTIDGGGIRGIIPGTILDFLEAKLQELDGPGARLADYFDCIAGTSTGGLITAMITAPGKDGRPLFAARDVNRFYLDNGPYIFPQRRCALAAVTASLRRPRYSGKYLHGKIRSMLGETRLCDALTDVVIPTFDVKLLQPIIFSTYDAKSMPLKNARLADICIGTSAAPTYLPAHHFHTHDGNGKEREYNLIDGGVAANNPTMVAMTQITKKMMGKDREELYPVKPSDCGKFLVMSVGTGSTSDQGLYTAKQCSQWGIISWLRNKGMAPIIDIFMAASSDLVDIHAAVLFQSLHSDGDYLRIQDNSLHGPAATVDAATPENMAELLRIGERMLAQRVSRVNVETGRYEEVKGAGSNADALAGFARQLSDERRTRLERRRGGAGRLKSRR