Information report for OSIPA
Gene Details
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Functional Descriptions
- As such, the OSIPA promoter seems promising for generation of stable male-sterile lines required for the production of hybrids in rice and other crop plants.
- Earlier, a pollen-specific Oryza sativa indica pollen allergen gene (OSIPA), coding for expansins/pollen allergens, was isolated from rice, and its promoter–upon expression in tobacco and Arabidopsis–was found active during the late stages of pollen development.
- Pollen-specific expression of Oryza sativa indica pollen allergen gene (OSIPA) promoter in rice and Arabidopsis transgenic systems.
- Differential screening of a stage-specific cDNA library of Indica rice has been used to identify two genes expressed in pre-pollination stage panicles, namely OSIPA and OSIPK coding for proteins similar to expansins/pollen allergens and calcium-dependent protein kinases (CDPK), respectively.
- Northern analysis and in situ hybridizations indicate that OSIPA expresses exclusively in pollen while OSIPK expresses in pollen as well as anther wall.
- Promoter of OSIPA is active during the late stages of pollen development and remains active till the anthesis, whereas, OSIPK promoter is active to a low level in developing anther till the pollen matures.
Literature and News
- Pollen-specific expression of Oryza sativa indica pollen allergen gene (OSIPA) promoter in rice and Arabidopsis transgenic systems . DOI: 10.1007/s12033-010-9347-5 ; PMID: 21061188
- Promoters of two anther-specific genes confer organ-specific gene expression in a stage-specific manner in transgenic systems . DOI: 10.1007/s00299-007-0414-8 ; PMID: 17661051
Gene Resources
- UniProt: I3QD43
- EMBL: JF969198
- AlphaFoldDB: I3QD43
- EnsemblPlants: Os10t0548600-01
- Gramene: Os10t0548600-01
- KEGG: dosa:Os10g0548600
- Orthologous matrix: TWYGQPY
- InterPro: IPR005795, IPR007112, IPR007117
- PANTHER: PTHR31692, PTHR31692:SF7
- SUPFAM: SSF49590, SSF50685
- PROSITE: PS50842, PS50843
- Gene3D: 2.40.40.10, 2.60.40.760
- OrthoDB: I3QD43
- SWISS-MODEL: I3QD43
- Conserved Domain Database: cd22275
Sequences
cDNA Sequence
- >LOC_Os10g40090.1
ATCCATCTCACCACCAAAAAAAAAAGGAAAAAAAAACAAAACACACCAAGCCAAATAAAAGCGACAATGGGATCGCTCACCACCAACATCGTCCTCGCCGTCGCCGTGGTGGCAGCGCTGGTCGGCGGCGGGTCGTGCGGCCCGCCCAAGGTGCCACCCGGCCCGAACATCACGACCAACTACAACGCCCCGTGGCTCCCCGCCAGGGCCACCTGGTACGGCCAGCCCTACGGCTCCGGCTCCACCGACAATGGTGGCGCGTGCGGGATCAAGAACGTCAACCTGCCTCCCTACAACGGCATGATCTCCTGCGGCAACGTCCCAATCTTCAAGGACGGCAGGGGATGCGGCTCATGCTACGAGGTGAAGTGTGAGCAGCCGGCGGCGTGCTCGAAGCAGCCGGTGACGGTGTTCATCACGGACATGAACTACGAGCCCATCTCGGCGTACCACTTCGACTTCTCCGGCAAGGCGTTCGGCGCCATGGCTTGCCCGGGGAAGGAGACCGAGCTCCGCAAGGCCGGCATCATCGACATGCAGTTCAGGAGGGTGCGCTGCAAGTACCCCGGCGGCCAGAAGGTCACCTTCCACGTCGAGAAGGGCTCCAACCCCAACTACCTCGCCGTGCTCGTCAAGTTCGTCGCCGACGACGGTGACGTCATCCAGATGGACCTCCAGGAGGCCGGATTGCCAGCGTGGAGGCCCATGAAGCTGTCGTGGGGCGCCATCTGGAGGATGGACACCGCCACGCCACTCAAGGCACCCTTCTCCATTCGCGTCACCACCGAGTCCGGCAAGAGCCTCATCGCCAAAGACGTCATCCCGGTCAACTGGATGCCAGACGCCATCTACGTATCAAACGTCCAGTTCTATTGAGATCGGACGGAAACGATCCTCCTAATTTATTTCCCTATTAATTTGTTCAAATGGTTTCCTTCTATAACCTATATTTTTCCCGTTGTTAGAAATGGTTCCATTTCCTCCTACAGCTTACTTTAAGATAGTTGCGCTTGTATATCTGCGCCATCTTGTAAGTTGTAAGATGCTGAAGAACACTATGAATTCTGAGCATCTGATTCTCCGGGAAGATTTACTATGATAAACAACAGTTTGATTTACTATGTGTGTCCCCTTGTTTATTGTATGCTATCCTAATACTTATGAAAAGTTTTGATAAATA
CDS Sequence
- >LOC_Os10g40090.1
ATGGGATCGCTCACCACCAACATCGTCCTCGCCGTCGCCGTGGTGGCAGCGCTGGTCGGCGGCGGGTCGTGCGGCCCGCCCAAGGTGCCACCCGGCCCGAACATCACGACCAACTACAACGCCCCGTGGCTCCCCGCCAGGGCCACCTGGTACGGCCAGCCCTACGGCTCCGGCTCCACCGACAATGGTGGCGCGTGCGGGATCAAGAACGTCAACCTGCCTCCCTACAACGGCATGATCTCCTGCGGCAACGTCCCAATCTTCAAGGACGGCAGGGGATGCGGCTCATGCTACGAGGTGAAGTGTGAGCAGCCGGCGGCGTGCTCGAAGCAGCCGGTGACGGTGTTCATCACGGACATGAACTACGAGCCCATCTCGGCGTACCACTTCGACTTCTCCGGCAAGGCGTTCGGCGCCATGGCTTGCCCGGGGAAGGAGACCGAGCTCCGCAAGGCCGGCATCATCGACATGCAGTTCAGGAGGGTGCGCTGCAAGTACCCCGGCGGCCAGAAGGTCACCTTCCACGTCGAGAAGGGCTCCAACCCCAACTACCTCGCCGTGCTCGTCAAGTTCGTCGCCGACGACGGTGACGTCATCCAGATGGACCTCCAGGAGGCCGGATTGCCAGCGTGGAGGCCCATGAAGCTGTCGTGGGGCGCCATCTGGAGGATGGACACCGCCACGCCACTCAAGGCACCCTTCTCCATTCGCGTCACCACCGAGTCCGGCAAGAGCCTCATCGCCAAAGACGTCATCCCGGTCAACTGGATGCCAGACGCCATCTACGTATCAAACGTCCAGTTCTATTGA
Protein Sequence
- >LOC_Os10g40090.1
MGSLTTNIVLAVAVVAALVGGGSCGPPKVPPGPNITTNYNAPWLPARATWYGQPYGSGSTDNGGACGIKNVNLPPYNGMISCGNVPIFKDGRGCGSCYEVKCEQPAACSKQPVTVFITDMNYEPISAYHFDFSGKAFGAMACPGKETELRKAGIIDMQFRRVRCKYPGGQKVTFHVEKGSNPNYLAVLVKFVADDGDVIQMDLQEAGLPAWRPMKLSWGAIWRMDTATPLKAPFSIRVTTESGKSLIAKDVIPVNWMPDAIYVSNVQFY*