Information report for OsARF16
Gene Details
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Functional Descriptions
- In -Pi conditions, the shoot biomass of OsARF16 was slightly reduced, and neither root growth nor iron content was induced, indicating that the knockout of OsARF16 led to loss of response to Pi deficiency in rice.
- Six phosphate starvation-induced genes (PSIs) were less induced by -Pi in OsARF16 and these trends were similar to a knockdown mutant of OsPHR2 or AtPHR1, which was a key regulator under -Pi.
- OsARF16, a transcription factor, is required for auxin and phosphate starvation response in rice (Oryza sativa L.).
- Here, we show that OsARF16, an auxin response factor, functions in both auxin and -Pi responses in rice (Oryza sativa L.
- The knockout of OsARF16 led to primary roots (PR), lateral roots (LR) and root hair losing sensitivity to auxin and -Pi response.
- These data first reveal the biological function of OsARF16, provide novel evidence of a linkage between auxin and -Pi responses and facilitate the development of new strategies for the efficient utilization of Pi in rice.
- OsARF16 Is Involved in Cytokinin-Mediated Inhibition of Phosphate Transport and Phosphate Signaling in Rice (Oryza sativa L.).
- In this study, we found that OsARF16 is required for the cytokinin response and is involved in the negative regulation of Pi uptake and Pi signaling by cytokinin.
- Most Fe-deficient symptoms could be partially restored in the OsARF16 mutant, including dwarfism, photosynthesis decline, a reduction in iron content and root system architecture (RSA) regulation.
- Our results reveal a new biological function for OsARF16 and provide important information on how ARF-medicated auxin signaling affects iron signaling and the iron deficiency response.
- We found that OsARF16 was involved in the iron deficiency response and the induction of iron deficiency response genes.
- Furthermore, the qRT-PCR data indicated that the induction of Fe-deficiency response genes by iron deficiency was more compromised in the OsARF16 mutant than in Nipponbare.
- Strikingly, our genetic data showed that loss-of-function mutants of osarf12 or OsARF16 exhibit reduced resistance whereas osarf11 mutants display enhanced resistance to RDV.
- Transformation with modified OsARF11, OsARF16 and OsWOX3A genomic DNA lacking uORFs rescued the narrow leaf phenotype of nal21 to a better extent than transformation with their native genomic DNA, implying that RPS3A could regulate translation of ARFs and WOX3A through uORFs.
Functional Keywords
- growth , phosphate , auxin , iron , shoot , root-hair , biomass , pi , transcription-factor , lateral-root , primary-root , root , phosphate-transport , phosphate-signaling , cytokinin , cytokinin-response , Pi-uptake , Pi-signaling , architecture , photosynthesis , root-system-architecture , resistance , leaf
Literature and News
- OsARF16, a transcription factor, is required for auxin and phosphate starvation response in rice (Oryza sativa L.) . DOI: 10.1111/pce.12001 ; PMID: 22913536
- OsARF16 is involved in cytokinin-mediated inhibition of phosphate transport and phosphate signaling in rice (Oryza sativa L.) . DOI: 10.1371/journal.pone.0112906 ; PMID: 25386911
- OsARF16, a transcription factor regulating auxin redistribution, is required for iron deficiency response in rice (Oryza sativa L.) . DOI: 10.1016/j.plantsci.2014.12.003 ; PMID: 25576000
- Auxin response factors (ARFs) differentially regulate rice antiviral immune response against rice dwarf virus . DOI: 10.1371/journal.ppat.1009118 ; PMID: 33264360
- Narrow Leaf21, encoding ribosomal protein RPS3A, controls leaf development in rice . DOI: 10.1093/plphys/kiab075 ; PMID: 33591317
Gene Resources
- UniProt: A3B9A0
- EMBL: AP008212, AP014962, CM000143
- AlphaFoldDB: A3B9A0
- EnsemblPlants: Os06t0196700-02
- Gramene: Os06t0196700-02
- InterPro: IPR003340, IPR010525, IPR015300
- PANTHER: PTHR31384, PTHR31384:SF21
- SUPFAM: SSF101936, SSF54277
- PROSITE: PS50863, PS51745
- Gene3D: 2.30.30.1040, 2.40.330.10, 3.10.20.90
- OrthoDB: A3B9A0
- SWISS-MODEL: A3B9A0
- Conserved Domain Database: cd10017
- eggNOG: ENOG502QSK9
Sequences
cDNA Sequence
- >LOC_Os06g09660.1
ATGAAGGATCAGGGATCATCCGGTGTGTCTCCCGCCCCAGGGGAAGGGGAGAAGAAAGCCATCAATTCGGAGCTATGGCATGCTTGTGCCGGGCCTCTTGTGTCGCTGCCGCCGGTGGGCAGTCTCGTCGTGTACTTCCCTCAGGGTCATAGCGAGCAGGTTGCTGCTTCCATGCACAAGGAGCTGGACAACATCCCTGGTTATCCCTCTCTTCCGTCTAAGCTGATCTGCAAACTTCTGAGTCTCACCTTACATGCAGATTCTGAAACTGATGAAGTTTATGCTCAGATGACACTTCAACCAGTCAATAAATATGATCGAGATGCAATGCTGGCATCTGAACTGGGCCTGAAGCAAAACAAGCAACCAGCGGAGTTCTTTTGCAAAACGCTGACGGCGAGCGACACAAGTACCCATGGTGGATTTTCAGTGCCACGTCGTGCGGCGGAGAAGATATTTCCACCACTAGACTTTACCATGCAACCACCAGCACAAGAGCTCATCGCCAAGGATCTGCATGATATTTCATGGAAATTTCGACACATTTACCGAGGTCAACCAAAGAGGCACCTTCTGACAACTGGTTGGAGCGTCTTTGTCAGCACAAAAAGGCTTCTAGCTGGTGATTCAGTTCTGTTTATAAGGGATGAGAAATCTCAGCTTCTATTAGGCATACGTCGTGCTACCAGACCCCAACCAGCTCTATCGTCATCAGTTCTATCAAGTGATAGCATGCACATTGGGATTCTAGCTGCTGCAGCACATGCTGCTGCAAACAGTAGCCCATTTACTATTTTCTACAATCCAAGGGCAAGTCCATCAGAATTTGTCATTCCTTTAGCGAAATATAACAAGGCTTTGTATACACAAGTATCTCTTGGAATGCGGTTCAGAATGCTGTTTGAGACAGAGGATTCAGGGGTTCGAAGATATATGGGAACAATCACAGGTATTGGTGACTTGGATCCAGTGCGCTGGAAGAACTCTCATTGGCGAAACCTTCAGGTTGGTTGGGATGAATCAACAGCATCTGAGAGGCGCACTCGTGTTTCAATATGGGAGATTGAACCAGTCGCGACACCTTTTTATATTTGTCCACCACCATTTTTCAGGCCAAAACTTCCTAAGCAGCCAGGAATGCCAGATGATGAAAATGAAGTTGAGAGTGCTTTCAAAAGAGCCATGCCATGGCTTGCTGATGACTTTGCCCTGAAAGATGTGCAAAGTGCATTATTTCCAGGTCTGAGCCTAGTCCAATGGATGGCTATGCAACAGAATCCTCAGATGCTAACAGCTGCGTCCCAAACAGTGCAATCACCGTACTTGAACTCCAATGCATTGGCTATGCAGGATGTGATGGGTAGTAGCAACGAGGACCCAACAAAAAGATTGAACACACAGGCACAAAATATGGTTTTACCTAATTTACAGGTTGGCTCAAAAGTGGATCACCCTGTAATGTCTCAACATCAACAGCAGCCACACCAACTATCACAACAGCAGCAGGTCCAGCCATCGCAGCAAAGTTCTGTGGTTTTACAGCAACATCAAGCCCAGTTGCTGCAGCAGAACGCCATTCACTTGCAGCAGCAGCAAGAACATCTCCAGCGGCAGCAGTCACAACCGGCACAGCAGTTGAAGGCTGCTTCAAGTCTGCATTCAGTGGAACAGCACAAGCTGAAAGAACAGACTTCAGGTGGGCAGGTTGCCTCACAAGCACAAATGTTAAACCAGATTTTCCCACCATCTTCATCGCAGCTACAACAGTTAGGTTTACCCAAGTCACCTACTCATCGCCAAGGGTTGACAGGATTACCAATTGCAGGTTCTTTGCAGCAGCCCACACTAACTCAGACATCTCAAGTCCAGCAAGCAGCCGAATATCAGCAGGCCCTCCTACAGAGTCAGCAACAGCAACAGCAACTGCAACTGCAACAACTATCACAACCAGAAGTACAGCTGCAGCTGCTTCAAAAGATTCAACAACAAAACATGCTATCTCAGCTGAACCCACAACATCAGTCCCAGTTGATTCAACAATTGTCTCAGAAAAGCCAGGAAATTCTACAGCAACAAATTTTGCAACATCAATTTGGTGGGTCTGATTCTATTGGTCAACTCAAGCAATCACCATCGCAGCAAGCTCCTTTAAACCACATGACAGGATCTTTGACGCCCCAGCAACTTGTCAGATCACATTCGGCACTTGCTGAGAGTGGGGATCCATCCAGTTCAACTGCTCCATCCACCAGCCGTATTTCTCCAATAAATTCGCTGAGTAGGGCAAACCAAGGAAGCAGAAATTTAACTGACATGGTGGCAACACCACAAATTGACAACTTACTTCAGGAAATTCAAAGCAAGCCAGATAATCGAATTAAGAATGACATACAGAGCAAAGAAACAGTCCCTATACATAACCGACATCCAGTTTCTGATCAACTTGATGCATCATCTGCTACCTCCTTTTGTTTAGACGAGAGCCCACGAGAAGGTTTTTCCTTCCCTCCAGTTTGTTTGGATAACAATGTTCAAGTTGATCCAAGAGATAACTTTCTTATTGCGGAAAATGTGGACGCATTGATGCCAGATGCCCTGTTGTCAAGAGGTATGGCTTCAGGAAAGGGCATGTGCACTCTGACTTCTGGACAAAGGGATCACAGGGATGTCGAGAATGAGCTATCATCTGCTGCATTCAGTTCCCAGTCATTTGGTGTGCCTGACATGTCCTTTAAGCCTGGATGTTCAAGTGACGTTGCTGTTACTGATGCCGGAATGCCAAGCCAAGGTTTGTGGAATAATCAAACACAACGGATGAGAACTTTCACTAAGGTTCAAAAGCGTGGTTCTGTGGGGAGATCAATTGATATCACAAGATATCGAGATTATGATGAACTTAGGCATGATCTTGCATGCATGTTTGGTATCCAAGGTCAACTTGAAGATCCATATAGGATGGATTGGAAGCTAGTCTATGTTGATCATGAGAATGATATCCTTCTTGTCGGCGACGACCCTTGGGAGGAATTTGTGGGCTGTGTGAAGAGCATCAAAATACTCTCAGCTGCTGAAGTACAACAGATGAGCTTGGATGGTGACCTTGGTGGCGTCCCTCCACAAACACAGGCCTGTAGTGCCTCTGATGATGCAAATGCATGGAGAGGTTGA
CDS Sequence
- >LOC_Os06g09660.1
ATGAAGGATCAGGGATCATCCGGTGTGTCTCCCGCCCCAGGGGAAGGGGAGAAGAAAGCCATCAATTCGGAGCTATGGCATGCTTGTGCCGGGCCTCTTGTGTCGCTGCCGCCGGTGGGCAGTCTCGTCGTGTACTTCCCTCAGGGTCATAGCGAGCAGGTTGCTGCTTCCATGCACAAGGAGCTGGACAACATCCCTGGTTATCCCTCTCTTCCGTCTAAGCTGATCTGCAAACTTCTGAGTCTCACCTTACATGCAGATTCTGAAACTGATGAAGTTTATGCTCAGATGACACTTCAACCAGTCAATAAATATGATCGAGATGCAATGCTGGCATCTGAACTGGGCCTGAAGCAAAACAAGCAACCAGCGGAGTTCTTTTGCAAAACGCTGACGGCGAGCGACACAAGTACCCATGGTGGATTTTCAGTGCCACGTCGTGCGGCGGAGAAGATATTTCCACCACTAGACTTTACCATGCAACCACCAGCACAAGAGCTCATCGCCAAGGATCTGCATGATATTTCATGGAAATTTCGACACATTTACCGAGGTCAACCAAAGAGGCACCTTCTGACAACTGGTTGGAGCGTCTTTGTCAGCACAAAAAGGCTTCTAGCTGGTGATTCAGTTCTGTTTATAAGGGATGAGAAATCTCAGCTTCTATTAGGCATACGTCGTGCTACCAGACCCCAACCAGCTCTATCGTCATCAGTTCTATCAAGTGATAGCATGCACATTGGGATTCTAGCTGCTGCAGCACATGCTGCTGCAAACAGTAGCCCATTTACTATTTTCTACAATCCAAGGGCAAGTCCATCAGAATTTGTCATTCCTTTAGCGAAATATAACAAGGCTTTGTATACACAAGTATCTCTTGGAATGCGGTTCAGAATGCTGTTTGAGACAGAGGATTCAGGGGTTCGAAGATATATGGGAACAATCACAGGTATTGGTGACTTGGATCCAGTGCGCTGGAAGAACTCTCATTGGCGAAACCTTCAGGTTGGTTGGGATGAATCAACAGCATCTGAGAGGCGCACTCGTGTTTCAATATGGGAGATTGAACCAGTCGCGACACCTTTTTATATTTGTCCACCACCATTTTTCAGGCCAAAACTTCCTAAGCAGCCAGGAATGCCAGATGATGAAAATGAAGTTGAGAGTGCTTTCAAAAGAGCCATGCCATGGCTTGCTGATGACTTTGCCCTGAAAGATGTGCAAAGTGCATTATTTCCAGGTCTGAGCCTAGTCCAATGGATGGCTATGCAACAGAATCCTCAGATGCTAACAGCTGCGTCCCAAACAGTGCAATCACCGTACTTGAACTCCAATGCATTGGCTATGCAGGATGTGATGGGTAGTAGCAACGAGGACCCAACAAAAAGATTGAACACACAGGCACAAAATATGGTTTTACCTAATTTACAGGTTGGCTCAAAAGTGGATCACCCTGTAATGTCTCAACATCAACAGCAGCCACACCAACTATCACAACAGCAGCAGGTCCAGCCATCGCAGCAAAGTTCTGTGGTTTTACAGCAACATCAAGCCCAGTTGCTGCAGCAGAACGCCATTCACTTGCAGCAGCAGCAAGAACATCTCCAGCGGCAGCAGTCACAACCGGCACAGCAGTTGAAGGCTGCTTCAAGTCTGCATTCAGTGGAACAGCACAAGCTGAAAGAACAGACTTCAGGTGGGCAGGTTGCCTCACAAGCACAAATGTTAAACCAGATTTTCCCACCATCTTCATCGCAGCTACAACAGTTAGGTTTACCCAAGTCACCTACTCATCGCCAAGGGTTGACAGGATTACCAATTGCAGGTTCTTTGCAGCAGCCCACACTAACTCAGACATCTCAAGTCCAGCAAGCAGCCGAATATCAGCAGGCCCTCCTACAGAGTCAGCAACAGCAACAGCAACTGCAACTGCAACAACTATCACAACCAGAAGTACAGCTGCAGCTGCTTCAAAAGATTCAACAACAAAACATGCTATCTCAGCTGAACCCACAACATCAGTCCCAGTTGATTCAACAATTGTCTCAGAAAAGCCAGGAAATTCTACAGCAACAAATTTTGCAACATCAATTTGGTGGGTCTGATTCTATTGGTCAACTCAAGCAATCACCATCGCAGCAAGCTCCTTTAAACCACATGACAGGATCTTTGACGCCCCAGCAACTTGTCAGATCACATTCGGCACTTGCTGAGAGTGGGGATCCATCCAGTTCAACTGCTCCATCCACCAGCCGTATTTCTCCAATAAATTCGCTGAGTAGGGCAAACCAAGGAAGCAGAAATTTAACTGACATGGTGGCAACACCACAAATTGACAACTTACTTCAGGAAATTCAAAGCAAGCCAGATAATCGAATTAAGAATGACATACAGAGCAAAGAAACAGTCCCTATACATAACCGACATCCAGTTTCTGATCAACTTGATGCATCATCTGCTACCTCCTTTTGTTTAGACGAGAGCCCACGAGAAGGTTTTTCCTTCCCTCCAGTTTGTTTGGATAACAATGTTCAAGTTGATCCAAGAGATAACTTTCTTATTGCGGAAAATGTGGACGCATTGATGCCAGATGCCCTGTTGTCAAGAGGTATGGCTTCAGGAAAGGGCATGTGCACTCTGACTTCTGGACAAAGGGATCACAGGGATGTCGAGAATGAGCTATCATCTGCTGCATTCAGTTCCCAGTCATTTGGTGTGCCTGACATGTCCTTTAAGCCTGGATGTTCAAGTGACGTTGCTGTTACTGATGCCGGAATGCCAAGCCAAGGTTTGTGGAATAATCAAACACAACGGATGAGAACTTTCACTAAGGTTCAAAAGCGTGGTTCTGTGGGGAGATCAATTGATATCACAAGATATCGAGATTATGATGAACTTAGGCATGATCTTGCATGCATGTTTGGTATCCAAGGTCAACTTGAAGATCCATATAGGATGGATTGGAAGCTAGTCTATGTTGATCATGAGAATGATATCCTTCTTGTCGGCGACGACCCTTGGGAGGAATTTGTGGGCTGTGTGAAGAGCATCAAAATACTCTCAGCTGCTGAAGTACAACAGATGAGCTTGGATGGTGACCTTGGTGGCGTCCCTCCACAAACACAGGCCTGTAGTGCCTCTGATGATGCAAATGCATGGAGAGGTTGA
Protein Sequence
- >LOC_Os06g09660.1
MKDQGSSGVSPAPGEGEKKAINSELWHACAGPLVSLPPVGSLVVYFPQGHSEQVAASMHKELDNIPGYPSLPSKLICKLLSLTLHADSETDEVYAQMTLQPVNKYDRDAMLASELGLKQNKQPAEFFCKTLTASDTSTHGGFSVPRRAAEKIFPPLDFTMQPPAQELIAKDLHDISWKFRHIYRGQPKRHLLTTGWSVFVSTKRLLAGDSVLFIRDEKSQLLLGIRRATRPQPALSSSVLSSDSMHIGILAAAAHAAANSSPFTIFYNPRASPSEFVIPLAKYNKALYTQVSLGMRFRMLFETEDSGVRRYMGTITGIGDLDPVRWKNSHWRNLQVGWDESTASERRTRVSIWEIEPVATPFYICPPPFFRPKLPKQPGMPDDENEVESAFKRAMPWLADDFALKDVQSALFPGLSLVQWMAMQQNPQMLTAASQTVQSPYLNSNALAMQDVMGSSNEDPTKRLNTQAQNMVLPNLQVGSKVDHPVMSQHQQQPHQLSQQQQVQPSQQSSVVLQQHQAQLLQQNAIHLQQQQEHLQRQQSQPAQQLKAASSLHSVEQHKLKEQTSGGQVASQAQMLNQIFPPSSSQLQQLGLPKSPTHRQGLTGLPIAGSLQQPTLTQTSQVQQAAEYQQALLQSQQQQQQLQLQQLSQPEVQLQLLQKIQQQNMLSQLNPQHQSQLIQQLSQKSQEILQQQILQHQFGGSDSIGQLKQSPSQQAPLNHMTGSLTPQQLVRSHSALAESGDPSSSTAPSTSRISPINSLSRANQGSRNLTDMVATPQIDNLLQEIQSKPDNRIKNDIQSKETVPIHNRHPVSDQLDASSATSFCLDESPREGFSFPPVCLDNNVQVDPRDNFLIAENVDALMPDALLSRGMASGKGMCTLTSGQRDHRDVENELSSAAFSSQSFGVPDMSFKPGCSSDVAVTDAGMPSQGLWNNQTQRMRTFTKVQKRGSVGRSIDITRYRDYDELRHDLACMFGIQGQLEDPYRMDWKLVYVDHENDILLVGDDPWEEFVGCVKSIKILSAAEVQQMSLDGDLGGVPPQTQACSASDDANAWRG*