Gene Details:

• MSU gene ID: LOC_Os03g18550
• RAPdb gene ID: Os03g0296800
• Gene Symbol: MIT
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• In plants, iron (Fe) is essential for MITochondrial electron transport, heme, and Fe-Sulphur (Fe-S) cluster synthesis; however, plant MITochondrial Fe transporters have not been identified.
• Here we show, identify and characterize the rice MITochondrial Fe transporter (MIT).
• These results indicate that MIT is a MITochondrial Fe transporter essential for rice growth and development.
• The rice MITochondrial iron transporter is essential for plant growth.
• The screening of 3,993 mutant lines for symptoms of Fe deficiency resulted in the identification and characterization of a major MITochondrial Fe transporter (MIT) in rice.
• Identification and characterization of the major MITochondrial Fe transporter in rice.
• Here, we discuss the characterization of a rice vacuolar Fe transporter 2 (OsVIT2) T-DNA insertion line (osvit2) and report that the knockdown of OsVIT2 and MITochondrial Fe transporter (MIT) expression affects seed Fe localization.
• MIT localized to the MITochondria and complemented the growth of Deltamrs3Deltamrs4 yeast defective in MITochondrial Fe transport.
• The growth of MIT-knockdown (MIT-2) plants was also significantly impaired despite abundant Fe accumulation.
• MIT was found to localize to MITochondria and to complement the growth of a yeast strain defective in MITochondrial Fe transport.
• Further, the decrease in the activity of the MITochondrial and cytosolic Fe-S enzyme, aconitase, indicated that Fe-S cluster synthesis is affected in MIT-2 plants.
• The knock-out of MIT resulted in a lethal phenotype, and in knock-down plants, several agronomic characteristics were compromised, such as plant height, average number of tillers, days to flower, fertility, and yield.
• The knockdown of OsVIT2 and MIT affects iron localization in rice seed.
• Previously, we reported that the expression of OsVIT2 was higher in MIT knockdown (MIT-2) plants, and in this study, the accumulation of Fe in MIT-2 seeds decreased significantly.
• Moreover, changes in the expression of OsVIT2 and MIT affect the concentration and localization of metals in brown rice as well as in polished rice seeds.
• Despite rapid progress in our understanding of Fe homeostasis in plants, Fe transport from the cytoplasm to MITochondria was, until recently, poorly understood.
• Furthermore, the MITochondrial Fe concentration and the activity of the MITochondrial Fe-S enzyme aconitase were significantly reduced compared with wild-type plants.

Function-related keywords:

• transporter ,  growth ,  iron ,  mitochondria ,  tiller ,  yield ,  fertility ,  height ,  flower ,  seed

Literature:

• The knockdown of OsVIT2 and MIT affects iron localization in rice seed .  DOI:  10.1186/1939-8433-6-31 ;  PMID:  24280309
• The rice mitochondrial iron transporter is essential for plant growth .  DOI:  10.1038/ncomms1326 ;  PMID:  21610725
• Identification and characterization of the major mitochondrial Fe transporter in rice .  DOI:  10.4161/psb.6.10.17132 ;  PMID:  21921696
• Knocking down mitochondrial iron transporter (MIT) reprograms primary and secondary metabolism in rice plants .  DOI:  10.1093/jxb/erv531 ;  PMID:  26685186

Related News:

Gene Resources:

• NCBI ID: AK058471
• UniProt accessions:

Sequences: