Gene Details:

Functional Descriptions:

  • Activated OsRacGEF1 is required for chitin-driven immune responses and resistance to rice blast fungus infection.
  • Here, we show that rice OsRacGEF1 forms homodimers, and heterodimers with OsRacGEF2, at the plasma membrane (PM) and the endoplasmic reticulum (ER).
  • OsRacGEF2 does not bind directly to the receptor-like kinase (RLK) OsCERK1, but forms a complex with OsCERK1 through OsRacGEF1 at the ER.
  • In chitin signaling in rice, two direct substrates of OsCERK1, Rac/ROP GTPase guanine nucleotide exchange factor OsRacGEF1 and receptor-like cytoplasmic kinase OsRLCK185, have been identified as components of the OsCERK1 complex and are rapidly phosphorylated by OsCERK1 in response to chitin.
  • Knock-out and -down of OsRopGEF7B by T-DNA insertion and RNA interference, respectively, predominantly caused an increase in the number of floral organs in the inner whorls (stamen and ovary), as well as abnormal paleae/lemmas and ectopic growth of lodicules, resulting in decline of rice seed setting.
  • OsRopGEF7B plays roles in floral organ development in rice, affecting rice seed setting rate.
  • OsRopGEF7Bpro:GUS analysis indicates that OsRopGEF7B is expressed in various tissues, especially in the floral meristem and floral organ primordia.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os01g62990.1
    ATGGGGAGCGGGGAGGAGGAGAGGGAGGCGCGGAGCGAGGCGGCGTTCACGGACTCCGCCGACGGGAGCAGCTCCAGCTCCGACGCGGCCTCGGCCGACGAGTGGCCCGTGACGCTGGCGGCGCCGCCCAGGAAGACGGCGGCGTGCGGCCGCGTCCCCGGCGCGGAGGTGGTGGACAGCAGCAAGCCGCACGCGCAGAAGCGCAGAGCTCCCAGCTCAGAGATGGAGATGATGAAGGAGAGGTTCGCGAAGCTGCTGCTCGGGGAGGACATGTCCGGGAGCGGCAAGGGCGTGTGCACGGCGCTCGCCATTGCCAACGCCATTACCAACCTCTGCGCCACCATCTTCGGGCAGCTGTGGAGGCTGGAGCCACTCCCGCCGGAGAAGAAGGCCATGTGGCGGCGCGAGATGGGCTGGCTGCTCTGCGTCAGCGACCACATCGTCGAACTGGTCCCCACCTGGCAATCGTTCCCCGACGGAACCAGGCTCGAGGTCATGACAAGCAGACCACGATCTGATTTATACATCAATCTGCCAGCACTTCGGAAGCTAGACCATATGCTCATTGAAATCCTGGACAGCTTCAGAGACCCTGAGTTCTGGTACGTTGAACAAGGGATATGCGCACCGGATTGTGATGGCTCAGCTTCCTTCAGAGCAGCTTTCCACCGCCGTGATGAGAAATGGTGGTTGCCGGTGCCTCGTGTTCCTCCCGGAGGCCTGCGTGACAAAGCGAGGAAGCAACTGCAGCACAAACGTGACTGTGCTAATCAAATCTTGAAGGCTGCCTTGGCCATCAACAGCAATGCTCTAGCTGAAATGGAGGTCCCTGAATCGTATCTAGAATCCCTACCAAAGAATGGAAGGGCAACTTTGGGTGACATCATATACCGCTACATAACGTCTGATCATTTCTCTCCTGAGTGCCTTCTCGACTGCCTGGACCTGTCAACGGAGTACCAAGCACTGGAAATCGCCAACCGTGTCGAGGCATCAGTGTATGTGTGGCGCCGGAGGATTGCTGCAAAACCAGCAAGTGTCTTAGGCCGTGCCACCAGTGGCAGATCATCCTGGGGCATGGTGAAAGACATGATAATAGACACGGAGAAGAGGGAGCTGCTTGCCGAACGAGCAGAGGGCCTACTGATATGTTTGAAGCAAAGGTTTCCTGGACTGACGCAGACAAGCTTGGACATGAGCAAAATTCAGTATAACAGGGATGTGGGTAAATCAATACTGGAGAGTTATTCAAGGGTGCTGGAGAGTCTGGCGTCCAACATTGTCGCTCGCATCGACGACCTACTGAACATCGATGAACTGAACAGGCATGCAGAGCATTTTCCACAGGGTGATGCAGATTGCAGGATTGCTTGCAACAAGGCTGCTGTTCCACCGTACCAAGTGCCTGCCTCAGGCACACCGTTTGTGACCGCATATGCGACGCCCAGCTTCTCGCCGGCGCAGCTTGCAAGCCCATCAAAGAAAGAGAGGAGCCCACTGGGTGCAGGGAGACGGTCTTACAGCAACAGGGGGTTTGGCGCAAAGAAAGCCTTGGCAATTGATCTAGTTAATCCTGAAGTAATGGGGGTGATAATCAGCGGTGGTAAGATGATCGATGTCTCGACGACCACAGAGCTGTAA
CDS Sequence
  • >LOC_Os01g62990.1
    ATGGGGAGCGGGGAGGAGGAGAGGGAGGCGCGGAGCGAGGCGGCGTTCACGGACTCCGCCGACGGGAGCAGCTCCAGCTCCGACGCGGCCTCGGCCGACGAGTGGCCCGTGACGCTGGCGGCGCCGCCCAGGAAGACGGCGGCGTGCGGCCGCGTCCCCGGCGCGGAGGTGGTGGACAGCAGCAAGCCGCACGCGCAGAAGCGCAGAGCTCCCAGCTCAGAGATGGAGATGATGAAGGAGAGGTTCGCGAAGCTGCTGCTCGGGGAGGACATGTCCGGGAGCGGCAAGGGCGTGTGCACGGCGCTCGCCATTGCCAACGCCATTACCAACCTCTGCGCCACCATCTTCGGGCAGCTGTGGAGGCTGGAGCCACTCCCGCCGGAGAAGAAGGCCATGTGGCGGCGCGAGATGGGCTGGCTGCTCTGCGTCAGCGACCACATCGTCGAACTGGTCCCCACCTGGCAATCGTTCCCCGACGGAACCAGGCTCGAGGTCATGACAAGCAGACCACGATCTGATTTATACATCAATCTGCCAGCACTTCGGAAGCTAGACCATATGCTCATTGAAATCCTGGACAGCTTCAGAGACCCTGAGTTCTGGTACGTTGAACAAGGGATATGCGCACCGGATTGTGATGGCTCAGCTTCCTTCAGAGCAGCTTTCCACCGCCGTGATGAGAAATGGTGGTTGCCGGTGCCTCGTGTTCCTCCCGGAGGCCTGCGTGACAAAGCGAGGAAGCAACTGCAGCACAAACGTGACTGTGCTAATCAAATCTTGAAGGCTGCCTTGGCCATCAACAGCAATGCTCTAGCTGAAATGGAGGTCCCTGAATCGTATCTAGAATCCCTACCAAAGAATGGAAGGGCAACTTTGGGTGACATCATATACCGCTACATAACGTCTGATCATTTCTCTCCTGAGTGCCTTCTCGACTGCCTGGACCTGTCAACGGAGTACCAAGCACTGGAAATCGCCAACCGTGTCGAGGCATCAGTGTATGTGTGGCGCCGGAGGATTGCTGCAAAACCAGCAAGTGTCTTAGGCCGTGCCACCAGTGGCAGATCATCCTGGGGCATGGTGAAAGACATGATAATAGACACGGAGAAGAGGGAGCTGCTTGCCGAACGAGCAGAGGGCCTACTGATATGTTTGAAGCAAAGGTTTCCTGGACTGACGCAGACAAGCTTGGACATGAGCAAAATTCAGTATAACAGGGATGTGGGTAAATCAATACTGGAGAGTTATTCAAGGGTGCTGGAGAGTCTGGCGTCCAACATTGTCGCTCGCATCGACGACCTACTGAACATCGATGAACTGAACAGGCATGCAGAGCATTTTCCACAGGGTGATGCAGATTGCAGGATTGCTTGCAACAAGGCTGCTGTTCCACCGTACCAAGTGCCTGCCTCAGGCACACCGTTTGTGACCGCATATGCGACGCCCAGCTTCTCGCCGGCGCAGCTTGCAAGCCCATCAAAGAAAGAGAGGAGCCCACTGGGTGCAGGGAGACGGTCTTACAGCAACAGGGGGTTTGGCGCAAAGAAAGCCTTGGCAATTGATCTAGTTAATCCTGAAGTAATGGGGGTGATAATCAGCGGTGGTAAGATGATCGATGTCTCGACGACCACAGAGCTGTAA
Protein Sequence
  • >LOC_Os01g62990.1
    MGSGEEEREARSEAAFTDSADGSSSSSDAASADEWPVTLAAPPRKTAACGRVPGAEVVDSSKPHAQKRRAPSSEMEMMKERFAKLLLGEDMSGSGKGVCTALAIANAITNLCATIFGQLWRLEPLPPEKKAMWRREMGWLLCVSDHIVELVPTWQSFPDGTRLEVMTSRPRSDLYINLPALRKLDHMLIEILDSFRDPEFWYVEQGICAPDCDGSASFRAAFHRRDEKWWLPVPRVPPGGLRDKARKQLQHKRDCANQILKAALAINSNALAEMEVPESYLESLPKNGRATLGDIIYRYITSDHFSPECLLDCLDLSTEYQALEIANRVEASVYVWRRRIAAKPASVLGRATSGRSSWGMVKDMIIDTEKRELLAERAEGLLICLKQRFPGLTQTSLDMSKIQYNRDVGKSILESYSRVLESLASNIVARIDDLLNIDELNRHAEHFPQGDADCRIACNKAAVPPYQVPASGTPFVTAYATPSFSPAQLASPSKKERSPLGAGRRSYSNRGFGAKKALAIDLVNPEVMGVIISGGKMIDVSTTTEL*