Gene Details:

Functional Descriptions:

  • miR169o and ZmNF-YA13 act in concert to coordinate the expression of ZmYUC1 that determines seed size and weight in maize kernels.
  • We demonstrate that ZmNF-YA13 regulates the expression of the auxin biosynthetic gene ZmYUC1, which modulates auxin levels in the early developing seeds and determines the number of endosperm cells, thereby governing maize seed size and ultimately yield.
  • ZmNF-YA13 activates the transcription of maize auxin synthesis gene ZmYUC1.
  • Except for ZmYUC1, the expression levels of other auxin synthesis genes were extremely low, suggestive of a key role of ZmYUC1 in auxin synthesis at such an early stage of seed development.
  • Significant upregulation in ZmYUC1 in NF-YA13 OE plants and downregulation in miR169o OE were verified by qPCR in 5 DAP kernels.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Zm00001eb409250_T001
    CGTCAATGCACATAGCCCTGCTGTTTGTACTATAAATGGACCCACTCATCATCATAAGCAAACCAAGATCTCCTGACCACCAACATCTTTCCCATCCAGCTAGCAGCAGTTAATAAATTAAAAAGAGATGACGGAGAAGGTGCTAGTTCTGATCGTTGGTGCAGGTCCATCAGGCCTTGCTGTAGCAGCATGCCTCGGCGAGCACGGCATCCCGTACCACATTGTGGAGCGCGAGGACTGCAGCGCTTCGCTGTGGCGCAAACGCACGTACGATCGCCTCAAACTCCACCTCGCCAAGGAGTTCTGCGAGCTCCCTCGCATGTCGCATCCGAGCGACGCCCCCAAGTACATCACGAGGGAGCAGTTCGTGAGGTACGTCGACGACTACGTCGAGCGCTTCAACATCCTCCCCAGGTACAGCACCTCGGTCGAGTCCTGCGAGTACGACGAGGCCAGCGGCCGCTGGGACGTCAGAGCGCGTGACCTAGCGGACGGCGGCGGCCGAGTGGCCGAGTACACGACCAGGTTCTTGGTCGTAGCCACCGGCGAGAACTGCGAGGGAGTCATCCCGGATATCCCTGGGCTGCGCGATTTCCCGGCTGGTGAGGTCGTGCACTCGTCGAGCTACAAGTCGTGGAAGAACTATGCCGGGAAGAGAGTCCTGGTGGTTGGGTGTGGCAACTCTGGCATGGAGATTGCATATGATCTTGCCTACAATGGAGTGGAAACCTCCTTGGTTATCCGTAGCCCGGTGCATGTTATGACCAAGGGTCTGATTTACTTGGGCATGAGGCTGCTGAAATGGCACCTTCCCGTGAAACTTGTGGACTTCATCATCTTGACCTTGGCAAACATCCAGTTCGGTGACCTCTCCCGCTACGGCATAGTCAGGCCCGACATGGGCCCGCTTCTTCTCAAGGCCAAGACTGGCCGGTCAGCTGTCCTAGACGTTGGCACAACTCAGTTAATAAAGACTGGCGATATCAAGGTGGTTGGGGCAATATCTCGCATCAGAGGAAACACAGTGGAGTTCGAGGATGGGAAGGAGAGGGACTTCGATTCTCTCGTGTTCGCAACAGGATATAGAAGCACTGCGAACACGTGGCTTAAGGACGGTGGGAGCTTGCTAGACGACAATGGCATGCCGAAGAAGAAGGCGGCGGGGCCGCAGCAGGGCAGTCGGCCATGGAAGGGCGGCAACGGGCTCTACTGCGTTGGGCTGGGGATGGCTGGACTGGCTGGCATCTCTCGTGACGCAGTGAGCGTTGCTGCGGACATCAAGGCCGCGGTGGATTCCATGGTGGGGCCGCCGGCGTTCTGGTTCTGATCGAACAGCTTAGATCTATCTGCTAGCTCGATCGTCGATGCATGGACGGAGTGAAGAATAATAACGTTACGTACGTACGTACCGCGCGATCGAGAGGAGATCATCGATCTCTATCCATCCGACGTGTGGTGCGTGCGTGCGTGCTGCTGGTTAAGAATAATTAAGTAAGAGTGTTTGTTATTGGCTAGCTATAGCTAGCTTTGTGAACGTACCCTTTATTGTCTGTGTGTGCAGTGTGTGTGTTGTACCGTCGTTCCGCTAGCTCTGTGTTTGTTTCCTTGATAGCAGCTTTGTGTTTGTATATATGTGTGT
CDS Sequence
  • >Zm00001eb409250_T001
    ATGACGGAGAAGGTGCTAGTTCTGATCGTTGGTGCAGGTCCATCAGGCCTTGCTGTAGCAGCATGCCTCGGCGAGCACGGCATCCCGTACCACATTGTGGAGCGCGAGGACTGCAGCGCTTCGCTGTGGCGCAAACGCACGTACGATCGCCTCAAACTCCACCTCGCCAAGGAGTTCTGCGAGCTCCCTCGCATGTCGCATCCGAGCGACGCCCCCAAGTACATCACGAGGGAGCAGTTCGTGAGGTACGTCGACGACTACGTCGAGCGCTTCAACATCCTCCCCAGGTACAGCACCTCGGTCGAGTCCTGCGAGTACGACGAGGCCAGCGGCCGCTGGGACGTCAGAGCGCGTGACCTAGCGGACGGCGGCGGCCGAGTGGCCGAGTACACGACCAGGTTCTTGGTCGTAGCCACCGGCGAGAACTGCGAGGGAGTCATCCCGGATATCCCTGGGCTGCGCGATTTCCCGGCTGGTGAGGTCGTGCACTCGTCGAGCTACAAGTCGTGGAAGAACTATGCCGGGAAGAGAGTCCTGGTGGTTGGGTGTGGCAACTCTGGCATGGAGATTGCATATGATCTTGCCTACAATGGAGTGGAAACCTCCTTGGTTATCCGTAGCCCGGTGCATGTTATGACCAAGGGTCTGATTTACTTGGGCATGAGGCTGCTGAAATGGCACCTTCCCGTGAAACTTGTGGACTTCATCATCTTGACCTTGGCAAACATCCAGTTCGGTGACCTCTCCCGCTACGGCATAGTCAGGCCCGACATGGGCCCGCTTCTTCTCAAGGCCAAGACTGGCCGGTCAGCTGTCCTAGACGTTGGCACAACTCAGTTAATAAAGACTGGCGATATCAAGGTGGTTGGGGCAATATCTCGCATCAGAGGAAACACAGTGGAGTTCGAGGATGGGAAGGAGAGGGACTTCGATTCTCTCGTGTTCGCAACAGGATATAGAAGCACTGCGAACACGTGGCTTAAGGACGGTGGGAGCTTGCTAGACGACAATGGCATGCCGAAGAAGAAGGCGGCGGGGCCGCAGCAGGGCAGTCGGCCATGGAAGGGCGGCAACGGGCTCTACTGCGTTGGGCTGGGGATGGCTGGACTGGCTGGCATCTCTCGTGACGCAGTGAGCGTTGCTGCGGACATCAAGGCCGCGGTGGATTCCATGGTGGGGCCGCCGGCGTTCTGGTTCTGA
Protein Sequence
  • >Zm00001eb409250_P001
    MTEKVLVLIVGAGPSGLAVAACLGEHGIPYHIVEREDCSASLWRKRTYDRLKLHLAKEFCELPRMSHPSDAPKYITREQFVRYVDDYVERFNILPRYSTSVESCEYDEASGRWDVRARDLADGGGRVAEYTTRFLVVATGENCEGVIPDIPGLRDFPAGEVVHSSSYKSWKNYAGKRVLVVGCGNSGMEIAYDLAYNGVETSLVIRSPVHVMTKGLIYLGMRLLKWHLPVKLVDFIILTLANIQFGDLSRYGIVRPDMGPLLLKAKTGRSAVLDVGTTQLIKTGDIKVVGAISRIRGNTVEFEDGKERDFDSLVFATGYRSTANTWLKDGGSLLDDNGMPKKKAAGPQQGSRPWKGGNGLYCVGLGMAGLAGISRDAVSVAADIKAAVDSMVGPPAFWF

Source: Genetic information comes from the article