Gene Details:

Functional Descriptions:

  • Yellow stripe 1 (YS1) and ys3 are recessive mutants of maize (Zea mays L.) that result in symptoms typical of Fe deficiency, i.e., interveinal chlorosis of the leaves. The YS1 mutant is defective in the YS1 transporter and is therefore unable to take up Fe(III)–MAs complexes.
  • The homozygous seeds of Yellow stripe 1 (YS1) and ys3 mutant plants were used to identify the genes responsible for the YS1 and ys3 phenotypes, and to extend our understanding of Fe homeostasis in maize.
  • In agreement with previous reports, the expression level of YS1 was decreased in the YS1 mutant. Moreover, we identified that the expression level of a homolog of TOM1 in maize (ZmTOM1) was significantly decreased in the ys3 mutant.
  • Immunostaining showed that ZmYS1 was localized in the epidermal cells of both crown and lateral roots, with a polar localization at the distal side of the epidermal cells. In maize leaves, ZmYS1 was localized in mesophyll cells, but not epidermal cells.
  • These differences in gene expression pattern and tissue-type specificity of localization suggest that HvYS1 is only involved in primary Fe acquisition by barley roots, whereas ZmYS1 is involved in both primary Fe acquisition and intracellular transport of iron and other metals in maize.
  • The gene Yellow stripe1 (Ys1) encodes the Fe(III)-PS transporter of maize (Zea mays).
  • YS1 is able to translocate iron that is bound either by PS or by the related compound, nicotianamine; thus, the role of YS1 may be to transport either of these complexes. Ys1 expression at both the mRNA and protein levels responds rapidly to changes in iron availability but is not strongly affected by limitation of copper or zinc.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Zm00001eb249020_T001
    GGACTAATGATTGTAAGTGAGATTTTTAGATAACGAGTTGTTCTAAGTGAGATGGCATGTGTGGTTGACATTTGAAGGATTTCTCTATATAAATCCCGGCTACTATTAACATCTCAACCTCACCATCTTCTCTTGATCGAGGCAGGGATCAAGCGACCGAGACCCGCCATGGACCTTGCACGGAGCGGCGGTGCCGCTGCCGCGGACGACGGGGGGGAGATCGAGAGGCACGAGCCGGCGCCCGAGGACATGGAGTCCGACCCCGCGGCGGCGCGCGAGAAGGAGCTGGAGCTGGAGCGGGTGCAGCCGTGGCGGGAGCAGGTGACCCTGCGCGGCGTGGTGGCGGCGCTGCTGATCGGCTTCATGTACAGCGTGATCGTGATGAAGATCGCGCTGACCACGGGGCTGGTGCCCACGCTGAACGTCTCCGCGGCGCTGATGGCGTTCCTGGCGCTCCGCGGGTGGACGCGCGTGCTGGAGCGCCTCGGCGTGGCGCACCGCCCCTTCACGCGCCAGGAGAACTGCGTCATCGAGACCTGCGCCGTCGCGTGCTACACCATCGCGTTCGGTGGTGGGTTCGGCTCCACGCTGCTGGGCCTGGACAAGAAGACGTACGAGCTGGCCGGGGCCTCGCCGGCCAACGTCCCGGGCAGCTACAAGGACCCTGGGTTCGGCTGGATGGCCGGGTTCGTCGCGGCGATCAGCTTCGCCGGCCTCCTAAGCCTGATCCCCCTCAGAAAGGTTCTGGTCATTGACTACAAGCTAACTTACCCAAGCGGGACTGCGACCGCTGTTCTTATAAACGGGTTCCACACCAAGCAAGGAGACAAGAACGCAAGGATGCAAGTCCGAGGGTTCCTCAAGTACTTTGGGCTCAGCTTCGTGTGGAGCTTTTTCCAGTGGTTCTACACAGGCGGTGAAGTTTGCGGCTTTGTTCAGTTTCCTACGTTCGGTCTGAAGGCCTGGAAGCAGACGTTCTTCTTTGATTTTAGCCTCACGTACGTTGGTGCGGGGATGATCTGTTCGCACCTCGTGAACATCTCCACCCTCCTTGGTGCCATCCTGTCGTGGGGGATACTGTGGCCACTCATCAGCAAGCAGAAAGGGGAGTGGTACCCTGCGAACATACCTGAGAGTAGCATGAAAAGCTTATACGGTTACAAGGCATTCCTCTGCATAGCTCTGATCATGGGAGACGGTACATACCACTTCTTTAAAGTCTTCGGTGTCACTGTTAAGAGTCTGCATCAACGGCTGAGCCGCAAACGTGCTACCAACAGAGTGGCAAACGGTGGAGACGAAATGGCCGCGCTTGACGACCTACAGCGTGACGAGATCTTCAGCGACGGGTCTTTCCCCGCCTGGGCAGCTTACGCCGGGTACGCCGCGCTGACCGTCGTCTCAGCGGTCATCATCCCGCACATGTTCCGGCAGGTCAAGTGGTACTACGTGATCGTGGCCTACGTCCTCGCCCCTCTCCTCGGCTTCGCCAACTCCTACGGCACGGGGCTCACCGACATCAACATGGCCTACAACTACGGCAAGATCGCGCTCTTCATCTTCGCGGCCTGGGCCGGCAGGGACAACGGCGTCATCGCGGGCCTCGCCGGCGGCACCCTGGTGAAGCAGCTGGTGATGGCGTCCGCGGACCTGATGCACGACTTCAAGACGGGCCACCTGACCATGACGTCGCCCAGGTCCCTGCTCGTGGCGCAGTTCATCGGGACGGCCATGGGCTGCGTCGTCGCGCCCCTCACGTTCCTGCTCTTCTACAACGCGTTCGACATCGGGAACCCCACCGGGTACTGGAAGGCGCCGTACGGCCTCATCTACCGCAACATGGCGATCCTCGGCGTGGAGGGCTTCTCCGTGCTGCCCAGGCACTGCCTCGCGCTCTCCGCTGGGTTCTTCGCGTTCGCCTTCGTCTTCAGCGTCGCCCGGGACGTCCTGCCGCGGAAGTACGCCAGGTTCGTGCCCCTGCCCATGGCCATGGCCGTGCCGTTCCTCGTGGGCGGGAGCTTCGCGATCGATATGTGCGTCGGGAGCCTGGCCGTCTTTGTCTGGGAGAAGGTGAACAGGAAGGAGGCCGTGTTCATGGTGCCTGCGGTTGCGTCCGGTTTGATCTGTGGAGACGGCATATGGACCTTCCCGTCTTCCATTCTCGCTCTGGCCAAGATCAAGCCACCGATTTGCATGAAGTTCACTCCTGGAAGCTAGAAACAGTATTGACAGTTCACAGAAATGGAGAATGCGAGATACACTGAAGGCATCAACTGTGGTTGGTTGAAGTGTTGAAGAGCAAGTCCTCTGGTCGTCGACATGAAGCGGAGCCATATGTCCCCTGTATTATCCTTGTTGTGCATGTGTGGGTTCTCCAAGGGCTACAGCACTCGAGATTTTCTATAGAGTTGATCATATGTTTTGTTGTCATGTTGGGTTCAACCTATGTTTATGTTTTGGGTGGAAAAAAGATACAACTGTACATTTTTAACAGAAATTAATGCTCATTTTGGTTGTGTATTTAC
CDS Sequence
  • >Zm00001eb249020_T001
    ATGGACCTTGCACGGAGCGGCGGTGCCGCTGCCGCGGACGACGGGGGGGAGATCGAGAGGCACGAGCCGGCGCCCGAGGACATGGAGTCCGACCCCGCGGCGGCGCGCGAGAAGGAGCTGGAGCTGGAGCGGGTGCAGCCGTGGCGGGAGCAGGTGACCCTGCGCGGCGTGGTGGCGGCGCTGCTGATCGGCTTCATGTACAGCGTGATCGTGATGAAGATCGCGCTGACCACGGGGCTGGTGCCCACGCTGAACGTCTCCGCGGCGCTGATGGCGTTCCTGGCGCTCCGCGGGTGGACGCGCGTGCTGGAGCGCCTCGGCGTGGCGCACCGCCCCTTCACGCGCCAGGAGAACTGCGTCATCGAGACCTGCGCCGTCGCGTGCTACACCATCGCGTTCGGTGGTGGGTTCGGCTCCACGCTGCTGGGCCTGGACAAGAAGACGTACGAGCTGGCCGGGGCCTCGCCGGCCAACGTCCCGGGCAGCTACAAGGACCCTGGGTTCGGCTGGATGGCCGGGTTCGTCGCGGCGATCAGCTTCGCCGGCCTCCTAAGCCTGATCCCCCTCAGAAAGGTTCTGGTCATTGACTACAAGCTAACTTACCCAAGCGGGACTGCGACCGCTGTTCTTATAAACGGGTTCCACACCAAGCAAGGAGACAAGAACGCAAGGATGCAAGTCCGAGGGTTCCTCAAGTACTTTGGGCTCAGCTTCGTGTGGAGCTTTTTCCAGTGGTTCTACACAGGCGGTGAAGTTTGCGGCTTTGTTCAGTTTCCTACGTTCGGTCTGAAGGCCTGGAAGCAGACGTTCTTCTTTGATTTTAGCCTCACGTACGTTGGTGCGGGGATGATCTGTTCGCACCTCGTGAACATCTCCACCCTCCTTGGTGCCATCCTGTCGTGGGGGATACTGTGGCCACTCATCAGCAAGCAGAAAGGGGAGTGGTACCCTGCGAACATACCTGAGAGTAGCATGAAAAGCTTATACGGTTACAAGGCATTCCTCTGCATAGCTCTGATCATGGGAGACGGTACATACCACTTCTTTAAAGTCTTCGGTGTCACTGTTAAGAGTCTGCATCAACGGCTGAGCCGCAAACGTGCTACCAACAGAGTGGCAAACGGTGGAGACGAAATGGCCGCGCTTGACGACCTACAGCGTGACGAGATCTTCAGCGACGGGTCTTTCCCCGCCTGGGCAGCTTACGCCGGGTACGCCGCGCTGACCGTCGTCTCAGCGGTCATCATCCCGCACATGTTCCGGCAGGTCAAGTGGTACTACGTGATCGTGGCCTACGTCCTCGCCCCTCTCCTCGGCTTCGCCAACTCCTACGGCACGGGGCTCACCGACATCAACATGGCCTACAACTACGGCAAGATCGCGCTCTTCATCTTCGCGGCCTGGGCCGGCAGGGACAACGGCGTCATCGCGGGCCTCGCCGGCGGCACCCTGGTGAAGCAGCTGGTGATGGCGTCCGCGGACCTGATGCACGACTTCAAGACGGGCCACCTGACCATGACGTCGCCCAGGTCCCTGCTCGTGGCGCAGTTCATCGGGACGGCCATGGGCTGCGTCGTCGCGCCCCTCACGTTCCTGCTCTTCTACAACGCGTTCGACATCGGGAACCCCACCGGGTACTGGAAGGCGCCGTACGGCCTCATCTACCGCAACATGGCGATCCTCGGCGTGGAGGGCTTCTCCGTGCTGCCCAGGCACTGCCTCGCGCTCTCCGCTGGGTTCTTCGCGTTCGCCTTCGTCTTCAGCGTCGCCCGGGACGTCCTGCCGCGGAAGTACGCCAGGTTCGTGCCCCTGCCCATGGCCATGGCCGTGCCGTTCCTCGTGGGCGGGAGCTTCGCGATCGATATGTGCGTCGGGAGCCTGGCCGTCTTTGTCTGGGAGAAGGTGAACAGGAAGGAGGCCGTGTTCATGGTGCCTGCGGTTGCGTCCGGTTTGATCTGTGGAGACGGCATATGGACCTTCCCGTCTTCCATTCTCGCTCTGGCCAAGATCAAGCCACCGATTTGCATGAAGTTCACTCCTGGAAGCTAG
Protein Sequence
  • >Zm00001eb249020_P001
    MDLARSGGAAAADDGGEIERHEPAPEDMESDPAAAREKELELERVQPWREQVTLRGVVAALLIGFMYSVIVMKIALTTGLVPTLNVSAALMAFLALRGWTRVLERLGVAHRPFTRQENCVIETCAVACYTIAFGGGFGSTLLGLDKKTYELAGASPANVPGSYKDPGFGWMAGFVAAISFAGLLSLIPLRKVLVIDYKLTYPSGTATAVLINGFHTKQGDKNARMQVRGFLKYFGLSFVWSFFQWFYTGGEVCGFVQFPTFGLKAWKQTFFFDFSLTYVGAGMICSHLVNISTLLGAILSWGILWPLISKQKGEWYPANIPESSMKSLYGYKAFLCIALIMGDGTYHFFKVFGVTVKSLHQRLSRKRATNRVANGGDEMAALDDLQRDEIFSDGSFPAWAAYAGYAALTVVSAVIIPHMFRQVKWYYVIVAYVLAPLLGFANSYGTGLTDINMAYNYGKIALFIFAAWAGRDNGVIAGLAGGTLVKQLVMASADLMHDFKTGHLTMTSPRSLLVAQFIGTAMGCVVAPLTFLLFYNAFDIGNPTGYWKAPYGLIYRNMAILGVEGFSVLPRHCLALSAGFFAFAFVFSVARDVLPRKYARFVPLPMAMAVPFLVGGSFAIDMCVGSLAVFVWEKVNRKEAVFMVPAVASGLICGDGIWTFPSSILALAKIKPPICMKFTPGS

Source: Genetic information comes from the article