Gene Details:
- Gene ID: Zm00001eb079410
- Gene Symbol: ZmPIMT2
- Gene Name: protein-L-isoaspartate methyltransferase2
- Genome: Zm-B73-REFERENCE-NAM-5.0
- Species: Zea mays
Functional Descriptions:
- The YFP signal in NZmPIMT2:YFP expression vector-transformed protoplasts exhibited a similar subcellular pattern as seen with the ZmPIMT2:YFP expression vector , suggesting that the N-terminus of ZmPIMT2 is essential for the mitochondrial and chloroplast localization of ZmPIMT2.
- Overexpression of ZmPIMT2 in maize and Arabidopsis thaliana increased seed vigor upon artificial aging.
- ZmPIMT2 positively affects maize seed vigor.
- ZmPIMT2 binds ZmMCCα in mitochondria, repairs isoAsp damage, and positively affects maize seed vigor.
- ZmPIMT2 is specifically expressed in the maize embryo. Both mRNA and protein levels of ZmPIMT2 increased during seed maturation and declined during imbibition.
- ZmPIMT2 binding to ZmMCCα was confirmed by luciferase complementation tests in both tobacco (Nicotiana benthamiana) leaves and maize protoplasts.
- The N-terminus of ZmPIMT2 is essential for its mitochondrial localization and function.
Function-related keywords:
- chloroplast , seed , mitochondria , embryo , protoplasts
Literature:
- Maize PIMT2 repairs damaged 3-METHYLCROTONYL COA CARBOXYLASE in mitochondria, affecting seed vigor. DOI: 10.1111/tpj.16225 ; PMID: 36999611
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >Zm00001eb079410_T001
CCCTGAACGGCCCACAGAGGCCCAGTTGCGTAGGCACTTTCCACTGTGAGTTGTGACGCCGCCGTCCCTGGCTCCTTGCTGCGTGCTGCCCTTGTGCAGTTGTGCTCCTTCCTTCCTTGCCCCGTACCCCGCCGGCGCGCCCTGAGCCAAATCGCAGGCTGCCATTGTGCCATGGACGAAGGTGCGACGACGAGCGACTCGTCCGCTGCCGAGACATTTGATCCGGACCTGATTCACGCCATCTTCAAGCTGGTGTGGAGGCGGCGGGCGGGGAAGGGGGCCGGCGGCGGCGGCAGCATTGAGGACATCGATGCGGAGCGTGCTCCAGAGACATCGAGGAGAAATCGGAGCACAACTGCCAATGCGACTGCTCTTAAAGTCAGCTGCGAACTTCTACGGATATTTGTAACAGAGGCTATTCAACGCTCTGCTTTTATCGCTGAAGCGGAGGATGGTGCTGTTATTGAACCCACCCACCTAGAGCGTGTGTTGCCGCAGCTGCTCTTGGACTTCTGAGGTACCCCATGGAGCCTCCAAAAGCATTTATCTAGGCGTGTCTGCTATGGAAGGATACAGATGCCTGGATGCATCAACAGAATCTTATTGCTGATCTATGTCTTGTAATCTGTAAACACACGAGATTTCTATATGGCTTTATAAAAATGCATGCATTTCTTGGAATGTTTTCCTGTTCGTGTTGTGTTTGACGAGATTATTGCCATTATAAAACACGACAGAGTTGGTTTCGATGAAATGGAGATGTAGGGAAGGGCTTCGACGGTTTGGAGGCGCAGACGACTGCGAAGCGCTGATATAGACATGAAAATCCCATTAGTTGTTACGGACACTAATTGATCTCCCGCTGTGAGAATAAGTCTCCTTAACCAGGTTGCCGTCTTCAGCGCCGTCATCCCCAGGCTGCGGTGCCGGACTCGGACGACGCGGTGCTGCTCGACGTCCTCAATCGCATCGGGGACGTCAAGGCGCTCGGCCGCTGCGCCCTCGTCTCGCGCCGCTTCCACGCACTCGTGCCGCTCGTCTGCTCCGTCTTCGTCCGCGTCGACGAGCCCACCTCCGGCCTCGACAACGCCTCCGCGCTCCACATCGTCGGCGCGCTCCGCGCCATGGCCGAGGCGCGCGGACGCACAGTGCTGCTCAGCATCCACCAGCCGGGAGCGTGCATCGTCAAGATGTTCGACTCCGTCCTCCTCCTAGCCGTCGGCTCCGTCCTCCACCATGGCATCGTCGACCAGCTCCGCGCCCTGCTCGGGGACGCGGGCCTCCACCTGCCCCCGCACGTCGACGACGTCGAGTTCGCCATCGACTCCGTGGACGCGCTCCGCCGCCACGCACGTCGTCCTCCGGAGACCGAGAGGGCTGCAGCGTCTGTTCCAGCTGCACGGCAAGCAGGTGGCGGACGCCCCCGTGACCATGATGGCTAGCCGCTACGCCAACTCGAGGGCGCGGGAGGTGGCGGTGTGGCTGCGCTTCTTCAAGAACGTGGCGCGGACGCGACAGCTCTTCGCGTGCCGCACCGTGTGCATGCTGGTGGCGGGCCTTGCGCTGGGCTCCATCTTCTACGACCTCGGGGCAGGACAAGGTGGCGGAGCGCGTGGGTTTCTCCGCCTTCCTGCTCACCTTCCTCCTGTCATCCACCACGGAGGCGCTGCCCATCTTCCTGCAGGAGCGCGACATCTTGGCCAGGGAGGCGGCGTCGGGGGCGTCGAGTCCGGCACCGCGGCCTGGGGATGACGGCGCTGAAGACGGCACTGGCTCAGGAGACTTATTCTCACGCGGGAGATTAATTAGTGTCCGTAACAACTAATGGGGTTTTTATGTCCATATCAGTGTTTCGCCGTCGTCTGCGTCAAACTGTCGAAGTCCTTACCTATACCTCTATTTCGTTGACACAACGAAACGCAGCCCAGAAGTCATCACCGCGCTGGAGCTCCGGGTGCTAACAATCTGGGCCTGCAGTATTTAAAAACTCGCCGGCGCTGGCAGAGTGGCAGGCTTTGCAACTACAAGGGCCGTGGCCGGTGTCCCGTGGCGGTAGAACGCGTGCGTCGGCAGGAGCCCCCCCACCTGTCGAGACGAGGCTTCTCCAAACCGCCACCGCAGCCGACCCCTGCTGCCCCGGCTTTGTTATAACGGATTTTTTTCCCGACACGGCTGGTCGGCTGCCATCGCCATGTGCCTCGCCGCCGCGTGCGCGTCCGCCACCCTAGCCCGCCGCCTCTCGCCCTCCACCGCGCTCCCGCGCCGCTTCCTCCTCGGCGCCCCCACGAGGCCTTCCCCGCCACCGCCTCTGCTGCGGCGCTGCCTCCCGTTCCACTTCTACCATATGGCGCAATATTGGACTCAACCATCACTGGACAGGAACAAAGCTATGGTTGAGTACCTAAAGCAGTATGGTGCTGTTAGAACTGATAAAGTGAAGGAAGTAATGGAAACGATTGATAGAGCACTATTTGTACCAGAGGGTACCCCTTACATTGACAGTCCTATGCCTATTGGTTTCAACGCGACGATATCTGCTCCTCACATGCATGCGACCTGCTTAGAGCTATTGAAGGATCATTTGCAACCAGGAATGCATGCTCTGGATGTCGGATCAGGTAGTGGCTACCTGACAGCTTGTTTTGCCATGATGGTTGGACCAGAAGGCCGTGCAGTGGGAATTGAACACGTTCCCGAGATCGTTGCTTCTTCTGTTGAAAATGTGCAGAGAAGTGCTGCAGCTCCACTACTGAGGGATGGATCGCTCTCTTTTCATGTTACAGATGGCAGGCTTGGCTTTCCAGACGCGGCACCCTACGACGCTATCCATGTTGGTGCAGCGGCACCTGAGATCCCTCAGCCTCTGCTTGATCAGCTAAAGCCTGGCGGCCGGATGGTGATACCGGTTGGCACCTACTCCCAGGACC
CDS Sequence
- >Zm00001eb079410_T001
ATGTGCCTCGCCGCCGCGTGCGCGTCCGCCACCCTAGCCCGCCGCCTCTCGCCCTCCACCGCGCTCCCGCGCCGCTTCCTCCTCGGCGCCCCCACGAGGCCTTCCCCGCCACCGCCTCTGCTGCGGCGCTGCCTCCCGTTCCACTTCTACCATATGGCGCAATATTGGACTCAACCATCACTGGACAGGAACAAAGCTATGGTTGAGTACCTAAAGCAGTATGGTGCTGTTAGAACTGATAAAGTGAAGGAAGTAATGGAAACGATTGATAGAGCACTATTTGTACCAGAGGGTACCCCTTACATTGACAGTCCTATGCCTATTGGTTTCAACGCGACGATATCTGCTCCTCACATGCATGCGACCTGCTTAGAGCTATTGAAGGATCATTTGCAACCAGGAATGCATGCTCTGGATGTCGGATCAGGTAGTGGCTACCTGACAGCTTGTTTTGCCATGATGGTTGGACCAGAAGGCCGTGCAGTGGGAATTGAACACGTTCCCGAGATCGTTGCTTCTTCTGTTGAAAATGTGCAGAGAAGTGCTGCAGCTCCACTACTGAGGGATGGATCGCTCTCTTTTCATGTTACAGATGGCAGGCTTGGCTTTCCAGACGCGGCACCCTACGACGCTATCCATGTTGGTGCAGCGGCACCTGAGATCCCTCAGCCTCTGCTTGATCAGCTAAAGCCTGGCGGCCGGATGGTGATACCGGTTGGCACCTACTCCCAGGAC
Protein Sequence
- >Zm00001eb079410_P001
MCLAAACASATLARRLSPSTALPRRFLLGAPTRPSPPPPLLRRCLPFHFYHMAQYWTQPSLDRNKAMVEYLKQYGAVRTDKVKEVMETIDRALFVPEGTPYIDSPMPIGFNATISAPHMHATCLELLKDHLQPGMHALDVGSGSGYLTACFAMMVGPEGRAVGIEHVPEIVASSVENVQRSAAAPLLRDGSLSFHVTDGRLGFPDAAPYDAIHVGAAAPEIPQPLLDQLKPGGRMVIPVGTYSQD
Source: Genetic information comes from the article