Information report for ZmMPK3;gpm443
Gene Details
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Functional Descriptions
- A novel MAPK gene, ZmMPK3, from ABA-treated maize leaves cDNA was isolated and characterized. The full length of the ZmMPK3 gene is 1 520 bp and encodes a 376 amino acid protein with a predicted molecular mass of 43.5 kD and a pI of 5.83.
- ZmMPK3 contains all 11 MAPK conserved subdomains and the phosphorylation motif TEY. Amino acid sequence alignment revealed that ZmMPK3 shared high identity with group-A MAPK in plants.
- ABA and H2O2 induced a significant increase in the ZmMPK3 activity using immunoprecipitation and in-gel kinase assay. Furthermore, the results showed that the ZmMPK3 protein is localized mainly to the nucleus. These results suggest that the ZmMPK3 may play an important role in response to environmental stresses.
Functional Keywords
- kinase , nucleus , aba , protein-kinase , pi
Literature and News
Gene Resources
- NCBI ID: [EU130900 LOC100272353](https://www.ncbi.nlm.nih.gov/gene/?term=EU130900 LOC100272353)
- UniProt accessions: A0A1D6JYY1
- EMBL: CM007647
- AlphaFoldDB: A0A1D6JYY1
- InterPro: IPR000719 , IPR008271 , IPR011009 , IPR050117
- PANTHER: PTHR24055
- SUPFAM: SSF56112
- PROSITE: PS00108 , PS50011
- Gene3D: 1.10.510.10 , 3.30.200.20
Sequences
cDNA Sequence
- >Zm00001eb013100_T001
GTCCTCCCATCAGCGCCGTCCGTAGCGGAGCAGTGCCAGTGGCCGTGCCGTGCGTCACGCACAAGGCACACGGCCCCCCGCCCGGTCGTGGGCAGAGCACCCGGCGGCAGCGATCGAGGCTTTGCTCCTGCTTCGGCTTCAGCCCCCCCAATTCTTTATAAAAATCCCTCGCCAGCGCTTCCCTCTTTTGACAAATCGGCCGCGGGGGAGAGAGATATATAGAGCCAATTTTTGCCCGATTTGCCTGTCTGCTCGGGACTGAGCCGGTATTCCTCTTCCTCGTCGACGAGAATAAATAAGCTAAGCCAGGGCCCCGGGGAAGAAGAAGATGAGCTCCGCCGGAGGCATGGACGGGTCTCCGCCGGTCGCCGAATTCCAGCCGACGGTGACGCACGGCGGCCGGTTCCTCCGGTACAATATCTTCGGCAACCTGTTCGAGATCACGCGCAAGTACCAGCCTCCCGTCATGCCCATCGGCCGCGGCGCCTACGGGATCGTCTGCTCGGTGATGAACTTCGAGACGAGGGAGATGGTGGCCATCAAGAAGATCGCCAACGCCTTCGACAACCACATGGACGCCAAGCGCACGCTCCGGGAGATCAAGCTGATGCGGCACCTCGACCACGAGAACATCATCGGCATCAGGGACGTGATCCCGCCGCCGATCCCGCGGGCTTTCAACGACGTGTACATCGGCATGGAGTTCATGGAGACGGACCTCCACCAGATCATCCGCTCCGGGCAGGAGCTGTCGGAGGAGCACTGCCAGTACTTCCTGTACCAGATCCTGCGCGGGCTCAAGTACATCCACTCCGCCGGCGTGATCCACCGCGACCTCAAGCCCAGCAACCTGCTGCTCAGCGCCAACTGCGACCTCAAGATCTGCGACTTCGGGCTGGCGCGCCCGTCCTCCGACAGCGACATGATGACGGAGTACGTGGTGACGCGGTGGTACCGCGCGCCCGAGCTGCTGCTCAACTCCACCGACTACTCGGCGGCCATCGACGTCTGGTCCGTCGGCTGCATCTTCATGGAGCTCATCGACCGCCGCCCGCTCTTCCCCGGCCGCGACCACATGCACCAGATGCGCCTCATAACCGAGGTGATTGGAACGCCGACGGACGACGAGCTCGGCTTCACCCGGAACGAGGACGCGCGGAAGTACATGCGCCACCTCCCGCAATTCCCGCGCCGGCCGTTCGCGAGCCTGTTCCCCCGGGTGCAGCCCCTCGCGCTGGACCTCATCGAGCGCATGCTCACCTTCAACCCGCTGCAGAGGATCACAGGTTTTGTCCTCGCACTTCTCACAGTCACCGGGATCTGTTCTGCCATGCAGTTGCGGAGGCGCTGGCGCACCCGTATCTGGAGCGGCTACACGACGTCGACGACGAGCCCGTCTGCACGGAGCCGTTCTCGTTCGACTTCGAGCGGCAGGCTCTGACAGAAGACCAGATGAAGCAGCTGATATTCAACGAGGCCATCGAGCTGAACCCCAGTTTCCGATATTAGAGATGGATCGGATCCGTGCACGATTCCTCGTTAATGCACGGTGTTTGTAAATAGGCGTAATATAAGATTGAAGGCTGCATTTGTTTGTTTGTTTGTTTTGATGTTCGAACCAAGGCAACCATCCTGTGTCTGTGAGGCCGTGTAATCTGTCGCTGCAGGTTTCAGGCGTCCCTGTAATGACTAATGAGTTGTACCAAACATAAAAGGTTCGGATCAAAAGGCGATGAGTTGTACCAACTACCAAGATAAAAAAGGTGATGTGATGGTTAATGGTATCATTTTCAAGTTATATGAGAATTTAGTGATAATTTGTATTCGTGGGCCCTCTTTACGTATATCTTAATCCAAGCAAACATGTATTTAGTCCTTAG - >Zm00001eb013100_T003
GTCCTCCCATCAGCGCCGTCCGTAGCGGAGCAGTGCCAGTGGCCGTGCCGTGCGTCACGCACAAGGCACACGGCCCCCCGCCCGGTCGTGGGCAGAGCACCCGGCGGCAGCGATCGAGGCTTTGCTCCTGCTTCGGCTTCAGCCCCCCCAATTCTTTATAAAAATCCCTCGCCAGCGCTTCCCTCTTTTGACAAATCGGCCGCGGGGGAGAGAGATATATAGAGCCAATTTTTGCCCGATTTGCCTGTCTGCTCGGGACTGAGCCGGTATTCCTCTTCCTCGTCGACGAGAATAAATAAGCTAAGCCAGGGCCCCGGGGAAGAAGAAGATGAGCTCCGCCGGAGGCATGGACGGGTCTCCGCCGGTCGCCGAATTCCAGCCGACGGTGACGCACGGCGGCCGGTTCCTCCGGTACAATATCTTCGGCAACCTGTTCGAGATCACGCGCAAGTACCAGCCTCCCGTCATGCCCATCGGCCGCGGCGCCTACGGGATCGTCTGCTCGGTGATGAACTTCGAGACGAGGGAGATGGTGGCCATCAAGAAGATCGCCAACGCCTTCGACAACCACATGGACGCCAAGCGCACGCTCCGGGAGATCAAGCTGATGCGGCACCTCGACCACGAGAACATCATCGGCATCAGGGACGTGATCCCGCCGCCGATCCCGCGGGCTTTCAACGACGTGTACATCGGCATGGAGTTCATGGAGACGGACCTCCACCAGATCATCCGCTCCGGGCAGGAGCTGTCGGAGGAGCACTGCCAGTACTTCCTGTACCAGATCCTGCGCGGGCTCAAGTACATCCACTCCGCCGGCGTGATCCACCGCGACCTCAAGCCCAGCAACCTGCTGCTCAGCGCCAACTGCGACCTCAAGATCTGCGACTTCGGGCTGGCGCGCCCGTCCTCCGACAGCGACATGATGACGGAGTACGTGGTGACGCGGTGGTACCGCGCGCCCGAGCTGCTGCTCAACTCCACCGACTACTCGGCGGCCATCGACGTCTGGTCCGTCGGCTGCATCTTCATGGAGCTCATCGACCGCCGCCCGCTCTTCCCCGGCCGCGACCACATGCACCAGATGCGCCTCATAACCGAGGTGATTGGAACGCCGACGGACGACGAGCTCGGCTTCACCCGGAACGAGGACGCGCGGAAGTACATGCGCCACCTCCCGCAATTCCCGCGCCGGCCGTTCGCGAGCCTGTTCCCCCGGGTGCAGCCCCTCGCGCTGGACCTCATCGAGCGCATGCTCACCTTCAACCCGCTGCAGAGGATCACAGGTTTTGTCCTCGCACTTCTCACAGTCACCGGGATCTGTTCTGCCATGCAGTTGCGGAGGCGCTGGCGCACCCGTATCTGGAGCGGCTACACGACGTCGACGACGAGCCCGTCTGCACGGAGCCGTTCTCGTTCGACTTCGAGCGGCAGGCTCTGACAGAAGACCAGATGAAGCAGCTGATATTCAACGAGGCCATCGAGCTGAACCCCAGTTTCCGATATTAGAGATGGATCGGATCCGTGCACGATTCCTCGTTAATGCACGGTGTTTGTAAATAGGCGTAATATAAGATTGAAGGCTGCATTTGTTTGTTTGTTTGTTTTGATGTTCGAACCAAGGCAACCATCCTGTGTCTGTGAGGCCGTGTAATCTGTCGCTGCAGGTTTCAGGCGTCCCTGTAATGACTAATGAGTTGTACCAAACATAAAAGGTTCGGATCAAAAGGCGATGAGTTGTACCAACTACCAAGATAAAAAAGGTGATGTGATGGTTAATGGTATCATTTTCAAGTTATATGAGAATTTAGTGATAATTTGTATTCGTGGGCCCTCTTTACGTATATCTTAATCCAAGCAAACATGTATTTAGTCCTTAG - >Zm00001eb013100_T002
GTCCTCCCATCAGCGCCGTCCGTAGCGGAGCAGTGCCAGTGGCCGTGCCGTGCGTCACGCACAAGGCACACGGCCCCCCGCCCGGTCGTGGGCAGAGCACCCGGCGGCAGCGATCGAGGCTTTGCTCCTGCTTCGGCTTCAGCCCCCCCAATTCTTTATAAAAATCCCTCGCCAGCGCTTCCCTCTTTTGACAAATCGGCCGCGGGGGAGAGAGATATATAGAGCCAATTTTTGCCCGATTTGCCTGTCTGCTCGGGACTGAGCCGGTATTCCTCTTCCTCGTCGACGAGAATAAATAAGCTAAGCCAGGGCCCCGGGGAAGAAGAAGATGAGCTCCGCCGGAGGCATGGACGGGTCTCCGCCGGTCGCCGAATTCCAGCCGACGGTGACGCACGGCGGCCGGTTCCTCCGGTACAATATCTTCGGCAACCTGTTCGAGATCACGCGCAAGTACCAGCCTCCCGTCATGCCCATCGGCCGCGGCGCCTACGGGATCGTCTGCTCGGTGATGAACTTCGAGACGAGGGAGATGGTGGCCATCAAGAAGATCGCCAACGCCTTCGACAACCACATGGACGCCAAGCGCACGCTCCGGGAGATCAAGCTGATGCGGCACCTCGACCACGAGAACATCATCGGCATCAGGGACGTGATCCCGCCGCCGATCCCGCGGGCTTTCAACGACGTGTACATCGGCATGGAGTTCATGGAGACGGACCTCCACCAGATCATCCGCTCCGGGCAGGAGCTGTCGGAGGAGCACTGCCAGTACTTCCTGTACCAGATCCTGCGCGGGCTCAAGTACATCCACTCCGCCGGCGTGATCCACCGCGACCTCAAGCCCAGCAACCTGCTGCTCAGCGCCAACTGCGACCTCAAGATCTGCGACTTCGGGCTGGCGCGCCCGTCCTCCGACAGCGACATGATGACGGAGTACGTGGTGACGCGGTGGTACCGCGCGCCCGAGCTGCTGCTCAACTCCACCGACTACTCGGCGGCCATCGACGTCTGGTCCGTCGGCTGCATCTTCATGGAGCTCATCGACCGCCGCCCGCTCTTCCCCGGCCGCGACCACATGCACCAGATGCGCCTCATAACCGAGGTGATTGGAACGCCGACGGACGACGAGCTCGGCTTCACCCGGAACGAGGACGCGCGGAAGTACATGCGCCACCTCCCGCAATTCCCGCGCCGGCCGTTCGCGAGCCTGTTCCCCCGGGTGCAGCCCCTCGCGCTGGACCTCATCGAGCGCATGCTCACCTTCAACCCGCTGCAGAGGATCACAGGTTTTGTCCTCGCACTTCTCACAGTCACCGGGATCTGTTCTGCCATGCAGTTGCGGAGGCGCTGGCGCACCCGTATCTGGAGCGGCTACACGACGTCGACGACGAGCCCGTCTGCACGGAGCCGTTCTCGTTCGACTTCGAGCGGCAGGCTCTGACAGAAGACCAGATGAAGCAGCTGATATTCAACGAGGCCATCGAGCTGAACCCCAGTTTCCGATATTAGAGATGGATCGGATCCGTGCACGATTCCTCGTTAATGCACGGTGTTTGTAAATAGGCGTAATATAAGATTGAAGGCTGCATTTGTTTGTTTGTTTGTTTTGATGTTCGAACCAAGGCAACCATCCTGTGTCTGTGAGGCCGTGTAATCTGTCGCTGCAGGTTTCAGGCGTCCCTGTAATGACTAATGAGTTGTACCAAACATAAAAGGTTCGGATCAAAAGGCGATGAGTTGTACCAACTACCAAGATAAAAAAGGTGATGTGATGGTTAATGGTATCATTTTCAAGTTATATGAGAATTTAGTGATAATTTGTATTCGTGGGCCCTCTTTACGTATATCTTAATCCAAGCAAACATGTATTTAGTCCTTAG
CDS Sequence
- >Zm00001eb013100_T001
ATGAGCTCCGCCGGAGGCATGGACGGGTCTCCGCCGGTCGCCGAATTCCAGCCGACGGTGACGCACGGCGGCCGGTTCCTCCGGTACAATATCTTCGGCAACCTGTTCGAGATCACGCGCAAGTACCAGCCTCCCGTCATGCCCATCGGCCGCGGCGCCTACGGGATCGTCTGCTCGGTGATGAACTTCGAGACGAGGGAGATGGTGGCCATCAAGAAGATCGCCAACGCCTTCGACAACCACATGGACGCCAAGCGCACGCTCCGGGAGATCAAGCTGATGCGGCACCTCGACCACGAGAACATCATCGGCATCAGGGACGTGATCCCGCCGCCGATCCCGCGGGCTTTCAACGACGTGTACATCGGCATGGAGTTCATGGAGACGGACCTCCACCAGATCATCCGCTCCGGGCAGGAGCTGTCGGAGGAGCACTGCCAGTACTTCCTGTACCAGATCCTGCGCGGGCTCAAGTACATCCACTCCGCCGGCGTGATCCACCGCGACCTCAAGCCCAGCAACCTGCTGCTCAGCGCCAACTGCGACCTCAAGATCTGCGACTTCGGGCTGGCGCGCCCGTCCTCCGACAGCGACATGATGACGGAGTACGTGGTGACGCGGTGGTACCGCGCGCCCGAGCTGCTGCTCAACTCCACCGACTACTCGGCGGCCATCGACGTCTGGTCCGTCGGCTGCATCTTCATGGAGCTCATCGACCGCCGCCCGCTCTTCCCCGGCCGCGACCACATGCACCAGATGCGCCTCATAACCGAGGTGATTGGAACGCCGACGGACGACGAGCTCGGCTTCACCCGGAACGAGGACGCGCGGAAGTACATGCGCCACCTCCCGCAATTCCCGCGCCGGCCGTTCGCGAGCCTGTTCCCCCGGGTGCAGCCCCTCGCGCTGGACCTCATCGAGCGCATGCTCACCTTCAACCCGCTGCAGAGGATCACAGGTTTTGTCCTCGCACTTCTCACAGTCACCGGGATCTGTTCTGCCATGCAGTTGCGGAGGCGCTGGCGCACCCGTATCTGGAGCGGCTACACGACGTCGACGACGAGCCCGTCTGCACGGAGCCGTTCTCGTTCGACTTCGAGCGGCAGGCTCTGA - >Zm00001eb013100_T003
ATGAGCTCCGCCGGAGGCATGGACGGGTCTCCGCCGGTCGCCGAATTCCAGCCGACGGTGACGCACGGCGGCCGGTTCCTCCGGTACAATATCTTCGGCAACCTGTTCGAGATCACGCGCAAGTACCAGCCTCCCGTCATGCCCATCGGCCGCGGCGCCTACGGGATCGTCTGCTCGGTGATGAACTTCGAGACGAGGGAGATGGTGGCCATCAAGAAGATCGCCAACGCCTTCGACAACCACATGGACGCCAAGCGCACGCTCCGGGAGATCAAGCTGATGCGGCACCTCGACCACGAGAACATCATCGGCATCAGGGACGTGATCCCGCCGCCGATCCCGCGGGCTTTCAACGACGTGTACATCGGCATGGAGTTCATGGAGACGGACCTCCACCAGATCATCCGCTCCGGGCAGGAGCTGTCGGAGGAGCACTGCCAGTACTTCCTGTACCAGATCCTGCGCGGGCTCAAGTACATCCACTCCGCCGGCGTGATCCACCGCGACCTCAAGCCCAGCAACCTGCTGCTCAGCGCCAACTGCGACCTCAAGATCTGCGACTTCGGGCTGGCGCGCCCGTCCTCCGACAGCGACATGATGACGGAGTACGTGGTGACGCGGTGGTACCGCGCGCCCGAGCTGCTGCTCAACTCCACCGACTACTCGGCGGCCATCGACGTCTGGTCCGTCGGCTGCATCTTCATGGAGCTCATCGACCGCCGCCCGCTCTTCCCCGGCCGCGACCACATGCACCAGATGCGCCTCATAACCGAGGTGATTGGAACGCCGACGGACGACGAGCTCGGCTTCACCCGGAACGAGGACGCGCGGAAGTACATGCGCCACCTCCCGCAATTCCCGCGCCGGCCGTTCGCGAGCCTGTTCCCCCGGGTGCAGCCCCTCGCGCTGGACCTCATCGAGCGCATGCTCACCTTCAACCCGCTGCAGAGGATCACAGGTTTTGTCCTCGCACTTCTCACAGTCACCGGGATCTGTTCTGCCATGCAGTTGCGGAGGCGCTGGCGCACCCGTATCTGGAGCGGCTACACGACGTCGACGACGAGCCCGTCTGCACGGAGCCGTTCTCGTTCGACTTCGAGCGGCAGGCTCTGA - >Zm00001eb013100_T002
ATGAGCTCCGCCGGAGGCATGGACGGGTCTCCGCCGGTCGCCGAATTCCAGCCGACGGTGACGCACGGCGGCCGGTTCCTCCGGTACAATATCTTCGGCAACCTGTTCGAGATCACGCGCAAGTACCAGCCTCCCGTCATGCCCATCGGCCGCGGCGCCTACGGGATCGTCTGCTCGGTGATGAACTTCGAGACGAGGGAGATGGTGGCCATCAAGAAGATCGCCAACGCCTTCGACAACCACATGGACGCCAAGCGCACGCTCCGGGAGATCAAGCTGATGCGGCACCTCGACCACGAGAACATCATCGGCATCAGGGACGTGATCCCGCCGCCGATCCCGCGGGCTTTCAACGACGTGTACATCGGCATGGAGTTCATGGAGACGGACCTCCACCAGATCATCCGCTCCGGGCAGGAGCTGTCGGAGGAGCACTGCCAGTACTTCCTGTACCAGATCCTGCGCGGGCTCAAGTACATCCACTCCGCCGGCGTGATCCACCGCGACCTCAAGCCCAGCAACCTGCTGCTCAGCGCCAACTGCGACCTCAAGATCTGCGACTTCGGGCTGGCGCGCCCGTCCTCCGACAGCGACATGATGACGGAGTACGTGGTGACGCGGTGGTACCGCGCGCCCGAGCTGCTGCTCAACTCCACCGACTACTCGGCGGCCATCGACGTCTGGTCCGTCGGCTGCATCTTCATGGAGCTCATCGACCGCCGCCCGCTCTTCCCCGGCCGCGACCACATGCACCAGATGCGCCTCATAACCGAGGTGATTGGAACGCCGACGGACGACGAGCTCGGCTTCACCCGGAACGAGGACGCGCGGAAGTACATGCGCCACCTCCCGCAATTCCCGCGCCGGCCGTTCGCGAGCCTGTTCCCCCGGGTGCAGCCCCTCGCGCTGGACCTCATCGAGCGCATGCTCACCTTCAACCCGCTGCAGAGGATCACAGGTTTTGTCCTCGCACTTCTCACAGTCACCGGGATCTGTTCTGCCATGCAGTTGCGGAGGCGCTGGCGCACCCGTATCTGGAGCGGCTACACGACGTCGACGACGAGCCCGTCTGCACGGAGCCGTTCTCGTTCGACTTCGAGCGGCAGGCTCTGA
Protein Sequence
- >Zm00001eb013100_P001
MSSAGGMDGSPPVAEFQPTVTHGGRFLRYNIFGNLFEITRKYQPPVMPIGRGAYGIVCSVMNFETREMVAIKKIANAFDNHMDAKRTLREIKLMRHLDHENIIGIRDVIPPPIPRAFNDVYIGMEFMETDLHQIIRSGQELSEEHCQYFLYQILRGLKYIHSAGVIHRDLKPSNLLLSANCDLKICDFGLARPSSDSDMMTEYVVTRWYRAPELLLNSTDYSAAIDVWSVGCIFMELIDRRPLFPGRDHMHQMRLITEVIGTPTDDELGFTRNEDARKYMRHLPQFPRRPFASLFPRVQPLALDLIERMLTFNPLQRITGFVLALLTVTGICSAMQLRRRWRTRIWSGYTTSTTSPSARSRSRSTSSGRL - >Zm00001eb013100_P003
MSSAGGMDGSPPVAEFQPTVTHGGRFLRYNIFGNLFEITRKYQPPVMPIGRGAYGIVCSVMNFETREMVAIKKIANAFDNHMDAKRTLREIKLMRHLDHENIIGIRDVIPPPIPRAFNDVYIGMEFMETDLHQIIRSGQELSEEHCQYFLYQILRGLKYIHSAGVIHRDLKPSNLLLSANCDLKICDFGLARPSSDSDMMTEYVVTRWYRAPELLLNSTDYSAAIDVWSVGCIFMELIDRRPLFPGRDHMHQMRLITEVIGTPTDDELGFTRNEDARKYMRHLPQFPRRPFASLFPRVQPLALDLIERMLTFNPLQRITGFVLALLTVTGICSAMQLRRRWRTRIWSGYTTSTTSPSARSRSRSTSSGRL - >Zm00001eb013100_P002
MSSAGGMDGSPPVAEFQPTVTHGGRFLRYNIFGNLFEITRKYQPPVMPIGRGAYGIVCSVMNFETREMVAIKKIANAFDNHMDAKRTLREIKLMRHLDHENIIGIRDVIPPPIPRAFNDVYIGMEFMETDLHQIIRSGQELSEEHCQYFLYQILRGLKYIHSAGVIHRDLKPSNLLLSANCDLKICDFGLARPSSDSDMMTEYVVTRWYRAPELLLNSTDYSAAIDVWSVGCIFMELIDRRPLFPGRDHMHQMRLITEVIGTPTDDELGFTRNEDARKYMRHLPQFPRRPFASLFPRVQPLALDLIERMLTFNPLQRITGFVLALLTVTGICSAMQLRRRWRTRIWSGYTTSTTSPSARSRSRSTSSGRL