Gene Details:

Functional Descriptions:

  • Comprehensive genetic and molecular studies reveal the roles and mechanisms of two hub genes,ZmBx12 and ZmGLK44, in regulating maize metabolite biosynthesis and drought tolerance.
  • ZmBx12 and ZmGLK44, for their role in regulating benzoxazinoids and tryptophan biosynthesis as well as maize drought tolerance via comprehensive genetic and molecular methods.
  • We further verified two hub candidate genes,ZmBx12 and ZmGLK44, for their role in regulating benzoxazinoids and tryptophan biosynthesis as well as maize drought tolerance via comprehensive genetic and molecular methods. Our results provide novel insights into the genetic bases of metabolome-mediated drought adaptation in a crop.
  • Based on our data sets, ZmGLK44 is a hub gene correlated / associated with 30 drought-responsive metabolites. Eight of these are annotated amino acids. To elucidate the roles of ZmGLK44 in regulating the biosynthesis of these drought-responsive metabolites, we generated ZmGLK44 inducible overexpression transgene maize lines.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Zm00001eb058460_T002
    CGCGAGGGGTGCAGGCGCAAAACTACGCGTGTGGTGTGAGAAGCGGTAACTCGAGGCCGCGCCGATGGAGGGATCCCACGATTCACCGTGGAGTGTTGAGAGAGACAGAGACGTCCGGCTCCGGCCGCGACCGCGAGCTTATCGCGCTGGATGCGACGTGAGGAATCGGCAACGCAGCGCGCGCCCACCGCACCCCAGGCGCCGCGTGGTTTGGGGGCGTGGTCTCTCTCTCTCTCTCTCCTCCCATCCTGCGGTCCAGCTCGCAGATTCCATTCGCCCCGCTCGCCGCCACCACCCGTCTATACATATGCCAGGGCGGGCGCACCTCCGGAATCCAGATCCCCTCACAGTCATCCTCCACCCACCCCACCCACCGCCTCCGCCTCTCTCTCTTTCGTTTGTTCGTCTCGGCTTCCGGTCGCCGATTCTTCTCTGCGCAAAAGATTCCTGGTGGTGGTGCCCGTACCTTGCTCCACGCCTCGCTCGCGTCGCGTGCATGGGGCTGGACGTCGGCGGCATCGGGATGGGCCTGGATCTGGGTCTGGACCTCGGGCTGTTCGCCGCGCGGAGCGCCGGAGGGATGGCGGCCGCGGCCAAGGGTGCGCCGGCGGAGATCGAGTCCTGCATCAGGAGCCTCGAGGAGGAGCGCCGGAAGATCGAGGTGTTCAGGCGCGAGCTCCCGCTCTGCGTGCGTCTCCTCGCCGATGTGATCGACGAATTGAAGGATGAGGCCGCCAAGAGAGGCGGAGACGCGGAGGCCAAGGCCGACGACGGCGATAAGCGGAAATGGATGAGCACCGCTCAGCTGTGGCTCGACAGCGACGCCAAATCCGACGAGTCTGACAAAGAGCAACTGAGTGAAATCACTTCGCCGGAGCCCAAGTTGCTCGGTGGCGCACCCATGCCCATAAGGGCCGTTGCGGCGGTGCCACCCCTGCCGCCTCCATTCTTCAGGAGAGAAGACAGCTCTGCCGGCAGTGGTCTGTCGCTGGTACCACCCGCAGCTAAGCCGCCGATTCCTCCCATGTCTGCCAGCGACAACGCGAGCGGCAGATTCTGTGCCACTATGCCACCGTCTGGCTCTGGGGCCAACTTGCACTCTCAGGCTCAGCAGCAAGCCAGGAAGGCGAGGCGGTGCTGGTCGCCGGAGCTTCACCGGCTGTTTGTTGCCGCCTTGCATCAACTCGGCGGCCCTCAAGTTGCTACTCCGAAGCAGATCAGGGAGGTGATGAAGGTGGACGGGCTTACAAACGATGAAGTGAAAAGCCATCTCCAGAAGTACCGGCTGCACAACAGAAGATCCCCCGGCGTCGTGGCTCCGGTGAGCCAGTCGGTCATGCTGGCGGGCGGCCTCTGGGCTCCTCCTCATCAGGAGCAAAGCAGCTCGCAGTCCGGGTCTCCCCAGGGCCCGCTTCAGTTCTCTGGATCCGGTGTAGCGGCCACCGTCGGCGGCGATAGCAGCAGCAGCGACGAGGACGACAAGTCCGAAGGCTACAGCCGGAAATACGTGTAAAGGCCGCGTTCGACGGATGAGAGTGTAGAGATAGCGTCGCGGCTTCTGCTTGCCCGTCTGTGGCAATAACAGATTCATCAGTCTTGAGAGAGGTTGTAGTGCGTGCGCCCAAGAGCTCAACTGATCATGGGTTGGATTGTTTGTTTGTCATGTACGTACATGGAAAGCTGATGGCTTCCTGACTTTTAATTTCTGGGCAGGTCCCAAAATTTTCGCCCTGAGAATGAGATATTATATAGATAACATACATACATACCATATTCGTTGCCCGCCTGTTGTCTTGAATCGCGAATGGTTGCAGCGTTGTTGAATTGAATGAAATAAATACATGCCCTTGTCAGCGTCCCTG
  • >Zm00001eb058460_T001
    CGCGAGGGGTGCAGGCGCAAAACTACGCGTGTGGTGTGAGAAGCGGTAACTCGAGGCCGCGCCGATGGAGGGATCCCACGATTCACCGTGGAGTGTTGAGAGAGACAGAGACGTCCGGCTCCGGCCGCGACCGCGAGCTTATCGCGCTGGATGCGACGTGAGGAATCGGCAACGCAGCGCGCGCCCACCGCACCCCAGGCGCCGCGTGGTTTGGGGGCGTGGTCTCTCTCTCTCTCTCTCCTCCCATCCTGCGGTCCAGCTCGCAGATTCCATTCGCCCCGCTCGCCGCCACCACCCGTCTATACATATGCCAGGGCGGGCGCACCTCCGGAATCCAGATCCCCTCACAGTCATCCTCCACCCACCCCACCCACCGCCTCCGCCTCTCTCTCTTTCGTTTGTTCGTCTCGGCTTCCGGTCGCCGATTCTTCTCTGCGCAAAAGATTCCTGGTGGTGGTGCCCGTACCTTGCTCCACGCCTCGCTCGCGTCGCGTGCATGGGGCTGGACGTCGGCGGCATCGGGATGGGCCTGGATCTGGGTCTGGACCTCGGGCTGTTCGCCGCGCGGAGCGCCGGAGGGATGGCGGCCGCGGCCAAGGGTGCGCCGGCGGAGATCGAGTCCTGCATCAGGAGCCTCGAGGAGGAGCGCCGGAAGATCGAGGTGTTCAGGCGCGAGCTCCCGCTCTGCGTGCGTCTCCTCGCCGATGTGATCGACGAATTGAAGGATGAGGCCGCCAAGAGAGGCGGAGACGCGGAGGCCAAGGCCGACGACGGCGATAAGCGGAAATGGATGAGCACCGCTCAGCTGTGGCTCGACAGCGACGCCAAATCCGACGAGTCTGACAAAGAGCAACTGAGTGAAATCACTTCGCCGGAGCCCAAGTTGCTCGGTGGCGCACCCATGCCCATAAGGGCCGTTGCGGCGGTGCCACCCCTGCCGCCTCCATTCTTCAGGAGAGAAGACAGCTCTGCCGGCAGTGGTCTGTCGCTGGTACCACCCGCAGCTAAGCCGCCGATTCCTCCCATGTCTGCCAGCGACAACGCGAGCGGCAGATTCTGTGCCACTATGCCACCGTCTGGCTCTGGGGCCAACTTGCACTCTCAGGCTCAGCAGCAAGCCAGGAAGGCGAGGCGGTGCTGGTCGCCGGAGCTTCACCGGCTGTTTGTTGCCGCCTTGCATCAACTCGGCGGCCCTCAAGTTGCTACTCCGAAGCAGATCAGGGAGGTGATGAAGGTGGACGGGCTTACAAACGATGAAGTGAAAAGCCATCTCCAGAAGTACCGGCTGCACAACAGAAGATCCCCCGGCGTCGTGGCTCCGGTGAGCCAGTCGGTCATGCTGGCGGGCGGCCTCTGGGCTCCTCCTCATCAGGAGCAAAGCAGCTCGCAGTCCGGGTCTCCCCAGGGCCCGCTTCAGTTCTCTGGATCCGGTGTAGCGGCCACCGTCGGCGGCGATAGCAGCAGCAGCGACGAGGACGACAAGTCCGAAGGCTACAGCCGGAAATACGTGTAAAGGCCGCGTTCGACGGATGAGAGTGTAGAGATAGCGTCGCGGCTTCTGCTTGCCCGTCTGTGGCAATAACAGATTCATCAGTCTTGAGAGAGGTTGTAGTGCGTGCGCCCAAGAGCTCAACTGATCATGGGTTGGATTGTTTGTTTGTCATGTACGTACATGGAAAGCTGATGGCTTCCTGACTTTTAATTTCTGGGCAGGTCCCAAAATTTTCGCCCTGAGAATGAGATATTATATAGATAACATACATACATACCATATTCGTTGCCCGCCTGTTGTCTTGAATCGCGAATGGTTGCAGCGTTGTTGAATTGAATGAAATAAATACATGCCCTTGTCAGCGTCCCTG
CDS Sequence
  • >Zm00001eb058460_T002
    ATGGGGCTGGACGTCGGCGGCATCGGGATGGGCCTGGATCTGGGTCTGGACCTCGGGCTGTTCGCCGCGCGGAGCGCCGGAGGGATGGCGGCCGCGGCCAAGGGTGCGCCGGCGGAGATCGAGTCCTGCATCAGGAGCCTCGAGGAGGAGCGCCGGAAGATCGAGGTGTTCAGGCGCGAGCTCCCGCTCTGCGTGCGTCTCCTCGCCGATGTGATCGACGAATTGAAGGATGAGGCCGCCAAGAGAGGCGGAGACGCGGAGGCCAAGGCCGACGACGGCGATAAGCGGAAATGGATGAGCACCGCTCAGCTGTGGCTCGACAGCGACGCCAAATCCGACGAGTCTGACAAAGAGCAACTGAGTGAAATCACTTCGCCGGAGCCCAAGTTGCTCGGTGGCGCACCCATGCCCATAAGGGCCGTTGCGGCGGTGCCACCCCTGCCGCCTCCATTCTTCAGGAGAGAAGACAGCTCTGCCGGCAGTGGTCTGTCGCTGGTACCACCCGCAGCTAAGCCGCCGATTCCTCCCATGTCTGCCAGCGACAACGCGAGCGGCAGATTCTGTGCCACTATGCCACCGTCTGGCTCTGGGGCCAACTTGCACTCTCAGGCTCAGCAGCAAGCCAGGAAGGCGAGGCGGTGCTGGTCGCCGGAGCTTCACCGGCTGTTTGTTGCCGCCTTGCATCAACTCGGCGGCCCTCAAGTTGCTACTCCGAAGCAGATCAGGGAGGTGATGAAGGTGGACGGGCTTACAAACGATGAAGTGAAAAGCCATCTCCAGAAGTACCGGCTGCACAACAGAAGATCCCCCGGCGTCGTGGCTCCGGTGAGCCAGTCGGTCATGCTGGCGGGCGGCCTCTGGGCTCCTCCTCATCAGGAGCAAAGCAGCTCGCAGTCCGGGTCTCCCCAGGGCCCGCTTCAGTTCTCTGGATCCGGTGTAGCGGCCACCGTCGGCGGCGATAGCAGCAGCAGCGACGAGGACGACAAGTCCGAAGGCTACAGCCGGAAATACGTGTAA
  • >Zm00001eb058460_T001
    ATGGGGCTGGACGTCGGCGGCATCGGGATGGGCCTGGATCTGGGTCTGGACCTCGGGCTGTTCGCCGCGCGGAGCGCCGGAGGGATGGCGGCCGCGGCCAAGGGTGCGCCGGCGGAGATCGAGTCCTGCATCAGGAGCCTCGAGGAGGAGCGCCGGAAGATCGAGGTGTTCAGGCGCGAGCTCCCGCTCTGCGTGCGTCTCCTCGCCGATGTGATCGACGAATTGAAGGATGAGGCCGCCAAGAGAGGCGGAGACGCGGAGGCCAAGGCCGACGACGGCGATAAGCGGAAATGGATGAGCACCGCTCAGCTGTGGCTCGACAGCGACGCCAAATCCGACGAGTCTGACAAAGAGCAACTGAGTGAAATCACTTCGCCGGAGCCCAAGTTGCTCGGTGGCGCACCCATGCCCATAAGGGCCGTTGCGGCGGTGCCACCCCTGCCGCCTCCATTCTTCAGGAGAGAAGACAGCTCTGCCGGCAGTGGTCTGTCGCTGGTACCACCCGCAGCTAAGCCGCCGATTCCTCCCATGTCTGCCAGCGACAACGCGAGCGGCAGATTCTGTGCCACTATGCCACCGTCTGGCTCTGGGGCCAACTTGCACTCTCAGGCTCAGCAGCAAGCCAGGAAGGCGAGGCGGTGCTGGTCGCCGGAGCTTCACCGGCTGTTTGTTGCCGCCTTGCATCAACTCGGCGGCCCTCAAGTTGCTACTCCGAAGCAGATCAGGGAGGTGATGAAGGTGGACGGGCTTACAAACGATGAAGTGAAAAGCCATCTCCAGAAGTACCGGCTGCACAACAGAAGATCCCCCGGCGTCGTGGCTCCGGTGAGCCAGTCGGTCATGCTGGCGGGCGGCCTCTGGGCTCCTCCTCATCAGGAGCAAAGCAGCTCGCAGTCCGGGTCTCCCCAGGGCCCGCTTCAGTTCTCTGGATCCGGTGTAGCGGCCACCGTCGGCGGCGATAGCAGCAGCAGCGACGAGGACGACAAGTCCGAAGGCTACAGCCGGAAATACGTGTAA
Protein Sequence
  • >Zm00001eb058460_P002
    MGLDVGGIGMGLDLGLDLGLFAARSAGGMAAAAKGAPAEIESCIRSLEEERRKIEVFRRELPLCVRLLADVIDELKDEAAKRGGDAEAKADDGDKRKWMSTAQLWLDSDAKSDESDKEQLSEITSPEPKLLGGAPMPIRAVAAVPPLPPPFFRREDSSAGSGLSLVPPAAKPPIPPMSASDNASGRFCATMPPSGSGANLHSQAQQQARKARRCWSPELHRLFVAALHQLGGPQVATPKQIREVMKVDGLTNDEVKSHLQKYRLHNRRSPGVVAPVSQSVMLAGGLWAPPHQEQSSSQSGSPQGPLQFSGSGVAATVGGDSSSSDEDDKSEGYSRKYV
  • >Zm00001eb058460_P001
    MGLDVGGIGMGLDLGLDLGLFAARSAGGMAAAAKGAPAEIESCIRSLEEERRKIEVFRRELPLCVRLLADVIDELKDEAAKRGGDAEAKADDGDKRKWMSTAQLWLDSDAKSDESDKEQLSEITSPEPKLLGGAPMPIRAVAAVPPLPPPFFRREDSSAGSGLSLVPPAAKPPIPPMSASDNASGRFCATMPPSGSGANLHSQAQQQARKARRCWSPELHRLFVAALHQLGGPQVATPKQIREVMKVDGLTNDEVKSHLQKYRLHNRRSPGVVAPVSQSVMLAGGLWAPPHQEQSSSQSGSPQGPLQFSGSGVAATVGGDSSSSDEDDKSEGYSRKYV

Source: Genetic information comes from the article