Gene Details:

Functional Descriptions:

  • To accomplish this, we utilized a dominant, miRNA-based construct expressed in a hemizygous state to selectively reduce expression of the ZmGA20ox3 and Zmga20ox5 genes that control GA biosynthesis primarily in vegetative tissues.
  • ZmGA20ox3 and Zmga20ox5 genes that control GA biosynthesis primarily in vegetative tissues.
  • Targeted suppression of gibberellin biosynthetic genes ZmGA20ox3 and Zmga20ox5 produces a short stature maize ideotype.
  • These factors identified ZmGA20ox3 and Zmga20ox5 as the most likely candidates for gene suppression to reduce GA levels in maize stems without impacting reproductive tissues.
  • Suppression of ZmGA20ox3 and Zmga20ox5 reduces the levels of bioactive GA.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Zm00001eb366090_T002
    GTCGGGTCCCACATGAGGCCGCGCCTCCCTCCAAATGTTCCCTCCCTGCCTTCGTCTTTGTCGTTGCTCGCAAACTCCCTGTCCTCCCCTGTTACAAATACCCCCACCCGCCCGGACAGCTTCCCTGCATACTTGCAGCTCGCACATCTCATGGTGTCGCAGGAACGACAAGAGCCAGCTGTGCCTAGCAGCAGCAGCAGCAGCGCCAAGCGCGCAGCCACGTCCATGGACGCCAGCCCGGCCCCGCCGCTCCTCCTCCGCGCCCCCACTCCCAGCCCCAGCATTGACCTCCCCGCTGGCAAGGACAAGGCCGACGCGGCGGCCAGCAAGGCCGGCGCGGCCGTGTTCGACCTGCGCCGGGAGCCCAAGATCCCCGCGCCATTCCTGTGGCCGCAGGAAGAGGCGCGGCCGTCCTCGGCCGCGGAGCTGGAGGTGCCGATGGTGGACGTGGGCGTGCTGCGCAATGGCGACCGCGCGGGGCTGCGGCGCGCCGCGGCGCAGGTGGCCGCGGCGTGCGCGACGCACGGGTTCTTCCAGGTGTGCGGGCACGGCGTGGACGCGGCGCTGGGGCGCGCCGCGCTGGACGGCGCCAGCGACTTCTTCCGGCTGCCGCTCGCCGAGAAGCAGCGCGCCCGGCGCGTCCCCGGCACCGTGTCCGGGTACACGAGCGCGCACGCCGACCGGTTCGCGGCCAAGCTCCCCTGGAAGGAGACCCTGTCGTTCGGCTACCACGACGGCGCCGCGTCGCCTGTCGTCGTGGACTACTTCGTCGGCACCCTCGGCCAGGATTTCGAGCCAATGGGGTGGGTGTACCAGAGGTACTGCGAGGAGATGAAGGAGCTGTCGCTGACGATCATGGAGCTGCTGGAGCTGAGCCTGGGCGTGGAGCTGCGCGGCTACTACCGGGAGTTCTTCGAGGACAGCCGGTCCATCATGCGGTGCAACTACTACCCGCCGTGCCCGGAGCCGGAGCGCACGCTGGGCACGGGCCCGCACTGCGACCCCACGGCGCTCACCATCCTCCTGCAGGACGACGTGGGCGGGCTGGAGGTGCTGGTGGACGGTGAGTGGCGCCCCGTCCGGCCCGTCCCGGGCGCCATGGTCATCAACATCGGCGACACCTTCATGGTAACGAAACGAAAGCGCTCGCTCCTCTGTTTTCCTTGGCCGCTCTTGTCCTGTGTGTATATTCAGTTGAGCTCTCTCTGTGCTGTTATTTCCCGAATCCTAGTGGACCTAAACGGGCAGGTTATTACAGCACGCACACGTAGGCATGTCATGTAGCTAGTACATACATAGCGATGCCGATGCAAATGCAATAGAGACATGCGTTCGAGTTGGTTCCTATCTCGGCGGGCTACGGCAGGTACAC
  • >Zm00001eb366090_T003
    GTCGGGTCCCACATGAGGCCGCGCCTCCCTCCAAATGTTCCCTCCCTGCCTTCGTCTTTGTCGTTGCTCGCAAACTCCCTGTCCTCCCCTGTTACAAATACCCCCACCCGCCCGGACAGCTTCCCTGCATACTTGCAGCTCGCACATCTCATGGTGTCGCAGGAACGACAAGAGCCAGCTGTGCCTAGCAGCAGCAGCAGCAGCGCCAAGCGCGCAGCCACGTCCATGGACGCCAGCCCGGCCCCGCCGCTCCTCCTCCGCGCCCCCACTCCCAGCCCCAGCATTGACCTCCCCGCTGGCAAGGACAAGGCCGACGCGGCGGCCAGCAAGGCCGGCGCGGCCGTGTTCGACCTGCGCCGGGAGCCCAAGATCCCCGCGCCATTCCTGTGGCCGCAGGAAGAGGCGCGGCCGTCCTCGGCCGCGGAGCTGGAGGTGCCGATGGTGGACGTGGGCGTGCTGCGCAATGGCGACCGCGCGGGGCTGCGGCGCGCCGCGGCGCAGGTGGCCGCGGCGTGCGCGACGCACGGGTTCTTCCAGGTGTGCGGGCACGGCGTGGACGCGGCGCTGGGGCGCGCCGCGCTGGACGGCGCCAGCGACTTCTTCCGGCTGCCGCTCGCCGAGAAGCAGCGCGCCCGGCGCGTCCCCGGCACCGTGTCCGGGTACACGAGCGCGCACGCCGACCGGTTCGCGGCCAAGCTCCCCTGGAAGGAGACCCTGTCGTTCGGCTACCACGACGGCGCCGCGTCGCCTGTCGTCGTGGACTACTTCGTCGGCACCCTCGGCCAGGATTTCGAGCCAATGGGGTGGGTGTACCAGAGGTACTGCGAGGAGATGAAGGAGCTGTCGCTGACGATCATGGAGCTGCTGGAGCTGAGCCTGGGCGTGGAGCTGCGCGGCTACTACCGGGAGTTCTTCGAGGACAGCCGGTCCATCATGCGGTGCAACTACTACCCGCCGTGCCCGGAGCCGGAGCGCACGCTGGGCACGGGCCCGCACTGCGACCCCACGGCGCTCACCATCCTCCTGCAGGACGACGTGGGCGGGCTGGAGGTGCTGGTGGACGGTGAGTGGCGCCCCGTCCGGCCCGTCCCGGGCGCCATGGTCATCAACATCGGCGACACCTTCATGGTAACGAAACGAAAGCGCTCGCTCCTCTGTTTTCCTTGGCCGCTCTTGTCCTGTGTGTATATTCAGTTGAGCTCTCTCTGTGCTGTTATTTCCCGAATCCTAGTGGACCTAAACGGGCAGGTTATTACAGCACGCACACGTAGGCATGTCATGTAGCTAGTACATACATAGCGATGCCGATGCAAATGCAATAGAGACATGCGTTCGAGTTGGTTCCTATCTCGGCGGGCTACGGCAGGTACAC
  • >Zm00001eb366090_T001
    GTCGGGTCCCACATGAGGCCGCGCCTCCCTCCAAATGTTCCCTCCCTGCCTTCGTCTTTGTCGTTGCTCGCAAACTCCCTGTCCTCCCCTGTTACAAATACCCCCACCCGCCCGGACAGCTTCCCTGCATACTTGCAGCTCGCACATCTCATGGTGTCGCAGGAACGACAAGAGCCAGCTGTGCCTAGCAGCAGCAGCAGCAGCGCCAAGCGCGCAGCCACGTCCATGGACGCCAGCCCGGCCCCGCCGCTCCTCCTCCGCGCCCCCACTCCCAGCCCCAGCATTGACCTCCCCGCTGGCAAGGACAAGGCCGACGCGGCGGCCAGCAAGGCCGGCGCGGCCGTGTTCGACCTGCGCCGGGAGCCCAAGATCCCCGCGCCATTCCTGTGGCCGCAGGAAGAGGCGCGGCCGTCCTCGGCCGCGGAGCTGGAGGTGCCGATGGTGGACGTGGGCGTGCTGCGCAATGGCGACCGCGCGGGGCTGCGGCGCGCCGCGGCGCAGGTGGCCGCGGCGTGCGCGACGCACGGGTTCTTCCAGGTGTGCGGGCACGGCGTGGACGCGGCGCTGGGGCGCGCCGCGCTGGACGGCGCCAGCGACTTCTTCCGGCTGCCGCTCGCCGAGAAGCAGCGCGCCCGGCGCGTCCCCGGCACCGTGTCCGGGTACACGAGCGCGCACGCCGACCGGTTCGCGGCCAAGCTCCCCTGGAAGGAGACCCTGTCGTTCGGCTACCACGACGGCGCCGCGTCGCCTGTCGTCGTGGACTACTTCGTCGGCACCCTCGGCCAGGATTTCGAGCCAATGGGGTGGGTGTACCAGAGGTACTGCGAGGAGATGAAGGAGCTGTCGCTGACGATCATGGAGCTGCTGGAGCTGAGCCTGGGCGTGGAGCTGCGCGGCTACTACCGGGAGTTCTTCGAGGACAGCCGGTCCATCATGCGGTGCAACTACTACCCGCCGTGCCCGGAGCCGGAGCGCACGCTGGGCACGGGCCCGCACTGCGACCCCACGGCGCTCACCATCCTCCTGCAGGACGACGTGGGCGGGCTGGAGGTGCTGGTGGACGGTGAGTGGCGCCCCGTCCGGCCCGTCCCGGGCGCCATGGTCATCAACATCGGCGACACCTTCATGGTAACGAAACGAAAGCGCTCGCTCCTCTGTTTTCCTTGGCCGCTCTTGTCCTGTGTGTATATTCAGTTGAGCTCTCTCTGTGCTGTTATTTCCCGAATCCTAGTGGACCTAAACGGGCAGGTTATTACAGCACGCACACGTAGGCATGTCATGTAGCTAGTACATACATAGCGATGCCGATGCAAATGCAATAGAGACATGCGTTCGAGTTGGTTCCTATCTCGGCGGGCTACGGCAGGTACAC
CDS Sequence
  • >Zm00001eb366090_T002
    ATGAGGCCGCGCCTCCCTCCAAATGTTCCCTCCCTGCCTTCGTCTTTGTCGTTGCTCGCAAACTCCCTGTCCTCCCCTGTTACAAATACCCCCACCCGCCCGGACAGCTTCCCTGCATACTTGCAGCTCGCACATCTCATGGTGTCGCAGGAACGACAAGAGCCAGCTGTGCCTAGCAGCAGCAGCAGCAGCGCCAAGCGCGCAGCCACGTCCATGGACGCCAGCCCGGCCCCGCCGCTCCTCCTCCGCGCCCCCACTCCCAGCCCCAGCATTGACCTCCCCGCTGGCAAGGACAAGGCCGACGCGGCGGCCAGCAAGGCCGGCGCGGCCGTGTTCGACCTGCGCCGGGAGCCCAAGATCCCCGCGCCATTCCTGTGGCCGCAGGAAGAGGCGCGGCCGTCCTCGGCCGCGGAGCTGGAGGTGCCGATGGTGGACGTGGGCGTGCTGCGCAATGGCGACCGCGCGGGGCTGCGGCGCGCCGCGGCGCAGGTGGCCGCGGCGTGCGCGACGCACGGGTTCTTCCAGGTGTGCGGGCACGGCGTGGACGCGGCGCTGGGGCGCGCCGCGCTGGACGGCGCCAGCGACTTCTTCCGGCTGCCGCTCGCCGAGAAGCAGCGCGCCCGGCGCGTCCCCGGCACCGTGTCCGGGTACACGAGCGCGCACGCCGACCGGTTCGCGGCCAAGCTCCCCTGGAAGGAGACCCTGTCGTTCGGCTACCACGACGGCGCCGCGTCGCCTGTCGTCGTGGACTACTTCGTCGGCACCCTCGGCCAGGATTTCGAGCCAATGGGGTGGGTGTACCAGAGGTACTGCGAGGAGATGAAGGAGCTGTCGCTGACGATCATGGAGCTGCTGGAGCTGAGCCTGGGCGTGGAGCTGCGCGGCTACTACCGGGAGTTCTTCGAGGACAGCCGGTCCATCATGCGGTGCAACTACTACCCGCCGTGCCCGGAGCCGGAGCGCACGCTGGGCACGGGCCCGCACTGCGACCCCACGGCGCTCACCATCCTCCTGCAGGACGACGTGGGCGGGCTGGAGGTGCTGGTGGACGGTGAGTGGCGCCCCGTCCGGCCCGTCCCGGGCGCCATGGTCATCAACATCGGCGACACCTTCATGGTAACGAAACGAAAGCGCTCGCTCCTCTGTTTTCCTTGGCCGCTCTTGTCCTGTGTGTATATTCAGTTGAGCTCTCTCTGTGCTGTTATTTCCCGAATCCTAGTGGACCTAAACGGGCAGGTTATTACAGCACGCACACGTAGGCATGTCATGTAG
  • >Zm00001eb366090_T003
    ATGAGGCCGCGCCTCCCTCCAAATGTTCCCTCCCTGCCTTCGTCTTTGTCGTTGCTCGCAAACTCCCTGTCCTCCCCTGTTACAAATACCCCCACCCGCCCGGACAGCTTCCCTGCATACTTGCAGCTCGCACATCTCATGGTGTCGCAGGAACGACAAGAGCCAGCTGTGCCTAGCAGCAGCAGCAGCAGCGCCAAGCGCGCAGCCACGTCCATGGACGCCAGCCCGGCCCCGCCGCTCCTCCTCCGCGCCCCCACTCCCAGCCCCAGCATTGACCTCCCCGCTGGCAAGGACAAGGCCGACGCGGCGGCCAGCAAGGCCGGCGCGGCCGTGTTCGACCTGCGCCGGGAGCCCAAGATCCCCGCGCCATTCCTGTGGCCGCAGGAAGAGGCGCGGCCGTCCTCGGCCGCGGAGCTGGAGGTGCCGATGGTGGACGTGGGCGTGCTGCGCAATGGCGACCGCGCGGGGCTGCGGCGCGCCGCGGCGCAGGTGGCCGCGGCGTGCGCGACGCACGGGTTCTTCCAGGTGTGCGGGCACGGCGTGGACGCGGCGCTGGGGCGCGCCGCGCTGGACGGCGCCAGCGACTTCTTCCGGCTGCCGCTCGCCGAGAAGCAGCGCGCCCGGCGCGTCCCCGGCACCGTGTCCGGGTACACGAGCGCGCACGCCGACCGGTTCGCGGCCAAGCTCCCCTGGAAGGAGACCCTGTCGTTCGGCTACCACGACGGCGCCGCGTCGCCTGTCGTCGTGGACTACTTCGTCGGCACCCTCGGCCAGGATTTCGAGCCAATGGGGTGGGTGTACCAGAGGTACTGCGAGGAGATGAAGGAGCTGTCGCTGACGATCATGGAGCTGCTGGAGCTGAGCCTGGGCGTGGAGCTGCGCGGCTACTACCGGGAGTTCTTCGAGGACAGCCGGTCCATCATGCGGTGCAACTACTACCCGCCGTGCCCGGAGCCGGAGCGCACGCTGGGCACGGGCCCGCACTGCGACCCCACGGCGCTCACCATCCTCCTGCAGGACGACGTGGGCGGGCTGGAGGTGCTGGTGGACGGTGAGTGGCGCCCCGTCCGGCCCGTCCCGGGCGCCATGGTCATCAACATCGGCGACACCTTCATGGTAACGAAACGAAAGCGCTCGCTCCTCTGTTTTCCTTGGCCGCTCTTGTCCTGTGTGTATATTCAGTTGAGCTCTCTCTGTGCTGTTATTTCCCGAATCCTAGTGGACCTAAACGGGCAGGTTATTACAGCACGCACACGTAGGCATGTCATGTAG
  • >Zm00001eb366090_T001
    ATGAGGCCGCGCCTCCCTCCAAATGTTCCCTCCCTGCCTTCGTCTTTGTCGTTGCTCGCAAACTCCCTGTCCTCCCCTGTTACAAATACCCCCACCCGCCCGGACAGCTTCCCTGCATACTTGCAGCTCGCACATCTCATGGTGTCGCAGGAACGACAAGAGCCAGCTGTGCCTAGCAGCAGCAGCAGCAGCGCCAAGCGCGCAGCCACGTCCATGGACGCCAGCCCGGCCCCGCCGCTCCTCCTCCGCGCCCCCACTCCCAGCCCCAGCATTGACCTCCCCGCTGGCAAGGACAAGGCCGACGCGGCGGCCAGCAAGGCCGGCGCGGCCGTGTTCGACCTGCGCCGGGAGCCCAAGATCCCCGCGCCATTCCTGTGGCCGCAGGAAGAGGCGCGGCCGTCCTCGGCCGCGGAGCTGGAGGTGCCGATGGTGGACGTGGGCGTGCTGCGCAATGGCGACCGCGCGGGGCTGCGGCGCGCCGCGGCGCAGGTGGCCGCGGCGTGCGCGACGCACGGGTTCTTCCAGGTGTGCGGGCACGGCGTGGACGCGGCGCTGGGGCGCGCCGCGCTGGACGGCGCCAGCGACTTCTTCCGGCTGCCGCTCGCCGAGAAGCAGCGCGCCCGGCGCGTCCCCGGCACCGTGTCCGGGTACACGAGCGCGCACGCCGACCGGTTCGCGGCCAAGCTCCCCTGGAAGGAGACCCTGTCGTTCGGCTACCACGACGGCGCCGCGTCGCCTGTCGTCGTGGACTACTTCGTCGGCACCCTCGGCCAGGATTTCGAGCCAATGGGGTGGGTGTACCAGAGGTACTGCGAGGAGATGAAGGAGCTGTCGCTGACGATCATGGAGCTGCTGGAGCTGAGCCTGGGCGTGGAGCTGCGCGGCTACTACCGGGAGTTCTTCGAGGACAGCCGGTCCATCATGCGGTGCAACTACTACCCGCCGTGCCCGGAGCCGGAGCGCACGCTGGGCACGGGCCCGCACTGCGACCCCACGGCGCTCACCATCCTCCTGCAGGACGACGTGGGCGGGCTGGAGGTGCTGGTGGACGGTGAGTGGCGCCCCGTCCGGCCCGTCCCGGGCGCCATGGTCATCAACATCGGCGACACCTTCATGGTAACGAAACGAAAGCGCTCGCTCCTCTGTTTTCCTTGGCCGCTCTTGTCCTGTGTGTATATTCAGTTGAGCTCTCTCTGTGCTGTTATTTCCCGAATCCTAGTGGACCTAAACGGGCAGGTTATTACAGCACGCACACGTAGGCATGTCATGTAG
Protein Sequence
  • >Zm00001eb366090_P002
    MRPRLPPNVPSLPSSLSLLANSLSSPVTNTPTRPDSFPAYLQLAHLMVSQERQEPAVPSSSSSSAKRAATSMDASPAPPLLLRAPTPSPSIDLPAGKDKADAAASKAGAAVFDLRREPKIPAPFLWPQEEARPSSAAELEVPMVDVGVLRNGDRAGLRRAAAQVAAACATHGFFQVCGHGVDAALGRAALDGASDFFRLPLAEKQRARRVPGTVSGYTSAHADRFAAKLPWKETLSFGYHDGAASPVVVDYFVGTLGQDFEPMGWVYQRYCEEMKELSLTIMELLELSLGVELRGYYREFFEDSRSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDVGGLEVLVDGEWRPVRPVPGAMVINIGDTFMVTKRKRSLLCFPWPLLSCVYIQLSSLCAVISRILVDLNGQVITARTRRHVM
  • >Zm00001eb366090_P003
    MRPRLPPNVPSLPSSLSLLANSLSSPVTNTPTRPDSFPAYLQLAHLMVSQERQEPAVPSSSSSSAKRAATSMDASPAPPLLLRAPTPSPSIDLPAGKDKADAAASKAGAAVFDLRREPKIPAPFLWPQEEARPSSAAELEVPMVDVGVLRNGDRAGLRRAAAQVAAACATHGFFQVCGHGVDAALGRAALDGASDFFRLPLAEKQRARRVPGTVSGYTSAHADRFAAKLPWKETLSFGYHDGAASPVVVDYFVGTLGQDFEPMGWVYQRYCEEMKELSLTIMELLELSLGVELRGYYREFFEDSRSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDVGGLEVLVDGEWRPVRPVPGAMVINIGDTFMVTKRKRSLLCFPWPLLSCVYIQLSSLCAVISRILVDLNGQVITARTRRHVM
  • >Zm00001eb366090_P001
    MRPRLPPNVPSLPSSLSLLANSLSSPVTNTPTRPDSFPAYLQLAHLMVSQERQEPAVPSSSSSSAKRAATSMDASPAPPLLLRAPTPSPSIDLPAGKDKADAAASKAGAAVFDLRREPKIPAPFLWPQEEARPSSAAELEVPMVDVGVLRNGDRAGLRRAAAQVAAACATHGFFQVCGHGVDAALGRAALDGASDFFRLPLAEKQRARRVPGTVSGYTSAHADRFAAKLPWKETLSFGYHDGAASPVVVDYFVGTLGQDFEPMGWVYQRYCEEMKELSLTIMELLELSLGVELRGYYREFFEDSRSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDVGGLEVLVDGEWRPVRPVPGAMVINIGDTFMVTKRKRSLLCFPWPLLSCVYIQLSSLCAVISRILVDLNGQVITARTRRHVM

Source: Genetic information comes from the article