Gene Details:
- Gene ID: Zm00001eb145600
- Gene Symbol: ZmGA20ox3 ZmGA20ox2.1 ga20ox3
- Gene Name: gibberellin 20-oxidase3
- Genome: Zm-B73-REFERENCE-NAM-5.0
- Species: Zea mays
Functional Descriptions:
- To accomplish this, we utilized a dominant, miRNA-based construct expressed in a hemizygous state to selectively reduce expression of the ZmGA20ox3 and ZmGA20ox5 genes that control GA biosynthesis primarily in vegetative tissues.
- ZmGA20ox3 and ZmGA20ox5 genes that control GA biosynthesis primarily in vegetative tissues.
- Targeted suppression of gibberellin biosynthetic genes ZmGA20ox3 and ZmGA20ox5 produces a short stature maize ideotype.
- These factors identified ZmGA20ox3 and ZmGA20ox5 as the most likely candidates for gene suppression to reduce GA levels in maize stems without impacting reproductive tissues.
- Suppression of ZmGA20ox3 and ZmGA20ox5 reduces the levels of bioactive GA.
Function-related keywords:
- stems , gibberellin , vegetative , reproductive , ga , ga-biosynthetic , ga-biosynthesis , gibberellin-biosynthesis
Literature:
- Targeted suppression of gibberellin biosynthetic genes ZmGA20ox3 and ZmGA20ox5 produces a short stature maize ideotype. DOI: 10.1111/pbi.13797 ; PMID: 35244326
Related News:
Gene Resources:
- NCBI ID: LOC107521947
- UniProt accessions: A0A1D6N5E5
Sequences:
cDNA Sequence
- >Zm00001eb145600_T001
GCACACTCGCAGCTCGCACATCTCATGGTGTCCTAAGAACGGCAAGAGCCAGCTCTGCCTAGCAGCAGCGCACAGCCACATCCATGGACGCCAGCCCGACCCCACCGCTCCCCCTCCGCGCCCCAACTCCCAGCATTGACCTCCCCGCTGGCAAGGACAGGGCCGACGCGGCGGCTAACAAGGCCGCGGCTGTGTTCGACCTGCGCCGGGAGCCCAAGATCCCGGAGCCATTCCTGTGGCCGCACGAAGAGGCGCGGCCGACCTCGGCCGCGGAGCTGGAGGTGCCGGTGGTGGACGTGGGCGTGCTGCGCAATGGCGACGGCGCGGGGCTCCGCCGCGCCGCGGCGCAAGTGGCGGCGGCGTGCGCGACGCACGGGTTCTTCCAGGTGTGCGGGCACGGCGTGGACGCGGCGCTGGGGCGCGCCGCGCTGGACGGCGCCAGCGACTTCTTCCGGCTGCCGCTGGCTGAGAAGCAGCGGGCCCGGCGCGTCCCCGGCACCGTGTCCGGGTACACGAGCGCGCACGCCGACCGGTTCGCGTCCAAGCTCCCCTGGAAGGAGACCCTGTCCTTCGGCTTCCACGACGGCGCCGCGGCGCCCGTCGTCGTGGACTACTTCACCGGCACCCTCGGCCAAGATTTCGAGCCAGTGGGGCGGGTGTACCAGAGGTACTGCGAGGAGATGAAGGAGCTGTCGCTGACGATCATGGAGCTGCTGGAGCTGAGCCTGGGCGTGGAGCGCGGCTACTACCGGGAGTTCTTCGAGGACAGCCGCTCCATCATGCGGACGACGTCGGCGGGCTGGAGGTCCTGGTGGACGGCGAGTGGCGCCCCGTCCGGCCCGTCCCAGGCGCCATGGTCATCAACATCGGCGACACCTTCATGGCGCTGTCCAACGGGCGGTACAAGAGCTGCCTGCACCGCGCGGTGGTGAACCGGCGGCAGGAGCGGCAATCGCTGGCCTTCTTCCTGTGCCCGCGCGAGGACCGGGTGGTGCGCCCGCCGGCCAGCGCCGCGCCGCGGCAGTACCCGGACTTCACCTGGGCCGACCTCATGCGCTTCACGCAGCGCCACTACCGCGCCGACACCCGCACGCTGGACGCCTTCACCCGCTGGCTCTCCCACGGCCCGGCGGCGGCGGCTCCCTGCACCTAACGAGCCGGCCGTCTCTTTCGCCGGGGCCCGCGCGGGGTTCGCCCACGTGGTGATCAGGTGGCAGACATGTGGCCCACGGGCCCCGCGCCGCCTTCCCCATTTTTGGACGACCCTACTGCTACTACTACTAGTGTACATATGCAAAAAAATACATATATATATAGGTACTTTCTCTAATATTTTTATATATAAGCAAGGCGGCCTGGTGTTCTTTTCTTTGTTTTGTCGACAACTGTTTGATCCCATCCTATGGACGATGGATAGTTCAATGT - >Zm00001eb145600_T002
GCACACTCGCAGCTCGCACATCTCATGGTGTCCTAAGAACGGCAAGAGCCAGCTCTGCCTAGCAGCAGCGCACAGCCACATCCATGGACGCCAGCCCGACCCCACCGCTCCCCCTCCGCGCCCCAACTCCCAGCATTGACCTCCCCGCTGGCAAGGACAGGGCCGACGCGGCGGCTAACAAGGCCGCGGCTGTGTTCGACCTGCGCCGGGAGCCCAAGATCCCGGAGCCATTCCTGTGGCCGCACGAAGAGGCGCGGCCGACCTCGGCCGCGGAGCTGGAGGTGCCGGTGGTGGACGTGGGCGTGCTGCGCAATGGCGACGGCGCGGGGCTCCGCCGCGCCGCGGCGCAAGTGGCGGCGGCGTGCGCGACGCACGGGTTCTTCCAGGTGTGCGGGCACGGCGTGGACGCGGCGCTGGGGCGCGCCGCGCTGGACGGCGCCAGCGACTTCTTCCGGCTGCCGCTGGCTGAGAAGCAGCGGGCCCGGCGCGTCCCCGGCACCGTGTCCGGGTACACGAGCGCGCACGCCGACCGGTTCGCGTCCAAGCTCCCCTGGAAGGAGACCCTGTCCTTCGGCTTCCACGACGGCGCCGCGGCGCCCGTCGTCGTGGACTACTTCACCGGCACCCTCGGCCAAGATTTCGAGCCAGTGGGGCGGGTGTACCAGAGGTACTGCGAGGAGATGAAGGAGCTGTCGCTGACGATCATGGAGCTGCTGGAGCTGAGCCTGGGCGTGGAGCGCGGCTACTACCGGGAGTTCTTCGAGGACAGCCGCTCCATCATGCGGACGACGTCGGCGGGCTGGAGGTCCTGGTGGACGGCGAGTGGCGCCCCGTCCGGCCCGTCCCAGGCGCCATGGTCATCAACATCGGCGACACCTTCATGGCGCTGTCCAACGGGCGGTACAAGAGCTGCCTGCACCGCGCGGTGGTGAACCGGCGGCAGGAGCGGCAATCGCTGGCCTTCTTCCTGTGCCCGCGCGAGGACCGGGTGGTGCGCCCGCCGGCCAGCGCCGCGCCGCGGCAGTACCCGGACTTCACCTGGGCCGACCTCATGCGCTTCACGCAGCGCCACTACCGCGCCGACACCCGCACGCTGGACGCCTTCACCCGCTGGCTCTCCCACGGCCCGGCGGCGGCGGCTCCCTGCACCTAACGAGCCGGCCGTCTCTTTCGCCGGGGCCCGCGCGGGGTTCGCCCACGTGGTGATCAGGTGGCAGACATGTGGCCCACGGGCCCCGCGCCGCCTTCCCCATTTTTGGACGACCCTACTGCTACTACTACTAGTGTACATATGCAAAAAAATACATATATATATAGGTACTTTCTCTAATATTTTTATATATAAGCAAGGCGGCCTGGTGTTCTTTTCTTTGTTTTGTCGACAACTGTTTGATCCCATCCTATGGACGATGGATAGTTCAATGT
CDS Sequence
- >Zm00001eb145600_T001
ATGGACGCCAGCCCGACCCCACCGCTCCCCCTCCGCGCCCCAACTCCCAGCATTGACCTCCCCGCTGGCAAGGACAGGGCCGACGCGGCGGCTAACAAGGCCGCGGCTGTGTTCGACCTGCGCCGGGAGCCCAAGATCCCGGAGCCATTCCTGTGGCCGCACGAAGAGGCGCGGCCGACCTCGGCCGCGGAGCTGGAGGTGCCGGTGGTGGACGTGGGCGTGCTGCGCAATGGCGACGGCGCGGGGCTCCGCCGCGCCGCGGCGCAAGTGGCGGCGGCGTGCGCGACGCACGGGTTCTTCCAGGTGTGCGGGCACGGCGTGGACGCGGCGCTGGGGCGCGCCGCGCTGGACGGCGCCAGCGACTTCTTCCGGCTGCCGCTGGCTGAGAAGCAGCGGGCCCGGCGCGTCCCCGGCACCGTGTCCGGGTACACGAGCGCGCACGCCGACCGGTTCGCGTCCAAGCTCCCCTGGAAGGAGACCCTGTCCTTCGGCTTCCACGACGGCGCCGCGGCGCCCGTCGTCGTGGACTACTTCACCGGCACCCTCGGCCAAGATTTCGAGCCAGTGGGGCGGGTGTACCAGAGGTACTGCGAGGAGATGAAGGAGCTGTCGCTGACGATCATGGAGCTGCTGGAGCTGAGCCTGGGCGTGGAGCGCGGCTACTACCGGGAGTTCTTCGAGGACAGCCGCTCCATCATGCGGACGACGTCGGCGGGCTGGAGGTCCTGGTGGACGGCGAGTGGCGCCCCGTCCGGCCCGTCCCAGGCGCCATGGTCATCAACATCGGCGACACCTTCATGGCGCTGTCCAACGGGCGGTACAAGAGCTGCCTGCACCGCGCGGTGGTGA - >Zm00001eb145600_T002
ATGGACGCCAGCCCGACCCCACCGCTCCCCCTCCGCGCCCCAACTCCCAGCATTGACCTCCCCGCTGGCAAGGACAGGGCCGACGCGGCGGCTAACAAGGCCGCGGCTGTGTTCGACCTGCGCCGGGAGCCCAAGATCCCGGAGCCATTCCTGTGGCCGCACGAAGAGGCGCGGCCGACCTCGGCCGCGGAGCTGGAGGTGCCGGTGGTGGACGTGGGCGTGCTGCGCAATGGCGACGGCGCGGGGCTCCGCCGCGCCGCGGCGCAAGTGGCGGCGGCGTGCGCGACGCACGGGTTCTTCCAGGTGTGCGGGCACGGCGTGGACGCGGCGCTGGGGCGCGCCGCGCTGGACGGCGCCAGCGACTTCTTCCGGCTGCCGCTGGCTGAGAAGCAGCGGGCCCGGCGCGTCCCCGGCACCGTGTCCGGGTACACGAGCGCGCACGCCGACCGGTTCGCGTCCAAGCTCCCCTGGAAGGAGACCCTGTCCTTCGGCTTCCACGACGGCGCCGCGGCGCCCGTCGTCGTGGACTACTTCACCGGCACCCTCGGCCAAGATTTCGAGCCAGTGGGGCGGGTGTACCAGAGGTACTGCGAGGAGATGAAGGAGCTGTCGCTGACGATCATGGAGCTGCTGGAGCTGAGCCTGGGCGTGGAGCGCGGCTACTACCGGGAGTTCTTCGAGGACAGCCGCTCCATCATGCGGACGACGTCGGCGGGCTGGAGGTCCTGGTGGACGGCGAGTGGCGCCCCGTCCGGCCCGTCCCAGGCGCCATGGTCATCAACATCGGCGACACCTTCATGGCGCTGTCCAACGGGCGGTACAAGAGCTGCCTGCACCGCGCGGTGGTGA
Protein Sequence
- >Zm00001eb145600_P001
MDASPTPPLPLRAPTPSIDLPAGKDRADAAANKAAAVFDLRREPKIPEPFLWPHEEARPTSAAELEVPVVDVGVLRNGDGAGLRRAAAQVAAACATHGFFQVCGHGVDAALGRAALDGASDFFRLPLAEKQRARRVPGTVSGYTSAHADRFASKLPWKETLSFGFHDGAAAPVVVDYFTGTLGQDFEPVGRVYQRYCEEMKELSLTIMELLELSLGVERGYYREFFEDSRSIMRTTSAGWRSWWTASGAPSGPSQAPWSSTSATPSWRCPTGGTRAACTARW - >Zm00001eb145600_P002
MDASPTPPLPLRAPTPSIDLPAGKDRADAAANKAAAVFDLRREPKIPEPFLWPHEEARPTSAAELEVPVVDVGVLRNGDGAGLRRAAAQVAAACATHGFFQVCGHGVDAALGRAALDGASDFFRLPLAEKQRARRVPGTVSGYTSAHADRFASKLPWKETLSFGFHDGAAAPVVVDYFTGTLGQDFEPVGRVYQRYCEEMKELSLTIMELLELSLGVERGYYREFFEDSRSIMRTTSAGWRSWWTASGAPSGPSQAPWSSTSATPSWRCPTGGTRAACTARW
Source: Genetic information comes from the article