Gene Details:

Functional Descriptions:

  • To accomplish this, we utilized a dominant, miRNA-based construct expressed in a hemizygous state to selectively reduce expression of the ZmGA20ox3 and ZmGA20ox5 genes that control GA biosynthesis primarily in vegetative tissues.
  • ZmGA20ox3 and ZmGA20ox5 genes that control GA biosynthesis primarily in vegetative tissues.
  • Targeted suppression of gibberellin biosynthetic genes ZmGA20ox3 and ZmGA20ox5 produces a short stature maize ideotype.
  • These factors identified ZmGA20ox3 and ZmGA20ox5 as the most likely candidates for gene suppression to reduce GA levels in maize stems without impacting reproductive tissues.
  • Suppression of ZmGA20ox3 and ZmGA20ox5 reduces the levels of bioactive GA.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Zm00001eb145600_T001
    GCACACTCGCAGCTCGCACATCTCATGGTGTCCTAAGAACGGCAAGAGCCAGCTCTGCCTAGCAGCAGCGCACAGCCACATCCATGGACGCCAGCCCGACCCCACCGCTCCCCCTCCGCGCCCCAACTCCCAGCATTGACCTCCCCGCTGGCAAGGACAGGGCCGACGCGGCGGCTAACAAGGCCGCGGCTGTGTTCGACCTGCGCCGGGAGCCCAAGATCCCGGAGCCATTCCTGTGGCCGCACGAAGAGGCGCGGCCGACCTCGGCCGCGGAGCTGGAGGTGCCGGTGGTGGACGTGGGCGTGCTGCGCAATGGCGACGGCGCGGGGCTCCGCCGCGCCGCGGCGCAAGTGGCGGCGGCGTGCGCGACGCACGGGTTCTTCCAGGTGTGCGGGCACGGCGTGGACGCGGCGCTGGGGCGCGCCGCGCTGGACGGCGCCAGCGACTTCTTCCGGCTGCCGCTGGCTGAGAAGCAGCGGGCCCGGCGCGTCCCCGGCACCGTGTCCGGGTACACGAGCGCGCACGCCGACCGGTTCGCGTCCAAGCTCCCCTGGAAGGAGACCCTGTCCTTCGGCTTCCACGACGGCGCCGCGGCGCCCGTCGTCGTGGACTACTTCACCGGCACCCTCGGCCAAGATTTCGAGCCAGTGGGGCGGGTGTACCAGAGGTACTGCGAGGAGATGAAGGAGCTGTCGCTGACGATCATGGAGCTGCTGGAGCTGAGCCTGGGCGTGGAGCGCGGCTACTACCGGGAGTTCTTCGAGGACAGCCGCTCCATCATGCGGACGACGTCGGCGGGCTGGAGGTCCTGGTGGACGGCGAGTGGCGCCCCGTCCGGCCCGTCCCAGGCGCCATGGTCATCAACATCGGCGACACCTTCATGGCGCTGTCCAACGGGCGGTACAAGAGCTGCCTGCACCGCGCGGTGGTGAACCGGCGGCAGGAGCGGCAATCGCTGGCCTTCTTCCTGTGCCCGCGCGAGGACCGGGTGGTGCGCCCGCCGGCCAGCGCCGCGCCGCGGCAGTACCCGGACTTCACCTGGGCCGACCTCATGCGCTTCACGCAGCGCCACTACCGCGCCGACACCCGCACGCTGGACGCCTTCACCCGCTGGCTCTCCCACGGCCCGGCGGCGGCGGCTCCCTGCACCTAACGAGCCGGCCGTCTCTTTCGCCGGGGCCCGCGCGGGGTTCGCCCACGTGGTGATCAGGTGGCAGACATGTGGCCCACGGGCCCCGCGCCGCCTTCCCCATTTTTGGACGACCCTACTGCTACTACTACTAGTGTACATATGCAAAAAAATACATATATATATAGGTACTTTCTCTAATATTTTTATATATAAGCAAGGCGGCCTGGTGTTCTTTTCTTTGTTTTGTCGACAACTGTTTGATCCCATCCTATGGACGATGGATAGTTCAATGT
  • >Zm00001eb145600_T002
    GCACACTCGCAGCTCGCACATCTCATGGTGTCCTAAGAACGGCAAGAGCCAGCTCTGCCTAGCAGCAGCGCACAGCCACATCCATGGACGCCAGCCCGACCCCACCGCTCCCCCTCCGCGCCCCAACTCCCAGCATTGACCTCCCCGCTGGCAAGGACAGGGCCGACGCGGCGGCTAACAAGGCCGCGGCTGTGTTCGACCTGCGCCGGGAGCCCAAGATCCCGGAGCCATTCCTGTGGCCGCACGAAGAGGCGCGGCCGACCTCGGCCGCGGAGCTGGAGGTGCCGGTGGTGGACGTGGGCGTGCTGCGCAATGGCGACGGCGCGGGGCTCCGCCGCGCCGCGGCGCAAGTGGCGGCGGCGTGCGCGACGCACGGGTTCTTCCAGGTGTGCGGGCACGGCGTGGACGCGGCGCTGGGGCGCGCCGCGCTGGACGGCGCCAGCGACTTCTTCCGGCTGCCGCTGGCTGAGAAGCAGCGGGCCCGGCGCGTCCCCGGCACCGTGTCCGGGTACACGAGCGCGCACGCCGACCGGTTCGCGTCCAAGCTCCCCTGGAAGGAGACCCTGTCCTTCGGCTTCCACGACGGCGCCGCGGCGCCCGTCGTCGTGGACTACTTCACCGGCACCCTCGGCCAAGATTTCGAGCCAGTGGGGCGGGTGTACCAGAGGTACTGCGAGGAGATGAAGGAGCTGTCGCTGACGATCATGGAGCTGCTGGAGCTGAGCCTGGGCGTGGAGCGCGGCTACTACCGGGAGTTCTTCGAGGACAGCCGCTCCATCATGCGGACGACGTCGGCGGGCTGGAGGTCCTGGTGGACGGCGAGTGGCGCCCCGTCCGGCCCGTCCCAGGCGCCATGGTCATCAACATCGGCGACACCTTCATGGCGCTGTCCAACGGGCGGTACAAGAGCTGCCTGCACCGCGCGGTGGTGAACCGGCGGCAGGAGCGGCAATCGCTGGCCTTCTTCCTGTGCCCGCGCGAGGACCGGGTGGTGCGCCCGCCGGCCAGCGCCGCGCCGCGGCAGTACCCGGACTTCACCTGGGCCGACCTCATGCGCTTCACGCAGCGCCACTACCGCGCCGACACCCGCACGCTGGACGCCTTCACCCGCTGGCTCTCCCACGGCCCGGCGGCGGCGGCTCCCTGCACCTAACGAGCCGGCCGTCTCTTTCGCCGGGGCCCGCGCGGGGTTCGCCCACGTGGTGATCAGGTGGCAGACATGTGGCCCACGGGCCCCGCGCCGCCTTCCCCATTTTTGGACGACCCTACTGCTACTACTACTAGTGTACATATGCAAAAAAATACATATATATATAGGTACTTTCTCTAATATTTTTATATATAAGCAAGGCGGCCTGGTGTTCTTTTCTTTGTTTTGTCGACAACTGTTTGATCCCATCCTATGGACGATGGATAGTTCAATGT
CDS Sequence
  • >Zm00001eb145600_T001
    ATGGACGCCAGCCCGACCCCACCGCTCCCCCTCCGCGCCCCAACTCCCAGCATTGACCTCCCCGCTGGCAAGGACAGGGCCGACGCGGCGGCTAACAAGGCCGCGGCTGTGTTCGACCTGCGCCGGGAGCCCAAGATCCCGGAGCCATTCCTGTGGCCGCACGAAGAGGCGCGGCCGACCTCGGCCGCGGAGCTGGAGGTGCCGGTGGTGGACGTGGGCGTGCTGCGCAATGGCGACGGCGCGGGGCTCCGCCGCGCCGCGGCGCAAGTGGCGGCGGCGTGCGCGACGCACGGGTTCTTCCAGGTGTGCGGGCACGGCGTGGACGCGGCGCTGGGGCGCGCCGCGCTGGACGGCGCCAGCGACTTCTTCCGGCTGCCGCTGGCTGAGAAGCAGCGGGCCCGGCGCGTCCCCGGCACCGTGTCCGGGTACACGAGCGCGCACGCCGACCGGTTCGCGTCCAAGCTCCCCTGGAAGGAGACCCTGTCCTTCGGCTTCCACGACGGCGCCGCGGCGCCCGTCGTCGTGGACTACTTCACCGGCACCCTCGGCCAAGATTTCGAGCCAGTGGGGCGGGTGTACCAGAGGTACTGCGAGGAGATGAAGGAGCTGTCGCTGACGATCATGGAGCTGCTGGAGCTGAGCCTGGGCGTGGAGCGCGGCTACTACCGGGAGTTCTTCGAGGACAGCCGCTCCATCATGCGGACGACGTCGGCGGGCTGGAGGTCCTGGTGGACGGCGAGTGGCGCCCCGTCCGGCCCGTCCCAGGCGCCATGGTCATCAACATCGGCGACACCTTCATGGCGCTGTCCAACGGGCGGTACAAGAGCTGCCTGCACCGCGCGGTGGTGA
  • >Zm00001eb145600_T002
    ATGGACGCCAGCCCGACCCCACCGCTCCCCCTCCGCGCCCCAACTCCCAGCATTGACCTCCCCGCTGGCAAGGACAGGGCCGACGCGGCGGCTAACAAGGCCGCGGCTGTGTTCGACCTGCGCCGGGAGCCCAAGATCCCGGAGCCATTCCTGTGGCCGCACGAAGAGGCGCGGCCGACCTCGGCCGCGGAGCTGGAGGTGCCGGTGGTGGACGTGGGCGTGCTGCGCAATGGCGACGGCGCGGGGCTCCGCCGCGCCGCGGCGCAAGTGGCGGCGGCGTGCGCGACGCACGGGTTCTTCCAGGTGTGCGGGCACGGCGTGGACGCGGCGCTGGGGCGCGCCGCGCTGGACGGCGCCAGCGACTTCTTCCGGCTGCCGCTGGCTGAGAAGCAGCGGGCCCGGCGCGTCCCCGGCACCGTGTCCGGGTACACGAGCGCGCACGCCGACCGGTTCGCGTCCAAGCTCCCCTGGAAGGAGACCCTGTCCTTCGGCTTCCACGACGGCGCCGCGGCGCCCGTCGTCGTGGACTACTTCACCGGCACCCTCGGCCAAGATTTCGAGCCAGTGGGGCGGGTGTACCAGAGGTACTGCGAGGAGATGAAGGAGCTGTCGCTGACGATCATGGAGCTGCTGGAGCTGAGCCTGGGCGTGGAGCGCGGCTACTACCGGGAGTTCTTCGAGGACAGCCGCTCCATCATGCGGACGACGTCGGCGGGCTGGAGGTCCTGGTGGACGGCGAGTGGCGCCCCGTCCGGCCCGTCCCAGGCGCCATGGTCATCAACATCGGCGACACCTTCATGGCGCTGTCCAACGGGCGGTACAAGAGCTGCCTGCACCGCGCGGTGGTGA
Protein Sequence
  • >Zm00001eb145600_P001
    MDASPTPPLPLRAPTPSIDLPAGKDRADAAANKAAAVFDLRREPKIPEPFLWPHEEARPTSAAELEVPVVDVGVLRNGDGAGLRRAAAQVAAACATHGFFQVCGHGVDAALGRAALDGASDFFRLPLAEKQRARRVPGTVSGYTSAHADRFASKLPWKETLSFGFHDGAAAPVVVDYFTGTLGQDFEPVGRVYQRYCEEMKELSLTIMELLELSLGVERGYYREFFEDSRSIMRTTSAGWRSWWTASGAPSGPSQAPWSSTSATPSWRCPTGGTRAACTARW
  • >Zm00001eb145600_P002
    MDASPTPPLPLRAPTPSIDLPAGKDRADAAANKAAAVFDLRREPKIPEPFLWPHEEARPTSAAELEVPVVDVGVLRNGDGAGLRRAAAQVAAACATHGFFQVCGHGVDAALGRAALDGASDFFRLPLAEKQRARRVPGTVSGYTSAHADRFASKLPWKETLSFGFHDGAAAPVVVDYFTGTLGQDFEPVGRVYQRYCEEMKELSLTIMELLELSLGVERGYYREFFEDSRSIMRTTSAGWRSWWTASGAPSGPSQAPWSSTSATPSWRCPTGGTRAACTARW

Source: Genetic information comes from the article