Gene Details:

Functional Descriptions:

  • Chloride content analysis revealed that ZmCLCg regulated chloride transport under sodium chloride stress. These results help to explain genetic variations in salt tolerance and provide novel loci for generating salt-tolerant maize lines.
  • Under 100 mm NaCl treatment, the chloride content in shoots of three ZmCLCg mutants was significantly higher than that of wild type (Figure 4i), indicating that the salt tolerance function of ZmCLCg was associated with chloride transport.
  • By comparing the biomass of three CRISPR/Cas9 mutants of ZmCLCg and one zmpmp3 EMS mutant with their wild-type plants under salt stress, the salt tolerance function of candidate genes ZmCLCg and ZmPMP3 were confirmed.
  • The biomass of three ZmCLCg mutants and one zmpmp3 EMS mutant were compared with their wild-type plants under 100 mm NaCl treatment, respectively, and the results showed that the root length, root fresh weight, shoot length, shoot fresh weight of all mutants were significantly lower than that of the wild type, which verified the salt tolerance function of both ZmPMP3 and ZmCLCg.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Zm00001eb068640_T002
    CCACAATCTCTCTGTCCCGGGAGTCCGTCCTTTCTGCTCTGTCGTTACTGAAACCCAGCGAGCACCACTTCCCATCTGCTCGTGTCAGTCAGTTCCTCGGCTGTGCCGTGAGTGGCGGCGGCAGGGAGCGGCGCAGCGCAGCGCAGAAGCAGCGGAGTGGCCCAGGCGATGGCGCCGAGGGACCCCAAGGAGGACGGCTTCGGCGTGGGCGGCGCGGCGGATCCGGAGGCCGACATCGAGGCCCCGCTGCTGGTTTCCTCCGGATCATCGTTCCTCCTGGACCCGGCGCTCGAGGACGGCGGCGGCGGCGACGAGGAGCAGCGCCGCCGCCGCCGCCGGTTCCTCCACGGCAGCCACACGCACTCCAACACCACCTCCCAGGTCGCGCTCGTCGGCGCCGACGTCTGCCCCATCGAGAGCCTCGACTACGAGCTGATCGAGAACGACGTGTTCAAGCAGGACTGGAGGGCGCGGGGACGGGGCCACATCCTGCGCTACGTGGCGCTCAAGTGGGCCCTCTGCTTCCTCGTCGGTGCCCTCGCAGCCGCCGCCGGCTTCGTCGCCAACCTCGGCGTCGAGAATGTCGCCGGCGCCAAGTTCGTCGTCACCTCCGACCTCATGTTGGACGGCAGACACGGCTCGGCGTTCGCGGTGTTCCTGGCGTCCAACTTTGCGCTCACCATGTTAGCCGCGGTGCTGACGGTGTACGTGGCGCCTGCGGCTGCCGGATCCGGAATCCCGGAGGTGAAGGCCTACTTGAACGGTGTTGATGCCCCCAACATATTCTCACTGAAAACTCTGATAGTGAAGATTGTGGGATGCATTGCGGCAGTATCATCATCACTGCATGTGGGAAAAGCTGGGCCACTTGTACACACAGGCGCATGCATTGCATCAATACTTGGGCAAGGTGGGTCACGTAAATATCGTATGACATGTAGGTGGCTCAGGTACTTCAAGAATGATAGGGATCGGAGGGACCTTGTTACTTGTGGTTCTGCTGCTGGTATTGCTGCTGCTTTCCGGGCGCCGGTTGGTGGAGTCCTTTTTGCTCTGGAAACAGTATCTTCATGGTAAATTCTTTGAAAATGTGTTTCATGATGTATTTATGTAACTCTTGTTTATTACTATTGTGGAGCG
  • >Zm00001eb068640_T001
    CCACAATCTCTCTGTCCCGGGAGTCCGTCCTTTCTGCTCTGTCGTTACTGAAACCCAGCGAGCACCACTTCCCATCTGCTCGTGTCAGTCAGTTCCTCGGCTGTGCCGTGAGTGGCGGCGGCAGGGAGCGGCGCAGCGCAGCGCAGAAGCAGCGGAGTGGCCCAGGCGATGGCGCCGAGGGACCCCAAGGAGGACGGCTTCGGCGTGGGCGGCGCGGCGGATCCGGAGGCCGACATCGAGGCCCCGCTGCTGGTTTCCTCCGGATCATCGTTCCTCCTGGACCCGGCGCTCGAGGACGGCGGCGGCGGCGACGAGGAGCAGCGCCGCCGCCGCCGCCGGTTCCTCCACGGCAGCCACACGCACTCCAACACCACCTCCCAGGTCGCGCTCGTCGGCGCCGACGTCTGCCCCATCGAGAGCCTCGACTACGAGCTGATCGAGAACGACGTGTTCAAGCAGGACTGGAGGGCGCGGGGACGGGGCCACATCCTGCGCTACGTGGCGCTCAAGTGGGCCCTCTGCTTCCTCGTCGGTGCCCTCGCAGCCGCCGCCGGCTTCGTCGCCAACCTCGGCGTCGAGAATGTCGCCGGCGCCAAGTTCGTCGTCACCTCCGACCTCATGTTGGACGGCAGACACGGCTCGGCGTTCGCGGTGTTCCTGGCGTCCAACTTTGCGCTCACCATGTTAGCCGCGGTGCTGACGGTGTACGTGGCGCCTGCGGCTGCCGGATCCGGAATCCCGGAGGTGAAGGCCTACTTGAACGGTGTTGATGCCCCCAACATATTCTCACTGAAAACTCTGATAGTGAAGATTGTGGGATGCATTGCGGCAGTATCATCATCACTGCATGTGGGAAAAGCTGGGCCACTTGTACACACAGGCGCATGCATTGCATCAATACTTGGGCAAGGTGGGTCACGTAAATATCGTATGACATGTAGGTGGCTCAGGTACTTCAAGAATGATAGGGATCGGAGGGACCTTGTTACTTGTGGTTCTGCTGCTGGTATTGCTGCTGCTTTCCGGGCGCCGGTTGGTGGAGTCCTTTTTGCTCTGGAAACAGTATCTTCATGGTAAATTCTTTGAAAATGTGTTTCATGATGTATTTATGTAACTCTTGTTTATTACTATTGTGGAGCG
CDS Sequence
  • >Zm00001eb068640_T002
    ATGGCGCCGAGGGACCCCAAGGAGGACGGCTTCGGCGTGGGCGGCGCGGCGGATCCGGAGGCCGACATCGAGGCCCCGCTGCTGGTTTCCTCCGGATCATCGTTCCTCCTGGACCCGGCGCTCGAGGACGGCGGCGGCGGCGACGAGGAGCAGCGCCGCCGCCGCCGCCGGTTCCTCCACGGCAGCCACACGCACTCCAACACCACCTCCCAGGTCGCGCTCGTCGGCGCCGACGTCTGCCCCATCGAGAGCCTCGACTACGAGCTGATCGAGAACGACGTGTTCAAGCAGGACTGGAGGGCGCGGGGACGGGGCCACATCCTGCGCTACGTGGCGCTCAAGTGGGCCCTCTGCTTCCTCGTCGGTGCCCTCGCAGCCGCCGCCGGCTTCGTCGCCAACCTCGGCGTCGAGAATGTCGCCGGCGCCAAGTTCGTCGTCACCTCCGACCTCATGTTGGACGGCAGACACGGCTCGGCGTTCGCGGTGTTCCTGGCGTCCAACTTTGCGCTCACCATGTTAGCCGCGGTGCTGACGGTGTACGTGGCGCCTGCGGCTGCCGGATCCGGAATCCCGGAGGTGAAGGCCTACTTGAACGGTGTTGATGCCCCCAACATATTCTCACTGAAAACTCTGATAGTGAAGATTGTGGGATGCATTGCGGCAGTATCATCATCACTGCATGTGGGAAAAGCTGGGCCACTTGTACACACAGGCGCATGCATTGCATCAATACTTGGGCAAGGTGGGTCACGTAAATATCGTATGACATGTAGGTGGCTCAGGTACTTCAAGAATGATAGGGATCGGAGGGACCTTGTTACTTGTGGTTCTGCTGCTGGTATTGCTGCTGCTTTCCGGGCGCCGGTTGGTGGAGTCCTTTTTGCTCTGGAAACAGTATCTTCATGGTAA
  • >Zm00001eb068640_T001
    ATGGCGCCGAGGGACCCCAAGGAGGACGGCTTCGGCGTGGGCGGCGCGGCGGATCCGGAGGCCGACATCGAGGCCCCGCTGCTGGTTTCCTCCGGATCATCGTTCCTCCTGGACCCGGCGCTCGAGGACGGCGGCGGCGGCGACGAGGAGCAGCGCCGCCGCCGCCGCCGGTTCCTCCACGGCAGCCACACGCACTCCAACACCACCTCCCAGGTCGCGCTCGTCGGCGCCGACGTCTGCCCCATCGAGAGCCTCGACTACGAGCTGATCGAGAACGACGTGTTCAAGCAGGACTGGAGGGCGCGGGGACGGGGCCACATCCTGCGCTACGTGGCGCTCAAGTGGGCCCTCTGCTTCCTCGTCGGTGCCCTCGCAGCCGCCGCCGGCTTCGTCGCCAACCTCGGCGTCGAGAATGTCGCCGGCGCCAAGTTCGTCGTCACCTCCGACCTCATGTTGGACGGCAGACACGGCTCGGCGTTCGCGGTGTTCCTGGCGTCCAACTTTGCGCTCACCATGTTAGCCGCGGTGCTGACGGTGTACGTGGCGCCTGCGGCTGCCGGATCCGGAATCCCGGAGGTGAAGGCCTACTTGAACGGTGTTGATGCCCCCAACATATTCTCACTGAAAACTCTGATAGTGAAGATTGTGGGATGCATTGCGGCAGTATCATCATCACTGCATGTGGGAAAAGCTGGGCCACTTGTACACACAGGCGCATGCATTGCATCAATACTTGGGCAAGGTGGGTCACGTAAATATCGTATGACATGTAGGTGGCTCAGGTACTTCAAGAATGATAGGGATCGGAGGGACCTTGTTACTTGTGGTTCTGCTGCTGGTATTGCTGCTGCTTTCCGGGCGCCGGTTGGTGGAGTCCTTTTTGCTCTGGAAACAGTATCTTCATGGTAA
Protein Sequence
  • >Zm00001eb068640_P002
    MAPRDPKEDGFGVGGAADPEADIEAPLLVSSGSSFLLDPALEDGGGGDEEQRRRRRRFLHGSHTHSNTTSQVALVGADVCPIESLDYELIENDVFKQDWRARGRGHILRYVALKWALCFLVGALAAAAGFVANLGVENVAGAKFVVTSDLMLDGRHGSAFAVFLASNFALTMLAAVLTVYVAPAAAGSGIPEVKAYLNGVDAPNIFSLKTLIVKIVGCIAAVSSSLHVGKAGPLVHTGACIASILGQGGSRKYRMTCRWLRYFKNDRDRRDLVTCGSAAGIAAAFRAPVGGVLFALETVSSW
  • >Zm00001eb068640_P001
    MAPRDPKEDGFGVGGAADPEADIEAPLLVSSGSSFLLDPALEDGGGGDEEQRRRRRRFLHGSHTHSNTTSQVALVGADVCPIESLDYELIENDVFKQDWRARGRGHILRYVALKWALCFLVGALAAAAGFVANLGVENVAGAKFVVTSDLMLDGRHGSAFAVFLASNFALTMLAAVLTVYVAPAAAGSGIPEVKAYLNGVDAPNIFSLKTLIVKIVGCIAAVSSSLHVGKAGPLVHTGACIASILGQGGSRKYRMTCRWLRYFKNDRDRRDLVTCGSAAGIAAAFRAPVGGVLFALETVSSW

Source: Genetic information comes from the article