Gene Details:
- Gene ID: Zm00001eb041930
- Gene Symbol: ZmBADH2b aldh9
- Gene Name: aldehyde dehydrogenase9
- Genome: Zm-B73-REFERENCE-NAM-5.0
- Species: Zea mays
Functional Descriptions:
- These results suggest that ZmBADH2a and ZmBADH2b redundantly participate in 2AP biosynthesis in maize, and represent the creation of the world’s first aromatic maize by simultaneous genome editing of the two BADH2 genes.
- Here, we characterized the two maize BADH2 homologs, ZmBADH2a and ZmBADH2b. We generated zmbadh2a and ZmBADH2b single mutants and the zmbadh2a-ZmBADH2b double mutant by CRISPR/Cas in four inbred lines. A popcorn-like scent was only noticeable in seeds from the double mutant, but not from either single mutant or in wild type.
- These results demonstrated that ZmBADH2a and ZmBADH2b are both involved in 2AP biosynthesis and only their combined inactivation results in 2AP accumulation in maize.
Function-related keywords:
Literature:
- Creation of aromatic maize by CRISPR/Cas. DOI: 10.1111/jipb.13105 ; PMID: 33934500
- Genome-Wide Characterization of the Aldehyde Dehydrogenase Gene Superfamily in Maize and Its Potential Role in Anther Development DOI: 10.1021/acsagscitech.3c00430 ; PMID:
Related News:
Gene Resources:
- NCBI ID: LOC606443
- UniProt accessions: A0A1D6KPH6 , A0A1D6KPH7 , A0A1D6KPH8 , G5DDC2
Sequences:
cDNA Sequence
- >Zm00001eb041930_T002
ATAGCCTACACTACACAGGGCCGCAGCTTGCCGATCCCTAGCGACGAAGTCCACTGCCGAGTTGCCGACTCACCGACTCCCCTCGCAAGTCGCAATCTCCACTCTCCAGAGTCCAAACTCCACTCCTCACCGATGATGGCCTCGCAAGCGATGGTACCGCTGCGGCAGCTCTTTGTCGACGGCGAGTGGCGCCCGCCCGCGCAGGGCCGCCGCCTCCCCGTCGTCAACCCTACCACTGAGGCTCACATCGGCGAGATCCCGGCGGGCACGGCGGAGGACGTGGACGCCGCGGTGGCGGCGGCGCGCGCGGCGCTCAAGAGGAACCGCGGCCGCGATTGGGCGCGCGCGCCGGGGGCCGTTCGGGCCAAGTACCTCCGCGCCATCGCCGCCAAGGTAATTGAGAGGAAACAAGAGCTAGCTAAGCTAGAGGCACTTGATTGTGGGAAGCCTTATGATGAAGCTGCATGGGACATGGATGATGTTGCTGGGTGCTTTGAGTACTTCGCAGATCAGGCAGAAGCTTTGGACAAAAGACAAAATTCCCCAGTTTCTCTTCCGATGGAAACTTTTAAATGCCATCTCCGAAGAGAGCCTATTGGGGTAGTTGGGCTGATAACTCCTTGGAACTATCCTCTCCTGATGGCTACATGGAAGGTAGCTCCTGCTCTGGCTGCTGGTTGTGCAGCTGTGCTAAAGCCATCTGAATTGGCTTCTGTGACTTGCTTAGAGCTTGCTGATATCTGTAAAGAAGTCGGTCTTCCTCCCGGTGTCTTAAACATTGTGACAGGATTAGGTCCTGATGCTGGTGCTCCTTTGTCAGCACACCCAGATGTTGACAAGGTCGCTTTTACTGGGAGTTTTGAAACTGGTAAGAAGATTATGGCGGCTGCAGCTCCTATGGTTAAGCCTGTTACACTGGAACTTGGTGGAAAAAGTCCTATAGTAGTATTTGATGATGTTGACATTGACAAAGCTGTTGAGTGGACTCTGTTTGGGTGCTTTTGGACCAATGGTCAGATTTGCAGCGCAACATCTCGTCTTCTTGTCCATACAAAAATTGCTAAAGAATTTAATGAGAAGATGGTTGCATGGGCCAAGAATATTAAGGTTTCTGATCCGCTTGAAGAGGGTTGCAGACTTGGGCCAGTTGTTAGTGAAGGGCAGTATGAGAAGATTAAAAAGTTCATATTGAATGCCAAAAGCGAAGGTGCTACTATTCTGACTGGAGGTGTTAGACCTGCGCATCTTGAGAAGGGGTTCTTTATTGAACCAACAATCATTACTGATATCACCACATCAATGGAAATTTGGAGGGAGGAAGTCTTCGGTCCAGTCCTGTGTGTTAAAGAATTTAGCACTGAAGATGAAGCCATTGAACTGGCCAATGATACACAGTATGGTTTAGCTGGTGCTGTAATTTCTGGTGATCGTGAGCGCTGCCAGAGATTATCTGAGGAGATTGATGCTGGAATTATCTGGGTAAACTGCTCACAGCCCTGCTTCTGCCAGGCTCCCTGGGGCGGTAACAAGCGTAGTGGATTTGGACGTGAGCTTGGAGAAGGGGGCATTGATAACTACCTGAGCGTCAAGCAAGTCACGGAGTACATCTCTGATGAGCCGTGGGGATGGTATCGATCCCCCTCCAAGCTGTAAACTGGCATGAAATTTTGTTTGTTCCATTAGAATAAATATATCTCGATGCTATACAGAACGAACCCGTGTATCAAATGGAGTATGACATTGTGAAAAAGGCACATCAGCGGAACAAATGTCAGAGTTGACAATTCCCTCTAATGCAAATAGTTAACTCGCCCTTGAGTACCACTCCCTTGATCCC - >Zm00001eb041930_T001
ATAGCCTACACTACACAGGGCCGCAGCTTGCCGATCCCTAGCGACGAAGTCCACTGCCGAGTTGCCGACTCACCGACTCCCCTCGCAAGTCGCAATCTCCACTCTCCAGAGTCCAAACTCCACTCCTCACCGATGATGGCCTCGCAAGCGATGGTACCGCTGCGGCAGCTCTTTGTCGACGGCGAGTGGCGCCCGCCCGCGCAGGGCCGCCGCCTCCCCGTCGTCAACCCTACCACTGAGGCTCACATCGGCGAGATCCCGGCGGGCACGGCGGAGGACGTGGACGCCGCGGTGGCGGCGGCGCGCGCGGCGCTCAAGAGGAACCGCGGCCGCGATTGGGCGCGCGCGCCGGGGGCCGTTCGGGCCAAGTACCTCCGCGCCATCGCCGCCAAGGTAATTGAGAGGAAACAAGAGCTAGCTAAGCTAGAGGCACTTGATTGTGGGAAGCCTTATGATGAAGCTGCATGGGACATGGATGATGTTGCTGGGTGCTTTGAGTACTTCGCAGATCAGGCAGAAGCTTTGGACAAAAGACAAAATTCCCCAGTTTCTCTTCCGATGGAAACTTTTAAATGCCATCTCCGAAGAGAGCCTATTGGGGTAGTTGGGCTGATAACTCCTTGGAACTATCCTCTCCTGATGGCTACATGGAAGGTAGCTCCTGCTCTGGCTGCTGGTTGTGCAGCTGTGCTAAAGCCATCTGAATTGGCTTCTGTGACTTGCTTAGAGCTTGCTGATATCTGTAAAGAAGTCGGTCTTCCTCCCGGTGTCTTAAACATTGTGACAGGATTAGGTCCTGATGCTGGTGCTCCTTTGTCAGCACACCCAGATGTTGACAAGGTCGCTTTTACTGGGAGTTTTGAAACTGGTAAGAAGATTATGGCGGCTGCAGCTCCTATGGTTAAGCCTGTTACACTGGAACTTGGTGGAAAAAGTCCTATAGTAGTATTTGATGATGTTGACATTGACAAAGCTGTTGAGTGGACTCTGTTTGGGTGCTTTTGGACCAATGGTCAGATTTGCAGCGCAACATCTCGTCTTCTTGTCCATACAAAAATTGCTAAAGAATTTAATGAGAAGATGGTTGCATGGGCCAAGAATATTAAGGTTTCTGATCCGCTTGAAGAGGGTTGCAGACTTGGGCCAGTTGTTAGTGAAGGGCAGTATGAGAAGATTAAAAAGTTCATATTGAATGCCAAAAGCGAAGGTGCTACTATTCTGACTGGAGGTGTTAGACCTGCGCATCTTGAGAAGGGGTTCTTTATTGAACCAACAATCATTACTGATATCACCACATCAATGGAAATTTGGAGGGAGGAAGTCTTCGGTCCAGTCCTGTGTGTTAAAGAATTTAGCACTGAAGATGAAGCCATTGAACTGGCCAATGATACACAGTATGGTTTAGCTGGTGCTGTAATTTCTGGTGATCGTGAGCGCTGCCAGAGATTATCTGAGGAGATTGATGCTGGAATTATCTGGGTAAACTGCTCACAGCCCTGCTTCTGCCAGGCTCCCTGGGGCGGTAACAAGCGTAGTGGATTTGGACGTGAGCTTGGAGAAGGGGGCATTGATAACTACCTGAGCGTCAAGCAAGTCACGGAGTACATCTCTGATGAGCCGTGGGGATGGTATCGATCCCCCTCCAAGCTGTAAACTGGCATGAAATTTTGTTTGTTCCATTAGAATAAATATATCTCGATGCTATACAGAACGAACCCGTGTATCAAATGGAGTATGACATTGTGAAAAAGGCACATCAGCGGAACAAATGTCAGAGTTGACAATTCCCTCTAATGCAAATAGTTAACTCGCCCTTGAGTACCACTCCCTTGATCCC
CDS Sequence
- >Zm00001eb041930_T002
ATGGCCTCGCAAGCGATGGTACCGCTGCGGCAGCTCTTTGTCGACGGCGAGTGGCGCCCGCCCGCGCAGGGCCGCCGCCTCCCCGTCGTCAACCCTACCACTGAGGCTCACATCGGCGAGATCCCGGCGGGCACGGCGGAGGACGTGGACGCCGCGGTGGCGGCGGCGCGCGCGGCGCTCAAGAGGAACCGCGGCCGCGATTGGGCGCGCGCGCCGGGGGCCGTTCGGGCCAAGTACCTCCGCGCCATCGCCGCCAAGGTAATTGAGAGGAAACAAGAGCTAGCTAAGCTAGAGGCACTTGATTGTGGGAAGCCTTATGATGAAGCTGCATGGGACATGGATGATGTTGCTGGGTGCTTTGAGTACTTCGCAGATCAGGCAGAAGCTTTGGACAAAAGACAAAATTCCCCAGTTTCTCTTCCGATGGAAACTTTTAAATGCCATCTCCGAAGAGAGCCTATTGGGGTAGTTGGGCTGATAACTCCTTGGAACTATCCTCTCCTGATGGCTACATGGAAGGTAGCTCCTGCTCTGGCTGCTGGTTGTGCAGCTGTGCTAAAGCCATCTGAATTGGCTTCTGTGACTTGCTTAGAGCTTGCTGATATCTGTAAAGAAGTCGGTCTTCCTCCCGGTGTCTTAAACATTGTGACAGGATTAGGTCCTGATGCTGGTGCTCCTTTGTCAGCACACCCAGATGTTGACAAGGTCGCTTTTACTGGGAGTTTTGAAACTGGTAAGAAGATTATGGCGGCTGCAGCTCCTATGGTTAAGCCTGTTACACTGGAACTTGGTGGAAAAAGTCCTATAGTAGTATTTGATGATGTTGACATTGACAAAGCTGTTGAGTGGACTCTGTTTGGGTGCTTTTGGACCAATGGTCAGATTTGCAGCGCAACATCTCGTCTTCTTGTCCATACAAAAATTGCTAAAGAATTTAATGAGAAGATGGTTGCATGGGCCAAGAATATTAAGGTTTCTGATCCGCTTGAAGAGGGTTGCAGACTTGGGCCAGTTGTTAGTGAAGGGCAGTATGAGAAGATTAAAAAGTTCATATTGAATGCCAAAAGCGAAGGTGCTACTATTCTGACTGGAGGTGTTAGACCTGCGCATCTTGAGAAGGGGTTCTTTATTGAACCAACAATCATTACTGATATCACCACATCAATGGAAATTTGGAGGGAGGAAGTCTTCGGTCCAGTCCTGTGTGTTAAAGAATTTAGCACTGAAGATGAAGCCATTGAACTGGCCAATGATACACAGTATGGTTTAGCTGGTGCTGTAATTTCTGGTGATCGTGAGCGCTGCCAGAGATTATCTGAGGAGATTGATGCTGGAATTATCTGGGTAAACTGCTCACAGCCCTGCTTCTGCCAGGCTCCCTGGGGCGGTAACAAGCGTAGTGGATTTGGACGTGAGCTTGGAGAAGGGGGCATTGATAACTACCTGAGCGTCAAGCAAGTCACGGAGTACATCTCTGATGAGCCGTGGGGATGGTATCGATCCCCCTCCAAGCTGTAA - >Zm00001eb041930_T001
ATGGCCTCGCAAGCGATGGTACCGCTGCGGCAGCTCTTTGTCGACGGCGAGTGGCGCCCGCCCGCGCAGGGCCGCCGCCTCCCCGTCGTCAACCCTACCACTGAGGCTCACATCGGCGAGATCCCGGCGGGCACGGCGGAGGACGTGGACGCCGCGGTGGCGGCGGCGCGCGCGGCGCTCAAGAGGAACCGCGGCCGCGATTGGGCGCGCGCGCCGGGGGCCGTTCGGGCCAAGTACCTCCGCGCCATCGCCGCCAAGGTAATTGAGAGGAAACAAGAGCTAGCTAAGCTAGAGGCACTTGATTGTGGGAAGCCTTATGATGAAGCTGCATGGGACATGGATGATGTTGCTGGGTGCTTTGAGTACTTCGCAGATCAGGCAGAAGCTTTGGACAAAAGACAAAATTCCCCAGTTTCTCTTCCGATGGAAACTTTTAAATGCCATCTCCGAAGAGAGCCTATTGGGGTAGTTGGGCTGATAACTCCTTGGAACTATCCTCTCCTGATGGCTACATGGAAGGTAGCTCCTGCTCTGGCTGCTGGTTGTGCAGCTGTGCTAAAGCCATCTGAATTGGCTTCTGTGACTTGCTTAGAGCTTGCTGATATCTGTAAAGAAGTCGGTCTTCCTCCCGGTGTCTTAAACATTGTGACAGGATTAGGTCCTGATGCTGGTGCTCCTTTGTCAGCACACCCAGATGTTGACAAGGTCGCTTTTACTGGGAGTTTTGAAACTGGTAAGAAGATTATGGCGGCTGCAGCTCCTATGGTTAAGCCTGTTACACTGGAACTTGGTGGAAAAAGTCCTATAGTAGTATTTGATGATGTTGACATTGACAAAGCTGTTGAGTGGACTCTGTTTGGGTGCTTTTGGACCAATGGTCAGATTTGCAGCGCAACATCTCGTCTTCTTGTCCATACAAAAATTGCTAAAGAATTTAATGAGAAGATGGTTGCATGGGCCAAGAATATTAAGGTTTCTGATCCGCTTGAAGAGGGTTGCAGACTTGGGCCAGTTGTTAGTGAAGGGCAGTATGAGAAGATTAAAAAGTTCATATTGAATGCCAAAAGCGAAGGTGCTACTATTCTGACTGGAGGTGTTAGACCTGCGCATCTTGAGAAGGGGTTCTTTATTGAACCAACAATCATTACTGATATCACCACATCAATGGAAATTTGGAGGGAGGAAGTCTTCGGTCCAGTCCTGTGTGTTAAAGAATTTAGCACTGAAGATGAAGCCATTGAACTGGCCAATGATACACAGTATGGTTTAGCTGGTGCTGTAATTTCTGGTGATCGTGAGCGCTGCCAGAGATTATCTGAGGAGATTGATGCTGGAATTATCTGGGTAAACTGCTCACAGCCCTGCTTCTGCCAGGCTCCCTGGGGCGGTAACAAGCGTAGTGGATTTGGACGTGAGCTTGGAGAAGGGGGCATTGATAACTACCTGAGCGTCAAGCAAGTCACGGAGTACATCTCTGATGAGCCGTGGGGATGGTATCGATCCCCCTCCAAGCTGTAA
Protein Sequence
- >Zm00001eb041930_P002
MASQAMVPLRQLFVDGEWRPPAQGRRLPVVNPTTEAHIGEIPAGTAEDVDAAVAAARAALKRNRGRDWARAPGAVRAKYLRAIAAKVIERKQELAKLEALDCGKPYDEAAWDMDDVAGCFEYFADQAEALDKRQNSPVSLPMETFKCHLRREPIGVVGLITPWNYPLLMATWKVAPALAAGCAAVLKPSELASVTCLELADICKEVGLPPGVLNIVTGLGPDAGAPLSAHPDVDKVAFTGSFETGKKIMAAAAPMVKPVTLELGGKSPIVVFDDVDIDKAVEWTLFGCFWTNGQICSATSRLLVHTKIAKEFNEKMVAWAKNIKVSDPLEEGCRLGPVVSEGQYEKIKKFILNAKSEGATILTGGVRPAHLEKGFFIEPTIITDITTSMEIWREEVFGPVLCVKEFSTEDEAIELANDTQYGLAGAVISGDRERCQRLSEEIDAGIIWVNCSQPCFCQAPWGGNKRSGFGRELGEGGIDNYLSVKQVTEYISDEPWGWYRSPSKL - >Zm00001eb041930_P001
MASQAMVPLRQLFVDGEWRPPAQGRRLPVVNPTTEAHIGEIPAGTAEDVDAAVAAARAALKRNRGRDWARAPGAVRAKYLRAIAAKVIERKQELAKLEALDCGKPYDEAAWDMDDVAGCFEYFADQAEALDKRQNSPVSLPMETFKCHLRREPIGVVGLITPWNYPLLMATWKVAPALAAGCAAVLKPSELASVTCLELADICKEVGLPPGVLNIVTGLGPDAGAPLSAHPDVDKVAFTGSFETGKKIMAAAAPMVKPVTLELGGKSPIVVFDDVDIDKAVEWTLFGCFWTNGQICSATSRLLVHTKIAKEFNEKMVAWAKNIKVSDPLEEGCRLGPVVSEGQYEKIKKFILNAKSEGATILTGGVRPAHLEKGFFIEPTIITDITTSMEIWREEVFGPVLCVKEFSTEDEAIELANDTQYGLAGAVISGDRERCQRLSEEIDAGIIWVNCSQPCFCQAPWGGNKRSGFGRELGEGGIDNYLSVKQVTEYISDEPWGWYRSPSKL
Source: Genetic information comes from the article