Gene Details:
- Gene ID: Zm00001eb013080
- Gene Symbol: ZmAPX1 apx3
- Gene Name: ascorbate peroxidase 1
- Genome: Zm-B73-REFERENCE-NAM-5.0
- Species: Zea mays
Functional Descriptions:
- These proteins included an ascorbate peroxidase (Zea mays ascorbate peroxidase 1 (ZmAPX1)) encoded by a gene located within the mapping interval of a previously identified quantitative trait locus associated with SCLB resistance.
- ZmAPX1 overexpression resulted in lower H2O2 accumulation and enhanced resistance against B. maydis. Jasmonic acid (JA) contents and transcript levels for JA biosynthesis and responsive genes increased in ZmAPX1-overexpressing plants infected with B. maydis, whereas ZmAPX1 mutants showed the opposite effects.
- These results demonstrate that ZmAPX1 positively regulates SCLB resistance by decreasing H2O2 accumulation and activating the JA-mediated defense signaling pathway. This study identified ZmAPX1 as a potentially useful gene for increasing SCLB resistance. Furthermore, the generated data may be relevant for clarifying the functions of plant APXs.
- Overexpression of Zea mays ascorbate peroxidase 1 (ZmAPX1) confers resistance against southern corn leaf blight.
- These results suggest that ZmAPX1 decreases the accumulation of H2O2. In addition, we measured H2O2 contents in B. maydis-infected Mo17 and B73 plants, finding that the Mo17 plants accumulated lower H2O2 levels than B73 plants.
- Taken together, these results suggest that the enhanced resistance against SCLB conferred by ZmAPX1 might be mediated by JA signaling.
Function-related keywords:
- leaf , resistance , ja , blight , defense , jasmonic , jasmonic-acid , signaling-pathway-gene , ja-biosynthesis , ja-signaling , plant-defense-signaling
Literature:
- Ascorbate peroxidase 1 confers resistance to southern corn leaf blight in maize. DOI: 10.1111/jipb.13254 ; PMID: 35319160
Related News:
Gene Resources:
- NCBI ID: LOC100285887
- UniProt accessions: B6U9S6
Sequences:
cDNA Sequence
- >Zm00001eb013080_T002
AGATTGACGGCCATGATGCACCCTACGAGAAGCTTCCCTTTCTCCTTGTCCTTGCAACCCTCCGCCCTTTCGGTTGTCGCAATATAAACTTGCCGGGGAGCGTGGCGACCATTTGCCCCCAGCAGATCTTGTGACCCTCCCTCAGCCGCGTCGCGTCGCATCCTACGATCCAAAGCTCTCTCTGGTCGCAGGTCGCAGCCATGGCGAAGAACTACCCGACCGTGAGCGCCGAGTACAGCGAGGCTGTGGACAAGGCCAGGCGCAAGCTCCGAGCCCTCATCGCCGAGAAGAGCTGCGCCCCGCTCATGCTCCGCCTCGCGTGGCACTCCGCGGGGACGTTCGACGTGTCGTCGAGGACCGGCGGTCCATTCGGCACGATGAAGCATCAGTCGGAATTGGCTCACGGCGCTAACGCGGGGCTGGACATCGCGGTGCGGCTGCTCGAGCCCATCAAGGAGGAGTTCCCAATCCTCTCTTACGCCGATTTCTACCAGCTCGCGGGAGTTGTGGCCGTGGAGGTCACCGGTGGGCCTGAGATTCCCTTCCACCCCGGTAGGGAGGACAAGCCTCAGCCCCCACCTGAGGGCCGCCTTCCTGATGCCACTAAGGGTTCTGACCACCTGAGGCAAGTTTTTGGCAAGCAGATGGGCTTGAGCGATCAGGACATTGTTGCCCTCTCTGGTGGCCACACCTTGGGAAGGTGCCACAAAGAGCGGTCTGGTTTCGAGGGGGCCTGGACTACAAACCCTTTGGTCTTTGACAACTCTTACTTCAAGGAACTTCTGAGTGGTGATAAGGAGGGCCTTCTTCAGCTCCCAAGTGACAAAGCCCTGCTGAGTGACCCTGTCTTCCGCCCTCTTGTCGAGAAATATGCTGCGGATGAGAAGGCTTTCTTTGATGACTACAAAGAGGCCCACCTCAAGCTCTCCGAACTGGGGTTTGCTGATGCTTAAATAGACCCTATCCTGGAGTGATACATTCTGCTGCATGTGGTCTTTTGCATCTGGAGTCAATGTGAACAAGCAGATTGTCGTATTGTCTTTCTCGTAATAAATTTGTCAATGTTGAGCCCTTAGGCTTGAATTGTGGGACCCTTTGTTCGTTTTCCTAGACTCTGATGCTGTATGCAACTGAAACGAGTAAATCTATGATCTTAAGGCTGCAGTATGTTCTTGTGTTACTGTGCGTTTATCTAGTTTCCGTTGGTTCGGTTCACAGCTTGTGTGGTTTAATAGGTTGTGCTTGTGCTGCGTGACTGGTCGTGCAACTTGAGTCCGAGTCTTCCATAGTTCCAATTTTCTCTTGGCGGCT - >Zm00001eb013080_T001
AGATTGACGGCCATGATGCACCCTACGAGAAGCTTCCCTTTCTCCTTGTCCTTGCAACCCTCCGCCCTTTCGGTTGTCGCAATATAAACTTGCCGGGGAGCGTGGCGACCATTTGCCCCCAGCAGATCTTGTGACCCTCCCTCAGCCGCGTCGCGTCGCATCCTACGATCCAAAGCTCTCTCTGGTCGCAGGTCGCAGCCATGGCGAAGAACTACCCGACCGTGAGCGCCGAGTACAGCGAGGCTGTGGACAAGGCCAGGCGCAAGCTCCGAGCCCTCATCGCCGAGAAGAGCTGCGCCCCGCTCATGCTCCGCCTCGCGTGGCACTCCGCGGGGACGTTCGACGTGTCGTCGAGGACCGGCGGTCCATTCGGCACGATGAAGCATCAGTCGGAATTGGCTCACGGCGCTAACGCGGGGCTGGACATCGCGGTGCGGCTGCTCGAGCCCATCAAGGAGGAGTTCCCAATCCTCTCTTACGCCGATTTCTACCAGCTCGCGGGAGTTGTGGCCGTGGAGGTCACCGGTGGGCCTGAGATTCCCTTCCACCCCGGTAGGGAGGACAAGCCTCAGCCCCCACCTGAGGGCCGCCTTCCTGATGCCACTAAGGGTTCTGACCACCTGAGGCAAGTTTTTGGCAAGCAGATGGGCTTGAGCGATCAGGACATTGTTGCCCTCTCTGGTGGCCACACCTTGGGAAGGTGCCACAAAGAGCGGTCTGGTTTCGAGGGGGCCTGGACTACAAACCCTTTGGTCTTTGACAACTCTTACTTCAAGGAACTTCTGAGTGGTGATAAGGAGGGCCTTCTTCAGCTCCCAAGTGACAAAGCCCTGCTGAGTGACCCTGTCTTCCGCCCTCTTGTCGAGAAATATGCTGCGGATGAGAAGGCTTTCTTTGATGACTACAAAGAGGCCCACCTCAAGCTCTCCGAACTGGGGTTTGCTGATGCTTAAATAGACCCTATCCTGGAGTGATACATTCTGCTGCATGTGGTCTTTTGCATCTGGAGTCAATGTGAACAAGCAGATTGTCGTATTGTCTTTCTCGTAATAAATTTGTCAATGTTGAGCCCTTAGGCTTGAATTGTGGGACCCTTTGTTCGTTTTCCTAGACTCTGATGCTGTATGCAACTGAAACGAGTAAATCTATGATCTTAAGGCTGCAGTATGTTCTTGTGTTACTGTGCGTTTATCTAGTTTCCGTTGGTTCGGTTCACAGCTTGTGTGGTTTAATAGGTTGTGCTTGTGCTGCGTGACTGGTCGTGCAACTTGAGTCCGAGTCTTCCATAGTTCCAATTTTCTCTTGGCGGCT
CDS Sequence
- >Zm00001eb013080_T002
ATGGCGAAGAACTACCCGACCGTGAGCGCCGAGTACAGCGAGGCTGTGGACAAGGCCAGGCGCAAGCTCCGAGCCCTCATCGCCGAGAAGAGCTGCGCCCCGCTCATGCTCCGCCTCGCGTGGCACTCCGCGGGGACGTTCGACGTGTCGTCGAGGACCGGCGGTCCATTCGGCACGATGAAGCATCAGTCGGAATTGGCTCACGGCGCTAACGCGGGGCTGGACATCGCGGTGCGGCTGCTCGAGCCCATCAAGGAGGAGTTCCCAATCCTCTCTTACGCCGATTTCTACCAGCTCGCGGGAGTTGTGGCCGTGGAGGTCACCGGTGGGCCTGAGATTCCCTTCCACCCCGGTAGGGAGGACAAGCCTCAGCCCCCACCTGAGGGCCGCCTTCCTGATGCCACTAAGGGTTCTGACCACCTGAGGCAAGTTTTTGGCAAGCAGATGGGCTTGAGCGATCAGGACATTGTTGCCCTCTCTGGTGGCCACACCTTGGGAAGGTGCCACAAAGAGCGGTCTGGTTTCGAGGGGGCCTGGACTACAAACCCTTTGGTCTTTGACAACTCTTACTTCAAGGAACTTCTGAGTGGTGATAAGGAGGGCCTTCTTCAGCTCCCAAGTGACAAAGCCCTGCTGAGTGACCCTGTCTTCCGCCCTCTTGTCGAGAAATATGCTGCGGATGAGAAGGCTTTCTTTGATGACTACAAAGAGGCCCACCTCAAGCTCTCCGAACTGGGGTTTGCTGATGCTTAA - >Zm00001eb013080_T001
ATGGCGAAGAACTACCCGACCGTGAGCGCCGAGTACAGCGAGGCTGTGGACAAGGCCAGGCGCAAGCTCCGAGCCCTCATCGCCGAGAAGAGCTGCGCCCCGCTCATGCTCCGCCTCGCGTGGCACTCCGCGGGGACGTTCGACGTGTCGTCGAGGACCGGCGGTCCATTCGGCACGATGAAGCATCAGTCGGAATTGGCTCACGGCGCTAACGCGGGGCTGGACATCGCGGTGCGGCTGCTCGAGCCCATCAAGGAGGAGTTCCCAATCCTCTCTTACGCCGATTTCTACCAGCTCGCGGGAGTTGTGGCCGTGGAGGTCACCGGTGGGCCTGAGATTCCCTTCCACCCCGGTAGGGAGGACAAGCCTCAGCCCCCACCTGAGGGCCGCCTTCCTGATGCCACTAAGGGTTCTGACCACCTGAGGCAAGTTTTTGGCAAGCAGATGGGCTTGAGCGATCAGGACATTGTTGCCCTCTCTGGTGGCCACACCTTGGGAAGGTGCCACAAAGAGCGGTCTGGTTTCGAGGGGGCCTGGACTACAAACCCTTTGGTCTTTGACAACTCTTACTTCAAGGAACTTCTGAGTGGTGATAAGGAGGGCCTTCTTCAGCTCCCAAGTGACAAAGCCCTGCTGAGTGACCCTGTCTTCCGCCCTCTTGTCGAGAAATATGCTGCGGATGAGAAGGCTTTCTTTGATGACTACAAAGAGGCCCACCTCAAGCTCTCCGAACTGGGGTTTGCTGATGCTTAA
Protein Sequence
- >Zm00001eb013080_P002
MAKNYPTVSAEYSEAVDKARRKLRALIAEKSCAPLMLRLAWHSAGTFDVSSRTGGPFGTMKHQSELAHGANAGLDIAVRLLEPIKEEFPILSYADFYQLAGVVAVEVTGGPEIPFHPGREDKPQPPPEGRLPDATKGSDHLRQVFGKQMGLSDQDIVALSGGHTLGRCHKERSGFEGAWTTNPLVFDNSYFKELLSGDKEGLLQLPSDKALLSDPVFRPLVEKYAADEKAFFDDYKEAHLKLSELGFADA - >Zm00001eb013080_P001
MAKNYPTVSAEYSEAVDKARRKLRALIAEKSCAPLMLRLAWHSAGTFDVSSRTGGPFGTMKHQSELAHGANAGLDIAVRLLEPIKEEFPILSYADFYQLAGVVAVEVTGGPEIPFHPGREDKPQPPPEGRLPDATKGSDHLRQVFGKQMGLSDQDIVALSGGHTLGRCHKERSGFEGAWTTNPLVFDNSYFKELLSGDKEGLLQLPSDKALLSDPVFRPLVEKYAADEKAFFDDYKEAHLKLSELGFADA
Source: Genetic information comes from the article