Gene Details:

Functional Descriptions:

  • Two root epidermis-expressed AMT1-type AMTs, ZmAMT1.1a and ZmAMT1;3, mediate uptake, representing the major components of the Root-N pathway for ammonium uptake in maize.
  • Furthermore, AMFs downregulated ZmAMT1.1a and ZmAMT1;3 protein abundance and transport activities expressed in the root epidermis, suggesting a trade-off between mycorrhizal and direct root N-uptake pathways.
  • After mycorrhizal inoculation, the transcripts of ZmAMT1.1a and ZmAMT1;3 were unchanged, but, interestingly, the abundances of ZmAMT1.1a and ZmAMT1;3 proteins were significantly downregulated to half their levels.
  • Interestingly, with AMF inoculation, the expression of epidermis-expressed AMTs ZmAMT1.1a and ZmAMT1;3 for the Root-N pathway were suppressed at the protein level, but not at the transcriptional level.
  • Additionally, phosphorylation modifications occurred at the conserved C-terminal region of the ZmAMT1.1a and ZmAMT1;3 proteins, potentially inactivating transporters responsible for the Root-N uptake pathway.
  • In mycorrhizal roots, ZmAMT3;1 transcripts and proteins are upregulated, thus inducing the Myc-N pathway, while the protein abundance and activity of the epidermis-expressed ZmAMT1.1a/ZmAMT1;3 responsible for Root-N pathway are suppressed. Under greenhouse and field conditions, the ZmAMT3;1-mediated Myc-N pathway contributes significantly to plant overall N acquisition.
  • The two rhizodermis-localized transporters ZmAMT1;1a and ZmAMT1;3 are most probably the major components in the high-affinity transport system in maize roots. A particular regulatory feature is their persistent induction by ammonium rather than an up-regulation under N deficiency.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Zm00001eb060210_T001
    ATGTCGACGGGCGCAAACACGGAAGCCGCCGCCAGCAGCAGCCCGAGCCCAGGCAATAAGCGGTGCGCCGCGTGCAAGAACCAGCGCAGGAAGTGCGGCCAGGGCTGCGCCCTGGCCCCCTACTTCCCGGCATCGGATCCCCAGAGATACGCCTGCGTGCAGAGGGTCTTCGGCGCCAGCAACATTGCAAGGATGCTCCAGAATCTCCCTGTCCACGAACGAGCCAGAGCGGCGGACACGATGGCGGTGGAGGCACGCCGGCGCGTGCAGGACCCGGTGTACGGCTGCGCTGGGGTCGTGGGCCACCTCCAGGGAGAGATCAGAGCGACGCAGTGCGAGCTGGCCAGGACCCAGGCCCAGATCGCCGTGCACGCCGCGGTAGCTCGCGCGCACCCGGCGCCGGGCCCTCTGCAGCAGCAAGAGGAGGAGGAGGAGGACGATGATGCGCGGGAGCCGTTCCAAGGATTGGATGCCTATGCCCTGGTTCCTCGATGA
CDS Sequence
  • >Zm00001eb060210_T001
    ATGTCGACGGGCGCAAACACGGAAGCCGCCGCCAGCAGCAGCCCGAGCCCAGGCAATAAGCGGTGCGCCGCGTGCAAGAACCAGCGCAGGAAGTGCGGCCAGGGCTGCGCCCTGGCCCCCTACTTCCCGGCATCGGATCCCCAGAGATACGCCTGCGTGCAGAGGGTCTTCGGCGCCAGCAACATTGCAAGGATGCTCCAGAATCTCCCTGTCCACGAACGAGCCAGAGCGGCGGACACGATGGCGGTGGAGGCACGCCGGCGCGTGCAGGACCCGGTGTACGGCTGCGCTGGGGTCGTGGGCCACCTCCAGGGAGAGATCAGAGCGACGCAGTGCGAGCTGGCCAGGACCCAGGCCCAGATCGCCGTGCACGCCGCGGTAGCTCGCGCGCACCCGGCGCCGGGCCCTCTGCAGCAGCAAGAGGAGGAGGAGGAGGACGATGATGCGCGGGAGCCGTTCCAAGGATTGGATGCCTATGCCCTGGTTCCTCGATGA
Protein Sequence
  • >Zm00001eb060210_P001
    MSTGANTEAAASSSPSPGNKRCAACKNQRRKCGQGCALAPYFPASDPQRYACVQRVFGASNIARMLQNLPVHERARAADTMAVEARRRVQDPVYGCAGVVGHLQGEIRATQCELARTQAQIAVHAAVARAHPAPGPLQQQEEEEEDDDAREPFQGLDAYALVPR

Source: Genetic information comes from the article