Gene Details:

Functional Descriptions:

  • The maize high-lysine mutant O7|opaque7 is defective in an acyl-CoA synthetase-like protein.
  • The maize classic mutant O7|opaque7 (O7|opaque7) was initially cloned for its high lysine trait, although the gene function was not understood until its homolog in Arabidopsis thaliana was found to encode an oxalyl-CoA synthetase (EC 6.2.1.8), which ligates oxalate and CoA to form oxalyl-CoA.
  • Our enzymatic analysis showed that ZmOCD1 catalyzes oxalyl-CoA, the product of O7|opaque7, into formyl-CoA and CO2 for degradation. Mutations in ocd1 caused dramatic alterations in the metabolome in the endosperm.
  • Our findings demonstrate that ZmOCD1 acts downstream of O7|opaque7 in oxalate degradation and affects endosperm development, the metabolome, and nutritional quality in maize seeds.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Zm00001eb434030_T002
    GAAAAACCTTTGCGTTTGGTCCACTGTTTGCAGGCGTATGTGCAGGTTGCAACACAGCGGCAGCCAAAGTTGATTTGGTTTCTTGCCCGGAAATCCGACACGCCGCCGCGATCGCCATCACCTAACCGTCGCTGACGAGGCAACTCGCCGGCCATATAAATACGCACCGCCTGGCGCCTGCCACGCCACGGGCAGCTCAGCTCAGCCAGCCGCACAAGCCCCAAACAGAAAGAGACTCACCCACCGCGCCACGCAACAGCCCATCTCCGCTCTGCTCTCTCGCCCACCCCGATTCCCCCCCATGGCTATGGCGACGACGGAGGCCACCACGCTGACGGCGCTGCTCAAGGAGGCGGCGGCCGCCTTCCCGACCCGCCGCGCAGTTGCCGTACCGGGCAGGCTGGAGCTCACGCACGCCGCCCTCGACGCTCTCGTCGACGCGGCGGCCGCACGACTCGCCGCCGACGCCGGAGTTCTCCCGGGCCACGTCGTCGCGCTTTCCTTCCCCAACACCGTCGAGCTGGTGATCATGTTCCTGGCGGTAATCCGCGCGCGCGGCGTGGCGGCGCCGCTCAACCCGGCCTACACGCAGGAGGAGTTCGAGTTCTACCTCTCCGACTCGGAGGCGCGCCTCCTCGTCACCAACGCGGAGGGCAACGCGGCGGCGCAGGCGGCCGCTGCCAAGCTCGGCCTCGCCCACGCCGCCGCCAGCCTCCACGACGCGGCCGGCCCTGTCCACCTCGCCGGCCTCCCGGCCCATGCTCCGGAGAACGGGTCCCACGGCGGCGGCGCGGCGGGCTCTCTCTCCCCCAACGACCCGTCGGACGTGGCCCTGTTCCTGCACACCTCCGGCACGACGAGCCGGCCCAAGGGCGTGCCGCTGAAGCAGCGCAACCTGGCGGCGTCGGTGCGGAACATCCGGTTCTCGGCGTCCACGTTCTGGGCCGACATGCGGGCGTCGGGCGCCACCTGGTACACGGCGGTGCCGACGATCCACCAGATCATCCTGGACCGGCACGCGTCGCGGCCGGAGGCGTACCCGGCGCTCCGATTCATCCGCAGCTGCAGCGCGTCGCTGGCGCCGGCGATCCTGGAGCGGCTGGAGGCGGCGTTCAGCGCGCCGGTGCTGGAGGCGTACGCGATGACGGAGGCGTCGCACCTGATGACGTCAAACCCGCTGCCGGAGGACGGGCCACGGAAGCCCGGGTCAGTGGGGCGCGCGGTGGGGCAGGAGCTGGCGGTGCTGGACGAGGAGGGGCGGCTCGTGGCGGCGGGGAGCCCCGGGGAGGTGTGCGTCCGCGGGGACAACGTGACGGCGGGTTACAAGGGGAACCCAGAGGCGAACGAGGCGGCGTTCCGGTTCGGGTGGTTCCACACGGGGGACATCGGCGTGGTGGATGACCAAGGGTACGTCCGGCTGGTGGGGCGCATCAAGGAGCTCATCAACCGCGGCGGGGAGAAGATCTCCCCGATCGAGGTGGACGCCGTGCTGCTGGGCCTCCCCGGTGTGGCGCAGGCGGTGTCGTTCGGGGTGCCTGACGACAAGTACGGGGAGGAGATCAACTGTGCGGTGATCCCGCGGGACGGGTCGGCGCTGCGGGAGGAGGAGGTGCTGGCGCACTGCCGGCGGAACCTGGCGTCGTTCAAGGTGCCCAAGAAGGTGTTCATCACGGACGACCTGCCCAAGACGGCCACCGGAAAGATCCAGCGCCGCATCGTGGCGCAGCACTTCGTGCAGCCGACCAGTGCCTGATCGTTGATCCTGGTGCTTGCCAAGTCATGGGTCGACGGATTGGAATTTCGCTACACTAGCTAGCATTTTGCCGTTGTTCCCGCATTGCGAGGTGGACATGGCCTGACTGAACCTGAATAAACAAACACATGCATCGTCTCTCTCTCGTCGTCGTTCTTTGCGTTCCGCGTATGTGTCGGCTCATCTCCTGTCGTTTACCGGACGGCATGGTGCCAACAATCGGTGGGCAGGTGTCATCAGGCGTGCATTGTTTTTTTCTTTTTATTACCGGTTGATTTGGAACCGTAATTTAGGTTTATCGATTAGAAAAACGTTTGGGAAAAGTGCCACATGCCTTTGTTGAAAAGGACTTCTTTCTGTTTTTTTACCTTTTACTTTCTTTTTGAATCTGCTTTTTTACTATGTTTCCTATATTCACACAATCTATATTTGAAAATTGGCAACAAAGAAAATTAAAGGAAACAATTGAATCGATATTTTTAAAATAGAGTAACTTATCTTTGTATGTCGAAAAACTATGAACTGGATTCAAATATGTACATTTAGTATTTTGT
  • >Zm00001eb434030_T001
    GAAAAACCTTTGCGTTTGGTCCACTGTTTGCAGGCGTATGTGCAGGTTGCAACACAGCGGCAGCCAAAGTTGATTTGGTTTCTTGCCCGGAAATCCGACACGCCGCCGCGATCGCCATCACCTAACCGTCGCTGACGAGGCAACTCGCCGGCCATATAAATACGCACCGCCTGGCGCCTGCCACGCCACGGGCAGCTCAGCTCAGCCAGCCGCACAAGCCCCAAACAGAAAGAGACTCACCCACCGCGCCACGCAACAGCCCATCTCCGCTCTGCTCTCTCGCCCACCCCGATTCCCCCCCATGGCTATGGCGACGACGGAGGCCACCACGCTGACGGCGCTGCTCAAGGAGGCGGCGGCCGCCTTCCCGACCCGCCGCGCAGTTGCCGTACCGGGCAGGCTGGAGCTCACGCACGCCGCCCTCGACGCTCTCGTCGACGCGGCGGCCGCACGACTCGCCGCCGACGCCGGAGTTCTCCCGGGCCACGTCGTCGCGCTTTCCTTCCCCAACACCGTCGAGCTGGTGATCATGTTCCTGGCGGTAATCCGCGCGCGCGGCGTGGCGGCGCCGCTCAACCCGGCCTACACGCAGGAGGAGTTCGAGTTCTACCTCTCCGACTCGGAGGCGCGCCTCCTCGTCACCAACGCGGAGGGCAACGCGGCGGCGCAGGCGGCCGCTGCCAAGCTCGGCCTCGCCCACGCCGCCGCCAGCCTCCACGACGCGGCCGGCCCTGTCCACCTCGCCGGCCTCCCGGCCCATGCTCCGGAGAACGGGTCCCACGGCGGCGGCGCGGCGGGCTCTCTCTCCCCCAACGACCCGTCGGACGTGGCCCTGTTCCTGCACACCTCCGGCACGACGAGCCGGCCCAAGGGCGTGCCGCTGAAGCAGCGCAACCTGGCGGCGTCGGTGCGGAACATCCGGTTCTCGGCGTCCACGTTCTGGGCCGACATGCGGGCGTCGGGCGCCACCTGGTACACGGCGGTGCCGACGATCCACCAGATCATCCTGGACCGGCACGCGTCGCGGCCGGAGGCGTACCCGGCGCTCCGATTCATCCGCAGCTGCAGCGCGTCGCTGGCGCCGGCGATCCTGGAGCGGCTGGAGGCGGCGTTCAGCGCGCCGGTGCTGGAGGCGTACGCGATGACGGAGGCGTCGCACCTGATGACGTCAAACCCGCTGCCGGAGGACGGGCCACGGAAGCCCGGGTCAGTGGGGCGCGCGGTGGGGCAGGAGCTGGCGGTGCTGGACGAGGAGGGGCGGCTCGTGGCGGCGGGGAGCCCCGGGGAGGTGTGCGTCCGCGGGGACAACGTGACGGCGGGTTACAAGGGGAACCCAGAGGCGAACGAGGCGGCGTTCCGGTTCGGGTGGTTCCACACGGGGGACATCGGCGTGGTGGATGACCAAGGGTACGTCCGGCTGGTGGGGCGCATCAAGGAGCTCATCAACCGCGGCGGGGAGAAGATCTCCCCGATCGAGGTGGACGCCGTGCTGCTGGGCCTCCCCGGTGTGGCGCAGGCGGTGTCGTTCGGGGTGCCTGACGACAAGTACGGGGAGGAGATCAACTGTGCGGTGATCCCGCGGGACGGGTCGGCGCTGCGGGAGGAGGAGGTGCTGGCGCACTGCCGGCGGAACCTGGCGTCGTTCAAGGTGCCCAAGAAGGTGTTCATCACGGACGACCTGCCCAAGACGGCCACCGGAAAGATCCAGCGCCGCATCGTGGCGCAGCACTTCGTGCAGCCGACCAGTGCCTGATCGTTGATCCTGGTGCTTGCCAAGTCATGGGTCGACGGATTGGAATTTCGCTACACTAGCTAGCATTTTGCCGTTGTTCCCGCATTGCGAGGTGGACATGGCCTGACTGAACCTGAATAAACAAACACATGCATCGTCTCTCTCTCGTCGTCGTTCTTTGCGTTCCGCGTATGTGTCGGCTCATCTCCTGTCGTTTACCGGACGGCATGGTGCCAACAATCGGTGGGCAGGTGTCATCAGGCGTGCATTGTTTTTTTCTTTTTATTACCGGTTGATTTGGAACCGTAATTTAGGTTTATCGATTAGAAAAACGTTTGGGAAAAGTGCCACATGCCTTTGTTGAAAAGGACTTCTTTCTGTTTTTTTACCTTTTACTTTCTTTTTGAATCTGCTTTTTTACTATGTTTCCTATATTCACACAATCTATATTTGAAAATTGGCAACAAAGAAAATTAAAGGAAACAATTGAATCGATATTTTTAAAATAGAGTAACTTATCTTTGTATGTCGAAAAACTATGAACTGGATTCAAATATGTACATTTAGTATTTTGT
CDS Sequence
  • >Zm00001eb434030_T002
    ATGGCTATGGCGACGACGGAGGCCACCACGCTGACGGCGCTGCTCAAGGAGGCGGCGGCCGCCTTCCCGACCCGCCGCGCAGTTGCCGTACCGGGCAGGCTGGAGCTCACGCACGCCGCCCTCGACGCTCTCGTCGACGCGGCGGCCGCACGACTCGCCGCCGACGCCGGAGTTCTCCCGGGCCACGTCGTCGCGCTTTCCTTCCCCAACACCGTCGAGCTGGTGATCATGTTCCTGGCGGTAATCCGCGCGCGCGGCGTGGCGGCGCCGCTCAACCCGGCCTACACGCAGGAGGAGTTCGAGTTCTACCTCTCCGACTCGGAGGCGCGCCTCCTCGTCACCAACGCGGAGGGCAACGCGGCGGCGCAGGCGGCCGCTGCCAAGCTCGGCCTCGCCCACGCCGCCGCCAGCCTCCACGACGCGGCCGGCCCTGTCCACCTCGCCGGCCTCCCGGCCCATGCTCCGGAGAACGGGTCCCACGGCGGCGGCGCGGCGGGCTCTCTCTCCCCCAACGACCCGTCGGACGTGGCCCTGTTCCTGCACACCTCCGGCACGACGAGCCGGCCCAAGGGCGTGCCGCTGAAGCAGCGCAACCTGGCGGCGTCGGTGCGGAACATCCGGTTCTCGGCGTCCACGTTCTGGGCCGACATGCGGGCGTCGGGCGCCACCTGGTACACGGCGGTGCCGACGATCCACCAGATCATCCTGGACCGGCACGCGTCGCGGCCGGAGGCGTACCCGGCGCTCCGATTCATCCGCAGCTGCAGCGCGTCGCTGGCGCCGGCGATCCTGGAGCGGCTGGAGGCGGCGTTCAGCGCGCCGGTGCTGGAGGCGTACGCGATGACGGAGGCGTCGCACCTGATGACGTCAAACCCGCTGCCGGAGGACGGGCCACGGAAGCCCGGGTCAGTGGGGCGCGCGGTGGGGCAGGAGCTGGCGGTGCTGGACGAGGAGGGGCGGCTCGTGGCGGCGGGGAGCCCCGGGGAGGTGTGCGTCCGCGGGGACAACGTGACGGCGGGTTACAAGGGGAACCCAGAGGCGAACGAGGCGGCGTTCCGGTTCGGGTGGTTCCACACGGGGGACATCGGCGTGGTGGATGACCAAGGGTACGTCCGGCTGGTGGGGCGCATCAAGGAGCTCATCAACCGCGGCGGGGAGAAGATCTCCCCGATCGAGGTGGACGCCGTGCTGCTGGGCCTCCCCGGTGTGGCGCAGGCGGTGTCGTTCGGGGTGCCTGACGACAAGTACGGGGAGGAGATCAACTGTGCGGTGATCCCGCGGGACGGGTCGGCGCTGCGGGAGGAGGAGGTGCTGGCGCACTGCCGGCGGAACCTGGCGTCGTTCAAGGTGCCCAAGAAGGTGTTCATCACGGACGACCTGCCCAAGACGGCCACCGGAAAGATCCAGCGCCGCATCGTGGCGCAGCACTTCGTGCAGCCGACCAGTGCCTGA
  • >Zm00001eb434030_T001
    ATGGCTATGGCGACGACGGAGGCCACCACGCTGACGGCGCTGCTCAAGGAGGCGGCGGCCGCCTTCCCGACCCGCCGCGCAGTTGCCGTACCGGGCAGGCTGGAGCTCACGCACGCCGCCCTCGACGCTCTCGTCGACGCGGCGGCCGCACGACTCGCCGCCGACGCCGGAGTTCTCCCGGGCCACGTCGTCGCGCTTTCCTTCCCCAACACCGTCGAGCTGGTGATCATGTTCCTGGCGGTAATCCGCGCGCGCGGCGTGGCGGCGCCGCTCAACCCGGCCTACACGCAGGAGGAGTTCGAGTTCTACCTCTCCGACTCGGAGGCGCGCCTCCTCGTCACCAACGCGGAGGGCAACGCGGCGGCGCAGGCGGCCGCTGCCAAGCTCGGCCTCGCCCACGCCGCCGCCAGCCTCCACGACGCGGCCGGCCCTGTCCACCTCGCCGGCCTCCCGGCCCATGCTCCGGAGAACGGGTCCCACGGCGGCGGCGCGGCGGGCTCTCTCTCCCCCAACGACCCGTCGGACGTGGCCCTGTTCCTGCACACCTCCGGCACGACGAGCCGGCCCAAGGGCGTGCCGCTGAAGCAGCGCAACCTGGCGGCGTCGGTGCGGAACATCCGGTTCTCGGCGTCCACGTTCTGGGCCGACATGCGGGCGTCGGGCGCCACCTGGTACACGGCGGTGCCGACGATCCACCAGATCATCCTGGACCGGCACGCGTCGCGGCCGGAGGCGTACCCGGCGCTCCGATTCATCCGCAGCTGCAGCGCGTCGCTGGCGCCGGCGATCCTGGAGCGGCTGGAGGCGGCGTTCAGCGCGCCGGTGCTGGAGGCGTACGCGATGACGGAGGCGTCGCACCTGATGACGTCAAACCCGCTGCCGGAGGACGGGCCACGGAAGCCCGGGTCAGTGGGGCGCGCGGTGGGGCAGGAGCTGGCGGTGCTGGACGAGGAGGGGCGGCTCGTGGCGGCGGGGAGCCCCGGGGAGGTGTGCGTCCGCGGGGACAACGTGACGGCGGGTTACAAGGGGAACCCAGAGGCGAACGAGGCGGCGTTCCGGTTCGGGTGGTTCCACACGGGGGACATCGGCGTGGTGGATGACCAAGGGTACGTCCGGCTGGTGGGGCGCATCAAGGAGCTCATCAACCGCGGCGGGGAGAAGATCTCCCCGATCGAGGTGGACGCCGTGCTGCTGGGCCTCCCCGGTGTGGCGCAGGCGGTGTCGTTCGGGGTGCCTGACGACAAGTACGGGGAGGAGATCAACTGTGCGGTGATCCCGCGGGACGGGTCGGCGCTGCGGGAGGAGGAGGTGCTGGCGCACTGCCGGCGGAACCTGGCGTCGTTCAAGGTGCCCAAGAAGGTGTTCATCACGGACGACCTGCCCAAGACGGCCACCGGAAAGATCCAGCGCCGCATCGTGGCGCAGCACTTCGTGCAGCCGACCAGTGCCTGA
Protein Sequence
  • >Zm00001eb434030_P002
    MAMATTEATTLTALLKEAAAAFPTRRAVAVPGRLELTHAALDALVDAAAARLAADAGVLPGHVVALSFPNTVELVIMFLAVIRARGVAAPLNPAYTQEEFEFYLSDSEARLLVTNAEGNAAAQAAAAKLGLAHAAASLHDAAGPVHLAGLPAHAPENGSHGGGAAGSLSPNDPSDVALFLHTSGTTSRPKGVPLKQRNLAASVRNIRFSASTFWADMRASGATWYTAVPTIHQIILDRHASRPEAYPALRFIRSCSASLAPAILERLEAAFSAPVLEAYAMTEASHLMTSNPLPEDGPRKPGSVGRAVGQELAVLDEEGRLVAAGSPGEVCVRGDNVTAGYKGNPEANEAAFRFGWFHTGDIGVVDDQGYVRLVGRIKELINRGGEKISPIEVDAVLLGLPGVAQAVSFGVPDDKYGEEINCAVIPRDGSALREEEVLAHCRRNLASFKVPKKVFITDDLPKTATGKIQRRIVAQHFVQPTSA
  • >Zm00001eb434030_P001
    MAMATTEATTLTALLKEAAAAFPTRRAVAVPGRLELTHAALDALVDAAAARLAADAGVLPGHVVALSFPNTVELVIMFLAVIRARGVAAPLNPAYTQEEFEFYLSDSEARLLVTNAEGNAAAQAAAAKLGLAHAAASLHDAAGPVHLAGLPAHAPENGSHGGGAAGSLSPNDPSDVALFLHTSGTTSRPKGVPLKQRNLAASVRNIRFSASTFWADMRASGATWYTAVPTIHQIILDRHASRPEAYPALRFIRSCSASLAPAILERLEAAFSAPVLEAYAMTEASHLMTSNPLPEDGPRKPGSVGRAVGQELAVLDEEGRLVAAGSPGEVCVRGDNVTAGYKGNPEANEAAFRFGWFHTGDIGVVDDQGYVRLVGRIKELINRGGEKISPIEVDAVLLGLPGVAQAVSFGVPDDKYGEEINCAVIPRDGSALREEEVLAHCRRNLASFKVPKKVFITDDLPKTATGKIQRRIVAQHFVQPTSA

Source: Genetic information comes from the article