Gene Details:
- Gene ID: Zm00001eb396050
- Gene Symbol: Chr.9_ClpP5|clpp5|v30
- Gene Name: virescent30
- Genome: Zm-B73-REFERENCE-NAM-5.0
- Species: Zea mays
Functional Descriptions:
- Taking the positional cloning approach, the Chr.9_ClpP5|clpp5|v30 gene, one of the proteolytic subunits of the chloroplast Clp protease complex, was identified and validated as the candidate gene for vyl.
- The B73 Chr.9_ClpP5|clpp5|v30 protein consists of 281 amino acids, has a predicted chloroplast transit peptide at the N-terminal and a typical Ser–His–Asp catalytic triad.
- Sequence analysis showed that there were multiple alternative splicing variants of the Chr.9_ClpP5|clpp5|v30 transcripts in the vyl mutant plants, all of which caused major changes in amino acid sequences and resulted in truncated proteins.
- Disruption in vyl-modifier (Chr.1_Chr.9_ClpP5|clpp5|v30) alone does not result in any visible phenotype while loss of vyl (Chr.9_ClpP5|clpp5|v30) results in a chlorotic leaf phenotype and defects in chloroplast thylakoid stacking at seedling stage, suggesting the primary role of Chr.9_ClpP5|clpp5|v30.
Function-related keywords:
- leaf , chloroplast , seedling , protein
Literature:
- A pair of homoeolog ClpP5 genes underlies a virescent yellow-like mutant and its modifier in maize. DOI: 10.1111/tpj.12568 ; PMID: 24888539
Related News:
Gene Resources:
- NCBI ID: LOC100194286
- UniProt accessions: B4FI50
Orthologs:
Sequences:
cDNA Sequence
- >Zm00001eb396050_T004
CTCCGTTACCAGTTACCTCGCCTCGCGGATAAGAGGCGAGGGCTAGCAAACCAAAGCCCCTTCCGCCCTCCGCCCAAAACCCCACGCGAGCACCCCATTCCCATCCATGGCGACCACCACCTCTTCCTCCTCGCTGACCACTCCTCTCCTCCGCCCGAACCCCAATACCAACCCTGCTCCCAGATCTCTCCAACTCCTCAGGAGCAGGAGGTGCGCTCGTGCCGTAACTACCGCCGTAGCCGGAGGTGCGGTGCCTTACGGGGCTAATCCGCGGCGCGGGATTTGGTCGATCAGGAGCGTGGGCCGTCGCCCATGGTGATGGAGCGGTTCCAGAGCGTCGTCAGTCAGCTTTTCCAGCACAGAATTATTCGGTGCGGAGGCCCCGTTGAGGATGATATGGCCAACATCATTGTAGCACAACTGCTCTACCTCGACGCTATTGATCCCAATAAGGATATCATTATGTATGTGAATTCGCCAGGAGGGTCAGTGACAGCTGGAATGGCTATATTTGATACAATGAAGCATATCAGGCCTGATGTATCCACTGTTTGTATTGGACTAGCTGCAAGCATGGGAGCTTTTCTACTTAGTGCTGGGACCAAAGGAAAGCGATACAGCTTACCAAACTCAAGGATAATGATCCATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATACCACACTGGGCAGCCCCTGGATAAGATCAACGTAGATACTGACCGTGACTACTTCATGAGCGCGAAAGAAGCAAAGGAGTATGGCCTTATTGATGGAGTAATCATGAATCCCCTCAAGGCCCTTCAACCACTTCCAGCTTCCAGTTAGTCATGAAGTGTGCTAGAACTCCGTCAAACATTTTTGTTGTATCAATGTGTCTGTTGGAAGCCCTGGCTACGTAATATTCTACGTCCAAATGCATCAAAGATTGTACAAAACAAGATATGCGGTTAGGATATCCATCCTTGGTGATGGATATTGGAACTAAACTAACCTAACCCTTAGAGTTAATTTGTCAACTCTATTTTTCTAAGGAAATTCCTCTCATAAGGCTTGGTTTCCTCGTCTATGTACTCTATATGGATGCCTTTTTTTATAGGACCGATGTCTTTCTATTTTAGGTCAACAGGTGACGGTAATAACTGTGTCTTCATTAGGGCATGTGCCCCCATTAATTAAGGGTTTCTTGAGGGATCAAGTGAGAAAAAAAATCAAGAGATGATTTTCCG - >Zm00001eb396050_T003
CTCCGTTACCAGTTACCTCGCCTCGCGGATAAGAGGCGAGGGCTAGCAAACCAAAGCCCCTTCCGCCCTCCGCCCAAAACCCCACGCGAGCACCCCATTCCCATCCATGGCGACCACCACCTCTTCCTCCTCGCTGACCACTCCTCTCCTCCGCCCGAACCCCAATACCAACCCTGCTCCCAGATCTCTCCAACTCCTCAGGAGCAGGAGGTGCGCTCGTGCCGTAACTACCGCCGTAGCCGGAGGTGCGGTGCCTTACGGGGCTAATCCGCGGCGCGGGATTTGGTCGATCAGGAGCGTGGGCCGTCGCCCATGGTGATGGAGCGGTTCCAGAGCGTCGTCAGTCAGCTTTTCCAGCACAGAATTATTCGGTGCGGAGGCCCCGTTGAGGATGATATGGCCAACATCATTGTAGCACAACTGCTCTACCTCGACGCTATTGATCCCAATAAGGATATCATTATGTATGTGAATTCGCCAGGAGGGTCAGTGACAGCTGGAATGGCTATATTTGATACAATGAAGCATATCAGGCCTGATGTATCCACTGTTTGTATTGGACTAGCTGCAAGCATGGGAGCTTTTCTACTTAGTGCTGGGACCAAAGGAAAGCGATACAGCTTACCAAACTCAAGGATAATGATCCATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATACCACACTGGGCAGCCCCTGGATAAGATCAACGTAGATACTGACCGTGACTACTTCATGAGCGCGAAAGAAGCAAAGGAGTATGGCCTTATTGATGGAGTAATCATGAATCCCCTCAAGGCCCTTCAACCACTTCCAGCTTCCAGTTAGTCATGAAGTGTGCTAGAACTCCGTCAAACATTTTTGTTGTATCAATGTGTCTGTTGGAAGCCCTGGCTACGTAATATTCTACGTCCAAATGCATCAAAGATTGTACAAAACAAGATATGCGGTTAGGATATCCATCCTTGGTGATGGATATTGGAACTAAACTAACCTAACCCTTAGAGTTAATTTGTCAACTCTATTTTTCTAAGGAAATTCCTCTCATAAGGCTTGGTTTCCTCGTCTATGTACTCTATATGGATGCCTTTTTTTATAGGACCGATGTCTTTCTATTTTAGGTCAACAGGTGACGGTAATAACTGTGTCTTCATTAGGGCATGTGCCCCCATTAATTAAGGGTTTCTTGAGGGATCAAGTGAGAAAAAAAATCAAGAGATGATTTTCCG - >Zm00001eb396050_T001
CTCCGTTACCAGTTACCTCGCCTCGCGGATAAGAGGCGAGGGCTAGCAAACCAAAGCCCCTTCCGCCCTCCGCCCAAAACCCCACGCGAGCACCCCATTCCCATCCATGGCGACCACCACCTCTTCCTCCTCGCTGACCACTCCTCTCCTCCGCCCGAACCCCAATACCAACCCTGCTCCCAGATCTCTCCAACTCCTCAGGAGCAGGAGGTGCGCTCGTGCCGTAACTACCGCCGTAGCCGGAGGTGCGGTGCCTTACGGGGCTAATCCGCGGCGCGGGATTTGGTCGATCAGGAGCGTGGGCCGTCGCCCATGGTGATGGAGCGGTTCCAGAGCGTCGTCAGTCAGCTTTTCCAGCACAGAATTATTCGGTGCGGAGGCCCCGTTGAGGATGATATGGCCAACATCATTGTAGCACAACTGCTCTACCTCGACGCTATTGATCCCAATAAGGATATCATTATGTATGTGAATTCGCCAGGAGGGTCAGTGACAGCTGGAATGGCTATATTTGATACAATGAAGCATATCAGGCCTGATGTATCCACTGTTTGTATTGGACTAGCTGCAAGCATGGGAGCTTTTCTACTTAGTGCTGGGACCAAAGGAAAGCGATACAGCTTACCAAACTCAAGGATAATGATCCATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATACCACACTGGGCAGCCCCTGGATAAGATCAACGTAGATACTGACCGTGACTACTTCATGAGCGCGAAAGAAGCAAAGGAGTATGGCCTTATTGATGGAGTAATCATGAATCCCCTCAAGGCCCTTCAACCACTTCCAGCTTCCAGTTAGTCATGAAGTGTGCTAGAACTCCGTCAAACATTTTTGTTGTATCAATGTGTCTGTTGGAAGCCCTGGCTACGTAATATTCTACGTCCAAATGCATCAAAGATTGTACAAAACAAGATATGCGGTTAGGATATCCATCCTTGGTGATGGATATTGGAACTAAACTAACCTAACCCTTAGAGTTAATTTGTCAACTCTATTTTTCTAAGGAAATTCCTCTCATAAGGCTTGGTTTCCTCGTCTATGTACTCTATATGGATGCCTTTTTTTATAGGACCGATGTCTTTCTATTTTAGGTCAACAGGTGACGGTAATAACTGTGTCTTCATTAGGGCATGTGCCCCCATTAATTAAGGGTTTCTTGAGGGATCAAGTGAGAAAAAAAATCAAGAGATGATTTTCCG - >Zm00001eb396050_T002
CTCCGTTACCAGTTACCTCGCCTCGCGGATAAGAGGCGAGGGCTAGCAAACCAAAGCCCCTTCCGCCCTCCGCCCAAAACCCCACGCGAGCACCCCATTCCCATCCATGGCGACCACCACCTCTTCCTCCTCGCTGACCACTCCTCTCCTCCGCCCGAACCCCAATACCAACCCTGCTCCCAGATCTCTCCAACTCCTCAGGAGCAGGAGGTGCGCTCGTGCCGTAACTACCGCCGTAGCCGGAGGTGCGGTGCCTTACGGGGCTAATCCGCGGCGCGGGATTTGGTCGATCAGGAGCGTGGGCCGTCGCCCATGGTGATGGAGCGGTTCCAGAGCGTCGTCAGTCAGCTTTTCCAGCACAGAATTATTCGGTGCGGAGGCCCCGTTGAGGATGATATGGCCAACATCATTGTAGCACAACTGCTCTACCTCGACGCTATTGATCCCAATAAGGATATCATTATGTATGTGAATTCGCCAGGAGGGTCAGTGACAGCTGGAATGGCTATATTTGATACAATGAAGCATATCAGGCCTGATGTATCCACTGTTTGTATTGGACTAGCTGCAAGCATGGGAGCTTTTCTACTTAGTGCTGGGACCAAAGGAAAGCGATACAGCTTACCAAACTCAAGGATAATGATCCATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATACCACACTGGGCAGCCCCTGGATAAGATCAACGTAGATACTGACCGTGACTACTTCATGAGCGCGAAAGAAGCAAAGGAGTATGGCCTTATTGATGGAGTAATCATGAATCCCCTCAAGGCCCTTCAACCACTTCCAGCTTCCAGTTAGTCATGAAGTGTGCTAGAACTCCGTCAAACATTTTTGTTGTATCAATGTGTCTGTTGGAAGCCCTGGCTACGTAATATTCTACGTCCAAATGCATCAAAGATTGTACAAAACAAGATATGCGGTTAGGATATCCATCCTTGGTGATGGATATTGGAACTAAACTAACCTAACCCTTAGAGTTAATTTGTCAACTCTATTTTTCTAAGGAAATTCCTCTCATAAGGCTTGGTTTCCTCGTCTATGTACTCTATATGGATGCCTTTTTTTATAGGACCGATGTCTTTCTATTTTAGGTCAACAGGTGACGGTAATAACTGTGTCTTCATTAGGGCATGTGCCCCCATTAATTAAGGGTTTCTTGAGGGATCAAGTGAGAAAAAAAATCAAGAGATGATTTTCCG - >Zm00001eb396050_T005
CTCCGTTACCAGTTACCTCGCCTCGCGGATAAGAGGCGAGGGCTAGCAAACCAAAGCCCCTTCCGCCCTCCGCCCAAAACCCCACGCGAGCACCCCATTCCCATCCATGGCGACCACCACCTCTTCCTCCTCGCTGACCACTCCTCTCCTCCGCCCGAACCCCAATACCAACCCTGCTCCCAGATCTCTCCAACTCCTCAGGAGCAGGAGGTGCGCTCGTGCCGTAACTACCGCCGTAGCCGGAGGTGCGGTGCCTTACGGGGCTAATCCGCGGCGCGGGATTTGGTCGATCAGGAGCGTGGGCCGTCGCCCATGGTGATGGAGCGGTTCCAGAGCGTCGTCAGTCAGCTTTTCCAGCACAGAATTATTCGGTGCGGAGGCCCCGTTGAGGATGATATGGCCAACATCATTGTAGCACAACTGCTCTACCTCGACGCTATTGATCCCAATAAGGATATCATTATGTATGTGAATTCGCCAGGAGGGTCAGTGACAGCTGGAATGGCTATATTTGATACAATGAAGCATATCAGGCCTGATGTATCCACTGTTTGTATTGGACTAGCTGCAAGCATGGGAGCTTTTCTACTTAGTGCTGGGACCAAAGGAAAGCGATACAGCTTACCAAACTCAAGGATAATGATCCATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATACCACACTGGGCAGCCCCTGGATAAGATCAACGTAGATACTGACCGTGACTACTTCATGAGCGCGAAAGAAGCAAAGGAGTATGGCCTTATTGATGGAGTAATCATGAATCCCCTCAAGGCCCTTCAACCACTTCCAGCTTCCAGTTAGTCATGAAGTGTGCTAGAACTCCGTCAAACATTTTTGTTGTATCAATGTGTCTGTTGGAAGCCCTGGCTACGTAATATTCTACGTCCAAATGCATCAAAGATTGTACAAAACAAGATATGCGGTTAGGATATCCATCCTTGGTGATGGATATTGGAACTAAACTAACCTAACCCTTAGAGTTAATTTGTCAACTCTATTTTTCTAAGGAAATTCCTCTCATAAGGCTTGGTTTCCTCGTCTATGTACTCTATATGGATGCCTTTTTTTATAGGACCGATGTCTTTCTATTTTAGGTCAACAGGTGACGGTAATAACTGTGTCTTCATTAGGGCATGTGCCCCCATTAATTAAGGGTTTCTTGAGGGATCAAGTGAGAAAAAAAATCAAGAGATGATTTTCCG
CDS Sequence
- >Zm00001eb396050_T004
ATGGTGATGGAGCGGTTCCAGAGCGTCGTCAGTCAGCTTTTCCAGCACAGAATTATTCGGTGCGGAGGCCCCGTTGAGGATGATATGGCCAACATCATTGTAGCACAACTGCTCTACCTCGACGCTATTGATCCCAATAAGGATATCATTATGTATGTGAATTCGCCAGGAGGGTCAGTGACAGCTGGAATGGCTATATTTGATACAATGAAGCATATCAGGCCTGATGTATCCACTGTTTGTATTGGACTAGCTGCAAGCATGGGAGCTTTTCTACTTAGTGCTGGGACCAAAGGAAAGCGATACAGCTTACCAAACTCAAGGATAATGATCCATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATACCACACTGGGCAGCCCCTGGATAAGATCAACGTAGATACTGACCGTGACTACTTCATGAGCGCGAAAGAAGCAAAGGAGTATGGCCTTATTGATGGAGTAATCATGAATCCCCTCAAGGCCCTTCAACCACTTCCAGCTTCCAGTTAG - >Zm00001eb396050_T003
ATGGTGATGGAGCGGTTCCAGAGCGTCGTCAGTCAGCTTTTCCAGCACAGAATTATTCGGTGCGGAGGCCCCGTTGAGGATGATATGGCCAACATCATTGTAGCACAACTGCTCTACCTCGACGCTATTGATCCCAATAAGGATATCATTATGTATGTGAATTCGCCAGGAGGGTCAGTGACAGCTGGAATGGCTATATTTGATACAATGAAGCATATCAGGCCTGATGTATCCACTGTTTGTATTGGACTAGCTGCAAGCATGGGAGCTTTTCTACTTAGTGCTGGGACCAAAGGAAAGCGATACAGCTTACCAAACTCAAGGATAATGATCCATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATACCACACTGGGCAGCCCCTGGATAAGATCAACGTAGATACTGACCGTGACTACTTCATGAGCGCGAAAGAAGCAAAGGAGTATGGCCTTATTGATGGAGTAATCATGAATCCCCTCAAGGCCCTTCAACCACTTCCAGCTTCCAGTTAG - >Zm00001eb396050_T001
ATGGTGATGGAGCGGTTCCAGAGCGTCGTCAGTCAGCTTTTCCAGCACAGAATTATTCGGTGCGGAGGCCCCGTTGAGGATGATATGGCCAACATCATTGTAGCACAACTGCTCTACCTCGACGCTATTGATCCCAATAAGGATATCATTATGTATGTGAATTCGCCAGGAGGGTCAGTGACAGCTGGAATGGCTATATTTGATACAATGAAGCATATCAGGCCTGATGTATCCACTGTTTGTATTGGACTAGCTGCAAGCATGGGAGCTTTTCTACTTAGTGCTGGGACCAAAGGAAAGCGATACAGCTTACCAAACTCAAGGATAATGATCCATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATACCACACTGGGCAGCCCCTGGATAAGATCAACGTAGATACTGACCGTGACTACTTCATGAGCGCGAAAGAAGCAAAGGAGTATGGCCTTATTGATGGAGTAATCATGAATCCCCTCAAGGCCCTTCAACCACTTCCAGCTTCCAGTTAG - >Zm00001eb396050_T002
ATGGTGATGGAGCGGTTCCAGAGCGTCGTCAGTCAGCTTTTCCAGCACAGAATTATTCGGTGCGGAGGCCCCGTTGAGGATGATATGGCCAACATCATTGTAGCACAACTGCTCTACCTCGACGCTATTGATCCCAATAAGGATATCATTATGTATGTGAATTCGCCAGGAGGGTCAGTGACAGCTGGAATGGCTATATTTGATACAATGAAGCATATCAGGCCTGATGTATCCACTGTTTGTATTGGACTAGCTGCAAGCATGGGAGCTTTTCTACTTAGTGCTGGGACCAAAGGAAAGCGATACAGCTTACCAAACTCAAGGATAATGATCCATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATACCACACTGGGCAGCCCCTGGATAAGATCAACGTAGATACTGACCGTGACTACTTCATGAGCGCGAAAGAAGCAAAGGAGTATGGCCTTATTGATGGAGTAATCATGAATCCCCTCAAGGCCCTTCAACCACTTCCAGCTTCCAGTTAG - >Zm00001eb396050_T005
ATGGTGATGGAGCGGTTCCAGAGCGTCGTCAGTCAGCTTTTCCAGCACAGAATTATTCGGTGCGGAGGCCCCGTTGAGGATGATATGGCCAACATCATTGTAGCACAACTGCTCTACCTCGACGCTATTGATCCCAATAAGGATATCATTATGTATGTGAATTCGCCAGGAGGGTCAGTGACAGCTGGAATGGCTATATTTGATACAATGAAGCATATCAGGCCTGATGTATCCACTGTTTGTATTGGACTAGCTGCAAGCATGGGAGCTTTTCTACTTAGTGCTGGGACCAAAGGAAAGCGATACAGCTTACCAAACTCAAGGATAATGATCCATCAACCTCTTGGAGGGGCCCAAGGACAGGAGACTGATCTTGAGATTCAGGCCAATGAGATGCTGCACCACAAGGCGAACTTAAATGGATACCTTGCATACCACACTGGGCAGCCCCTGGATAAGATCAACGTAGATACTGACCGTGACTACTTCATGAGCGCGAAAGAAGCAAAGGAGTATGGCCTTATTGATGGAGTAATCATGAATCCCCTCAAGGCCCTTCAACCACTTCCAGCTTCCAGTTAG
Protein Sequence
- >Zm00001eb396050_P004
MVMERFQSVVSQLFQHRIIRCGGPVEDDMANIIVAQLLYLDAIDPNKDIIMYVNSPGGSVTAGMAIFDTMKHIRPDVSTVCIGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGQETDLEIQANEMLHHKANLNGYLAYHTGQPLDKINVDTDRDYFMSAKEAKEYGLIDGVIMNPLKALQPLPASS - >Zm00001eb396050_P003
MVMERFQSVVSQLFQHRIIRCGGPVEDDMANIIVAQLLYLDAIDPNKDIIMYVNSPGGSVTAGMAIFDTMKHIRPDVSTVCIGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGQETDLEIQANEMLHHKANLNGYLAYHTGQPLDKINVDTDRDYFMSAKEAKEYGLIDGVIMNPLKALQPLPASS - >Zm00001eb396050_P001
MVMERFQSVVSQLFQHRIIRCGGPVEDDMANIIVAQLLYLDAIDPNKDIIMYVNSPGGSVTAGMAIFDTMKHIRPDVSTVCIGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGQETDLEIQANEMLHHKANLNGYLAYHTGQPLDKINVDTDRDYFMSAKEAKEYGLIDGVIMNPLKALQPLPASS - >Zm00001eb396050_P002
MVMERFQSVVSQLFQHRIIRCGGPVEDDMANIIVAQLLYLDAIDPNKDIIMYVNSPGGSVTAGMAIFDTMKHIRPDVSTVCIGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGQETDLEIQANEMLHHKANLNGYLAYHTGQPLDKINVDTDRDYFMSAKEAKEYGLIDGVIMNPLKALQPLPASS - >Zm00001eb396050_P005
MVMERFQSVVSQLFQHRIIRCGGPVEDDMANIIVAQLLYLDAIDPNKDIIMYVNSPGGSVTAGMAIFDTMKHIRPDVSTVCIGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGQETDLEIQANEMLHHKANLNGYLAYHTGQPLDKINVDTDRDYFMSAKEAKEYGLIDGVIMNPLKALQPLPASS
Source: Genetic information comes from the article