Gene Details:

Functional Descriptions:

  • Tie-dyed2 Encodes a Callose Synthase That Functions in Vein Development and Affects Symplastic Trafficking within the Phloem of Maize Leaves.
  • The tie-dyed2 (tdy2) mutant of maize (Zea mays) displays variegated green and yellow leaves. Intriguingly, the yellow leaf tissues hyperaccumulate starch and sucrose, the soluble sugar transported long distance through the phloem of veins.
  • tdy2 was most highly expressed in the vascular tissue. Comparative expression analysis with the vascular marker maize PINFORMED1a-yellow fluorescent protein confirmed that tdy2 was expressed in developing vein tissues.
  • The disruption of sucrose movement in the phloem in tdy2 mutants provides evidence that the tdy2 callose synthase functions in vascular maturation and that the vascular defects result in impaired symplastic trafficking into the phloem translocation stream.
  • The results showed that tdy2 yellow leaf regions were defective in phloem export but competent in long-distance transport.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Zm00001eb259620_T001
    AATCCTTCTGGGTTCGAGTGGCAGAAAATTGTGGATGACTGGACTGACTGGAATAAGTGGATCAGTAACCGTGGAGGTATTGGGGTGCCGCCAGAGAAAAGTTGGGAGTCATGGTGGGAAAAAGAGCAGGAGCCTCTTAGGCATTCTGGAAAGCGTGGCACTGTTCTTGAAATAGTGCTTTCGTTGCGCTTCTTTATTTACCAGTATGGTCTTGTTTATCATTTGAACATCACCACTCACACGAAGAGCGTTCTGGTTTATTGCATCTCGTGGGTCATAATCTTTGTCATTTTGCTCGTGATGAAGACTGTATCAGTTGGCAGGAGGAAATTCAGTGCAGAGTTCCAGCTTGTGTTCCGGCTGATCAAGGGGCTGATATTCATAACGTTTATCTCTATTATCATAATCTTGATAGCAATCCCACACATGACAGTTCAGGACATCTTTGTTTGCATCCTTGCGTTCATGCCAACTGGATGGGGTTTGCTTCTGATAGCACAAACTATGAGGTCAGCTATTTCACATATGGGGCTATGGGGATCGGTCAAGGCGCTTGCCCGAGGCTATGAGATCATCATGGGCTTGCTGTTGTTCACCCCGATCGCATTCCTCGCTTGGTTCCCGTTTGTTTCGGAGTTCCAGACAAGGATGCTGTTCAACCAGGCCTTCAGTAGAGGTCTGCAGATTTCTCGTATCCTAGGCGGACACAAGAAAGACCGGGCTGCACGGAACAAGGAGTGAGTAGCCGAGCAGCGTGAGCCTGGTAGTGCTGACCTACATACAATTCAACCATGCCCCACGTGG
CDS Sequence
  • >Zm00001eb259620_T001
    ATGAAGACTGTATCAGTTGGCAGGAGGAAATTCAGTGCAGAGTTCCAGCTTGTGTTCCGGCTGATCAAGGGGCTGATATTCATAACGTTTATCTCTATTATCATAATCTTGATAGCAATCCCACACATGACAGTTCAGGACATCTTTGTTTGCATCCTTGCGTTCATGCCAACTGGATGGGGTTTGCTTCTGATAGCACAAACTATGAGGTCAGCTATTTCACATATGGGGCTATGGGGATCGGTCAAGGCGCTTGCCCGAGGCTATGAGATCATCATGGGCTTGCTGTTGTTCACCCCGATCGCATTCCTCGCTTGGTTCCCGTTTGTTTCGGAGTTCCAGACAAGGATGCTGTTCAACCAGGCCTTCAGTAGAGGTCTGCAGATTTCTCGTATCCTAGGCGGACACAAGAAAGACCGGGCTGCACGGAACAAGGAGTGA
Protein Sequence
  • >Zm00001eb259620_P001
    MKTVSVGRRKFSAEFQLVFRLIKGLIFITFISIIIILIAIPHMTVQDIFVCILAFMPTGWGLLLIAQTMRSAISHMGLWGSVKALARGYEIIMGLLLFTPIAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGHKKDRAARNKE

Source: Genetic information comes from the article