Gene Details:
- Gene ID: Zm00001eb247820
- Gene Symbol: ZmDEF1
- Gene Name: defensin 1
- Genome: Zm-B73-REFERENCE-NAM-5.0
- Species: Zea mays
Functional Descriptions:
- Overexpression of the ZmDEF1 gene increases the resistance to weevil larvae in transgenic maize seeds.
- The Zea mays defensin 1 (ZmDEF1) gene encodes the defensin 1 protein, which can inhibit alpha-amylase in the insect gut.
- ZmDEF1 was cloned into a pBetaPhaso-dest vector, which harbours phaseolin, a seed-specific promoter, and the Agrobacterium tumefaciens strain C58 harbouring the pBetaPhaso-ZmDEF1 vector was used to transfer the ZmDEF1 gene into two maize cultivars using immature embryos.
- The recombinant ZmDEF1 protein inhibited the alpha-amylase activity of the maize weevil larvae, and its ability to inhibit alpha-amylase is 54.52–63.09% greater than the ZmDEF1 protein extracted from non-transgenic plants.
Function-related keywords:
- resistance , seed , insect , insect-resistance , protein
Literature:
- Overexpression of the ZmDEF1 gene increases the resistance to weevil larvae in transgenic maize seeds. DOI: 10.1007/s11033-019-04670-5 ; PMID: 30798487
Related News:
Gene Resources:
- NCBI ID: LOC100282320
- UniProt accessions: A0A3L6EJV4
Orthologs:
Sequences:
cDNA Sequence
- >Zm00001eb247820_T002
GTATCCGCGAGGATCCCCCCACACCTTGCGACCTGCGTTGTCACCCACCCATCAAGGTTGGGGCCGCCAGCAGGTTCAGCCGTTCCTGTTCTTGATAAAACGAGAGAAGGATGGCGGCAGTGTCTCAGGGAGCTGTCCTATTCTTGTTTCTCCTCCTCGTCGCAGCAGCCATGCCCATGCATGCCTTGATAATGGAGAACGTGAAACAGCAGGAGAAGGAGAAGGAGAAGGAGAAGGAGGAGAAAAGCACGGAGAAGGAAGAGAGTCGATGCTTATCGCAGAGTCTCCAGTTCGAGGGCTTCTGCTTCAACAGCGACAGATGCGCCGAGGTGTGCATGAAGGAGAGCTTTCCCGGTGGCGAGTGCAAGCGGGACGTGGCCATGCGCAAGTGCTTCTGCAAGAAGCCTTGCTAGTTCATCGGTCTTGCTAATTGTTGATGGGTGCTTCATTAATTTGATTTGATGGGTCTCATGGCTACGAGAAACAAAACTACTTTTGATAAAATGCAGGGCATGAAAAGCGTTTCTGCAGTCCTTAAATTGCTGGGTTTTTTTTTGACGAAACTGCATGGAGGGGTTTGCAGCCCCTT - >Zm00001eb247820_T003
GTATCCGCGAGGATCCCCCCACACCTTGCGACCTGCGTTGTCACCCACCCATCAAGGTTGGGGCCGCCAGCAGGTTCAGCCGTTCCTGTTCTTGATAAAACGAGAGAAGGATGGCGGCAGTGTCTCAGGGAGCTGTCCTATTCTTGTTTCTCCTCCTCGTCGCAGCAGCCATGCCCATGCATGCCTTGATAATGGAGAACGTGAAACAGCAGGAGAAGGAGAAGGAGAAGGAGAAGGAGGAGAAAAGCACGGAGAAGGAAGAGAGTCGATGCTTATCGCAGAGTCTCCAGTTCGAGGGCTTCTGCTTCAACAGCGACAGATGCGCCGAGGTGTGCATGAAGGAGAGCTTTCCCGGTGGCGAGTGCAAGCGGGACGTGGCCATGCGCAAGTGCTTCTGCAAGAAGCCTTGCTAGTTCATCGGTCTTGCTAATTGTTGATGGGTGCTTCATTAATTTGATTTGATGGGTCTCATGGCTACGAGAAACAAAACTACTTTTGATAAAATGCAGGGCATGAAAAGCGTTTCTGCAGTCCTTAAATTGCTGGGTTTTTTTTTGACGAAACTGCATGGAGGGGTTTGCAGCCCCTT - >Zm00001eb247820_T001
GTATCCGCGAGGATCCCCCCACACCTTGCGACCTGCGTTGTCACCCACCCATCAAGGTTGGGGCCGCCAGCAGGTTCAGCCGTTCCTGTTCTTGATAAAACGAGAGAAGGATGGCGGCAGTGTCTCAGGGAGCTGTCCTATTCTTGTTTCTCCTCCTCGTCGCAGCAGCCATGCCCATGCATGCCTTGATAATGGAGAACGTGAAACAGCAGGAGAAGGAGAAGGAGAAGGAGAAGGAGGAGAAAAGCACGGAGAAGGAAGAGAGTCGATGCTTATCGCAGAGTCTCCAGTTCGAGGGCTTCTGCTTCAACAGCGACAGATGCGCCGAGGTGTGCATGAAGGAGAGCTTTCCCGGTGGCGAGTGCAAGCGGGACGTGGCCATGCGCAAGTGCTTCTGCAAGAAGCCTTGCTAGTTCATCGGTCTTGCTAATTGTTGATGGGTGCTTCATTAATTTGATTTGATGGGTCTCATGGCTACGAGAAACAAAACTACTTTTGATAAAATGCAGGGCATGAAAAGCGTTTCTGCAGTCCTTAAATTGCTGGGTTTTTTTTTGACGAAACTGCATGGAGGGGTTTGCAGCCCCTT
CDS Sequence
- >Zm00001eb247820_T002
ATGGCGGCAGTGTCTCAGGGAGCTGTCCTATTCTTGTTTCTCCTCCTCGTCGCAGCAGCCATGCCCATGCATGCCTTGATAATGGAGAACGTGAAACAGCAGGAGAAGGAGAAGGAGAAGGAGAAGGAGGAGAAAAGCACGGAGAAGGAAGAGAGTCGATGCTTATCGCAGAGTCTCCAGTTCGAGGGCTTCTGCTTCAACAGCGACAGATGCGCCGAGGTGTGCATGAAGGAGAGCTTTCCCGGTGGCGAGTGCAAGCGGGACGTGGCCATGCGCAAGTGCTTCTGCAAGAAGCCTTGCTAG - >Zm00001eb247820_T003
ATGGCGGCAGTGTCTCAGGGAGCTGTCCTATTCTTGTTTCTCCTCCTCGTCGCAGCAGCCATGCCCATGCATGCCTTGATAATGGAGAACGTGAAACAGCAGGAGAAGGAGAAGGAGAAGGAGAAGGAGGAGAAAAGCACGGAGAAGGAAGAGAGTCGATGCTTATCGCAGAGTCTCCAGTTCGAGGGCTTCTGCTTCAACAGCGACAGATGCGCCGAGGTGTGCATGAAGGAGAGCTTTCCCGGTGGCGAGTGCAAGCGGGACGTGGCCATGCGCAAGTGCTTCTGCAAGAAGCCTTGCTAG - >Zm00001eb247820_T001
ATGGCGGCAGTGTCTCAGGGAGCTGTCCTATTCTTGTTTCTCCTCCTCGTCGCAGCAGCCATGCCCATGCATGCCTTGATAATGGAGAACGTGAAACAGCAGGAGAAGGAGAAGGAGAAGGAGAAGGAGGAGAAAAGCACGGAGAAGGAAGAGAGTCGATGCTTATCGCAGAGTCTCCAGTTCGAGGGCTTCTGCTTCAACAGCGACAGATGCGCCGAGGTGTGCATGAAGGAGAGCTTTCCCGGTGGCGAGTGCAAGCGGGACGTGGCCATGCGCAAGTGCTTCTGCAAGAAGCCTTGCTAG
Protein Sequence
- >Zm00001eb247820_P002
MAAVSQGAVLFLFLLLVAAAMPMHALIMENVKQQEKEKEKEKEEKSTEKEESRCLSQSLQFEGFCFNSDRCAEVCMKESFPGGECKRDVAMRKCFCKKPC - >Zm00001eb247820_P003
MAAVSQGAVLFLFLLLVAAAMPMHALIMENVKQQEKEKEKEKEEKSTEKEESRCLSQSLQFEGFCFNSDRCAEVCMKESFPGGECKRDVAMRKCFCKKPC - >Zm00001eb247820_P001
MAAVSQGAVLFLFLLLVAAAMPMHALIMENVKQQEKEKEKEKEEKSTEKEESRCLSQSLQFEGFCFNSDRCAEVCMKESFPGGECKRDVAMRKCFCKKPC
Source: Genetic information comes from the article