Gene Details:

Functional Descriptions:

  • An allele of ZmPORB2 encoding a protochlorophyllide oxidoreductase promotes tocopherol accumulation in both leaves and kernels of maiz.
  • Overexpression of ZmPORB2 increased tocopherol content in both leaves and kernels. Candidate gene association analysis identified a 5/8-bp insertion/deletion (InDel058) in the 5′ untranslated region (UTR) as the causal polymorphism in affecting ZmPORB2 expression and being highly associated with tocopherol content.
  • RNA-sequencing and metabolic analysis in near isogenic lines (NILs) support that ZmPORB2 participates in chlorophyll metabolism enabling the production of phytol, an important precursor of tocopherol.
  • Finally, we posit that a PCR marker derived from the causative polymorphism of ZmPORB2 could be utilized in high vitamin E maize breeding.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Zm00001eb220940_T001
    CCCTATCGCGTTATCGCCACCGGCTCACCGCTCACGGTCACGGCCGAATAAACTCCCGCGGTGCCGTGCCTCGCTTTCGCATCCCCCCGCGGAGAGGAGAGGAAGAAGAAGAACCCAGCCAAATCCTCAGTCTTCAGGCTGCTCACAGCTCGTGCCGTCCACTCTCCCCCGAGGCAGTCTCTTGCGTTCGCTGCTCGACATGGCGCTCCAGGCGGCGACGTCCTTTCTCCCCTCGGCCCTCTCCGCGCGCAAGGAGGGGTCGGTGAAGGACTCGGCGTCGTTCTTGGGTGTTCGTCTCGCGGCGGATGGCCTCAAGCTGGACACCACCGCTCTGGGCCTACGCACCGTGAGGGTGAGCAGGTCGGCGGACATCCGCGCGCAGACGGCAGCGGTGTCCTCCCCGTCAGTGACCCCCGCGTCGCCGTCTGGCAAGAAGACCCTCCGCAAGGGCACGGCGGTCATCACCGGCGCGTCGTCCGGCCTCGGCCTCGCCACGGCGAAGGCCCTCGCGGAGACAGGCAAGTGGCACGTCATCATGGCCTGCCGCGACTTCCTCAAGGCGTCGCGCGCGGCCAAGGCGGCCGGCATGGACAAGGACAGCTTCACCGTCGTGCACCTGGACCTCGCCTCCCTGGACAGCGTCCGCCAGTTCGTCAGGAACGTGCGCCAGCTGGAGATGCCCATCGACGTGGTGGTCTGCAACGCCGCCGTGTACCAGCCCACCGCCAAGGAGCCGTCCTACACCGCCGACGGCTTCGAGATGAGCGTCGGCGTCAACCACCTCGGCCATTTCCTCCTCGCCCGCGAGCTCCTCAGCGACCTCCAGTCCTCCGACTACCCCTCTAAGCGCCTCATCATCGTCGGCTCCATCACCGCAGGGAACACGAACACGCTGGCGGGGAACGTGCCCCCGAAGGCGAACCTGGGCGACCTGCGCGGCCTCGCCGGCGGCCTCAACGGCGTTGGCAGCTCGGTGATGATCGACGGCGGGGAGTTCGACGGCGCCAAGGCATACAAGGACAGCAAGGTGTGCAACATGCTGACGATGCAGGAGTTCCACCGCCGGTACCACGAGGAGACGGGCGTGACCTTCGCGTCGCTCTACCCGGGCTGCATCGCCACCACGGGCCTGTTCCGCGAGCACATCCCGCTGTTCCGCCTGCTCTTCCCGCCGTTCCAGAAGTACATCACCAAGGGGTACGTCTCCGAGGAGGAGGCCGGGAAGCGGCTGGCGCAGGTGGTGAGCGACCCCAGCCTGACCAAGTCCGGCGTGTACTGGAGCTGGAACAAGAACTCCGCGTCCTTCGAGAACCAGCTCTCTGAGGAGGCCAGCGACGCCGACAAGGCCAAGAAGCTCTGGGAGATCAGCGAGAAGCTCGTCGGCTTGGCGTGATGCCCACCGTGGCCGGCGCCGGCAGCCGGCGACAGTTTTTCCTACCTAGGACATGCTCATTAGTTGGTCTCACTCTCAGTCGAGTAGTCGACGTGTGGTGCGCCCGTGTCAGAGTGCCAAGCCAAGACAGAAAGTGAGTACAAACGTGTAAAATATGTGCTACTGCGACGTGATGGTTTGGGCAGTGGACTGTATGTATCTCTACTCTCTAGGCTTCTGCTTTGCTTTGCATATTTAATTTTCTGCTAATAAGAGGACAGCAAATGCCTTTCGAGCTTCTTTACTTTGTCGGCATTTAGCAGATTCAGATTTTTCTTGAGGACATGGCACGTGAATAATTTCTTCTGTCAGTAGTTCAGTACAAGTTCTCCAATGTTTTTTGTATCCAGCTACTCAAATGTTCCCAGCACAGAATGACACACTTCAATCCATTTCCATTCTCACTGTACACGAAAGTAAACTAGGATAGGGACCTGCATGTTCTAAAAAATATTTCAGATTCAGATTATTTCCAG
  • >Zm00001eb220940_T002
    CCCTATCGCGTTATCGCCACCGGCTCACCGCTCACGGTCACGGCCGAATAAACTCCCGCGGTGCCGTGCCTCGCTTTCGCATCCCCCCGCGGAGAGGAGAGGAAGAAGAAGAACCCAGCCAAATCCTCAGTCTTCAGGCTGCTCACAGCTCGTGCCGTCCACTCTCCCCCGAGGCAGTCTCTTGCGTTCGCTGCTCGACATGGCGCTCCAGGCGGCGACGTCCTTTCTCCCCTCGGCCCTCTCCGCGCGCAAGGAGGGGTCGGTGAAGGACTCGGCGTCGTTCTTGGGTGTTCGTCTCGCGGCGGATGGCCTCAAGCTGGACACCACCGCTCTGGGCCTACGCACCGTGAGGGTGAGCAGGTCGGCGGACATCCGCGCGCAGACGGCAGCGGTGTCCTCCCCGTCAGTGACCCCCGCGTCGCCGTCTGGCAAGAAGACCCTCCGCAAGGGCACGGCGGTCATCACCGGCGCGTCGTCCGGCCTCGGCCTCGCCACGGCGAAGGCCCTCGCGGAGACAGGCAAGTGGCACGTCATCATGGCCTGCCGCGACTTCCTCAAGGCGTCGCGCGCGGCCAAGGCGGCCGGCATGGACAAGGACAGCTTCACCGTCGTGCACCTGGACCTCGCCTCCCTGGACAGCGTCCGCCAGTTCGTCAGGAACGTGCGCCAGCTGGAGATGCCCATCGACGTGGTGGTCTGCAACGCCGCCGTGTACCAGCCCACCGCCAAGGAGCCGTCCTACACCGCCGACGGCTTCGAGATGAGCGTCGGCGTCAACCACCTCGGCCATTTCCTCCTCGCCCGCGAGCTCCTCAGCGACCTCCAGTCCTCCGACTACCCCTCTAAGCGCCTCATCATCGTCGGCTCCATCACCGCAGGGAACACGAACACGCTGGCGGGGAACGTGCCCCCGAAGGCGAACCTGGGCGACCTGCGCGGCCTCGCCGGCGGCCTCAACGGCGTTGGCAGCTCGGTGATGATCGACGGCGGGGAGTTCGACGGCGCCAAGGCATACAAGGACAGCAAGGTGTGCAACATGCTGACGATGCAGGAGTTCCACCGCCGGTACCACGAGGAGACGGGCGTGACCTTCGCGTCGCTCTACCCGGGCTGCATCGCCACCACGGGCCTGTTCCGCGAGCACATCCCGCTGTTCCGCCTGCTCTTCCCGCCGTTCCAGAAGTACATCACCAAGGGGTACGTCTCCGAGGAGGAGGCCGGGAAGCGGCTGGCGCAGGTGGTGAGCGACCCCAGCCTGACCAAGTCCGGCGTGTACTGGAGCTGGAACAAGAACTCCGCGTCCTTCGAGAACCAGCTCTCTGAGGAGGCCAGCGACGCCGACAAGGCCAAGAAGCTCTGGGAGATCAGCGAGAAGCTCGTCGGCTTGGCGTGATGCCCACCGTGGCCGGCGCCGGCAGCCGGCGACAGTTTTTCCTACCTAGGACATGCTCATTAGTTGGTCTCACTCTCAGTCGAGTAGTCGACGTGTGGTGCGCCCGTGTCAGAGTGCCAAGCCAAGACAGAAAGTGAGTACAAACGTGTAAAATATGTGCTACTGCGACGTGATGGTTTGGGCAGTGGACTGTATGTATCTCTACTCTCTAGGCTTCTGCTTTGCTTTGCATATTTAATTTTCTGCTAATAAGAGGACAGCAAATGCCTTTCGAGCTTCTTTACTTTGTCGGCATTTAGCAGATTCAGATTTTTCTTGAGGACATGGCACGTGAATAATTTCTTCTGTCAGTAGTTCAGTACAAGTTCTCCAATGTTTTTTGTATCCAGCTACTCAAATGTTCCCAGCACAGAATGACACACTTCAATCCATTTCCATTCTCACTGTACACGAAAGTAAACTAGGATAGGGACCTGCATGTTCTAAAAAATATTTCAGATTCAGATTATTTCCAG
  • >Zm00001eb220940_T003
    CCCTATCGCGTTATCGCCACCGGCTCACCGCTCACGGTCACGGCCGAATAAACTCCCGCGGTGCCGTGCCTCGCTTTCGCATCCCCCCGCGGAGAGGAGAGGAAGAAGAAGAACCCAGCCAAATCCTCAGTCTTCAGGCTGCTCACAGCTCGTGCCGTCCACTCTCCCCCGAGGCAGTCTCTTGCGTTCGCTGCTCGACATGGCGCTCCAGGCGGCGACGTCCTTTCTCCCCTCGGCCCTCTCCGCGCGCAAGGAGGGGTCGGTGAAGGACTCGGCGTCGTTCTTGGGTGTTCGTCTCGCGGCGGATGGCCTCAAGCTGGACACCACCGCTCTGGGCCTACGCACCGTGAGGGTGAGCAGGTCGGCGGACATCCGCGCGCAGACGGCAGCGGTGTCCTCCCCGTCAGTGACCCCCGCGTCGCCGTCTGGCAAGAAGACCCTCCGCAAGGGCACGGCGGTCATCACCGGCGCGTCGTCCGGCCTCGGCCTCGCCACGGCGAAGGCCCTCGCGGAGACAGGCAAGTGGCACGTCATCATGGCCTGCCGCGACTTCCTCAAGGCGTCGCGCGCGGCCAAGGCGGCCGGCATGGACAAGGACAGCTTCACCGTCGTGCACCTGGACCTCGCCTCCCTGGACAGCGTCCGCCAGTTCGTCAGGAACGTGCGCCAGCTGGAGATGCCCATCGACGTGGTGGTCTGCAACGCCGCCGTGTACCAGCCCACCGCCAAGGAGCCGTCCTACACCGCCGACGGCTTCGAGATGAGCGTCGGCGTCAACCACCTCGGCCATTTCCTCCTCGCCCGCGAGCTCCTCAGCGACCTCCAGTCCTCCGACTACCCCTCTAAGCGCCTCATCATCGTCGGCTCCATCACCGCAGGGAACACGAACACGCTGGCGGGGAACGTGCCCCCGAAGGCGAACCTGGGCGACCTGCGCGGCCTCGCCGGCGGCCTCAACGGCGTTGGCAGCTCGGTGATGATCGACGGCGGGGAGTTCGACGGCGCCAAGGCATACAAGGACAGCAAGGTGTGCAACATGCTGACGATGCAGGAGTTCCACCGCCGGTACCACGAGGAGACGGGCGTGACCTTCGCGTCGCTCTACCCGGGCTGCATCGCCACCACGGGCCTGTTCCGCGAGCACATCCCGCTGTTCCGCCTGCTCTTCCCGCCGTTCCAGAAGTACATCACCAAGGGGTACGTCTCCGAGGAGGAGGCCGGGAAGCGGCTGGCGCAGGTGGTGAGCGACCCCAGCCTGACCAAGTCCGGCGTGTACTGGAGCTGGAACAAGAACTCCGCGTCCTTCGAGAACCAGCTCTCTGAGGAGGCCAGCGACGCCGACAAGGCCAAGAAGCTCTGGGAGATCAGCGAGAAGCTCGTCGGCTTGGCGTGATGCCCACCGTGGCCGGCGCCGGCAGCCGGCGACAGTTTTTCCTACCTAGGACATGCTCATTAGTTGGTCTCACTCTCAGTCGAGTAGTCGACGTGTGGTGCGCCCGTGTCAGAGTGCCAAGCCAAGACAGAAAGTGAGTACAAACGTGTAAAATATGTGCTACTGCGACGTGATGGTTTGGGCAGTGGACTGTATGTATCTCTACTCTCTAGGCTTCTGCTTTGCTTTGCATATTTAATTTTCTGCTAATAAGAGGACAGCAAATGCCTTTCGAGCTTCTTTACTTTGTCGGCATTTAGCAGATTCAGATTTTTCTTGAGGACATGGCACGTGAATAATTTCTTCTGTCAGTAGTTCAGTACAAGTTCTCCAATGTTTTTTGTATCCAGCTACTCAAATGTTCCCAGCACAGAATGACACACTTCAATCCATTTCCATTCTCACTGTACACGAAAGTAAACTAGGATAGGGACCTGCATGTTCTAAAAAATATTTCAGATTCAGATTATTTCCAG
CDS Sequence
  • >Zm00001eb220940_T001
    ATGGCGCTCCAGGCGGCGACGTCCTTTCTCCCCTCGGCCCTCTCCGCGCGCAAGGAGGGGTCGGTGAAGGACTCGGCGTCGTTCTTGGGTGTTCGTCTCGCGGCGGATGGCCTCAAGCTGGACACCACCGCTCTGGGCCTACGCACCGTGAGGGTGAGCAGGTCGGCGGACATCCGCGCGCAGACGGCAGCGGTGTCCTCCCCGTCAGTGACCCCCGCGTCGCCGTCTGGCAAGAAGACCCTCCGCAAGGGCACGGCGGTCATCACCGGCGCGTCGTCCGGCCTCGGCCTCGCCACGGCGAAGGCCCTCGCGGAGACAGGCAAGTGGCACGTCATCATGGCCTGCCGCGACTTCCTCAAGGCGTCGCGCGCGGCCAAGGCGGCCGGCATGGACAAGGACAGCTTCACCGTCGTGCACCTGGACCTCGCCTCCCTGGACAGCGTCCGCCAGTTCGTCAGGAACGTGCGCCAGCTGGAGATGCCCATCGACGTGGTGGTCTGCAACGCCGCCGTGTACCAGCCCACCGCCAAGGAGCCGTCCTACACCGCCGACGGCTTCGAGATGAGCGTCGGCGTCAACCACCTCGGCCATTTCCTCCTCGCCCGCGAGCTCCTCAGCGACCTCCAGTCCTCCGACTACCCCTCTAAGCGCCTCATCATCGTCGGCTCCATCACCGCAGGGAACACGAACACGCTGGCGGGGAACGTGCCCCCGAAGGCGAACCTGGGCGACCTGCGCGGCCTCGCCGGCGGCCTCAACGGCGTTGGCAGCTCGGTGATGATCGACGGCGGGGAGTTCGACGGCGCCAAGGCATACAAGGACAGCAAGGTGTGCAACATGCTGACGATGCAGGAGTTCCACCGCCGGTACCACGAGGAGACGGGCGTGACCTTCGCGTCGCTCTACCCGGGCTGCATCGCCACCACGGGCCTGTTCCGCGAGCACATCCCGCTGTTCCGCCTGCTCTTCCCGCCGTTCCAGAAGTACATCACCAAGGGGTACGTCTCCGAGGAGGAGGCCGGGAAGCGGCTGGCGCAGGTGGTGAGCGACCCCAGCCTGACCAAGTCCGGCGTGTACTGGAGCTGGAACAAGAACTCCGCGTCCTTCGAGAACCAGCTCTCTGAGGAGGCCAGCGACGCCGACAAGGCCAAGAAGCTCTGGGAGATCAGCGAGAAGCTCGTCGGCTTGGCGTGA
  • >Zm00001eb220940_T002
    ATGGCGCTCCAGGCGGCGACGTCCTTTCTCCCCTCGGCCCTCTCCGCGCGCAAGGAGGGGTCGGTGAAGGACTCGGCGTCGTTCTTGGGTGTTCGTCTCGCGGCGGATGGCCTCAAGCTGGACACCACCGCTCTGGGCCTACGCACCGTGAGGGTGAGCAGGTCGGCGGACATCCGCGCGCAGACGGCAGCGGTGTCCTCCCCGTCAGTGACCCCCGCGTCGCCGTCTGGCAAGAAGACCCTCCGCAAGGGCACGGCGGTCATCACCGGCGCGTCGTCCGGCCTCGGCCTCGCCACGGCGAAGGCCCTCGCGGAGACAGGCAAGTGGCACGTCATCATGGCCTGCCGCGACTTCCTCAAGGCGTCGCGCGCGGCCAAGGCGGCCGGCATGGACAAGGACAGCTTCACCGTCGTGCACCTGGACCTCGCCTCCCTGGACAGCGTCCGCCAGTTCGTCAGGAACGTGCGCCAGCTGGAGATGCCCATCGACGTGGTGGTCTGCAACGCCGCCGTGTACCAGCCCACCGCCAAGGAGCCGTCCTACACCGCCGACGGCTTCGAGATGAGCGTCGGCGTCAACCACCTCGGCCATTTCCTCCTCGCCCGCGAGCTCCTCAGCGACCTCCAGTCCTCCGACTACCCCTCTAAGCGCCTCATCATCGTCGGCTCCATCACCGCAGGGAACACGAACACGCTGGCGGGGAACGTGCCCCCGAAGGCGAACCTGGGCGACCTGCGCGGCCTCGCCGGCGGCCTCAACGGCGTTGGCAGCTCGGTGATGATCGACGGCGGGGAGTTCGACGGCGCCAAGGCATACAAGGACAGCAAGGTGTGCAACATGCTGACGATGCAGGAGTTCCACCGCCGGTACCACGAGGAGACGGGCGTGACCTTCGCGTCGCTCTACCCGGGCTGCATCGCCACCACGGGCCTGTTCCGCGAGCACATCCCGCTGTTCCGCCTGCTCTTCCCGCCGTTCCAGAAGTACATCACCAAGGGGTACGTCTCCGAGGAGGAGGCCGGGAAGCGGCTGGCGCAGGTGGTGAGCGACCCCAGCCTGACCAAGTCCGGCGTGTACTGGAGCTGGAACAAGAACTCCGCGTCCTTCGAGAACCAGCTCTCTGAGGAGGCCAGCGACGCCGACAAGGCCAAGAAGCTCTGGGAGATCAGCGAGAAGCTCGTCGGCTTGGCGTGA
  • >Zm00001eb220940_T003
    ATGGCGCTCCAGGCGGCGACGTCCTTTCTCCCCTCGGCCCTCTCCGCGCGCAAGGAGGGGTCGGTGAAGGACTCGGCGTCGTTCTTGGGTGTTCGTCTCGCGGCGGATGGCCTCAAGCTGGACACCACCGCTCTGGGCCTACGCACCGTGAGGGTGAGCAGGTCGGCGGACATCCGCGCGCAGACGGCAGCGGTGTCCTCCCCGTCAGTGACCCCCGCGTCGCCGTCTGGCAAGAAGACCCTCCGCAAGGGCACGGCGGTCATCACCGGCGCGTCGTCCGGCCTCGGCCTCGCCACGGCGAAGGCCCTCGCGGAGACAGGCAAGTGGCACGTCATCATGGCCTGCCGCGACTTCCTCAAGGCGTCGCGCGCGGCCAAGGCGGCCGGCATGGACAAGGACAGCTTCACCGTCGTGCACCTGGACCTCGCCTCCCTGGACAGCGTCCGCCAGTTCGTCAGGAACGTGCGCCAGCTGGAGATGCCCATCGACGTGGTGGTCTGCAACGCCGCCGTGTACCAGCCCACCGCCAAGGAGCCGTCCTACACCGCCGACGGCTTCGAGATGAGCGTCGGCGTCAACCACCTCGGCCATTTCCTCCTCGCCCGCGAGCTCCTCAGCGACCTCCAGTCCTCCGACTACCCCTCTAAGCGCCTCATCATCGTCGGCTCCATCACCGCAGGGAACACGAACACGCTGGCGGGGAACGTGCCCCCGAAGGCGAACCTGGGCGACCTGCGCGGCCTCGCCGGCGGCCTCAACGGCGTTGGCAGCTCGGTGATGATCGACGGCGGGGAGTTCGACGGCGCCAAGGCATACAAGGACAGCAAGGTGTGCAACATGCTGACGATGCAGGAGTTCCACCGCCGGTACCACGAGGAGACGGGCGTGACCTTCGCGTCGCTCTACCCGGGCTGCATCGCCACCACGGGCCTGTTCCGCGAGCACATCCCGCTGTTCCGCCTGCTCTTCCCGCCGTTCCAGAAGTACATCACCAAGGGGTACGTCTCCGAGGAGGAGGCCGGGAAGCGGCTGGCGCAGGTGGTGAGCGACCCCAGCCTGACCAAGTCCGGCGTGTACTGGAGCTGGAACAAGAACTCCGCGTCCTTCGAGAACCAGCTCTCTGAGGAGGCCAGCGACGCCGACAAGGCCAAGAAGCTCTGGGAGATCAGCGAGAAGCTCGTCGGCTTGGCGTGA
Protein Sequence
  • >Zm00001eb220940_P001
    MALQAATSFLPSALSARKEGSVKDSASFLGVRLAADGLKLDTTALGLRTVRVSRSADIRAQTAAVSSPSVTPASPSGKKTLRKGTAVITGASSGLGLATAKALAETGKWHVIMACRDFLKASRAAKAAGMDKDSFTVVHLDLASLDSVRQFVRNVRQLEMPIDVVVCNAAVYQPTAKEPSYTADGFEMSVGVNHLGHFLLARELLSDLQSSDYPSKRLIIVGSITAGNTNTLAGNVPPKANLGDLRGLAGGLNGVGSSVMIDGGEFDGAKAYKDSKVCNMLTMQEFHRRYHEETGVTFASLYPGCIATTGLFREHIPLFRLLFPPFQKYITKGYVSEEEAGKRLAQVVSDPSLTKSGVYWSWNKNSASFENQLSEEASDADKAKKLWEISEKLVGLA
  • >Zm00001eb220940_P002
    MALQAATSFLPSALSARKEGSVKDSASFLGVRLAADGLKLDTTALGLRTVRVSRSADIRAQTAAVSSPSVTPASPSGKKTLRKGTAVITGASSGLGLATAKALAETGKWHVIMACRDFLKASRAAKAAGMDKDSFTVVHLDLASLDSVRQFVRNVRQLEMPIDVVVCNAAVYQPTAKEPSYTADGFEMSVGVNHLGHFLLARELLSDLQSSDYPSKRLIIVGSITAGNTNTLAGNVPPKANLGDLRGLAGGLNGVGSSVMIDGGEFDGAKAYKDSKVCNMLTMQEFHRRYHEETGVTFASLYPGCIATTGLFREHIPLFRLLFPPFQKYITKGYVSEEEAGKRLAQVVSDPSLTKSGVYWSWNKNSASFENQLSEEASDADKAKKLWEISEKLVGLA
  • >Zm00001eb220940_P003
    MALQAATSFLPSALSARKEGSVKDSASFLGVRLAADGLKLDTTALGLRTVRVSRSADIRAQTAAVSSPSVTPASPSGKKTLRKGTAVITGASSGLGLATAKALAETGKWHVIMACRDFLKASRAAKAAGMDKDSFTVVHLDLASLDSVRQFVRNVRQLEMPIDVVVCNAAVYQPTAKEPSYTADGFEMSVGVNHLGHFLLARELLSDLQSSDYPSKRLIIVGSITAGNTNTLAGNVPPKANLGDLRGLAGGLNGVGSSVMIDGGEFDGAKAYKDSKVCNMLTMQEFHRRYHEETGVTFASLYPGCIATTGLFREHIPLFRLLFPPFQKYITKGYVSEEEAGKRLAQVVSDPSLTKSGVYWSWNKNSASFENQLSEEASDADKAKKLWEISEKLVGLA

Source: Genetic information comes from the article