Gene Details:

Functional Descriptions:

  • ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize.
  • A maize GMS gene, ZmMs30, which encodes a novel type of GDSL lipase with diverged catalytic residues. Enzyme kinetics and activity assays show that ZmMs30 has lipase activity and prefers to substrates with a short carbon chain.
  • Loss of ZmMs30 function resulted in defective anther cuticle, irregular foot layer of pollen exine, and complete male sterility.
  • Cytological and lipidomics analyses demonstrate that ZmMs30 is crucial for the aliphatic metabolic pathway required for pollen exine formation and anther cuticle development.
  • Male sterility caused by loss of ZmMs30 function was stable in various inbred lines with different genetic background, and that it didn’t show any negative effect on maize heterosis and production, suggesting that ZmMs30 is valuable for cross-breeding and hybrid seed production.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Zm00001eb195900_T002
    AGCGCCGAACGCATGGCGCTCCTCCTCCTCCTCGTCCTGCTCCGCTCCGCCGCCAGCGCTGGCGCGGGCGCTGAACCGCATCGCTCCCCGGCCACTGCCCTCTTCGTCCTGGGCGACTCCACGGTCGGCTGCGCCGCGGCGACGGCGAGCAGCATTCTGTCGCTCAACCTGACCACCACCACCACCACGCTGCCGTCCTCGCTCTCCGGCGAGCCGTGCCTCTTCTTCCACGAGGCGCGGCTCCGCGTCCCGGACCTCCTCGCGGCCAAGATGGGCCTCCCTTCGCCGCCCCCGATCTCCGCGCTCAACGGCACAGCGTCCGCCGCCGCGCGCGGCGTCAACTTCGGCGGCGGCGGCGGGCAGCTGCTGTTCTACGGAGGCGGAGGCGGGGTGCGCGGGCCGGCGTCCGTCTTCCTCACGGGCGCCGTCGGGCAGCAGGTCCGCCTCGCCTCCGAGACGCTGCAGCTGCTGCGGCTCGAGGCCGCCGCGCCGGGGGAGCCGTCGTCCTCGCCCGCCGCAGTGTTCGTCCTGTCCTTCGGCGCCGACGCCTACGCGCGCCTGCTCGCGCGCGGGCCCGCCGAGGCCGCCGCCGCCGCGCCCAAGCACGGCCGCCGCGGGCTCGCCCGCCTCCTCGCCGACCGCGTCGCGCGCGCAGTCTCGGAGCTGTACGAGGCCGGCGCGAGGAGGGTGGCGGTGATGGGGGTGCCGCCGCTGGGGTGCGCGCCGCGCGTCGTGTGGGAGCGGTCGTCCCCCGTCCGCGACGGCGGCGCAGGATGCGTCGAGGAGGCGAACGAGCTGATCCAAGGGTACAACGGCAGGCTCGCGGCGAGGCTGGACGAGCTGCGGCTGGCGCTGGCCGGCGCCGACGTTGTGTTCTGCGACGTGTACAAGGGGATGATGGAGATCATCTCCAACCCGACCACGGTTCGAGGAGACGAGGGAGGCATGCTGCGGGCTGGGGCCCATGAGGGCCACGGTGGGCTGCGTCAGCAAGGAGATGGCGTGCGGCGCGCCGGAGCGCCACGTGTGGTGGGACATCTACAGCCCCACGGAGGCCGCGGATGCCCTCGTGACCAACTGGTCCTGGTCGTCGGACGGCTCCGGCGCCACCAGCATCTGCGGCCCCATCTCTCTGCAGCAGCTGGCTGCAGTGTCGCCGTCGCCGCCGGGGGCGTAGGAACAAACCACTCGTTGTATTTAGTTCACTCGTATTACCCGCCGGAGACCACTCTTATTTAGGAATCTCTTGGCCTATGACTACGGGCGGTGCGTTAAGAGAACCGTCAATGGATATATTGTTTTTCAATGGCCTTTAGCACTGATGGCTCCGAAAAAAAGCTAGTAAGTAGGTTTCCAATCGTC
  • >Zm00001eb195900_T001
    AGCGCCGAACGCATGGCGCTCCTCCTCCTCCTCGTCCTGCTCCGCTCCGCCGCCAGCGCTGGCGCGGGCGCTGAACCGCATCGCTCCCCGGCCACTGCCCTCTTCGTCCTGGGCGACTCCACGGTCGGCTGCGCCGCGGCGACGGCGAGCAGCATTCTGTCGCTCAACCTGACCACCACCACCACCACGCTGCCGTCCTCGCTCTCCGGCGAGCCGTGCCTCTTCTTCCACGAGGCGCGGCTCCGCGTCCCGGACCTCCTCGCGGCCAAGATGGGCCTCCCTTCGCCGCCCCCGATCTCCGCGCTCAACGGCACAGCGTCCGCCGCCGCGCGCGGCGTCAACTTCGGCGGCGGCGGCGGGCAGCTGCTGTTCTACGGAGGCGGAGGCGGGGTGCGCGGGCCGGCGTCCGTCTTCCTCACGGGCGCCGTCGGGCAGCAGGTCCGCCTCGCCTCCGAGACGCTGCAGCTGCTGCGGCTCGAGGCCGCCGCGCCGGGGGAGCCGTCGTCCTCGCCCGCCGCAGTGTTCGTCCTGTCCTTCGGCGCCGACGCCTACGCGCGCCTGCTCGCGCGCGGGCCCGCCGAGGCCGCCGCCGCCGCGCCCAAGCACGGCCGCCGCGGGCTCGCCCGCCTCCTCGCCGACCGCGTCGCGCGCGCAGTCTCGGAGCTGTACGAGGCCGGCGCGAGGAGGGTGGCGGTGATGGGGGTGCCGCCGCTGGGGTGCGCGCCGCGCGTCGTGTGGGAGCGGTCGTCCCCCGTCCGCGACGGCGGCGCAGGATGCGTCGAGGAGGCGAACGAGCTGATCCAAGGGTACAACGGCAGGCTCGCGGCGAGGCTGGACGAGCTGCGGCTGGCGCTGGCCGGCGCCGACGTTGTGTTCTGCGACGTGTACAAGGGGATGATGGAGATCATCTCCAACCCGACCACGGTTCGAGGAGACGAGGGAGGCATGCTGCGGGCTGGGGCCCATGAGGGCCACGGTGGGCTGCGTCAGCAAGGAGATGGCGTGCGGCGCGCCGGAGCGCCACGTGTGGTGGGACATCTACAGCCCCACGGAGGCCGCGGATGCCCTCGTGACCAACTGGTCCTGGTCGTCGGACGGCTCCGGCGCCACCAGCATCTGCGGCCCCATCTCTCTGCAGCAGCTGGCTGCAGTGTCGCCGTCGCCGCCGGGGGCGTAGGAACAAACCACTCGTTGTATTTAGTTCACTCGTATTACCCGCCGGAGACCACTCTTATTTAGGAATCTCTTGGCCTATGACTACGGGCGGTGCGTTAAGAGAACCGTCAATGGATATATTGTTTTTCAATGGCCTTTAGCACTGATGGCTCCGAAAAAAAGCTAGTAAGTAGGTTTCCAATCGTC
CDS Sequence
  • >Zm00001eb195900_T002
    ATGGCGCTCCTCCTCCTCCTCGTCCTGCTCCGCTCCGCCGCCAGCGCTGGCGCGGGCGCTGAACCGCATCGCTCCCCGGCCACTGCCCTCTTCGTCCTGGGCGACTCCACGGTCGGCTGCGCCGCGGCGACGGCGAGCAGCATTCTGTCGCTCAACCTGACCACCACCACCACCACGCTGCCGTCCTCGCTCTCCGGCGAGCCGTGCCTCTTCTTCCACGAGGCGCGGCTCCGCGTCCCGGACCTCCTCGCGGCCAAGATGGGCCTCCCTTCGCCGCCCCCGATCTCCGCGCTCAACGGCACAGCGTCCGCCGCCGCGCGCGGCGTCAACTTCGGCGGCGGCGGCGGGCAGCTGCTGTTCTACGGAGGCGGAGGCGGGGTGCGCGGGCCGGCGTCCGTCTTCCTCACGGGCGCCGTCGGGCAGCAGGTCCGCCTCGCCTCCGAGACGCTGCAGCTGCTGCGGCTCGAGGCCGCCGCGCCGGGGGAGCCGTCGTCCTCGCCCGCCGCAGTGTTCGTCCTGTCCTTCGGCGCCGACGCCTACGCGCGCCTGCTCGCGCGCGGGCCCGCCGAGGCCGCCGCCGCCGCGCCCAAGCACGGCCGCCGCGGGCTCGCCCGCCTCCTCGCCGACCGCGTCGCGCGCGCAGTCTCGGAGCTGTACGAGGCCGGCGCGAGGAGGGTGGCGGTGATGGGGGTGCCGCCGCTGGGGTGCGCGCCGCGCGTCGTGTGGGAGCGGTCGTCCCCCGTCCGCGACGGCGGCGCAGGATGCGTCGAGGAGGCGAACGAGCTGATCCAAGGGTACAACGGCAGGCTCGCGGCGAGGCTGGACGAGCTGCGGCTGGCGCTGGCCGGCGCCGACGTTGTGTTCTGCGACGTGTACAAGGGGATGATGGAGATCATCTCCAACCCGACCACGGTTCGAGGAGACGAGGGAGGCATGCTGCGGGCTGGGGCCCATGAGGGCCACGGTGGGCTGCGTCAGCAAGGAGATGGCGTGCGGCGCGCCGGAGCGCCACGTGTGGTGGGACATCTACAGCCCCACGGAGGCCGCGGATGCCCTCGTGACCAACTGGTCCTGGTCGTCGGACGGCTCCGGCGCCACCAGCATCTGCGGCCCCATCTCTCTGCAGCAGCTGGCTGCAGTGTCGCCGTCGCCGCCGGGGGCGTAGGAACAAACCACTCGTTGTATTTAGTTCACTCGTATTACCCGCCGGAGACCACTCTTATTTAG
  • >Zm00001eb195900_T001
    ATGGCGCTCCTCCTCCTCCTCGTCCTGCTCCGCTCCGCCGCCAGCGCTGGCGCGGGCGCTGAACCGCATCGCTCCCCGGCCACTGCCCTCTTCGTCCTGGGCGACTCCACGGTCGGCTGCGCCGCGGCGACGGCGAGCAGCATTCTGTCGCTCAACCTGACCACCACCACCACCACGCTGCCGTCCTCGCTCTCCGGCGAGCCGTGCCTCTTCTTCCACGAGGCGCGGCTCCGCGTCCCGGACCTCCTCGCGGCCAAGATGGGCCTCCCTTCGCCGCCCCCGATCTCCGCGCTCAACGGCACAGCGTCCGCCGCCGCGCGCGGCGTCAACTTCGGCGGCGGCGGCGGGCAGCTGCTGTTCTACGGAGGCGGAGGCGGGGTGCGCGGGCCGGCGTCCGTCTTCCTCACGGGCGCCGTCGGGCAGCAGGTCCGCCTCGCCTCCGAGACGCTGCAGCTGCTGCGGCTCGAGGCCGCCGCGCCGGGGGAGCCGTCGTCCTCGCCCGCCGCAGTGTTCGTCCTGTCCTTCGGCGCCGACGCCTACGCGCGCCTGCTCGCGCGCGGGCCCGCCGAGGCCGCCGCCGCCGCGCCCAAGCACGGCCGCCGCGGGCTCGCCCGCCTCCTCGCCGACCGCGTCGCGCGCGCAGTCTCGGAGCTGTACGAGGCCGGCGCGAGGAGGGTGGCGGTGATGGGGGTGCCGCCGCTGGGGTGCGCGCCGCGCGTCGTGTGGGAGCGGTCGTCCCCCGTCCGCGACGGCGGCGCAGGATGCGTCGAGGAGGCGAACGAGCTGATCCAAGGGTACAACGGCAGGCTCGCGGCGAGGCTGGACGAGCTGCGGCTGGCGCTGGCCGGCGCCGACGTTGTGTTCTGCGACGTGTACAAGGGGATGATGGAGATCATCTCCAACCCGACCACGGTTCGAGGAGACGAGGGAGGCATGCTGCGGGCTGGGGCCCATGAGGGCCACGGTGGGCTGCGTCAGCAAGGAGATGGCGTGCGGCGCGCCGGAGCGCCACGTGTGGTGGGACATCTACAGCCCCACGGAGGCCGCGGATGCCCTCGTGACCAACTGGTCCTGGTCGTCGGACGGCTCCGGCGCCACCAGCATCTGCGGCCCCATCTCTCTGCAGCAGCTGGCTGCAGTGTCGCCGTCGCCGCCGGGGGCGTAGGAACAAACCACTCGTTGTATTTAGTTCACTCGTATTACCCGCCGGAGACCACTCTTATTTAG
Protein Sequence
  • >Zm00001eb195900_P002
    MALLLLLVLLRSAASAGAGAEPHRSPATALFVLGDSTVGCAAATASSILSLNLTTTTTTLPSSLSGEPCLFFHEARLRVPDLLAAKMGLPSPPPISALNGTASAAARGVNFGGGGGQLLFYGGGGGVRGPASVFLTGAVGQQVRLASETLQLLRLEAAAPGEPSSSPAAVFVLSFGADAYARLLARGPAEAAAAAPKHGRRGLARLLADRVARAVSELYEAGARRVAVMGVPPLGCAPRVVWERSSPVRDGGAGCVEEANELIQGYNGRLAARLDELRLALAGADVVFCDVYKGMMEIISNPTTVRGDEGGMLRAGAHEGHGGLRQQGDGVRRAGAPRVVGHLQPHGGRGCPRDQLVLVVGRLRRHQHLRPHLSAAAGCSVAVAAGGVGTNHSLYLVHSYYPPETTLI
  • >Zm00001eb195900_P001
    MALLLLLVLLRSAASAGAGAEPHRSPATALFVLGDSTVGCAAATASSILSLNLTTTTTTLPSSLSGEPCLFFHEARLRVPDLLAAKMGLPSPPPISALNGTASAAARGVNFGGGGGQLLFYGGGGGVRGPASVFLTGAVGQQVRLASETLQLLRLEAAAPGEPSSSPAAVFVLSFGADAYARLLARGPAEAAAAAPKHGRRGLARLLADRVARAVSELYEAGARRVAVMGVPPLGCAPRVVWERSSPVRDGGAGCVEEANELIQGYNGRLAARLDELRLALAGADVVFCDVYKGMMEIISNPTTVRGDEGGMLRAGAHEGHGGLRQQGDGVRRAGAPRVVGHLQPHGGRGCPRDQLVLVVGRLRRHQHLRPHLSAAAGCSVAVAAGGVGTNHSLYLVHSYYPPETTLI

Source: Genetic information comes from the article