Information report for PGD3
Gene Details
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Functional Descriptions
- The maize chloroplast-localized 6-phosphogluconate dehydrogenase (6PGDH), PGD3, is critical for endosperm starch accumulation.
- We found that cytosolic PGD1 and PGD2 isozymes have heat-stable activity, while amyloplast-localized PGD3 activity is labile under heat stress conditions.
- Transgenic maize plants expressing WPGD1 and WPGD2 with an endosperm-specific promoter increased 6PGDH activity with enhanced heat stability in vitro. WPGD1 and WPGD2 transgenes complement the PGD3-defective kernel phenotype, indicating the fusion proteins are targeted to the amyloplast.
- A small fraction of PGD3 mutants can germinate in tissue culture and complete a full life cycle, indicating PGD3 is primarily essential for kernel development.
Functional Keywords
Literature and News
Gene Resources
- NCBI ID: LOC100856973
- UniProt accessions: B6TX10 , K7TVZ1
- EMBL: CM000780
- AlphaFoldDB: K7TVZ1
- KEGG: zma:100856973
- OMA: QALYMGK
- InterPro: IPR006113 , IPR006114 , IPR006115 , IPR006183 , IPR008927 , IPR013328 , IPR036291
- PANTHER: PTHR11811
- SUPFAM: SSF48179 , SSF51735
- Gene3D: 1.10.1040.10 , 1.20.5.320 , 3.40.50.720
- OrthoDB: K7TVZ1
- SMR: K7TVZ1
Homologs
- Oryza sativa: Os6PGDH2, Os6PGDH;Os6PGDH1
Sequences
cDNA Sequence
- >Zm00001eb169570_T001
GCTGGCCCTTGGACTGATCGACCGGCCAAGCCTGTGCCGTTTGGCCGCCTCGATGAGTCCGCCTCACCGCGAGGCAGCCAGCAGCAGGCGGCTGCGGCCGCGAAAGAACGCAAGAGTGAGGTGGCCCTTTGCAGAAAGCACCTCAAGTCCTCTGGAATTTTTGCTTCCATGAGCGTCGGGTGTACCGGATCCACTCTATTCCTAGGACAGTTTCTACGATAACCCCAGACTCCAGGGACCCCATCCAAAACCCCGGGCCGCAATATTTCTATCCCCTACAGGCTACAGCACTGTCCACCTTCACTTCCGTCCGTTCCCCCGAACTCTCTCTCTCCTCCTCGGCCACCTCCCATCCCCGTTCCTTCCCCTCCATCTCATCACCGCCGCCAGCGCCAAAACCCTACCCACCCACCCACCGATGGCCTCCCCGGCGCCGGCGTCCCCCGCGGCCGCGGCGGCGCACTGCCCCCCGCCGCGCATCGGGCTCGCGGGCCTCGCCACCATGGGCCAGAACCTGGCCCTGAACATCGCCGAGAAAGGGTTCCCGATCTCCGTCTACAACCGCACCGCCGCCAAGGTGGACTCCACGCTGTCCCGCGCGCGGGACGAGGGCTCGCTGCCGGTGCTGGGCCACCGCGACCCGCGCGGGTTCGTGCTGTCCCTCGCGCGCCCGCGCGCCGTCGTGCTGCTCGTCCAGGCCGGCCCCGCCGTCGACGCCACCATCCAGGCCCTCTCCCCCTACCTCGAGCCCGGGGACGCCATCGTCGACGGCGGCAACGAGTGGTACCAGAACACGGAGCGCCGCATCCAGGAGGGCGCGCGGAACGGGCCCTCGCTCATGCCCGGCGGCAGCGCCGACGCCTACGCCAACATCAGGGACATCCTCCAGAAGGCCGCCGCGCAGACGGAGGACGGCGCCTGCGTCACCTTCGTCGGGCCCGGCGGCGCCGGCAACTTCGTCAAGATGGTGCACAACGGGATCGAGTACGGCGACATGCAGCTCATCGCCGAGGCGTACGACGTGCTCCGCAGGCTCGGGGGCCTGTCCAACTCCGAGATCGCCGATGTCTTCGCTGAGTGGAACAGGGGGGAGCTCGAGAGCTTCCTGGTCCAGATCACCGCCGACATTTTCACCGTGGCTGACCCGTTGGACGGGAGCGGGAGTGGCGGCGGGGCGCTGGTTGACAGGATTCTGGACAAGACTGGGATGAAGGGGACCGGGAAATGGACCGTGCAGCAGGCGGCGGAGCTTGCGGTGGCAGCGCCCACGATTGCCGCGTCGCTGGACGGGAGGTACCTGTCAGGGTTGAAGGACGAACGGGTCGCAGCCGCTGGGGTGCTGGAGGAAGAGGGGATGCCGGCGGGCCTGTTGGAGACGGTTAATGTCGACAAGAAGGTGCTGGTGGATAGGGTCAGGCAAGCGCTCTACGCCTCCAAGATTTGCAGCTATGCGCAGGGCATGAATCTGCTGCGAGCCAAGAGCGTGGAGAAGGGATGGAACCTTAACCTCGCAGAGCTTGCCAGGATCTGGAAGGGCGGCTGCATTATCCGTGCGAGGTTCCTTGATAGGATCAAGAGCGCGTACGACAGGAATCCTGAGCTCGCCAATTTGATTGTTGACAGAGAGTTTGCAAGGGAGATGGTGCAGCGGCAGAACGCGTGGAGGTGGGTTGTGGCACGCGCGGTGGAGGCTGGCATTAGCACGCCAGGAATGACTGCTAGCCTTTCGTACTTCGATACCTACAGATCAAGTCGATTGCCTGCAAATCTGATCCAAGCGCAGAGGGATCTGTTTGGTGCACACACCTATGAGCGCATTGACTGTCCGGGTTCATTCCACACCGAATGGACCAAGCTGGCGAGGAGGAGCAATGGCGCAGCCATTTGAGGAATTGGTTGGTATGAGAAGGGATGTTTTCTGCTTTGTGTGATTTCTCTCTGTGGGTTATCTTTTGTTTTACTATTGTTATCTTTATGCTTCTAGATCCAAGTCGAGTACTTCAAATAATGCTGTACTGTATGGTTGGCAAGTGAAGAACATTGTGTAGCTTCAGTTTTACTGTGATGTAGGTACACACAGTTTGATTCTCTTAATTTTGGTATGCAATTGCTATGATGCCTCTCAAACTCCAATGTTGTGTGTTTAAAGAGTTATCTGAAATGTGTTGAAACTGCTACTAAGAGTAGCGAGTTTTGCATCATAGCCTCTCAAATCAATGGTGTCTTTGAAGAATGGTATGCCATGTGTAAAAATAGCTACTACAGTTGTGAAGTCCTAGTGCCCCATTATTGAAGGTAACTTATGAGTCATATATGTTCCTTAAAAAATAATCTTATGTTGATAAACCTAGATGCCTGGGGATCTCTACCTATAAATCAATGATCCTGTCCTGTTTGTTTAAGCAACTTCTCGTGAAAAAGTTATAGTTTTATGCTCGTAGCTTACTGTACCAGCTATGCTAATCAGAAATAACTTTCTGAAATATGCATAATAGTACCCACTCTTCGCCGGAA
CDS Sequence
- >Zm00001eb169570_T001
ATGGCCTCCCCGGCGCCGGCGTCCCCCGCGGCCGCGGCGGCGCACTGCCCCCCGCCGCGCATCGGGCTCGCGGGCCTCGCCACCATGGGCCAGAACCTGGCCCTGAACATCGCCGAGAAAGGGTTCCCGATCTCCGTCTACAACCGCACCGCCGCCAAGGTGGACTCCACGCTGTCCCGCGCGCGGGACGAGGGCTCGCTGCCGGTGCTGGGCCACCGCGACCCGCGCGGGTTCGTGCTGTCCCTCGCGCGCCCGCGCGCCGTCGTGCTGCTCGTCCAGGCCGGCCCCGCCGTCGACGCCACCATCCAGGCCCTCTCCCCCTACCTCGAGCCCGGGGACGCCATCGTCGACGGCGGCAACGAGTGGTACCAGAACACGGAGCGCCGCATCCAGGAGGGCGCGCGGAACGGGCCCTCGCTCATGCCCGGCGGCAGCGCCGACGCCTACGCCAACATCAGGGACATCCTCCAGAAGGCCGCCGCGCAGACGGAGGACGGCGCCTGCGTCACCTTCGTCGGGCCCGGCGGCGCCGGCAACTTCGTCAAGATGGTGCACAACGGGATCGAGTACGGCGACATGCAGCTCATCGCCGAGGCGTACGACGTGCTCCGCAGGCTCGGGGGCCTGTCCAACTCCGAGATCGCCGATGTCTTCGCTGAGTGGAACAGGGGGGAGCTCGAGAGCTTCCTGGTCCAGATCACCGCCGACATTTTCACCGTGGCTGACCCGTTGGACGGGAGCGGGAGTGGCGGCGGGGCGCTGGTTGACAGGATTCTGGACAAGACTGGGATGAAGGGGACCGGGAAATGGACCGTGCAGCAGGCGGCGGAGCTTGCGGTGGCAGCGCCCACGATTGCCGCGTCGCTGGACGGGAGGTACCTGTCAGGGTTGAAGGACGAACGGGTCGCAGCCGCTGGGGTGCTGGAGGAAGAGGGGATGCCGGCGGGCCTGTTGGAGACGGTTAATGTCGACAAGAAGGTGCTGGTGGATAGGGTCAGGCAAGCGCTCTACGCCTCCAAGATTTGCAGCTATGCGCAGGGCATGAATCTGCTGCGAGCCAAGAGCGTGGAGAAGGGATGGAACCTTAACCTCGCAGAGCTTGCCAGGATCTGGAAGGGCGGCTGCATTATCCGTGCGAGGTTCCTTGATAGGATCAAGAGCGCGTACGACAGGAATCCTGAGCTCGCCAATTTGATTGTTGACAGAGAGTTTGCAAGGGAGATGGTGCAGCGGCAGAACGCGTGGAGGTGGGTTGTGGCACGCGCGGTGGAGGCTGGCATTAGCACGCCAGGAATGACTGCTAGCCTTTCGTACTTCGATACCTACAGATCAAGTCGATTGCCTGCAAATCTGATCCAAGCGCAGAGGGATCTGTTTGGTGCACACACCTATGAGCGCATTGACTGTCCGGGTTCATTCCACACCGAATGGACCAAGCTGGCGAGGAGGAGCAATGGCGCAGCCATTTGA
Protein Sequence
- >Zm00001eb169570_P001
MASPAPASPAAAAAHCPPPRIGLAGLATMGQNLALNIAEKGFPISVYNRTAAKVDSTLSRARDEGSLPVLGHRDPRGFVLSLARPRAVVLLVQAGPAVDATIQALSPYLEPGDAIVDGGNEWYQNTERRIQEGARNGPSLMPGGSADAYANIRDILQKAAAQTEDGACVTFVGPGGAGNFVKMVHNGIEYGDMQLIAEAYDVLRRLGGLSNSEIADVFAEWNRGELESFLVQITADIFTVADPLDGSGSGGGALVDRILDKTGMKGTGKWTVQQAAELAVAAPTIAASLDGRYLSGLKDERVAAAGVLEEEGMPAGLLETVNVDKKVLVDRVRQALYASKICSYAQGMNLLRAKSVEKGWNLNLAELARIWKGGCIIRARFLDRIKSAYDRNPELANLIVDREFAREMVQRQNAWRWVVARAVEAGISTPGMTASLSYFDTYRSSRLPANLIQAQRDLFGAHTYERIDCPGSFHTEWTKLARRSNGAAI