Gene Details:

Functional Descriptions:

  • The maize chloroplast-localized 6-phosphogluconate dehydrogenase (6PGDH), PGD3, is critical for endosperm starch accumulation.
  • We found that cytosolic PGD1 and PGD2 isozymes have heat-stable activity, while amyloplast-localized PGD3 activity is labile under heat stress conditions.
  • Transgenic maize plants expressing WPGD1 and WPGD2 with an endosperm-specific promoter increased 6PGDH activity with enhanced heat stability in vitro. WPGD1 and WPGD2 transgenes complement the PGD3-defective kernel phenotype, indicating the fusion proteins are targeted to the amyloplast.
  • A small fraction of PGD3 mutants can germinate in tissue culture and complete a full life cycle, indicating PGD3 is primarily essential for kernel development.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Zm00001eb169570_T001
    GCTGGCCCTTGGACTGATCGACCGGCCAAGCCTGTGCCGTTTGGCCGCCTCGATGAGTCCGCCTCACCGCGAGGCAGCCAGCAGCAGGCGGCTGCGGCCGCGAAAGAACGCAAGAGTGAGGTGGCCCTTTGCAGAAAGCACCTCAAGTCCTCTGGAATTTTTGCTTCCATGAGCGTCGGGTGTACCGGATCCACTCTATTCCTAGGACAGTTTCTACGATAACCCCAGACTCCAGGGACCCCATCCAAAACCCCGGGCCGCAATATTTCTATCCCCTACAGGCTACAGCACTGTCCACCTTCACTTCCGTCCGTTCCCCCGAACTCTCTCTCTCCTCCTCGGCCACCTCCCATCCCCGTTCCTTCCCCTCCATCTCATCACCGCCGCCAGCGCCAAAACCCTACCCACCCACCCACCGATGGCCTCCCCGGCGCCGGCGTCCCCCGCGGCCGCGGCGGCGCACTGCCCCCCGCCGCGCATCGGGCTCGCGGGCCTCGCCACCATGGGCCAGAACCTGGCCCTGAACATCGCCGAGAAAGGGTTCCCGATCTCCGTCTACAACCGCACCGCCGCCAAGGTGGACTCCACGCTGTCCCGCGCGCGGGACGAGGGCTCGCTGCCGGTGCTGGGCCACCGCGACCCGCGCGGGTTCGTGCTGTCCCTCGCGCGCCCGCGCGCCGTCGTGCTGCTCGTCCAGGCCGGCCCCGCCGTCGACGCCACCATCCAGGCCCTCTCCCCCTACCTCGAGCCCGGGGACGCCATCGTCGACGGCGGCAACGAGTGGTACCAGAACACGGAGCGCCGCATCCAGGAGGGCGCGCGGAACGGGCCCTCGCTCATGCCCGGCGGCAGCGCCGACGCCTACGCCAACATCAGGGACATCCTCCAGAAGGCCGCCGCGCAGACGGAGGACGGCGCCTGCGTCACCTTCGTCGGGCCCGGCGGCGCCGGCAACTTCGTCAAGATGGTGCACAACGGGATCGAGTACGGCGACATGCAGCTCATCGCCGAGGCGTACGACGTGCTCCGCAGGCTCGGGGGCCTGTCCAACTCCGAGATCGCCGATGTCTTCGCTGAGTGGAACAGGGGGGAGCTCGAGAGCTTCCTGGTCCAGATCACCGCCGACATTTTCACCGTGGCTGACCCGTTGGACGGGAGCGGGAGTGGCGGCGGGGCGCTGGTTGACAGGATTCTGGACAAGACTGGGATGAAGGGGACCGGGAAATGGACCGTGCAGCAGGCGGCGGAGCTTGCGGTGGCAGCGCCCACGATTGCCGCGTCGCTGGACGGGAGGTACCTGTCAGGGTTGAAGGACGAACGGGTCGCAGCCGCTGGGGTGCTGGAGGAAGAGGGGATGCCGGCGGGCCTGTTGGAGACGGTTAATGTCGACAAGAAGGTGCTGGTGGATAGGGTCAGGCAAGCGCTCTACGCCTCCAAGATTTGCAGCTATGCGCAGGGCATGAATCTGCTGCGAGCCAAGAGCGTGGAGAAGGGATGGAACCTTAACCTCGCAGAGCTTGCCAGGATCTGGAAGGGCGGCTGCATTATCCGTGCGAGGTTCCTTGATAGGATCAAGAGCGCGTACGACAGGAATCCTGAGCTCGCCAATTTGATTGTTGACAGAGAGTTTGCAAGGGAGATGGTGCAGCGGCAGAACGCGTGGAGGTGGGTTGTGGCACGCGCGGTGGAGGCTGGCATTAGCACGCCAGGAATGACTGCTAGCCTTTCGTACTTCGATACCTACAGATCAAGTCGATTGCCTGCAAATCTGATCCAAGCGCAGAGGGATCTGTTTGGTGCACACACCTATGAGCGCATTGACTGTCCGGGTTCATTCCACACCGAATGGACCAAGCTGGCGAGGAGGAGCAATGGCGCAGCCATTTGAGGAATTGGTTGGTATGAGAAGGGATGTTTTCTGCTTTGTGTGATTTCTCTCTGTGGGTTATCTTTTGTTTTACTATTGTTATCTTTATGCTTCTAGATCCAAGTCGAGTACTTCAAATAATGCTGTACTGTATGGTTGGCAAGTGAAGAACATTGTGTAGCTTCAGTTTTACTGTGATGTAGGTACACACAGTTTGATTCTCTTAATTTTGGTATGCAATTGCTATGATGCCTCTCAAACTCCAATGTTGTGTGTTTAAAGAGTTATCTGAAATGTGTTGAAACTGCTACTAAGAGTAGCGAGTTTTGCATCATAGCCTCTCAAATCAATGGTGTCTTTGAAGAATGGTATGCCATGTGTAAAAATAGCTACTACAGTTGTGAAGTCCTAGTGCCCCATTATTGAAGGTAACTTATGAGTCATATATGTTCCTTAAAAAATAATCTTATGTTGATAAACCTAGATGCCTGGGGATCTCTACCTATAAATCAATGATCCTGTCCTGTTTGTTTAAGCAACTTCTCGTGAAAAAGTTATAGTTTTATGCTCGTAGCTTACTGTACCAGCTATGCTAATCAGAAATAACTTTCTGAAATATGCATAATAGTACCCACTCTTCGCCGGAA
CDS Sequence
  • >Zm00001eb169570_T001
    ATGGCCTCCCCGGCGCCGGCGTCCCCCGCGGCCGCGGCGGCGCACTGCCCCCCGCCGCGCATCGGGCTCGCGGGCCTCGCCACCATGGGCCAGAACCTGGCCCTGAACATCGCCGAGAAAGGGTTCCCGATCTCCGTCTACAACCGCACCGCCGCCAAGGTGGACTCCACGCTGTCCCGCGCGCGGGACGAGGGCTCGCTGCCGGTGCTGGGCCACCGCGACCCGCGCGGGTTCGTGCTGTCCCTCGCGCGCCCGCGCGCCGTCGTGCTGCTCGTCCAGGCCGGCCCCGCCGTCGACGCCACCATCCAGGCCCTCTCCCCCTACCTCGAGCCCGGGGACGCCATCGTCGACGGCGGCAACGAGTGGTACCAGAACACGGAGCGCCGCATCCAGGAGGGCGCGCGGAACGGGCCCTCGCTCATGCCCGGCGGCAGCGCCGACGCCTACGCCAACATCAGGGACATCCTCCAGAAGGCCGCCGCGCAGACGGAGGACGGCGCCTGCGTCACCTTCGTCGGGCCCGGCGGCGCCGGCAACTTCGTCAAGATGGTGCACAACGGGATCGAGTACGGCGACATGCAGCTCATCGCCGAGGCGTACGACGTGCTCCGCAGGCTCGGGGGCCTGTCCAACTCCGAGATCGCCGATGTCTTCGCTGAGTGGAACAGGGGGGAGCTCGAGAGCTTCCTGGTCCAGATCACCGCCGACATTTTCACCGTGGCTGACCCGTTGGACGGGAGCGGGAGTGGCGGCGGGGCGCTGGTTGACAGGATTCTGGACAAGACTGGGATGAAGGGGACCGGGAAATGGACCGTGCAGCAGGCGGCGGAGCTTGCGGTGGCAGCGCCCACGATTGCCGCGTCGCTGGACGGGAGGTACCTGTCAGGGTTGAAGGACGAACGGGTCGCAGCCGCTGGGGTGCTGGAGGAAGAGGGGATGCCGGCGGGCCTGTTGGAGACGGTTAATGTCGACAAGAAGGTGCTGGTGGATAGGGTCAGGCAAGCGCTCTACGCCTCCAAGATTTGCAGCTATGCGCAGGGCATGAATCTGCTGCGAGCCAAGAGCGTGGAGAAGGGATGGAACCTTAACCTCGCAGAGCTTGCCAGGATCTGGAAGGGCGGCTGCATTATCCGTGCGAGGTTCCTTGATAGGATCAAGAGCGCGTACGACAGGAATCCTGAGCTCGCCAATTTGATTGTTGACAGAGAGTTTGCAAGGGAGATGGTGCAGCGGCAGAACGCGTGGAGGTGGGTTGTGGCACGCGCGGTGGAGGCTGGCATTAGCACGCCAGGAATGACTGCTAGCCTTTCGTACTTCGATACCTACAGATCAAGTCGATTGCCTGCAAATCTGATCCAAGCGCAGAGGGATCTGTTTGGTGCACACACCTATGAGCGCATTGACTGTCCGGGTTCATTCCACACCGAATGGACCAAGCTGGCGAGGAGGAGCAATGGCGCAGCCATTTGA
Protein Sequence
  • >Zm00001eb169570_P001
    MASPAPASPAAAAAHCPPPRIGLAGLATMGQNLALNIAEKGFPISVYNRTAAKVDSTLSRARDEGSLPVLGHRDPRGFVLSLARPRAVVLLVQAGPAVDATIQALSPYLEPGDAIVDGGNEWYQNTERRIQEGARNGPSLMPGGSADAYANIRDILQKAAAQTEDGACVTFVGPGGAGNFVKMVHNGIEYGDMQLIAEAYDVLRRLGGLSNSEIADVFAEWNRGELESFLVQITADIFTVADPLDGSGSGGGALVDRILDKTGMKGTGKWTVQQAAELAVAAPTIAASLDGRYLSGLKDERVAAAGVLEEEGMPAGLLETVNVDKKVLVDRVRQALYASKICSYAQGMNLLRAKSVEKGWNLNLAELARIWKGGCIIRARFLDRIKSAYDRNPELANLIVDREFAREMVQRQNAWRWVVARAVEAGISTPGMTASLSYFDTYRSSRLPANLIQAQRDLFGAHTYERIDCPGSFHTEWTKLARRSNGAAI

Source: Genetic information comes from the article