Gene Details:

• Gene ID: Zm00001eb121470
• Gene Symbol: ZmC4-NADP-ME|Zmme3
• Gene Name: NADP malic enzyme3
• Genome: Zm-B73-REFERENCE-NAM-5.0
• Species: Zea mays

Functional Descriptions:

• The results clearly show that ZmC4-NADP-ME|Zmme3 is the only one modulated by redox status, and that its oxidation produces a conformational change limiting the catalytic process, although inducing higher affinity binding of the substrates.
• The reversal of ZmC4-NADP-ME|Zmme3 oxidation by chemical reductants suggests the presence of thiol groups able to form disulfide bonds. In order to identify the cysteine residues involved in the activity modulation, site-directed mutagenesis and MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) analysis of ZmC4-NADP-ME|Zmme3 were performed.
• Overall, the results indicate that, besides having acquired a high level of expression and localization in BSCs, ZmC4-NADP-ME|Zmme3 displays a particular redox modulation, which may be required to accomplish the C4 photosynthetic metabolism.
• ZmC4-NADP-ME|Zmme3 displays a particular redox modulation of its activity, which may be required to accomplish the C4 photosynthetic metabolism successfully.

Function-related keywords:

Literature:

• Functional characterization of residues involved in redox modulation of maize photosynthetic NADP-malic enzyme activity.   DOI:  10.1093/pcp/pcs059 ;  PMID:  22514092

Related News:

Gene Resources:

• NCBI ID: LOC542233
• UniProt accessions: P16243

Orthologs:

Sequences:

Source: Genetic information comes from the article