Gene Details:
- Gene ID: Zm00001eb035410
- Gene Symbol: ZmTKPR1-1
- Gene Name: dihydroflavonoid reductase1
- Genome: Zm-B73-REFERENCE-NAM-5.0
- Species: Zea mays
Functional Descriptions:
- CRISPR/Cas9-based genome editing of 14 lipid metabolic genes reveals a sporopollenin metabolon ZmPKSB-ZmTKPR1-1/-2 required for pollen exine formation in maize.
- Identified two maize GMS genes, ZmTKPR1-1 and ZmTKPR1-2, by CRISPR/Cas9 mutagenesis of 14 lipid metabolic genes with anther stage-specific expression patterns.
- ZmTKPR1-1 and ZmTKPR1-2 encode tetraketide α-pyrone reductases and have catalytic activities in reducing tetraketide α-pyrone produced by ZmPKSB (polyketide synthase B).
- ZmTKPR1-1/ZmTKPR1-2 and ZmPKSB formed a protein complex, their mutants showed different, even opposite, defective phenotypes of anther cuticle and pollen exine.
- Based on protein structure prediction, molecular docking, site-directed mutagenesis and biochemical assays, the sporopollenin biosynthetic metabolon ZmPKSB-ZmTKPR1-1/ZmTKPR1-2 was identified to control pollen exine formation in maize anther.
Function-related keywords:
Literature:
- CRISPR/Cas9-based genome editing of 14 lipid metabolic genes reveals a sporopollenin metabolon ZmPKSB-ZmTKPR1-1/-2 required for pollen exine formation in maize. DOI: 10.1111/pbi.14181 ; PMID: 37792967
Related News:
Gene Resources:
- NCBI ID: LOC100273448
- UniProt accessions: C0P4Q8
Orthologs:
Sequences:
cDNA Sequence
- >Zm00001eb035410_T001
ATAAAGACTAAACGGCATGATTAGATGCTAGTTAAAAGTATTAAATAATTTAATTAGTACATGATACGTTCATTCATGTAGTTTCAGACCAAGAGGCTACGCGTGCCGGCGTTCCTCGGCGGCGGGGAGATGGCCATGGCCTATGAACGAGGCGACGGCGTCGTCGAACATCTCCTCGACGCCCTTGAATTCCGCCAGCCCCAGCGCGCGGGCCTTGGACGTGTCGAAGCCGTACGACTGCTCGCCGTAGGCGCGGGGCAGGCTCCTCGGGACGGGGTACGCTGGGAACCGCCGCGCGAGCAGGGCGGCGAGGTCGCCGCAGTCCAGCACCGCCGCGCTGCAGATGTACCTCCCTCGGGCGCCCGCGGCCTCGTACGCCAGCATGTGGCACCTCGCCACGTCGTCGATGTGGACGTACCCCATCCTCCCGCCGAGGACATCAGAAGCGGTGGGGCCGAGCTCAGGAGACAGAGTAGGTCCGACAACGAAGGTCGGAAGAACGGTGACGAGGTCGATCCTGTGCTCTCCGGCGAACTCCCAGGCTGCCTTCTCGGCGAGGATCTTTGCGACGGCGTACCATATCTAAGGGAGCACGGGAACAGCACATTGTCACGCAGGTACGGTCCAAATCACTCTTCTGACGACCGTACGCTCACCTGGAGACTTTCGCAGAACTCGATGGAGCTCCACGACGATTCGTCCAGCAGCACGTTAGGGGGCAGGTCGGCTTCGTCCTTGATCCTCACTGTCGACGACGAGGATGTCAGGACAACCCTCTTGAGCGATGGGTTCTTCTTGCACGAGCGTAGCACGTTCATCGTGCCGTTTATTGCTGAATCAAGCATCTCCTCCTGCCAAGTGGCACCAACATGTTTCAGGTCGCTATATGTCCAGGCCTTTCCTGAAGCTGCAAATATGAGAAACAGAATTGGTGTTTAGCATGCCTTGGAATTAGATCCGGTGACGACGGGCGATGCGGTGTGGAAGACGCCCTCGCAAGCCATCACAGCGTCGTCGAAGCTCCCTTCCTCCAAGAGATCAGCTCTCACGAGCTGCAGCCTTTCCTTTGCGCCTTCCAGGCCCCAGAGGTGCCCTACTTTCTTGCCATTCCCTACCAAGACATCTTTGCTTTCAGGAGTGATTTATTTGACAGGTAATGAAAATGGACATAAACCGTCAACAGGTATTACTAGTATTTTAATTCAGATCATCATCAGAATAAGATGAAACTGACCTGGGTCTCTGACTGTACCCAGGACATGGTACCCAGACTCAAGAAGCCGCTTGACGAGCCAGGAGGCGATGAAACCAGACGCCCCAGTGACACAGACCTTACCCTTAGCAGTGTTAGCCATTCTCTCAGACTCGAGGTGGAGAGTTGGCTTGGTCATGCGCAGATTCCACCACAGCATAGGAGCCTCACAGCTTCTGCATTCCACTGGATTAAACCACTCCCGTCCTACAAAAGTTCACAGCTTAGAAAGGCATGAATTACCAACCTTACATGCATTGCAGGAACCTTCTTCCTTCCTACTGAGACGCTGCTTTATCAAAAATTTCTTAAACATGTGTAATTTCATCACTTCAAGTTACTGGTTAGTGGTCAGTTTTGTTCC - >Zm00001eb035410_T002
ATAAAGACTAAACGGCATGATTAGATGCTAGTTAAAAGTATTAAATAATTTAATTAGTACATGATACGTTCATTCATGTAGTTTCAGACCAAGAGGCTACGCGTGCCGGCGTTCCTCGGCGGCGGGGAGATGGCCATGGCCTATGAACGAGGCGACGGCGTCGTCGAACATCTCCTCGACGCCCTTGAATTCCGCCAGCCCCAGCGCGCGGGCCTTGGACGTGTCGAAGCCGTACGACTGCTCGCCGTAGGCGCGGGGCAGGCTCCTCGGGACGGGGTACGCTGGGAACCGCCGCGCGAGCAGGGCGGCGAGGTCGCCGCAGTCCAGCACCGCCGCGCTGCAGATGTACCTCCCTCGGGCGCCCGCGGCCTCGTACGCCAGCATGTGGCACCTCGCCACGTCGTCGATGTGGACGTACCCCATCCTCCCGCCGAGGACATCAGAAGCGGTGGGGCCGAGCTCAGGAGACAGAGTAGGTCCGACAACGAAGGTCGGAAGAACGGTGACGAGGTCGATCCTGTGCTCTCCGGCGAACTCCCAGGCTGCCTTCTCGGCGAGGATCTTTGCGACGGCGTACCATATCTAAGGGAGCACGGGAACAGCACATTGTCACGCAGGTACGGTCCAAATCACTCTTCTGACGACCGTACGCTCACCTGGAGACTTTCGCAGAACTCGATGGAGCTCCACGACGATTCGTCCAGCAGCACGTTAGGGGGCAGGTCGGCTTCGTCCTTGATCCTCACTGTCGACGACGAGGATGTCAGGACAACCCTCTTGAGCGATGGGTTCTTCTTGCACGAGCGTAGCACGTTCATCGTGCCGTTTATTGCTGAATCAAGCATCTCCTCCTGCCAAGTGGCACCAACATGTTTCAGGTCGCTATATGTCCAGGCCTTTCCTGAAGCTGCAAATATGAGAAACAGAATTGGTGTTTAGCATGCCTTGGAATTAGATCCGGTGACGACGGGCGATGCGGTGTGGAAGACGCCCTCGCAAGCCATCACAGCGTCGTCGAAGCTCCCTTCCTCCAAGAGATCAGCTCTCACGAGCTGCAGCCTTTCCTTTGCGCCTTCCAGGCCCCAGAGGTGCCCTACTTTCTTGCCATTCCCTACCAAGACATCTTTGCTTTCAGGAGTGATTTATTTGACAGGTAATGAAAATGGACATAAACCGTCAACAGGTATTACTAGTATTTTAATTCAGATCATCATCAGAATAAGATGAAACTGACCTGGGTCTCTGACTGTACCCAGGACATGGTACCCAGACTCAAGAAGCCGCTTGACGAGCCAGGAGGCGATGAAACCAGACGCCCCAGTGACACAGACCTTACCCTTAGCAGTGTTAGCCATTCTCTCAGACTCGAGGTGGAGAGTTGGCTTGGTCATGCGCAGATTCCACCACAGCATAGGAGCCTCACAGCTTCTGCATTCCACTGGATTAAACCACTCCCGTCCTACAAAAGTTCACAGCTTAGAAAGGCATGAATTACCAACCTTACATGCATTGCAGGAACCTTCTTCCTTCCTACTGAGACGCTGCTTTATCAAAAATTTCTTAAACATGTGTAATTTCATCACTTCAAGTTACTGGTTAGTGGTCAGTTTTGTTCC
CDS Sequence
- >Zm00001eb035410_T001
ATGGCCATGGCCTATGAACGAGGCGACGGCGTCGTCGAACATCTCCTCGACGCCCTTGAATTCCGCCAGCCCCAGCGCGCGGGCCTTGGACGTGTCGAAGCCGTACGACTGCTCGCCGTAGGCGCGGGGCAGGCTCCTCGGGACGGGGTACGCTGGGAACCGCCGCGCGAGCAGGGCGGCGAGGTCGCCGCAGTCCAGCACCGCCGCGCTGCAGATGTACCTCCCTCGGGCGCCCGCGGCCTCGTACGCCAGCATGTGGCACCTCGCCACGTCGTCGATGTGGACGTACCCCATCCTCCCGCCGAGGACATCAGAAGCGGTGGGGCCGAGCTCAGGAGACAGAGTAGGTCCGACAACGAAGGTCGGAAGAACGGTGACGAGGTCGATCCTGTGCTCTCCGGCGAACTCCCAGGCTGCCTTCTCGGCGAGGATCTTTGCGACGGCGTACCATATCTAAGGGAGCACGGGAACAGCACATTGTCACGCAGGTACGGTCCAAATCACTCTTCTGACGACCGTACGCTCACCTGGAGACTTTCGCAGAACTCGATGGAGCTCCACGACGATTCGTCCAGCAGCACGTTAGGGGGCAGGTCGGCTTCGTCCTTGATCCTCACTGTCGACGACGAGGATGTCAGGACAACCCTCTTGAGCGATGGGTTCTTCTTGCACGAGCGTAGCACGTTCATCGTGCCGTTTATTGCTGAATCAAGCATCTCCTCCTGCCAAGTGGCACCAACATGTTTCAGGTCGCTATATGTCCAGGCCTTTCCTGAAGCTGCAAATATGAGAAACAGAATTGGTGTTTAG - >Zm00001eb035410_T002
ATGGCCATGGCCTATGAACGAGGCGACGGCGTCGTCGAACATCTCCTCGACGCCCTTGAATTCCGCCAGCCCCAGCGCGCGGGCCTTGGACGTGTCGAAGCCGTACGACTGCTCGCCGTAGGCGCGGGGCAGGCTCCTCGGGACGGGGTACGCTGGGAACCGCCGCGCGAGCAGGGCGGCGAGGTCGCCGCAGTCCAGCACCGCCGCGCTGCAGATGTACCTCCCTCGGGCGCCCGCGGCCTCGTACGCCAGCATGTGGCACCTCGCCACGTCGTCGATGTGGACGTACCCCATCCTCCCGCCGAGGACATCAGAAGCGGTGGGGCCGAGCTCAGGAGACAGAGTAGGTCCGACAACGAAGGTCGGAAGAACGGTGACGAGGTCGATCCTGTGCTCTCCGGCGAACTCCCAGGCTGCCTTCTCGGCGAGGATCTTTGCGACGGCGTACCATATCTAAGGGAGCACGGGAACAGCACATTGTCACGCAGGTACGGTCCAAATCACTCTTCTGACGACCGTACGCTCACCTGGAGACTTTCGCAGAACTCGATGGAGCTCCACGACGATTCGTCCAGCAGCACGTTAGGGGGCAGGTCGGCTTCGTCCTTGATCCTCACTGTCGACGACGAGGATGTCAGGACAACCCTCTTGAGCGATGGGTTCTTCTTGCACGAGCGTAGCACGTTCATCGTGCCGTTTATTGCTGAATCAAGCATCTCCTCCTGCCAAGTGGCACCAACATGTTTCAGGTCGCTATATGTCCAGGCCTTTCCTGAAGCTGCAAATATGAGAAACAGAATTGGTGTTTAG
Protein Sequence
- >Zm00001eb035410_P001
MAMAYERGDGVVEHLLDALEFRQPQRAGLGRVEAVRLLAVGAGQAPRDGVRWEPPREQGGEVAAVQHRRAADVPPSGARGLVRQHVAPRHVVDVDVPHPPAEDIRSGGAELRRQSRSDNEGRKNGDEVDPVLSGELPGCLLGEDLCDGVPYLREHGNSTLSRRYGPNHSSDDRTLTWRLSQNSMELHDDSSSSTLGGRSASSLILTVDDEDVRTTLLSDGFFLHERSTFIVPFIAESSISSCQVAPTCFRSLYVQAFPEAANMRNRIGV - >Zm00001eb035410_P002
MAMAYERGDGVVEHLLDALEFRQPQRAGLGRVEAVRLLAVGAGQAPRDGVRWEPPREQGGEVAAVQHRRAADVPPSGARGLVRQHVAPRHVVDVDVPHPPAEDIRSGGAELRRQSRSDNEGRKNGDEVDPVLSGELPGCLLGEDLCDGVPYLREHGNSTLSRRYGPNHSSDDRTLTWRLSQNSMELHDDSSSSTLGGRSASSLILTVDDEDVRTTLLSDGFFLHERSTFIVPFIAESSISSCQVAPTCFRSLYVQAFPEAANMRNRIGV
Source: Genetic information comes from the article