Information report for ZmTKPR1-1
Gene Details
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Functional Descriptions
- CRISPR/Cas9-based genome editing of 14 lipid metabolic genes reveals a sporopollenin metabolon ZmPKSB-ZmTKPR1-1/-2 required for pollen exine formation in maize.
- Identified two maize GMS genes, ZmTKPR1-1 and ZmTKPR1-2, by CRISPR/Cas9 mutagenesis of 14 lipid metabolic genes with anther stage-specific expression patterns.
- ZmTKPR1-1 and ZmTKPR1-2 encode tetraketide α-pyrone reductases and have catalytic activities in reducing tetraketide α-pyrone produced by ZmPKSB (polyketide synthase B).
- ZmTKPR1-1/ZmTKPR1-2 and ZmPKSB formed a protein complex, their mutants showed different, even opposite, defective phenotypes of anther cuticle and pollen exine.
- Based on protein structure prediction, molecular docking, site-directed mutagenesis and biochemical assays, the sporopollenin biosynthetic metabolon ZmPKSB-ZmTKPR1-1/ZmTKPR1-2 was identified to control pollen exine formation in maize anther.
Functional Keywords
Literature and News
Gene Resources
- NCBI ID: LOC100273448
- UniProt accessions: C0P4Q8
- EMBL: BT063277
- AlphaFoldDB: C0P4Q8
Sequences
cDNA Sequence
- >Zm00001eb035410_T001
ATAAAGACTAAACGGCATGATTAGATGCTAGTTAAAAGTATTAAATAATTTAATTAGTACATGATACGTTCATTCATGTAGTTTCAGACCAAGAGGCTACGCGTGCCGGCGTTCCTCGGCGGCGGGGAGATGGCCATGGCCTATGAACGAGGCGACGGCGTCGTCGAACATCTCCTCGACGCCCTTGAATTCCGCCAGCCCCAGCGCGCGGGCCTTGGACGTGTCGAAGCCGTACGACTGCTCGCCGTAGGCGCGGGGCAGGCTCCTCGGGACGGGGTACGCTGGGAACCGCCGCGCGAGCAGGGCGGCGAGGTCGCCGCAGTCCAGCACCGCCGCGCTGCAGATGTACCTCCCTCGGGCGCCCGCGGCCTCGTACGCCAGCATGTGGCACCTCGCCACGTCGTCGATGTGGACGTACCCCATCCTCCCGCCGAGGACATCAGAAGCGGTGGGGCCGAGCTCAGGAGACAGAGTAGGTCCGACAACGAAGGTCGGAAGAACGGTGACGAGGTCGATCCTGTGCTCTCCGGCGAACTCCCAGGCTGCCTTCTCGGCGAGGATCTTTGCGACGGCGTACCATATCTAAGGGAGCACGGGAACAGCACATTGTCACGCAGGTACGGTCCAAATCACTCTTCTGACGACCGTACGCTCACCTGGAGACTTTCGCAGAACTCGATGGAGCTCCACGACGATTCGTCCAGCAGCACGTTAGGGGGCAGGTCGGCTTCGTCCTTGATCCTCACTGTCGACGACGAGGATGTCAGGACAACCCTCTTGAGCGATGGGTTCTTCTTGCACGAGCGTAGCACGTTCATCGTGCCGTTTATTGCTGAATCAAGCATCTCCTCCTGCCAAGTGGCACCAACATGTTTCAGGTCGCTATATGTCCAGGCCTTTCCTGAAGCTGCAAATATGAGAAACAGAATTGGTGTTTAGCATGCCTTGGAATTAGATCCGGTGACGACGGGCGATGCGGTGTGGAAGACGCCCTCGCAAGCCATCACAGCGTCGTCGAAGCTCCCTTCCTCCAAGAGATCAGCTCTCACGAGCTGCAGCCTTTCCTTTGCGCCTTCCAGGCCCCAGAGGTGCCCTACTTTCTTGCCATTCCCTACCAAGACATCTTTGCTTTCAGGAGTGATTTATTTGACAGGTAATGAAAATGGACATAAACCGTCAACAGGTATTACTAGTATTTTAATTCAGATCATCATCAGAATAAGATGAAACTGACCTGGGTCTCTGACTGTACCCAGGACATGGTACCCAGACTCAAGAAGCCGCTTGACGAGCCAGGAGGCGATGAAACCAGACGCCCCAGTGACACAGACCTTACCCTTAGCAGTGTTAGCCATTCTCTCAGACTCGAGGTGGAGAGTTGGCTTGGTCATGCGCAGATTCCACCACAGCATAGGAGCCTCACAGCTTCTGCATTCCACTGGATTAAACCACTCCCGTCCTACAAAAGTTCACAGCTTAGAAAGGCATGAATTACCAACCTTACATGCATTGCAGGAACCTTCTTCCTTCCTACTGAGACGCTGCTTTATCAAAAATTTCTTAAACATGTGTAATTTCATCACTTCAAGTTACTGGTTAGTGGTCAGTTTTGTTCC - >Zm00001eb035410_T002
ATAAAGACTAAACGGCATGATTAGATGCTAGTTAAAAGTATTAAATAATTTAATTAGTACATGATACGTTCATTCATGTAGTTTCAGACCAAGAGGCTACGCGTGCCGGCGTTCCTCGGCGGCGGGGAGATGGCCATGGCCTATGAACGAGGCGACGGCGTCGTCGAACATCTCCTCGACGCCCTTGAATTCCGCCAGCCCCAGCGCGCGGGCCTTGGACGTGTCGAAGCCGTACGACTGCTCGCCGTAGGCGCGGGGCAGGCTCCTCGGGACGGGGTACGCTGGGAACCGCCGCGCGAGCAGGGCGGCGAGGTCGCCGCAGTCCAGCACCGCCGCGCTGCAGATGTACCTCCCTCGGGCGCCCGCGGCCTCGTACGCCAGCATGTGGCACCTCGCCACGTCGTCGATGTGGACGTACCCCATCCTCCCGCCGAGGACATCAGAAGCGGTGGGGCCGAGCTCAGGAGACAGAGTAGGTCCGACAACGAAGGTCGGAAGAACGGTGACGAGGTCGATCCTGTGCTCTCCGGCGAACTCCCAGGCTGCCTTCTCGGCGAGGATCTTTGCGACGGCGTACCATATCTAAGGGAGCACGGGAACAGCACATTGTCACGCAGGTACGGTCCAAATCACTCTTCTGACGACCGTACGCTCACCTGGAGACTTTCGCAGAACTCGATGGAGCTCCACGACGATTCGTCCAGCAGCACGTTAGGGGGCAGGTCGGCTTCGTCCTTGATCCTCACTGTCGACGACGAGGATGTCAGGACAACCCTCTTGAGCGATGGGTTCTTCTTGCACGAGCGTAGCACGTTCATCGTGCCGTTTATTGCTGAATCAAGCATCTCCTCCTGCCAAGTGGCACCAACATGTTTCAGGTCGCTATATGTCCAGGCCTTTCCTGAAGCTGCAAATATGAGAAACAGAATTGGTGTTTAGCATGCCTTGGAATTAGATCCGGTGACGACGGGCGATGCGGTGTGGAAGACGCCCTCGCAAGCCATCACAGCGTCGTCGAAGCTCCCTTCCTCCAAGAGATCAGCTCTCACGAGCTGCAGCCTTTCCTTTGCGCCTTCCAGGCCCCAGAGGTGCCCTACTTTCTTGCCATTCCCTACCAAGACATCTTTGCTTTCAGGAGTGATTTATTTGACAGGTAATGAAAATGGACATAAACCGTCAACAGGTATTACTAGTATTTTAATTCAGATCATCATCAGAATAAGATGAAACTGACCTGGGTCTCTGACTGTACCCAGGACATGGTACCCAGACTCAAGAAGCCGCTTGACGAGCCAGGAGGCGATGAAACCAGACGCCCCAGTGACACAGACCTTACCCTTAGCAGTGTTAGCCATTCTCTCAGACTCGAGGTGGAGAGTTGGCTTGGTCATGCGCAGATTCCACCACAGCATAGGAGCCTCACAGCTTCTGCATTCCACTGGATTAAACCACTCCCGTCCTACAAAAGTTCACAGCTTAGAAAGGCATGAATTACCAACCTTACATGCATTGCAGGAACCTTCTTCCTTCCTACTGAGACGCTGCTTTATCAAAAATTTCTTAAACATGTGTAATTTCATCACTTCAAGTTACTGGTTAGTGGTCAGTTTTGTTCC
CDS Sequence
- >Zm00001eb035410_T001
ATGGCCATGGCCTATGAACGAGGCGACGGCGTCGTCGAACATCTCCTCGACGCCCTTGAATTCCGCCAGCCCCAGCGCGCGGGCCTTGGACGTGTCGAAGCCGTACGACTGCTCGCCGTAGGCGCGGGGCAGGCTCCTCGGGACGGGGTACGCTGGGAACCGCCGCGCGAGCAGGGCGGCGAGGTCGCCGCAGTCCAGCACCGCCGCGCTGCAGATGTACCTCCCTCGGGCGCCCGCGGCCTCGTACGCCAGCATGTGGCACCTCGCCACGTCGTCGATGTGGACGTACCCCATCCTCCCGCCGAGGACATCAGAAGCGGTGGGGCCGAGCTCAGGAGACAGAGTAGGTCCGACAACGAAGGTCGGAAGAACGGTGACGAGGTCGATCCTGTGCTCTCCGGCGAACTCCCAGGCTGCCTTCTCGGCGAGGATCTTTGCGACGGCGTACCATATCTAAGGGAGCACGGGAACAGCACATTGTCACGCAGGTACGGTCCAAATCACTCTTCTGACGACCGTACGCTCACCTGGAGACTTTCGCAGAACTCGATGGAGCTCCACGACGATTCGTCCAGCAGCACGTTAGGGGGCAGGTCGGCTTCGTCCTTGATCCTCACTGTCGACGACGAGGATGTCAGGACAACCCTCTTGAGCGATGGGTTCTTCTTGCACGAGCGTAGCACGTTCATCGTGCCGTTTATTGCTGAATCAAGCATCTCCTCCTGCCAAGTGGCACCAACATGTTTCAGGTCGCTATATGTCCAGGCCTTTCCTGAAGCTGCAAATATGAGAAACAGAATTGGTGTTTAG - >Zm00001eb035410_T002
ATGGCCATGGCCTATGAACGAGGCGACGGCGTCGTCGAACATCTCCTCGACGCCCTTGAATTCCGCCAGCCCCAGCGCGCGGGCCTTGGACGTGTCGAAGCCGTACGACTGCTCGCCGTAGGCGCGGGGCAGGCTCCTCGGGACGGGGTACGCTGGGAACCGCCGCGCGAGCAGGGCGGCGAGGTCGCCGCAGTCCAGCACCGCCGCGCTGCAGATGTACCTCCCTCGGGCGCCCGCGGCCTCGTACGCCAGCATGTGGCACCTCGCCACGTCGTCGATGTGGACGTACCCCATCCTCCCGCCGAGGACATCAGAAGCGGTGGGGCCGAGCTCAGGAGACAGAGTAGGTCCGACAACGAAGGTCGGAAGAACGGTGACGAGGTCGATCCTGTGCTCTCCGGCGAACTCCCAGGCTGCCTTCTCGGCGAGGATCTTTGCGACGGCGTACCATATCTAAGGGAGCACGGGAACAGCACATTGTCACGCAGGTACGGTCCAAATCACTCTTCTGACGACCGTACGCTCACCTGGAGACTTTCGCAGAACTCGATGGAGCTCCACGACGATTCGTCCAGCAGCACGTTAGGGGGCAGGTCGGCTTCGTCCTTGATCCTCACTGTCGACGACGAGGATGTCAGGACAACCCTCTTGAGCGATGGGTTCTTCTTGCACGAGCGTAGCACGTTCATCGTGCCGTTTATTGCTGAATCAAGCATCTCCTCCTGCCAAGTGGCACCAACATGTTTCAGGTCGCTATATGTCCAGGCCTTTCCTGAAGCTGCAAATATGAGAAACAGAATTGGTGTTTAG
Protein Sequence
- >Zm00001eb035410_P001
MAMAYERGDGVVEHLLDALEFRQPQRAGLGRVEAVRLLAVGAGQAPRDGVRWEPPREQGGEVAAVQHRRAADVPPSGARGLVRQHVAPRHVVDVDVPHPPAEDIRSGGAELRRQSRSDNEGRKNGDEVDPVLSGELPGCLLGEDLCDGVPYLREHGNSTLSRRYGPNHSSDDRTLTWRLSQNSMELHDDSSSSTLGGRSASSLILTVDDEDVRTTLLSDGFFLHERSTFIVPFIAESSISSCQVAPTCFRSLYVQAFPEAANMRNRIGV - >Zm00001eb035410_P002
MAMAYERGDGVVEHLLDALEFRQPQRAGLGRVEAVRLLAVGAGQAPRDGVRWEPPREQGGEVAAVQHRRAADVPPSGARGLVRQHVAPRHVVDVDVPHPPAEDIRSGGAELRRQSRSDNEGRKNGDEVDPVLSGELPGCLLGEDLCDGVPYLREHGNSTLSRRYGPNHSSDDRTLTWRLSQNSMELHDDSSSSTLGGRSASSLILTVDDEDVRTTLLSDGFFLHERSTFIVPFIAESSISSCQVAPTCFRSLYVQAFPEAANMRNRIGV