Gene Details:

Functional Descriptions:

  • Identification of a maize (Zea mays) miniature seed6 (Mn6) mutant.
  • Miniature Seed6, encoding an endoplasmic reticulum signal peptidase, is critical in seed development.
  • Mass spectrometric and immunoprecipitation analyses revealed that Mn6 is predominantly involved in processing carbohydrate synthesis-related proteins, including the cell wall invertase miniature seed1 (Mn1).
  • The loss-of-function Mn6 mutant exhibited severely reduced endosperm size.
  • Due to the significant reduction in cell wall invertase activity in the transfer cell layer, mutation of Mn6 caused dramatic defects in endosperm development.
  • These results suggest that proper maturation of Mn1 by Mn6 may be a crucial step for proper seed filling and maize development.
  • Mn6 encodes an endoplasmic reticulum signal peptidase and mutant kernels are relatively small.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Zm00001eb285570_T001
    GCGGGGTCATCTCCGAAAAAAAACGCGAACGCAGAACGCCGCCGGCCCGTCGTGCACTCGTGTTTCCCTTCCTCCACTTGCCTTCGTGTTCGTGATTCGTGATTTCGCCGCCGGAAAGCCGAGGGAGAGGGAGCGATATGGGGTTCGTCGGAGACACGATGGAGTCGATCCGCTCCATGCAGATCCGCCAAGTGCTCATGCAGATCATCAGCCTCGGTATGATTGTTACCTCTGCATTAATCATATGGAAGGGTTTGATAGTTTTCACGGGGAGCGAGTCACCAGTTGTAGTGGTGCTCTCGGGTAGCATGGAGCCTGGATTTAAAAGGGGTGATATCCTGTTTTTGCATATGAGCAAAGATCCTATTCGCACAGGAGAAATAGTTGTTTTCAATGTCGATGGTCGTGAGATTCCAATTGTTCACCGAGTGATTAAGGTCCATGAACGTCAGGACACTGCAGAAGTGGACATCCTCACCAAAGGTGACAATAATTTTGGGGATGACCGACTATTATATGCACATGGCCAGCTTTGGCTCCAGCAACACCACATTATGGGACGTGCGGTGGGCTACCTTCCATATGTTGGCTGGGTTACAATTATCATGACTGAGAAACCATTTATTAAGTACCTGCTGATTGGCGCACTGGGTTTGCTGGTCATAACGTCGAAAGATTAGTGATGTCAAACTTCAGGGCCATGTAGATTTTGCTCTTATGGTGCAACAGCAGAGATCAGTGGCTTTGTGGCGTTTAAGAGATGCCGTTCTGTTGCGCCATGTATCAAACGAGAAGATTTCTTTATCTACTGCGAGGGCACCTGCCTATTGATCGGATAGTCGGTTCTAATCCAGCATCCATCTTTTGTAGTTGTGGCCTAGCTAATGAGTTGAACCAGTCGTTGGTTCTATTCGATGAAGATAACTCTTCATTGCAGTCTTGCAGGAACCTCCTAGGCAATGTGCAATGATAAAAATCTGCGCGGTCTTACGTGCTGCTTCATCATGCTGACTGATGAGCACAACATGCGCCTTTGTTTGTTTCCACTT
CDS Sequence
  • >Zm00001eb285570_T001
    ATGGGGTTCGTCGGAGACACGATGGAGTCGATCCGCTCCATGCAGATCCGCCAAGTGCTCATGCAGATCATCAGCCTCGGTATGATTGTTACCTCTGCATTAATCATATGGAAGGGTTTGATAGTTTTCACGGGGAGCGAGTCACCAGTTGTAGTGGTGCTCTCGGGTAGCATGGAGCCTGGATTTAAAAGGGGTGATATCCTGTTTTTGCATATGAGCAAAGATCCTATTCGCACAGGAGAAATAGTTGTTTTCAATGTCGATGGTCGTGAGATTCCAATTGTTCACCGAGTGATTAAGGTCCATGAACGTCAGGACACTGCAGAAGTGGACATCCTCACCAAAGGTGACAATAATTTTGGGGATGACCGACTATTATATGCACATGGCCAGCTTTGGCTCCAGCAACACCACATTATGGGACGTGCGGTGGGCTACCTTCCATATGTTGGCTGGGTTACAATTATCATGACTGAGAAACCATTTATTAAGTACCTGCTGATTGGCGCACTGGGTTTGCTGGTCATAACGTCGAAAGATTAG
Protein Sequence
  • >Zm00001eb285570_P001
    MGFVGDTMESIRSMQIRQVLMQIISLGMIVTSALIIWKGLIVFTGSESPVVVVLSGSMEPGFKRGDILFLHMSKDPIRTGEIVVFNVDGREIPIVHRVIKVHERQDTAEVDILTKGDNNFGDDRLLYAHGQLWLQQHHIMGRAVGYLPYVGWVTIIMTEKPFIKYLLIGALGLLVITSKD

Source: Genetic information comes from the article