Gene Details:

• Gene ID: Zm00001eb264310
• Gene Symbol: LBL1 rgd1
• Gene Name: ragged seedling1
• Genome: Zm-B73-REFERENCE-NAM-5.0
• Species: Zea mays

Functional Descriptions:

• Here, we address the role of LEAFBLADELESS1 (LBL1), a component of maize trans-acting short-interfering RNA (ta-siRNA), in maize root development. We report that root growth, anatomical patterning, and the number of lateral roots (LRs), monocot-specific crown roots (CRs) and seminal roots (SRs) are significantly affected in LBL1-rgd1 mutant, which is defective in production of ta-siRNA, including tasiR-ARF that targets AUXIN RESPONSE FACTOR3 (ARF3) in maize.
• Thus, LBL1/ta-siRNA module regulates root development, possibly by affecting auxin distribution and signalling, in crosstalk with miR165/166-RLD1/2 module. We further show that ZmLBL1 and its Arabidopsis homologue AtSGS3 proteins are functionally conserved.
• We further studied the expression levels of cell cycle regulators ZmCycB1, ZmCycD2, ZmCycD3 and ZmCycD4, and found that these genes were upregulated in LBL1-rgd1 PR tip (0-1cm) when compared with wild type(Hu et al., 2010). These results suggest that increased cell division activity contributes to the increase in root meristem size, and thus longer PR, in LBL1-rgd1.
• Thus, our results showed that LBL1 is able to rescue the leaf and root developmental defects of sgs3-11, possibly by restoring functional tasiR-ARF production in sgs3-11/- (pAtSGS3:ZmLBL1) plants. This indicates functional conservation of the LBL1- and SGS3-mediated ta-siRNA pathways between dicots and monocots.

Function-related keywords:

• leaf ,  root ,  growth ,  development ,  leaf-development ,  auxin ,  cell-division ,  crown-root-development ,  root-development ,  meristem ,  root-growth ,  auxin-response ,  cell-cycle ,  crown ,  crown-root ,  lateral-root ,  leaf-size ,  root-meristem ,  root-number ,  root-meristem-size ,  leaf-patterning ,  lateral-root-number

Literature:

• Conserved LBL1-ta-siRNA and miR165/166-RLD1/2 modules regulate root development in maize.   DOI:  10.1242/dev.190033 ;  PMID:  33168582

Related News:

• Development | 玉米LBL1-ta-siRNA和miR165/166-RLD1/2 调控根系生长发育

Gene Resources:

• NCBI ID: LOC100037819
• UniProt accessions: A1Y2B7

Sequences:

Source: Genetic information comes from the article