Gene Details:

Functional Descriptions:

  • The results presented here suggest that Kn1 gene could be used as an effective alternative selection marker with a potential to enhance plant transformation efficiency in many plant species.
  • The maize Knotted1 gene is an effective positive selectable marker gene for Agrobacterium-mediated tobacco transformation.
  • Kn1 gene produces similar effects in plant transformation as the ipt gene that is involved in cytokinin biosynthesis.
  • Kn1 gene as a selection marker gene, no antibiotic-resistance or herbicide-resistance genes are needed so that potential risks associated with the use of these traditional selection marker genes can be eliminated.
  • The Maize Transcription Factor KNOTTED1 Directly Regulates the Gibberellin Catabolism Gene ga2ox1.
  • The domains of expression of kn1 and ga2ox1 mRNAs overlap at the base of the shoot apical meristem and the base of newly initiated leaves, suggesting that KN1-mediated activation of ga2ox1 maintains a boundary between meristem cell identity and rapidly elongating cells of the shoot.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Zm00001eb055920_T001
    AGTGCACTGTCTCTCTCCAAAGGCAGCTGCATTGGCCTCCAGCCTTTTCCCTACTGTGCCGCGCGCCCTCCCTTCTCTCTAATGATAGCATAGGGAGAGAAGGCATACTCCGAGGCATCCTTCTCCTTTCCCTCTCCTTCCCCAAACCCTTTTCCTCTTTCCCTCGCCCCAAGAACTTCATCTCATCTCCAGGCGCCCCTTTTGCGCTTGCGCAGGAGGAGCTCACGGGGACAGTGGGCGGAGAGCTCGATCGCTGCACCACTACTTCACTGGAGGTCCGCCCACTCCCATGGAGGAGATCACCCAACACTTTGGAGTTGGCGCAAGCAGCCACGGCCATGGCCACGGCCAGCACCACCATCATCACCACCACCACCACCCGTGGGCATCCTCCCTCAGCGCCGTCGTAGCGCCGCTGCCGCCGCAACCGCCAAGCGCAGGCCTGCCGCTGACCCTGAACACGGTGGCGGCCACTGGGAACAGCGGCGGTAGCGGCAACCCGGTGCTGCAGCTTGCCAACGGTGGCGGCCTCCTCGACGCATGCGTCAAGGCGAAGGAGCCCTCGTCGTCGTCTCCCTACGCAGGCGACGTCGAGGCCATCAAGGCCAAGATCATCTCGCACCCACACTACTACTCGCTCCTCACTGCCTACCTCGAGTGCAACAAGGTGGGGGCACCACCGGAGGTGTCGGCGAGGCTGACGGAGATAGCGCAGGAGGTGGAGGCGCGGCAGCGCACGGCGCTCGGCGGCCTGGCCGCTGCGACGGAGCCGGAGCTGGACCAGTTCATGGAGGCGTACCACGAGATGCTGGTGAAGTTCAGGGAGGAGCTGACGAGGCCGCTGCAGGAGGCGATGGAGTTCATGCGAAGGGTGGAGTCGCAGCTGAACTCGCTTTCCATCTCCGGAAGGTCGCTGCGCAACATCCTTTCATCTGGCTCTTCTGAGGAGGATCAAGAAGGTAGCGGAGGAGAGACCGAGCTCCCTGAAGTTGATGCACATGGTGTGGACCAAGAGCTGAAGCACCATCTCCTGAAGAAATACAGTGGCTATCTAAGCTCGCTCAAGCAAGAACTGTCAAAGAAGAAGAAGAAAGGGAAGCTCCCCAAGGAGGCTCGCCAGCAGCTCCTTAGCTGGTGGGATCAGCACTACAAATGGCCTTACCCCTCAGAGACTCAGAAGGTGGCACTGGCTGAGTCTACCGGGCTTGACCTGAAGCAGATCAACAACTGGTTCATCAACCAGCGGAAGCGGCACTGGAAGCCATCCGAGGAGATGCACCACCTGATGATGGACGGGTACCACACCACCAATGCCTTCTACATGGACGGCCACTTCATCAACGACGGCGGGCTGTACCGGCTCGGCTAGCCACCGGTATCTCGCTTCCATTTCACACCCCACGGCCTAGCTATAAAGACTAATGGTTCCAGGTGTCTGAAGTACTGAAGACAGGGGGGCTAGCTATCTAATGTTTGTGCCGCACGCATGAGCTGTAAGGAGGCCATGCTTAATTATTCTGTTGCCGTTGCTACTCTATCTATATGCGCCTATGCCTCCGTGCATGAACTATGCTTTAGGTGGTTGCTGCTCCACACTGTGGTGGTGTGCTTTTGCTTTTGTGTGGTCGTATTGTATGCGTAACCTGACAGATGGATCCCTGATTGCTACATGTTTGAATAATTTGCATGATCTAGCTAGTTTCTGCCTAATCTAAT
CDS Sequence
  • >Zm00001eb055920_T001
    ATGGAGGAGATCACCCAACACTTTGGAGTTGGCGCAAGCAGCCACGGCCATGGCCACGGCCAGCACCACCATCATCACCACCACCACCACCCGTGGGCATCCTCCCTCAGCGCCGTCGTAGCGCCGCTGCCGCCGCAACCGCCAAGCGCAGGCCTGCCGCTGACCCTGAACACGGTGGCGGCCACTGGGAACAGCGGCGGTAGCGGCAACCCGGTGCTGCAGCTTGCCAACGGTGGCGGCCTCCTCGACGCATGCGTCAAGGCGAAGGAGCCCTCGTCGTCGTCTCCCTACGCAGGCGACGTCGAGGCCATCAAGGCCAAGATCATCTCGCACCCACACTACTACTCGCTCCTCACTGCCTACCTCGAGTGCAACAAGGTGGGGGCACCACCGGAGGTGTCGGCGAGGCTGACGGAGATAGCGCAGGAGGTGGAGGCGCGGCAGCGCACGGCGCTCGGCGGCCTGGCCGCTGCGACGGAGCCGGAGCTGGACCAGTTCATGGAGGCGTACCACGAGATGCTGGTGAAGTTCAGGGAGGAGCTGACGAGGCCGCTGCAGGAGGCGATGGAGTTCATGCGAAGGGTGGAGTCGCAGCTGAACTCGCTTTCCATCTCCGGAAGGTCGCTGCGCAACATCCTTTCATCTGGCTCTTCTGAGGAGGATCAAGAAGGTAGCGGAGGAGAGACCGAGCTCCCTGAAGTTGATGCACATGGTGTGGACCAAGAGCTGAAGCACCATCTCCTGAAGAAATACAGTGGCTATCTAAGCTCGCTCAAGCAAGAACTGTCAAAGAAGAAGAAGAAAGGGAAGCTCCCCAAGGAGGCTCGCCAGCAGCTCCTTAGCTGGTGGGATCAGCACTACAAATGGCCTTACCCCTCAGAGACTCAGAAGGTGGCACTGGCTGAGTCTACCGGGCTTGACCTGAAGCAGATCAACAACTGGTTCATCAACCAGCGGAAGCGGCACTGGAAGCCATCCGAGGAGATGCACCACCTGATGATGGACGGGTACCACACCACCAATGCCTTCTACATGGACGGCCACTTCATCAACGACGGCGGGCTGTACCGGCTCGGCTAG
Protein Sequence
  • >Zm00001eb055920_P001
    MEEITQHFGVGASSHGHGHGQHHHHHHHHHPWASSLSAVVAPLPPQPPSAGLPLTLNTVAATGNSGGSGNPVLQLANGGGLLDACVKAKEPSSSSPYAGDVEAIKAKIISHPHYYSLLTAYLECNKVGAPPEVSARLTEIAQEVEARQRTALGGLAAATEPELDQFMEAYHEMLVKFREELTRPLQEAMEFMRRVESQLNSLSISGRSLRNILSSGSSEEDQEGSGGETELPEVDAHGVDQELKHHLLKKYSGYLSSLKQELSKKKKKGKLPKEARQQLLSWWDQHYKWPYPSETQKVALAESTGLDLKQINNWFINQRKRHWKPSEEMHHLMMDGYHTTNAFYMDGHFINDGGLYRLG

Source: Genetic information comes from the article