Gene Details:

Functional Descriptions:

  • The gene Grassy tillers1 (Gt1), which encodes a homeodomain protein, is expressed in a TB1-dependent manner in response to shade; high Gt1 transcription suppresses the outgrowth of tiller buds and thus decreases tiller number in maize.
  • Here, we used CRISPR-Cas9 gene editing and mutant analysis to ask what conserved roles the homologs of key domestication genes VRS1 and Gt1 have in regulating growth repression in maize and brachypodium.
  • We found that HD-ZIP transcription factors Gt1 and VRL1 regulate growth suppression in branching and flowering structures in maize and brachypodium. Our findings confirm that these two gene lineages share a conserved role in growth suppression and demonstrate how evolution and domestication can be used to predict strong gene editing targets for crop improvement.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Zm00001eb007950_T001
    GACTAGGACACAGCTAACACTCCTGTATCCGAGCTACTACTCCTAGTCCTAGTACAGGCTACAGAGGCAGAGCGAGCAAAACTGCCTGTACATAGCAACCATCAACGACGACCGATCCAAGTAGTGAGCATTAGTGGATCTACTACGTACATATATAGAAGGAAACAGCTGCAGCTCTCTCTCTCGCTGTTTCCTGCGTGATCTGCTTGTGTAGCTACTAGCTAGCTAGCGGAGCGGAACCACCCATGGGCTGCGAGGAGGAAGAGAGGCTGCTGTTCCCGTCCTTCGTCTTTCCCGAGAGCTTCGCGGAGGCCGCCACCCCCGGCTCCGGTGGTGAGCAGAAGAAGGCTCGGCAGCGTCGGAGGCGCAAGCCGCGGCCGGCGGAGGGCGGCGAGGGCGCGGACGAGCAGGCCAGGAAGCGGCGGCTGAGCGACGACCAGGCGCGGTTCCTGGAGCTCAGCTTCAGGAAGGAGCGCAAGCTGGAGACGCCCCGCAAGGTGCAGCTGGCCGCCGAGCTGGGCCTCGACGCCAAGCAGGTGGCCGTCTGGTTCCAGAACCGCCGCGCCCGCCACAAGAGCAAGCTCATGGAGGAGGAGTTCTCCAAGCTCCGCGCCGCGCACGACGCCGTCGTCCTCCACAACTGCCACCTCGAGACCGAGCTGCTGAAGATGAAGGACAGGCTGGCGGAGGTGGAGGAGGAGAAGACGAAGCTCGTCGCGGCGGCGGCGGCGGCGGCCGGCGGCGCAGCGGGCGCAGGGAGCAGCAGCCCGAGCTCGTCGTCGTTCTCGACGGTGACGCACCACCCGGCCGCGGCGCTGCAGGTGGGGCAGTTCGGGGTGGAGCCGGAGGAGGCCGCCGACCTCGCCTACATGACCGAGTACGCCTACAACAGCTACATGAACATGATGGACCTCGCGCCCGCCTACTTCGGTGGAGTCGTCTACGACTACGACCACTTCAACTGATCCACGAGCATGCAGGAGGAGGAGGAGGGAAGCTACAAGACGAAGGAAGGATTGACTGGCCTATCAGCTAGGTACTATTTTCGTGTTAATGGATGGATGGACCGATTTGCAGGGCGCGCGTAGCAGTAGTAGCAATCAAGGTCACTAGTATAGTCTGTCAGTACGACGACGTCGATCAGTCAATGTCTCCTGACGATAATTATTAAGTCGATCTATTCAGTAGTAACTTGTTAAGCCATGTAGATTAGTAGCAGCTGGTAATCTTTGTAAATGTGTGTGGATTATCATGCAAGTGCGCGCTTATCATGATCGAACCAGTGGTCTCTGCGATGTAATATTGGGGGGAACGGGGGTTAATCGGAATCAGAAAATGCATGCTTGTAGATGCTTTATTTGGTG
CDS Sequence
  • >Zm00001eb007950_T001
    ATGGGCTGCGAGGAGGAAGAGAGGCTGCTGTTCCCGTCCTTCGTCTTTCCCGAGAGCTTCGCGGAGGCCGCCACCCCCGGCTCCGGTGGTGAGCAGAAGAAGGCTCGGCAGCGTCGGAGGCGCAAGCCGCGGCCGGCGGAGGGCGGCGAGGGCGCGGACGAGCAGGCCAGGAAGCGGCGGCTGAGCGACGACCAGGCGCGGTTCCTGGAGCTCAGCTTCAGGAAGGAGCGCAAGCTGGAGACGCCCCGCAAGGTGCAGCTGGCCGCCGAGCTGGGCCTCGACGCCAAGCAGGTGGCCGTCTGGTTCCAGAACCGCCGCGCCCGCCACAAGAGCAAGCTCATGGAGGAGGAGTTCTCCAAGCTCCGCGCCGCGCACGACGCCGTCGTCCTCCACAACTGCCACCTCGAGACCGAGCTGCTGAAGATGAAGGACAGGCTGGCGGAGGTGGAGGAGGAGAAGACGAAGCTCGTCGCGGCGGCGGCGGCGGCGGCCGGCGGCGCAGCGGGCGCAGGGAGCAGCAGCCCGAGCTCGTCGTCGTTCTCGACGGTGACGCACCACCCGGCCGCGGCGCTGCAGGTGGGGCAGTTCGGGGTGGAGCCGGAGGAGGCCGCCGACCTCGCCTACATGACCGAGTACGCCTACAACAGCTACATGAACATGATGGACCTCGCGCCCGCCTACTTCGGTGGAGTCGTCTACGACTACGACCACTTCAACTGA
Protein Sequence
  • >Zm00001eb007950_P001
    MGCEEEERLLFPSFVFPESFAEAATPGSGGEQKKARQRRRRKPRPAEGGEGADEQARKRRLSDDQARFLELSFRKERKLETPRKVQLAAELGLDAKQVAVWFQNRRARHKSKLMEEEFSKLRAAHDAVVLHNCHLETELLKMKDRLAEVEEEKTKLVAAAAAAAGGAAGAGSSSPSSSSFSTVTHHPAAALQVGQFGVEPEEAADLAYMTEYAYNSYMNMMDLAPAYFGGVVYDYDHFN

Source: Genetic information comes from the article