Gene Details:

Functional Descriptions:

  • CRISPR–Cas9 to edit maize CLE promoters and a newly characterized maize CLE gene identified through its compensatory upregulation in a ZmCLE7 mutant.
  • This inversion allele led to an expanded expression of ZmCLE7 in developing ear primordia, from the normal epidermal-enriched expression towards the centre of the IM.
  • As ZmCLE7 and ZmFCP1 control meristem size, we observed roughly 5mm stage developing ear primordia in a subset of the alleles.
  • ZmCLE7 promoter-edited alleles as heterozygotes with a null allele increase IM size.
  • At harvest, the fasciated ears of ZmCLE7 null alleles are highly disorganized and shorter with much lower grain yield.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Zm00001eb117820_T002
    TTCACACAGGGGCAGAGGGCGGTCGCGCACACCTTTCCCTTCCCCCCTCTCGTTCCCCTCTCCTTCCCATTAATATCTAGCCGCACCTTCCCTCTCTCACCTCCTCATCCTCCTCCAACTGTGAGCGTACGTTCCACGGCCGCCGCCATTAGTGTTGAGAGAGGGAGAGAAGCTACAGGGACCGGACGCCTCCTTCCTTGCTGACCGGCGAGCTTTATTTTCAGGCCCCTCGCTCGCTAGGGTTCCATCATCCCGCCCGCTGTTCCTTTTCGTCCATGGATCAGAACTTCGGGAACCTCGGGACCGGAGCCGGGAGCAGCGGCGGGTGCGGGGGCTCCAACTCCAACTCCAAGGCGGTGGCGGCGGTGTCGTCCTCGTCCTTCCTGCAGCTGCCGCTGTCGACGGCGGCGGCGGCGTCCCCCGCGTACTACGGCGCGCCGCTCGCGCTCCTGCACCACGGCACCACCGCGGGCCCGTCGTCGTCGCAGCAGCAGCAGCACCAGTCGCCGTACGCCAAGCACGGCGCCGAGATGTCGGCGGCGGAGGCCGACGCCATAAAGGCCAAGATCGTGGCGCACCCGCAGTACTCGGCGCTCCTCGCCGCCTACCTCGACTGCCAGAAAGTGGGCGCGCCGCCGGACCTGCTGGAGAGGCTGACGGCCATGGCGGCGAAGCTGGACGCGCGCCCGCCGGGCCGCCACGGGCCGCGCGACCCGGAGCTGGACCAGTTCATGGAGGCGTACTGCAACATGCTCGTCAAGTACCGGGAGGAGCTGACCCGGCCGATCGACGAGGCCATGGAGTTCCTCAAGCGGGTGGAGGCGCAGCTCGACTCCATCGCCGGCGGCGGCTCCTCCTCCTCCGCGCGCCTCTCGCTCGCCGGCGGCAGCCCAGCGTGGTCGAAACTGACGAGACACTTTGAAATTCGACGACGACAGAACAAAACCTCTGGCTGGAAAGTGGAAACACGTGACCTTACCAAAAGCTCCTCTCTTTTTTTTTTCTTCTTCTTCTGCAGATGGTAAGTCATCAGAAGGGGCCGGCTCTTCGGAAGACGACGACATGGATCCGAGCGGCCGCGAGAACGAGCCGCCGGAGATCGACCCGCGCGCGGAGGACAAGGAGCTCAAGTACCAGCTCCTGAAGAAGTACAGCGGGTACCTGAGCAGCCTGAGGCAGGAGTTCTCCAAGAAGAAGAAGAAAGGGAAGCTGCCGAAGGAGGCCAGGCAGAAGCTCCTCCACTGGTGGGAGCTGCACTACAAGTGGCCGTACCCTTCGGAGACGGAGAAGATCGCGCTGGCAGAGGCGACTGGCCTGGACCAGAAGCAGATCAACAACTGGTTCATCAACCAGCGGAAGCGGCACTGGAAGCCGTCGGAGGACATGCCATTCGTCATGATGGAAGGCTTCCACCCGCAGACCGCGGCCGCGCTGTACCTGGACGGCGGCGCGTTCATGGCCGACGGCATGACGACGTATCGCCTCGGTTCATGAACCGCCGTGGACAATCCAAAACCACCGGGCGACCGTGTCGGAGTGTTTCTGCGTACCAGGAAGATGCGATGCGAGGGACGTTGTTAATTATGGGGGGGTTTTGCTGCCGGTTTCCGCGCTGATCGCCACCAACCAAGGCGGGGTGCGGTCAGTGATCTAGTGGCG
  • >Zm00001eb117820_T001
    TTCACACAGGGGCAGAGGGCGGTCGCGCACACCTTTCCCTTCCCCCCTCTCGTTCCCCTCTCCTTCCCATTAATATCTAGCCGCACCTTCCCTCTCTCACCTCCTCATCCTCCTCCAACTGTGAGCGTACGTTCCACGGCCGCCGCCATTAGTGTTGAGAGAGGGAGAGAAGCTACAGGGACCGGACGCCTCCTTCCTTGCTGACCGGCGAGCTTTATTTTCAGGCCCCTCGCTCGCTAGGGTTCCATCATCCCGCCCGCTGTTCCTTTTCGTCCATGGATCAGAACTTCGGGAACCTCGGGACCGGAGCCGGGAGCAGCGGCGGGTGCGGGGGCTCCAACTCCAACTCCAAGGCGGTGGCGGCGGTGTCGTCCTCGTCCTTCCTGCAGCTGCCGCTGTCGACGGCGGCGGCGGCGTCCCCCGCGTACTACGGCGCGCCGCTCGCGCTCCTGCACCACGGCACCACCGCGGGCCCGTCGTCGTCGCAGCAGCAGCAGCACCAGTCGCCGTACGCCAAGCACGGCGCCGAGATGTCGGCGGCGGAGGCCGACGCCATAAAGGCCAAGATCGTGGCGCACCCGCAGTACTCGGCGCTCCTCGCCGCCTACCTCGACTGCCAGAAAGTGGGCGCGCCGCCGGACCTGCTGGAGAGGCTGACGGCCATGGCGGCGAAGCTGGACGCGCGCCCGCCGGGCCGCCACGGGCCGCGCGACCCGGAGCTGGACCAGTTCATGGAGGCGTACTGCAACATGCTCGTCAAGTACCGGGAGGAGCTGACCCGGCCGATCGACGAGGCCATGGAGTTCCTCAAGCGGGTGGAGGCGCAGCTCGACTCCATCGCCGGCGGCGGCTCCTCCTCCTCCGCGCGCCTCTCGCTCGCCGGCGGCAGCCCAGCGTGGTCGAAACTGACGAGACACTTTGAAATTCGACGACGACAGAACAAAACCTCTGGCTGGAAAGTGGAAACACGTGACCTTACCAAAAGCTCCTCTCTTTTTTTTTTCTTCTTCTTCTGCAGATGGTAAGTCATCAGAAGGGGCCGGCTCTTCGGAAGACGACGACATGGATCCGAGCGGCCGCGAGAACGAGCCGCCGGAGATCGACCCGCGCGCGGAGGACAAGGAGCTCAAGTACCAGCTCCTGAAGAAGTACAGCGGGTACCTGAGCAGCCTGAGGCAGGAGTTCTCCAAGAAGAAGAAGAAAGGGAAGCTGCCGAAGGAGGCCAGGCAGAAGCTCCTCCACTGGTGGGAGCTGCACTACAAGTGGCCGTACCCTTCGGAGACGGAGAAGATCGCGCTGGCAGAGGCGACTGGCCTGGACCAGAAGCAGATCAACAACTGGTTCATCAACCAGCGGAAGCGGCACTGGAAGCCGTCGGAGGACATGCCATTCGTCATGATGGAAGGCTTCCACCCGCAGACCGCGGCCGCGCTGTACCTGGACGGCGGCGCGTTCATGGCCGACGGCATGACGACGTATCGCCTCGGTTCATGAACCGCCGTGGACAATCCAAAACCACCGGGCGACCGTGTCGGAGTGTTTCTGCGTACCAGGAAGATGCGATGCGAGGGACGTTGTTAATTATGGGGGGGTTTTGCTGCCGGTTTCCGCGCTGATCGCCACCAACCAAGGCGGGGTGCGGTCAGTGATCTAGTGGCG
CDS Sequence
  • >Zm00001eb117820_T002
    ATGGATCAGAACTTCGGGAACCTCGGGACCGGAGCCGGGAGCAGCGGCGGGTGCGGGGGCTCCAACTCCAACTCCAAGGCGGTGGCGGCGGTGTCGTCCTCGTCCTTCCTGCAGCTGCCGCTGTCGACGGCGGCGGCGGCGTCCCCCGCGTACTACGGCGCGCCGCTCGCGCTCCTGCACCACGGCACCACCGCGGGCCCGTCGTCGTCGCAGCAGCAGCAGCACCAGTCGCCGTACGCCAAGCACGGCGCCGAGATGTCGGCGGCGGAGGCCGACGCCATAAAGGCCAAGATCGTGGCGCACCCGCAGTACTCGGCGCTCCTCGCCGCCTACCTCGACTGCCAGAAAGTGGGCGCGCCGCCGGACCTGCTGGAGAGGCTGACGGCCATGGCGGCGAAGCTGGACGCGCGCCCGCCGGGCCGCCACGGGCCGCGCGACCCGGAGCTGGACCAGTTCATGGAGGCGTACTGCAACATGCTCGTCAAGTACCGGGAGGAGCTGACCCGGCCGATCGACGAGGCCATGGAGTTCCTCAAGCGGGTGGAGGCGCAGCTCGACTCCATCGCCGGCGGCGGCTCCTCCTCCTCCGCGCGCCTCTCGCTCGCCGGCGGCAGCCCAGCGTGGTCGAAACTGACGAGACACTTTGAAATTCGACGACGACAGAACAAAACCTCTGGCTGGAAAGTGGAAACACGTGACCTTACCAAAAGCTCCTCTCTTTTTTTTTTCTTCTTCTTCTGCAGATGGTAA
  • >Zm00001eb117820_T001
    ATGGATCAGAACTTCGGGAACCTCGGGACCGGAGCCGGGAGCAGCGGCGGGTGCGGGGGCTCCAACTCCAACTCCAAGGCGGTGGCGGCGGTGTCGTCCTCGTCCTTCCTGCAGCTGCCGCTGTCGACGGCGGCGGCGGCGTCCCCCGCGTACTACGGCGCGCCGCTCGCGCTCCTGCACCACGGCACCACCGCGGGCCCGTCGTCGTCGCAGCAGCAGCAGCACCAGTCGCCGTACGCCAAGCACGGCGCCGAGATGTCGGCGGCGGAGGCCGACGCCATAAAGGCCAAGATCGTGGCGCACCCGCAGTACTCGGCGCTCCTCGCCGCCTACCTCGACTGCCAGAAAGTGGGCGCGCCGCCGGACCTGCTGGAGAGGCTGACGGCCATGGCGGCGAAGCTGGACGCGCGCCCGCCGGGCCGCCACGGGCCGCGCGACCCGGAGCTGGACCAGTTCATGGAGGCGTACTGCAACATGCTCGTCAAGTACCGGGAGGAGCTGACCCGGCCGATCGACGAGGCCATGGAGTTCCTCAAGCGGGTGGAGGCGCAGCTCGACTCCATCGCCGGCGGCGGCTCCTCCTCCTCCGCGCGCCTCTCGCTCGCCGGCGGCAGCCCAGCGTGGTCGAAACTGACGAGACACTTTGAAATTCGACGACGACAGAACAAAACCTCTGGCTGGAAAGTGGAAACACGTGACCTTACCAAAAGCTCCTCTCTTTTTTTTTTCTTCTTCTTCTGCAGATGGTAA
Protein Sequence
  • >Zm00001eb117820_P002
    MDQNFGNLGTGAGSSGGCGGSNSNSKAVAAVSSSSFLQLPLSTAAAASPAYYGAPLALLHHGTTAGPSSSQQQQHQSPYAKHGAEMSAAEADAIKAKIVAHPQYSALLAAYLDCQKVGAPPDLLERLTAMAAKLDARPPGRHGPRDPELDQFMEAYCNMLVKYREELTRPIDEAMEFLKRVEAQLDSIAGGGSSSSARLSLAGGSPAWSKLTRHFEIRRRQNKTSGWKVETRDLTKSSSLFFFFFFCRW
  • >Zm00001eb117820_P001
    MDQNFGNLGTGAGSSGGCGGSNSNSKAVAAVSSSSFLQLPLSTAAAASPAYYGAPLALLHHGTTAGPSSSQQQQHQSPYAKHGAEMSAAEADAIKAKIVAHPQYSALLAAYLDCQKVGAPPDLLERLTAMAAKLDARPPGRHGPRDPELDQFMEAYCNMLVKYREELTRPIDEAMEFLKRVEAQLDSIAGGGSSSSARLSLAGGSPAWSKLTRHFEIRRRQNKTSGWKVETRDLTKSSSLFFFFFFCRW

Source: Genetic information comes from the article