Gene Details:

Functional Descriptions:

  • Map-based cloning revealed that the d2014 gene is a novel Br2 allele with a splicing variation, resulting in a higher expression of Br2-T02 instead of normal Br2-T01.
  • Maize brachytic2 (Br2) suppresses the elongation of lower internodes for excessive auxin accumulation in the intercalary meristem region.
  • Br2 encodes a P-glycoprotein1 (PGP1) protein that functions in auxin efflux, and our in situ hybridization assay showed that Br2 was mainly expressed in vascular bundles of the node and internode.
  • The maize Br2 and sorghum orthologue dwarf3 (dw3) exhibit reduced auxin transport and shortened statures, and auxin transport is crucial for regulating internode elongation.
  • The Br2/PGP1-dependent low auxin level in the intercalary meristem region is crucial for internode elongation.
  • Br2/PGP1 transports auxin from node to internode through the vascular bundles.
  • Brachytic2/ZmABCB1 functions in IAA export from intercalary meristems.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Zm00001eb038710_T001
    GTGCCGCATGCATCCATTCCCATTCGTTTTCCACAGTACATGTAGGAGTACTGTGCAAGTAGGGTCCGTACATTCAGTCTCTCTCACTAGTTGGACTCTTCTACTGCTACAAAGACATGAGCTGCCGGGAATGGGAACCGGAGGAGCGAGCGAGCCTGACGGTCTCACACACACAGTCACACTCCCAAGCCAATTATTATAAGAGGCGAGATGAGCAACTCCAGCTCTTAACCAATCCACTCCTCCTCCCTCTCCACCTCCTCTGCTTTGCTCTGCCACTCTGCTGAGGTGGGGGGCAGAGGAGCTCCCCCTCCCTCCTCTCCCCTCCTCGCCATGTCTAGCAGCGACCCGGAGGAGATCAGGGCGCGCGTCGTCGTTCTCGGTTCGCCCCATGCCGACGGCGGCGACGAGTGGGCCCGGCCCGAGCTCGAGGCCTTCCATCTGCCGTCTCCCGCCCACCAGCCTCCTGGCTTCCTAGCCGGGCAACCGGAAGCAGCAGAGCAACCCACGCTCCCTGCTCCTGCTGGCCGCAGCAGCAGCAGCAGCAACACGCCTACTACATCTGCCGGTGGCGGCGCTGCTCCTCCTCCTCCTTCTTCGCCTCCCCCTCCGCCGGCTTCTCTGGAGACCGAGCAGCCGCCCAATGCCAGGCCAGCCTCCGCCGGCGCCAATGACAGCAAGAAGCCCACCCCGCCCGCCGCCCTGCGCGACCTCTTCCGCTTCGCCGACGGCCTCGACTGCGCGCTCATGCTCATCGGCACCCTCGGCGCGCTCGTCCACGGCTGCTCGCTCCCCGTCTTCCTCCGCTTCTTCGCCGACCTCGTCGACTCCTTCGGCTCCCACGCCGACGACCCGGACACCATGGTCCGCCTCGTCGTCAAGTACGCCTTCTACTTCCTCGTCGTCGGAGCGGCAATCTGGGCATCCTCGTGGGCAGAGATCTCTTGCTGGATGTGGACCGGCGAGCGGCAGTCGACGCGGATGCGGATCCGGTACCTGGACGCGGCGCTGCGGCAGGACGTGTCCTTCTTCGACACCGACGTGCGGGCCTCGGACGTGATCTACGCCATCAACGCGGACGCCGTGGTGGTGCAGGACGCCATCAGCGAGAAGCTGGGCAACCTCATCCACTACATGGCCACCTTCGTGGCCGGCTTCGTCGTGGGGTTCACGGCCGCGTGGCAGCTGGCGCTGGTCACGCTGGCCGTGGTGCCGCTCATCGCCGTCATCGGCGGGCTGAGCGCCGCCGCGCTCGCCAAGCTCTCGTCCCGCAGCCAGGACGCGCTCTCGGGCGCCAGCGGCATCGCGGAGCAGGCGCTCGCGCAGATACGGATCGTGCAGGCGTTCGTTGGCGAGGAGCGCGAGATGCGGGCCTACTCGGCGGCGCTGGCCGTGGCGCAGAGGATCGGCTACCGCAGCGGCTTCGCCAAGGGGCTCGGCCTCGGCGGCACCTACTTCACCGTCTTCTGCTGCTACGGGCTCCTGCTCTGGTACGGCGGCCACCTCGTGCGCGCCCAGCACACCAACGGCGGGCTCGCCATCGCCACCATGTTCTCCGTCATGATCGGCGGACTGGCCCTCGGGCAGTCGGCGCCGAGCATGGCCGCGTTCGCCAAGGCGCGTGTGGCGGCTGCCAAGATCTTCCGCATCATCGACCACAGGCCGGGCATCTCCTCGCGCGACGGCGCGGAGCCAGAGTCGGTGACGGGGCGGGTGGAGATGCGGGGCGTGGACTTCGCGTACCCGTCGCGGCCGGACGTCCCCATCCTGCGCGGCTTCTCGCTGAGCGTGCCCGCCGGGAAGACCATCGCGCTGGTGGGCAGCTCCGGCTCCGGGAAGAGCACGGTGGTGTCGCTCATCGAGAGATTCTACGACCCCAGCGCAGGGCAAATCCTGCTGGACGGGCACGACCTCAGGTCGCTGGAGCTGCGGTGGCTGCGGCGGCAGATCGGGCTGGTGAGCCAGGAGCCGGCGCTGTTCGCGACGAGCATCAGGGAGAACCTGCTGCTGGGGCGGGACAGCCAGAGCGCGACGCTGGCGGAGATGGAGGAGGCGGCCAGGGTGGCCAACGCCCACTCCTTCATCATCAAACTCCCCGACGGCTACGACACGCAGGTTGGGGAGCGCGGCCTGCAGCTCTCCGGTGGGCAGAAGCAGCGCATCGCCATCGCCCGCGCCATGCTCAAGAACCCCGCCATCCTGCTGCTGGACGAGGCCACCAGCGCGCTGGACTCCGAGTCTGAGAAGCTCGTGCAGGAGGCGCTGGACCGCTTCATGATCGGGCGCACCACCCTGGTGATCGCGCACAGGCTGTCCACCATCCGCAAGGCCGACGTGGTGGCCGTGCTGCAGGGCGGCGCCGTCTCCGAGATGGGCGCGCACGACGAGCTGATGGCCAAGGGCGAGAACGGCACCTACGCCAAGCTCATCCGCATGCAGGAGCAGGCGCACGAGGCGGCGCTCGTCAACGCCCGCCGCAGCAGCGCCAGGCCCTCCAGCGCCCGCAACTCCGTCAGCTCGCCCATCATGACGCGCAACTCCTCCTACGGCCGCTCCCCCTACTCCCGCCGCCTCTCCGACTTCTCCACCTCCGACTTCACCCTCTCCATCCACGACCCGCACCACCACCACCGGACCATGGCGGACAAGCAGCTGGCGTTCCGCGCCGGCGCCAGCTCCTTCCTGCGCCTCGCCAGGATGAACTCGCCCGAGTGGGCCTACGCGCTCGCCGGCTCCATCGGCTCCATGGTCTGCGGCTCCTTCAGCGCCATCTTCGCCTACATCCTCAGCGCCGTGCTCAGCGTCTACTACGCGCCGGACCCGCGGTACATGAAGCGCGAGATCGCAAAATACTGCTACCTGCTCATCGGCATGTCCTCCGCGGCGCTGCTGTTCAACACGGTGCAGCACGTGTTCTGGGACACGGTGGGCGAGAACCTGACCAAGCGGGTGCGCGAGAAGATGTTCGCCGCCGTGCTCCGCAACGAGATCGCCTGGTTCGACGCGGACGAGAACGCCAGCGCGCGCGTGGCCGCCAGGCTAGCGCTGGACGCCCAGAACGTGCGCTCCGCCATCGGGGACCGCATCTCCGTCATCGTCCAGAACTCGGCGCTGATGCTGGTGGCCTGCACCGCGGGGTTCGTCCTCCAGTGGCGCCTCGCGCTCGTGCTCCTCGCCGTGTTCCCGCTCGTCGTGGGCGCCACCGTGCTGCAGAAGATGTTCATGAAGGGCTTCTCGGGGGACCTGGAGGCCGCGCACGCCAGGGCCACGCAGATCGCGGGCGAGGCCGTGGCCAACCTGCGCACCGTGGCCGCGTTCAACGCGGAGCGCAAGATCACGGGGCTGTTCGAGGCCAACCTGCGCGGCCCGCTCCGGCGCTGCTTCTGGAAGGGGCAGATCGCCGGCAGCGGCTACGGCGTGGCGCAGTTCCTGCTGTACGCGTCCTACGCGCTGGGGCTGTGGTACGCGGCGTGGCTGGTGAAGCACGGCGTGTCCGACTTCTCGCGCACCATCCGCGTGTTCATGGTGCTGATGGTGTCCGCGAACGGCGCCGCCGAGACGCTGACGCTGGCGCCGGACTTCATCAAGGGCGGGCGCGCGATGCGGTCGGTGTTCGAGACGATCGACCGCAAGACGGAGGTGGAGCCCGACGACGTGGACGCGGCGCCGGTGCCGGAGCGGCCGAGGGGCGAGGTGGAGCTGAAGCACGTGGACTTCTCGTACCCGTCGCGGCCGGACATCCAGGTGTTCCGCGACCTGAGCCTCCGTGCGCGCGCCGGGAAGACGCTGGCGCTGGTGGGGCCGAGCGGGTGCGGCAAGAGCTCGGTGCTGGCTCTGGTGCAGCGGTTCTACGAGCCCACGTCCGGGCGCGTGCTCCTGGACGGCAAGGACGTGCGCAAGTACAACCTGCGGGCGCTGCGGCGCGTGGTGGCGGTGGTACCGCAGGAGCCGTTCCTGTTCGCGGCGAGCATCCACGAGAACATCGCGTACGGGCGCGAGGGCGCGACGGAGGCGGAGGTGGTGGAGGCGGCGGCGCAGGCGAACGCGCACCGGTTCATCGCGGCGCTGCCGGAGGGGTACCGGACGCAGGTGGGCGAGCGCGGGGTGCAGCTGTCGGGGGGGCAGCGGCAGCGGATCGCGATCGCGCGCGCGCTGGTGAAGCAGGCGGCCATCGTGCTGCTGGACGAGGCGACCAGCGCGCTGGACGCCGAGTCGGAGCGGTGCGTGCAGGAGGCGCTGGAGCGCGCGGGGTCCGGGCGCACCACCATCGTGGTGGCGCACCGGCTGGCCACGGTGCGCGGCGCGCACACCATCGCGGTCATCGACGACGGCAAGGTGGCGGAGCAGGGGTCGCACTCGCACCTGCTCAAGCACCATCCCGACGGGTGCTACGCGCGGATGCTGCAGCTGCAGCGGCTGACGGGCGCGGCGGCCGGGCCCGGGCCGTCGACCTCGTGCAACGGGGCCGCGTAGGACGGAATGGATGGATGGATGGGTTTGGTTCCTCGAGAGATTGATGGGTGAGGAAGCTGAAGCTCCGGATCAAATGGTGGTACTCCATGATCGCAACAATGAGGGGAAAAAAAGAAAGGAGAAAATACGGTGGTTCATATGATTGTACAATTTGACGATCTGTTTGAGTCGGGGTTTTAGGATGATGTAAACCTTCACTCGCCTTTTTTTTACTCTTGTTTCTCATCCGCATCAGTATCATCTATCTACATACAGTGTCAGAGATGGGAACTGATCCGCATCATCATCTACCTCCCAAGGCACCCCAGATTGTATTAATGTACTTAGTTAGCCTGTTTTATATATACTTATAAGTACC
CDS Sequence
  • >Zm00001eb038710_T001
    ATGTCTAGCAGCGACCCGGAGGAGATCAGGGCGCGCGTCGTCGTTCTCGGTTCGCCCCATGCCGACGGCGGCGACGAGTGGGCCCGGCCCGAGCTCGAGGCCTTCCATCTGCCGTCTCCCGCCCACCAGCCTCCTGGCTTCCTAGCCGGGCAACCGGAAGCAGCAGAGCAACCCACGCTCCCTGCTCCTGCTGGCCGCAGCAGCAGCAGCAGCAACACGCCTACTACATCTGCCGGTGGCGGCGCTGCTCCTCCTCCTCCTTCTTCGCCTCCCCCTCCGCCGGCTTCTCTGGAGACCGAGCAGCCGCCCAATGCCAGGCCAGCCTCCGCCGGCGCCAATGACAGCAAGAAGCCCACCCCGCCCGCCGCCCTGCGCGACCTCTTCCGCTTCGCCGACGGCCTCGACTGCGCGCTCATGCTCATCGGCACCCTCGGCGCGCTCGTCCACGGCTGCTCGCTCCCCGTCTTCCTCCGCTTCTTCGCCGACCTCGTCGACTCCTTCGGCTCCCACGCCGACGACCCGGACACCATGGTCCGCCTCGTCGTCAAGTACGCCTTCTACTTCCTCGTCGTCGGAGCGGCAATCTGGGCATCCTCGTGGGCAGAGATCTCTTGCTGGATGTGGACCGGCGAGCGGCAGTCGACGCGGATGCGGATCCGGTACCTGGACGCGGCGCTGCGGCAGGACGTGTCCTTCTTCGACACCGACGTGCGGGCCTCGGACGTGATCTACGCCATCAACGCGGACGCCGTGGTGGTGCAGGACGCCATCAGCGAGAAGCTGGGCAACCTCATCCACTACATGGCCACCTTCGTGGCCGGCTTCGTCGTGGGGTTCACGGCCGCGTGGCAGCTGGCGCTGGTCACGCTGGCCGTGGTGCCGCTCATCGCCGTCATCGGCGGGCTGAGCGCCGCCGCGCTCGCCAAGCTCTCGTCCCGCAGCCAGGACGCGCTCTCGGGCGCCAGCGGCATCGCGGAGCAGGCGCTCGCGCAGATACGGATCGTGCAGGCGTTCGTTGGCGAGGAGCGCGAGATGCGGGCCTACTCGGCGGCGCTGGCCGTGGCGCAGAGGATCGGCTACCGCAGCGGCTTCGCCAAGGGGCTCGGCCTCGGCGGCACCTACTTCACCGTCTTCTGCTGCTACGGGCTCCTGCTCTGGTACGGCGGCCACCTCGTGCGCGCCCAGCACACCAACGGCGGGCTCGCCATCGCCACCATGTTCTCCGTCATGATCGGCGGACTGGCCCTCGGGCAGTCGGCGCCGAGCATGGCCGCGTTCGCCAAGGCGCGTGTGGCGGCTGCCAAGATCTTCCGCATCATCGACCACAGGCCGGGCATCTCCTCGCGCGACGGCGCGGAGCCAGAGTCGGTGACGGGGCGGGTGGAGATGCGGGGCGTGGACTTCGCGTACCCGTCGCGGCCGGACGTCCCCATCCTGCGCGGCTTCTCGCTGAGCGTGCCCGCCGGGAAGACCATCGCGCTGGTGGGCAGCTCCGGCTCCGGGAAGAGCACGGTGGTGTCGCTCATCGAGAGATTCTACGACCCCAGCGCAGGGCAAATCCTGCTGGACGGGCACGACCTCAGGTCGCTGGAGCTGCGGTGGCTGCGGCGGCAGATCGGGCTGGTGAGCCAGGAGCCGGCGCTGTTCGCGACGAGCATCAGGGAGAACCTGCTGCTGGGGCGGGACAGCCAGAGCGCGACGCTGGCGGAGATGGAGGAGGCGGCCAGGGTGGCCAACGCCCACTCCTTCATCATCAAACTCCCCGACGGCTACGACACGCAGGTTGGGGAGCGCGGCCTGCAGCTCTCCGGTGGGCAGAAGCAGCGCATCGCCATCGCCCGCGCCATGCTCAAGAACCCCGCCATCCTGCTGCTGGACGAGGCCACCAGCGCGCTGGACTCCGAGTCTGAGAAGCTCGTGCAGGAGGCGCTGGACCGCTTCATGATCGGGCGCACCACCCTGGTGATCGCGCACAGGCTGTCCACCATCCGCAAGGCCGACGTGGTGGCCGTGCTGCAGGGCGGCGCCGTCTCCGAGATGGGCGCGCACGACGAGCTGATGGCCAAGGGCGAGAACGGCACCTACGCCAAGCTCATCCGCATGCAGGAGCAGGCGCACGAGGCGGCGCTCGTCAACGCCCGCCGCAGCAGCGCCAGGCCCTCCAGCGCCCGCAACTCCGTCAGCTCGCCCATCATGACGCGCAACTCCTCCTACGGCCGCTCCCCCTACTCCCGCCGCCTCTCCGACTTCTCCACCTCCGACTTCACCCTCTCCATCCACGACCCGCACCACCACCACCGGACCATGGCGGACAAGCAGCTGGCGTTCCGCGCCGGCGCCAGCTCCTTCCTGCGCCTCGCCAGGATGAACTCGCCCGAGTGGGCCTACGCGCTCGCCGGCTCCATCGGCTCCATGGTCTGCGGCTCCTTCAGCGCCATCTTCGCCTACATCCTCAGCGCCGTGCTCAGCGTCTACTACGCGCCGGACCCGCGGTACATGAAGCGCGAGATCGCAAAATACTGCTACCTGCTCATCGGCATGTCCTCCGCGGCGCTGCTGTTCAACACGGTGCAGCACGTGTTCTGGGACACGGTGGGCGAGAACCTGACCAAGCGGGTGCGCGAGAAGATGTTCGCCGCCGTGCTCCGCAACGAGATCGCCTGGTTCGACGCGGACGAGAACGCCAGCGCGCGCGTGGCCGCCAGGCTAGCGCTGGACGCCCAGAACGTGCGCTCCGCCATCGGGGACCGCATCTCCGTCATCGTCCAGAACTCGGCGCTGATGCTGGTGGCCTGCACCGCGGGGTTCGTCCTCCAGTGGCGCCTCGCGCTCGTGCTCCTCGCCGTGTTCCCGCTCGTCGTGGGCGCCACCGTGCTGCAGAAGATGTTCATGAAGGGCTTCTCGGGGGACCTGGAGGCCGCGCACGCCAGGGCCACGCAGATCGCGGGCGAGGCCGTGGCCAACCTGCGCACCGTGGCCGCGTTCAACGCGGAGCGCAAGATCACGGGGCTGTTCGAGGCCAACCTGCGCGGCCCGCTCCGGCGCTGCTTCTGGAAGGGGCAGATCGCCGGCAGCGGCTACGGCGTGGCGCAGTTCCTGCTGTACGCGTCCTACGCGCTGGGGCTGTGGTACGCGGCGTGGCTGGTGAAGCACGGCGTGTCCGACTTCTCGCGCACCATCCGCGTGTTCATGGTGCTGATGGTGTCCGCGAACGGCGCCGCCGAGACGCTGACGCTGGCGCCGGACTTCATCAAGGGCGGGCGCGCGATGCGGTCGGTGTTCGAGACGATCGACCGCAAGACGGAGGTGGAGCCCGACGACGTGGACGCGGCGCCGGTGCCGGAGCGGCCGAGGGGCGAGGTGGAGCTGAAGCACGTGGACTTCTCGTACCCGTCGCGGCCGGACATCCAGGTGTTCCGCGACCTGAGCCTCCGTGCGCGCGCCGGGAAGACGCTGGCGCTGGTGGGGCCGAGCGGGTGCGGCAAGAGCTCGGTGCTGGCTCTGGTGCAGCGGTTCTACGAGCCCACGTCCGGGCGCGTGCTCCTGGACGGCAAGGACGTGCGCAAGTACAACCTGCGGGCGCTGCGGCGCGTGGTGGCGGTGGTACCGCAGGAGCCGTTCCTGTTCGCGGCGAGCATCCACGAGAACATCGCGTACGGGCGCGAGGGCGCGACGGAGGCGGAGGTGGTGGAGGCGGCGGCGCAGGCGAACGCGCACCGGTTCATCGCGGCGCTGCCGGAGGGGTACCGGACGCAGGTGGGCGAGCGCGGGGTGCAGCTGTCGGGGGGGCAGCGGCAGCGGATCGCGATCGCGCGCGCGCTGGTGAAGCAGGCGGCCATCGTGCTGCTGGACGAGGCGACCAGCGCGCTGGACGCCGAGTCGGAGCGGTGCGTGCAGGAGGCGCTGGAGCGCGCGGGGTCCGGGCGCACCACCATCGTGGTGGCGCACCGGCTGGCCACGGTGCGCGGCGCGCACACCATCGCGGTCATCGACGACGGCAAGGTGGCGGAGCAGGGGTCGCACTCGCACCTGCTCAAGCACCATCCCGACGGGTGCTACGCGCGGATGCTGCAGCTGCAGCGGCTGACGGGCGCGGCGGCCGGGCCCGGGCCGTCGACCTCGTGCAACGGGGCCGCGTAG
Protein Sequence
  • >Zm00001eb038710_P001
    MSSSDPEEIRARVVVLGSPHADGGDEWARPELEAFHLPSPAHQPPGFLAGQPEAAEQPTLPAPAGRSSSSSNTPTTSAGGGAAPPPPSSPPPPPASLETEQPPNARPASAGANDSKKPTPPAALRDLFRFADGLDCALMLIGTLGALVHGCSLPVFLRFFADLVDSFGSHADDPDTMVRLVVKYAFYFLVVGAAIWASSWAEISCWMWTGERQSTRMRIRYLDAALRQDVSFFDTDVRASDVIYAINADAVVVQDAISEKLGNLIHYMATFVAGFVVGFTAAWQLALVTLAVVPLIAVIGGLSAAALAKLSSRSQDALSGASGIAEQALAQIRIVQAFVGEEREMRAYSAALAVAQRIGYRSGFAKGLGLGGTYFTVFCCYGLLLWYGGHLVRAQHTNGGLAIATMFSVMIGGLALGQSAPSMAAFAKARVAAAKIFRIIDHRPGISSRDGAEPESVTGRVEMRGVDFAYPSRPDVPILRGFSLSVPAGKTIALVGSSGSGKSTVVSLIERFYDPSAGQILLDGHDLRSLELRWLRRQIGLVSQEPALFATSIRENLLLGRDSQSATLAEMEEAARVANAHSFIIKLPDGYDTQVGERGLQLSGGQKQRIAIARAMLKNPAILLLDEATSALDSESEKLVQEALDRFMIGRTTLVIAHRLSTIRKADVVAVLQGGAVSEMGAHDELMAKGENGTYAKLIRMQEQAHEAALVNARRSSARPSSARNSVSSPIMTRNSSYGRSPYSRRLSDFSTSDFTLSIHDPHHHHRTMADKQLAFRAGASSFLRLARMNSPEWAYALAGSIGSMVCGSFSAIFAYILSAVLSVYYAPDPRYMKREIAKYCYLLIGMSSAALLFNTVQHVFWDTVGENLTKRVREKMFAAVLRNEIAWFDADENASARVAARLALDAQNVRSAIGDRISVIVQNSALMLVACTAGFVLQWRLALVLLAVFPLVVGATVLQKMFMKGFSGDLEAAHARATQIAGEAVANLRTVAAFNAERKITGLFEANLRGPLRRCFWKGQIAGSGYGVAQFLLYASYALGLWYAAWLVKHGVSDFSRTIRVFMVLMVSANGAAETLTLAPDFIKGGRAMRSVFETIDRKTEVEPDDVDAAPVPERPRGEVELKHVDFSYPSRPDIQVFRDLSLRARAGKTLALVGPSGCGKSSVLALVQRFYEPTSGRVLLDGKDVRKYNLRALRRVVAVVPQEPFLFAASIHENIAYGREGATEAEVVEAAAQANAHRFIAALPEGYRTQVGERGVQLSGGQRQRIAIARALVKQAAIVLLDEATSALDAESERCVQEALERAGSGRTTIVVAHRLATVRGAHTIAVIDDGKVAEQGSHSHLLKHHPDGCYARMLQLQRLTGAAAGPGPSTSCNGAA

Source: Genetic information comes from the article