Gene Details:

Functional Descriptions:

  • Two shikimate dehydrogenases, VvSDH3 and VvSDH4, are involved in gallic acid biosynthesis in grapevine.
  • VvSDH3 and VvSDH4, mainly expressed in immature berry tissues in which galloylated flavan-3-ols are accumulated, encoded enzymes with lower ‘classical’ activity but were able to produce gallic acid in vitro.
  • The over-expression of VvSDH3 in hairy-roots increased the content of aromatic amino acids and hydroxycinnamates, but had little or no effect on molecules more distant from the shikimate pathway (stilbenoids and flavan-3-ols).
  • VvSDH3 and VvSDH4 are good candidates for GA biosynthesis in immature grape berry tissues. However, other molecular actors involved in GA transport and PA galloylation remain to be identified.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Vitvi14g00339_t001
    TTTCCTTCTTTCAGGCTTTCAGCAGCTTCTAACTAAGCCACTGTCCACTGAATATTTTTCGATATGGGGAGCCTCCCATTTACTGTTTCAGATCTTCAGACAAGTGTTAGTGGAGTTAGGAGCAACCCCACTTTGCTATGCACCCCATTGATGGGTACCACAGTGGAGCAGATGCTGACCGAGATGAGAAAAGCAAAGGAAATTGGTGCAGATATTGTGGAAATCAGGCTGGATTGTTTGAGGAACTTCAGTCCTGCACAAGATCTTCAAATTCTTATCAAACAATCTCCTTTGCCAACTCTAGTGACTTATAGACCTATATGGGAGGGTGGTCAGTATGAAGGTGATGAAAACAAGCGACAAGATGCACTTCGACTCGCAATGGAACTGGGTGCAAGTTACATTGATGTTGAGCTTGAGGTTGCTCATGAATTCAATAATTCAATTTATGGGAAGAAGCCCCAAAACTTCAAAGTCATTGTTTCTTCTCACAATTTCCACAACACCCCATCTACTGAGGCTATTGGGAATCTGGTGGCCAGAATCCAAGCCAGTGGAGCTGATATAGTGAAGATTGCAACAACTGCATTAGACATCACAGATGTGGCTCGGGTTCTTCAAGTAACTGTGCATTCTCAAGTTCCAACAATAGCAATTGTCATGGGGGAGAGGGGTTTGATTTCACGGTTACTTAGCCCCAAATTTGGTGGATATCTCACTTATGGTGCACTTGAAGCTGGTGCAATATCAGCTCCTGGGCAACCAACAGCCAAAGACTTGTTGGATTTGTACAACTTCAGGCTTATTAAACCCGATACCAAAGTATATGGCATCATTGGGAAGCCTGTTGGACACAGCAAGAGTCCTCTTTTGTTCAATGCAGCATTTAAGAAAGTCGGTCTTAATGCTGTTTATGTGCATTTGCTGGTGGATGATGTTGAGAAGTTCTTCAACACCTATTCAGCCCCTGATTTTATCTCTGGATGCAGTTGTACAATCCCTCACAAGGAGGTTGCAATCAAATGCATGGATACTATTGACCCCATTGCACGGAAAATTGGAGCTATTAACAATATTGTTAGGAAACCAGATGGAAAGTTAACTGCTTTTAACACCGACTACATTGGTGCCATAGAAGCCATAGAGGATGGACTAAGAGAATCAAATGGTTCAAGCCCTGCAGTTGGCTCACCCTTAGCCGGGAAATTGTTCGTTGTCTTGGGAGCTGGTGGTGCTGGCAAGTCACTCGCTTATGGTGCTAAAGAAAAGGGAGCAAGAGTGGTAGTTGCCAACCGAACATTTGAAAGAGCTAAGGATCTAGCTGACAAAGTTGGGGGCCAAGCTTTGACCCTTGCAGAAATAGAGAACTTCCACCCAGAAGAAGGGATGATTCTTGCAAACACTACATCTGTGGGAATGAAACCGAAAATTAATGACACTCCCATTCCCAAGCATGCTTTGAAGCATTACTCCCTAGTATTTGATGCCATATACACACCAAAAGACACTAGACTCTTGAGGGAAGCAAAAGAGAGTGGAAAAATTATAGTTTATGGGACAGAGATGTTGATCCGCCAAGGCTTTGAACAATACAAGAACTTCACTGGTTTGCCTGCCCCTGAAGAATTGTTTAGGGAGCTTATGTCGAAACACGCATAAGAGGATATTTGCTTGGGCAGTCAGTTGTTGCAGCTGGATCTATGTTTATACCTGCCTCATTTCAGTTCCTTACCATTTGGTTAGCTATATATTC
CDS Sequence
  • >Vitvi14g00339_t001
    ATGGGGAGCCTCCCATTTACTGTTTCAGATCTTCAGACAAGTGTTAGTGGAGTTAGGAGCAACCCCACTTTGCTATGCACCCCATTGATGGGTACCACAGTGGAGCAGATGCTGACCGAGATGAGAAAAGCAAAGGAAATTGGTGCAGATATTGTGGAAATCAGGCTGGATTGTTTGAGGAACTTCAGTCCTGCACAAGATCTTCAAATTCTTATCAAACAATCTCCTTTGCCAACTCTAGTGACTTATAGACCTATATGGGAGGGTGGTCAGTATGAAGGTGATGAAAACAAGCGACAAGATGCACTTCGACTCGCAATGGAACTGGGTGCAAGTTACATTGATGTTGAGCTTGAGGTTGCTCATGAATTCAATAATTCAATTTATGGGAAGAAGCCCCAAAACTTCAAAGTCATTGTTTCTTCTCACAATTTCCACAACACCCCATCTACTGAGGCTATTGGGAATCTGGTGGCCAGAATCCAAGCCAGTGGAGCTGATATAGTGAAGATTGCAACAACTGCATTAGACATCACAGATGTGGCTCGGGTTCTTCAAGTAACTGTGCATTCTCAAGTTCCAACAATAGCAATTGTCATGGGGGAGAGGGGTTTGATTTCACGGTTACTTAGCCCCAAATTTGGTGGATATCTCACTTATGGTGCACTTGAAGCTGGTGCAATATCAGCTCCTGGGCAACCAACAGCCAAAGACTTGTTGGATTTGTACAACTTCAGGCTTATTAAACCCGATACCAAAGTATATGGCATCATTGGGAAGCCTGTTGGACACAGCAAGAGTCCTCTTTTGTTCAATGCAGCATTTAAGAAAGTCGGTCTTAATGCTGTTTATGTGCATTTGCTGGTGGATGATGTTGAGAAGTTCTTCAACACCTATTCAGCCCCTGATTTTATCTCTGGATGCAGTTGTACAATCCCTCACAAGGAGGTTGCAATCAAATGCATGGATACTATTGACCCCATTGCACGGAAAATTGGAGCTATTAACAATATTGTTAGGAAACCAGATGGAAAGTTAACTGCTTTTAACACCGACTACATTGGTGCCATAGAAGCCATAGAGGATGGACTAAGAGAATCAAATGGTTCAAGCCCTGCAGTTGGCTCACCCTTAGCCGGGAAATTGTTCGTTGTCTTGGGAGCTGGTGGTGCTGGCAAGTCACTCGCTTATGGTGCTAAAGAAAAGGGAGCAAGAGTGGTAGTTGCCAACCGAACATTTGAAAGAGCTAAGGATCTAGCTGACAAAGTTGGGGGCCAAGCTTTGACCCTTGCAGAAATAGAGAACTTCCACCCAGAAGAAGGGATGATTCTTGCAAACACTACATCTGTGGGAATGAAACCGAAAATTAATGACACTCCCATTCCCAAGCATGCTTTGAAGCATTACTCCCTAGTATTTGATGCCATATACACACCAAAAGACACTAGACTCTTGAGGGAAGCAAAAGAGAGTGGAAAAATTATAGTTTATGGGACAGAGATGTTGATCCGCCAAGGCTTTGAACAATACAAGAACTTCACTGGTTTGCCTGCCCCTGAAGAATTGTTTAGGGAGCTTATGTCGAAACACGCATAA
Protein Sequence
  • >Vitvi14g00339_P001
    MGSLPFTVSDLQTSVSGVRSNPTLLCTPLMGTTVEQMLTEMRKAKEIGADIVEIRLDCLRNFSPAQDLQILIKQSPLPTLVTYRPIWEGGQYEGDENKRQDALRLAMELGASYIDVELEVAHEFNNSIYGKKPQNFKVIVSSHNFHNTPSTEAIGNLVARIQASGADIVKIATTALDITDVARVLQVTVHSQVPTIAIVMGERGLISRLLSPKFGGYLTYGALEAGAISAPGQPTAKDLLDLYNFRLIKPDTKVYGIIGKPVGHSKSPLLFNAAFKKVGLNAVYVHLLVDDVEKFFNTYSAPDFISGCSCTIPHKEVAIKCMDTIDPIARKIGAINNIVRKPDGKLTAFNTDYIGAIEAIEDGLRESNGSSPAVGSPLAGKLFVVLGAGGAGKSLAYGAKEKGARVVVANRTFERAKDLADKVGGQALTLAEIENFHPEEGMILANTTSVGMKPKINDTPIPKHALKHYSLVFDAIYTPKDTRLLREAKESGKIIVYGTEMLIRQGFEQYKNFTGLPAPEELFRELMSKHA