Gene Details:
- Gene ID: Vitvi08g02371
- Gene Symbol: VvSIP1
- Gene Name: small basic intrinsic protein 1
- Genome: Vitis vinifera PN40024.v4
- Species: Vitis vinifera
Functional Descriptions:
- The grape aquaporin VvSIP1 transports water across the ER membrane.
- VvSIP1 was expressed in leaves and berries from field-grown vines, and in leaves and stems from in vitro plantlets, but not in roots.
- When expressed in tobacco mesophyll cells and in Saccharomyces cerevisiae, fluorescent-tagged VvSIP1 was localized at the endoplasmic reticulum (ER). Stopped-flow spectroscopy showed that VvSIP1-enriched ER membrane vesicles from yeast exhibited higher water permeability and lower activation energy for water transport than control vesicles, indicating the involvement of protein-mediated water diffusion.
- To provide further insights into gene function, the expression of VvSIP1 in mature grapes was studied when vines were cultivated in different field conditions, but its transcript levels did not increase significantly in water-stressed plants and western-exposed berries. However, the expression of the aquaporin genes VvSIP1, VvPIP2|2, and VvTIP1|1 was up-regulated by heat in cultured cells.
Function-related keywords:
Literature:
- The grape aquaporin VvSIP1 transports water across the ER membrane. DOI: 10.1093/jxb/ert448 ; PMID: 24376256
Related News:
Gene Resources:
Orthologs:
Sequences:
cDNA Sequence
- >Vitvi08g02371_t001
GAATCCTGAAATAGGAAAGGGAATTCGTTGGCGAGGAGTGGCGAGATAAAATATATACACTATCGGGGCGCGGTTAGGTTAGGTAAGGTTGATCATCCATCCGGTGCAGGAAAGGCGGATATAGCCGTCTGTCTCTCTCCGCACGAGGAGAGAAAAAGGGGAGGGGAAAGGGGGAGCCGAGAAGGATTTTGCGTTATTGAGAGGGAGGATGGGTGTAATAAAGGCGGCGATTGGTGATGGGGTGTTGACTTTCATGTGGGTGTTCTGCGTTTCGACGGTGGGGATATTCACCTCTCTCATATCGTCGGCCTTGGGGGTTCAGGCGCTGCCGTTGGCTGCGATTTTCATCACCACTGTCCTTATCTTTGTTCTTGTGTTCGTGTTCAGCGCGATAGGGGATGTCTTGGGTGGGGCCAGCTTCAATCCCACTGCAACTGCTGCGCTCTATGCCGCCGGTGTCACACCCGACAGCCTCATCTCCATGGCTTTCCGTTTTCCTGCTCAGGCAGCTGGTGCTGTGGGTGGTGCCCTGGCAATCATGGAGGTGATGCCAACACAGTACAAACACATGCTTGGGGGGCCTTCTTTGAAAGTGGACTTGCATACTGGAGCCATTGCTGAGGGGATTTTGACCTTTACAATTAGCTTTCTCGTGCTTTTGGTTGTCCTCAAAGGTCCAAAGAGTCCAGTATTGAAGACATGGTTGCTTGCCATGGCGACTGTAACTCTGGTCGTCTCAGGCTCTGCTTACACTGGGCCTTCCATGAATCCCGCCAATGCATTTGGATGGGCATATATAAACAATTGGCATAACACATGGGACCAGTTGTACGTTTACTGGATCTGCCCCTTCATAGGGGCAATATTGGCTGCATGGGTCTTCCGATTCCTATTTCCAGCACCAACAAAGCAGAAGAAAGCCTGAGAAGGGGCATGAACTGAAATTCTATGTTAGTAGGTAAGTTAACTTAATAGGATTTTATTCTATTTTCTGGTTGTGGGCTGAAACTATCATCTGTCTAGTGAATAAACGTGGAAGAAGATGGAAGTAGAACAAATCTCTAGTGAATCATTGTGATGTAGATTTAAGTCGCATCTTGAATTTGGGCCTTTGAGAACAAATTTGGCTTGATTGCTTGGAATCCCGCATTTTCTTCCTTCAGGGTTTGATTTGGCCCAAACTATGCAAAGCTCTTTTTTTTTTTTTACCTTCGTTTTATTCAAGCTTTTTATTGGTGGACTCGATCTTATGGATCATGGTTGAGAGTATGTGATAGAACCGTTGATTAAATTATAAATCTGCCTCAGCCACTGTGATTTCGCATTATACGTGACATGATTCCATATTGAAAATGTATCATCAAGCATGGATTGTCATATATGCATATCCCTATCCTCCCAGGTTCCATTGCTTTATGCCCAACGTATTATTCCTTTCTATTTTATGTCGGATTTCATTGCTTTGTACATGTTTATATCCTAAAACTGAACGATCTAAATGACTCATAGCGATTGCATGGAATTGGTAGCATGGATTTTTAAAAATGGGATGTGGCCCCAGAAAAGATGGAAAATTGGGGTACCTCTTTGGATAATACCGAGTTCGGCCTAACTCATTAGGCAAATGTAAAACTAGTGCATAAGTGTTTGACTATAGATAACCAGTAGGTGCTGGATTAGGTGAACTCCACGTGGAAAACTACAGAATGTCGCAGTCAAGACTTAGCAGTTGGATTCGATTTTGAGCTCACACATTTTCTTGTTCATCATGGGTTTGACTAGTCTGTTATGCAAGTATGGTCTCTATCCTTTTAACTAGAACCGCCTGCTCGCATGCATATTCATATTCGCCCTGTCGTGCTTTCCCTTCTTGGGGTTTTCCCAAAGTTTTCAATGTTCTTGGGATCCTAGAACAGAGCTCTGAATATTTCAAAGCTGACAAGAGAGTTATCGGTGGTTTATCACGCGGA
CDS Sequence
- >Vitvi08g02371_t001
ATGGGTGTAATAAAGGCGGCGATTGGTGATGGGGTGTTGACTTTCATGTGGGTGTTCTGCGTTTCGACGGTGGGGATATTCACCTCTCTCATATCGTCGGCCTTGGGGGTTCAGGCGCTGCCGTTGGCTGCGATTTTCATCACCACTGTCCTTATCTTTGTTCTTGTGTTCGTGTTCAGCGCGATAGGGGATGTCTTGGGTGGGGCCAGCTTCAATCCCACTGCAACTGCTGCGCTCTATGCCGCCGGTGTCACACCCGACAGCCTCATCTCCATGGCTTTCCGTTTTCCTGCTCAGGCAGCTGGTGCTGTGGGTGGTGCCCTGGCAATCATGGAGGTGATGCCAACACAGTACAAACACATGCTTGGGGGGCCTTCTTTGAAAGTGGACTTGCATACTGGAGCCATTGCTGAGGGGATTTTGACCTTTACAATTAGCTTTCTCGTGCTTTTGGTTGTCCTCAAAGGTCCAAAGAGTCCAGTATTGAAGACATGGTTGCTTGCCATGGCGACTGTAACTCTGGTCGTCTCAGGCTCTGCTTACACTGGGCCTTCCATGAATCCCGCCAATGCATTTGGATGGGCATATATAAACAATTGGCATAACACATGGGACCAGTTGTACGTTTACTGGATCTGCCCCTTCATAGGGGCAATATTGGCTGCATGGGTCTTCCGATTCCTATTTCCAGCACCAACAAAGCAGAAGAAAGCCTGA
Protein Sequence
- >Vitvi08g02371_P001
MGVIKAAIGDGVLTFMWVFCVSTVGIFTSLISSALGVQALPLAAIFITTVLIFVLVFVFSAIGDVLGGASFNPTATAALYAAGVTPDSLISMAFRFPAQAAGAVGGALAIMEVMPTQYKHMLGGPSLKVDLHTGAIAEGILTFTISFLVLLVVLKGPKSPVLKTWLLAMATVTLVVSGSAYTGPSMNPANAFGWAYINNWHNTWDQLYVYWICPFIGAILAAWVFRFLFPAPTKQKKA