Gene Details:
- Gene ID: Vitvi08g01055
- Gene Symbol: VvMLO3
- Gene Name: mildew resistance Locus O3
- Genome: Vitis vinifera PN40024.v4
- Species: Vitis vinifera
Functional Descriptions:
- CRISPR/Cas9-mediated mutagenesis of VvMLO3 results in enhanced resistance to powdery mildew in grapevine.
- Suggesting that signals from mildew-invaded epidermal cells trigger the collapse of neighboring mesophyll cells as a result of the mutation of VvMLO3 in these heterozygous lines.
- Importantly, four VvMLO3-edited lines showed enhanced resistance to powdery mildew, which was associated with host cell death, cell wall apposition (CWA) and H2O2 accumulation.
- VvMLO3-edited grapevine plants show infection-triggered cell death, H2O2 accumulation and CWAs.
Function-related keywords:
- resistance , cell-death , cell-wall , powdery-mildew , mildew-resistance , mildew , powdery-mildew-resistance , h2o2
Literature:
- CRISPR/Cas9-mediated mutagenesis of VvMLO3 results in enhanced resistance to powdery mildew in grapevine (Vitis vinifera). DOI: 10.1038/s41438-020-0339-8 ; PMID: 32821399
Related News:
Gene Resources:
- NCBI ID: EU812234.1
- UniProt accessions: C6EWE6
Orthologs:
Sequences:
cDNA Sequence
- >Vitvi08g01055_t001
GGCCATATAGAAAGGATATGGCTAAGGGATCAAAGGATCGATCTTTGGAGCAAACACCGACTTGGGCGGTTGCAGTGGTCTGTTTTGTGCTGGTTTTGATATCAATTATCATCGAATACATCCTTCACTTAATTGGAAAGTGGCTAACAAAGAGAAACAAACGAGCTCTTTATGAAGCACTTGAAAAGATTAAGTCAGAACTTATGCTACTGGGGTTCATATCGCTGCTCCTAACGGTAGGACAAGGAACTATTGCGGGAATATGCATATCAGAGAAGATTGCAGCAACCTGGCACCCATGTGGGAAGAAACAAGAAATCAAGTATGTTTCTAATGAAGAAGATTATGGCAAGAGAAGGCTTCTTGAAATTTCAGATTCCGATGGAAGTAATCGACGTGTGTTAGCGGCCGCGGGAGATGACAAATGTGGAGAGGGTAAAGTCCCGTTTGTCTCCAATTATGGGATCCACCAACTTCACATATTCATCTTCGTTCTTGCTGTTTTCCACGTACTTTATTGTATAATCACATTGGCTTTGGGCAGAGCTAAGATGAGGAAGTGGAAGGCGTGGGAAATGGAAACAAGAACAGCCGAGTACCGGTTCGCAAACGATCCAGAGAGATTTAGGTTTGCAAGAGACACCTCATTTGGGAGAAGGCATTTGCACTCCTGGAGCACCTCCCCAGTTCTCCTTTGGATTGTGTGTTTCTTCAGACAATTTGTCAGATCAGTTCCCAAAGTTGATTACTTGACCTTGCGCCATGGGTTTATCATTGCACATTTGGCACCCGAGAGTCATACTAGATTTGATTTCCAGAAATACATCAAGAGATCACTCGAGGAGGATTTCAAAGTTGTAGTCGGTATCAGTCCAATAATCTGGTTCTGTGCTGTACTCTTCCTACTATTCAACACCCATGGTTGGCATTCTTATCTATGGTTACCCTTTATCCCACTAATTATCATCCTGATGGTGGGGACAAAACTACAAGTTATCATAACAAAGATGGGGCTGAGAATACAGGAGAGAGGAGAGGTGGTAAAAGGAACCCCAGTGGTGGAGCCTGGTGATGATCTTTTCTGGTTCAACCAGCCACGTCTCATTCTCTACCTGATTAACTTTGTTCTCTTTCAGAACGCATTCCAGGTTGCCTTCTTTGCATGGACCTGGTATGAGTTTGGCTTGAAATCTTGTTTCCACGAAAGGATAGAAGATGTGGTCATCCGCATATCAATGGGAGTCATAGTACAAATACTCTGCAGCTATGTGACTCTTCCTCTGTATGCCTTGGTTACACAGATGGGATCTACCATGAAGCCCACCATCTTCAATGACAGAGTGGCGAAAGCTCTGAGAAACTGGCACCACGCTGCAAGGAAGCACATAAAACAGAGCAAGCAATCAAGCGCTGTGACCCCTGTATCAAGTAGGGCAGGCACTCCCTTTTCAAGTAGGCCAGGCACCCCCTTACATGGCATGTCCCCTGTTCATCTACTCCGCCACCACCGCAGTGAGCTCGACAGTGTTCAAACATCTCCTAGAATGTCCAATTTTGACAATGAAGGTCCGGAGACAGACGAGTATCGCCACCGTGAGGATATATCATGGTCAGAACATCATAGAAATCCTGGTCCAGAAGAAGAGGGGAGGGACACAAATCATAGGATCTTGACCCGTACCATGCCAGCTCCTCAAGCTGACAATGCTCAGCACGAAATTGACATTCAGCCCATGGACTTTTCATTCGATAAAAGAGCAAGAACTTGA
CDS Sequence
- >Vitvi08g01055_t001
ATGGCTAAGGGATCAAAGGATCGATCTTTGGAGCAAACACCGACTTGGGCGGTTGCAGTGGTCTGTTTTGTGCTGGTTTTGATATCAATTATCATCGAATACATCCTTCACTTAATTGGAAAGTGGCTAACAAAGAGAAACAAACGAGCTCTTTATGAAGCACTTGAAAAGATTAAGTCAGAACTTATGCTACTGGGGTTCATATCGCTGCTCCTAACGGTAGGACAAGGAACTATTGCGGGAATATGCATATCAGAGAAGATTGCAGCAACCTGGCACCCATGTGGGAAGAAACAAGAAATCAAGTATGTTTCTAATGAAGAAGATTATGGCAAGAGAAGGCTTCTTGAAATTTCAGATTCCGATGGAAGTAATCGACGTGTGTTAGCGGCCGCGGGAGATGACAAATGTGGAGAGGGTAAAGTCCCGTTTGTCTCCAATTATGGGATCCACCAACTTCACATATTCATCTTCGTTCTTGCTGTTTTCCACGTACTTTATTGTATAATCACATTGGCTTTGGGCAGAGCTAAGATGAGGAAGTGGAAGGCGTGGGAAATGGAAACAAGAACAGCCGAGTACCGGTTCGCAAACGATCCAGAGAGATTTAGGTTTGCAAGAGACACCTCATTTGGGAGAAGGCATTTGCACTCCTGGAGCACCTCCCCAGTTCTCCTTTGGATTGTGTGTTTCTTCAGACAATTTGTCAGATCAGTTCCCAAAGTTGATTACTTGACCTTGCGCCATGGGTTTATCATTGCACATTTGGCACCCGAGAGTCATACTAGATTTGATTTCCAGAAATACATCAAGAGATCACTCGAGGAGGATTTCAAAGTTGTAGTCGGTATCAGTCCAATAATCTGGTTCTGTGCTGTACTCTTCCTACTATTCAACACCCATGGTTGGCATTCTTATCTATGGTTACCCTTTATCCCACTAATTATCATCCTGATGGTGGGGACAAAACTACAAGTTATCATAACAAAGATGGGGCTGAGAATACAGGAGAGAGGAGAGGTGGTAAAAGGAACCCCAGTGGTGGAGCCTGGTGATGATCTTTTCTGGTTCAACCAGCCACGTCTCATTCTCTACCTGATTAACTTTGTTCTCTTTCAGAACGCATTCCAGGTTGCCTTCTTTGCATGGACCTGGTATGAGTTTGGCTTGAAATCTTGTTTCCACGAAAGGATAGAAGATGTGGTCATCCGCATATCAATGGGAGTCATAGTACAAATACTCTGCAGCTATGTGACTCTTCCTCTGTATGCCTTGGTTACACAGATGGGATCTACCATGAAGCCCACCATCTTCAATGACAGAGTGGCGAAAGCTCTGAGAAACTGGCACCACGCTGCAAGGAAGCACATAAAACAGAGCAAGCAATCAAGCGCTGTGACCCCTGTATCAAGTAGGGCAGGCACTCCCTTTTCAAGTAGGCCAGGCACCCCCTTACATGGCATGTCCCCTGTTCATCTACTCCGCCACCACCGCAGTGAGCTCGACAGTGTTCAAACATCTCCTAGAATGTCCAATTTTGACAATGAAGGTCCGGAGACAGACGAGTATCGCCACCGTGAGGATATATCATGGTCAGAACATCATAGAAATCCTGGTCCAGAAGAAGAGGGGAGGGACACAAATCATAGGATCTTGACCCGTACCATGCCAGCTCCTCAAGCTGACAATGCTCAGCACGAAATTGACATTCAGCCCATGGACTTTTCATTCGATAAAAGAGCAAGAACTTGA
Protein Sequence
- >Vitvi08g01055_P001
MAKGSKDRSLEQTPTWAVAVVCFVLVLISIIIEYILHLIGKWLTKRNKRALYEALEKIKSELMLLGFISLLLTVGQGTIAGICISEKIAATWHPCGKKQEIKYVSNEEDYGKRRLLEISDSDGSNRRVLAAAGDDKCGEGKVPFVSNYGIHQLHIFIFVLAVFHVLYCIITLALGRAKMRKWKAWEMETRTAEYRFANDPERFRFARDTSFGRRHLHSWSTSPVLLWIVCFFRQFVRSVPKVDYLTLRHGFIIAHLAPESHTRFDFQKYIKRSLEEDFKVVVGISPIIWFCAVLFLLFNTHGWHSYLWLPFIPLIIILMVGTKLQVIITKMGLRIQERGEVVKGTPVVEPGDDLFWFNQPRLILYLINFVLFQNAFQVAFFAWTWYEFGLKSCFHERIEDVVIRISMGVIVQILCSYVTLPLYALVTQMGSTMKPTIFNDRVAKALRNWHHAARKHIKQSKQSSAVTPVSSRAGTPFSSRPGTPLHGMSPVHLLRHHRSELDSVQTSPRMSNFDNEGPETDEYRHREDISWSEHHRNPGPEEEGRDTNHRILTRTMPAPQADNAQHEIDIQPMDFSFDKRART