Gene Details:

Functional Descriptions:

  • The Grapevine Root-Specific Aquaporin **VvPIP2|4N **|**VvPIP2|4N **Controls Root Hydraulic Conductance and Leaf Gas Exchange under Well-Watered Conditions But Not under Water Stress.
  • Expression of **VvPIP2|4N **|**VvPIP2|4N **in Xenopus laevis oocytes increased their swelling rate 54-fold. Northern blot and quantitative reverse transcription-polymerase chain reaction analyses showed that **VvPIP2|4N **|**VvPIP2|4N **is the most expressed PIP2 gene in root.
  • We then constitutively overexpressed **VvPIP2|4N **|**VvPIP2|4N **in grape ‘Brachetto’, and in the resulting transgenic plants we analyzed (1) the expression of endogenous and transgenic **VvPIP2|4N **|**VvPIP2|4N **and of four other aquaporins, (2) whole-plant, root, and leaf ecophysiological parameters, and (3) leaf abscisic acid content. Expression of transgenic **VvPIP2|4N **|**VvPIP2|4N **inhibited neither the expression of the endogenous gene nor that of other PIP aquaporins in both root and leaf.
  • The leaf component of total plant hydraulic resistance was low and unaffected by overexpression of **VvPIP2|4N **|4N. Upon water stress, the overexpression of **VvPIP2|4N **|**VvPIP2|4N **induced a surge in leaf abscisic acid content and a decrease in stomatal conductance and leaf gas exchange.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Vitvi06g00281_t001
    CTCACATCAAAGCTTTCTCTCTCTTGTGTAAAAGTTTGCCGAGACAGCGGTGGTAATCAATGACGAAAGACGTTGAAACCTCGGGACAAGAGTTTTCGAACAAGGACTACCATGATCCACCTCCGGCGCCGCTGATCGACCCAGATGAATTAACACAATGGTCCTTATACAGAGCCATCATCGCCGAGTTTATCGCCACCCTGCTTTTCCTCTACATCACTGTCCTAACAGTGATCGGATACAAGAGCCAGTCCGACACCGACCCATGCGGGGGCGTGGGCATCTTGGGTATCGCCTGGGCCTTCGGCGGAATGATCTTCATCCTAGTCTACTGCACCGCCGGCATCTCCGGAGGACACATAAACCCGGCGGTGACCTTTGGGCTGTTCTTGGCGAGGAAGGTGTCGCTGGTGAGGGCTCTCATGTACATGATAGCTCAGTGCCTGGGTGCAGTCTGCGGTGTGGGCTTGGTGAAGGCGTTTCAGTCTTCGCTCTACAATAGGTACGGTGGGGGAGCAAACGAGCTGGCTGGTGGCTACAACAAGGGCGTGGGATTGGGTGCTGAGATCATCGGCACCTTTGTTCTTGTCTACACCGTCTTCTCTGCCACTGACCCCAAGAGGAACGCCCGAGACTCCCATGTTCCTGTTTTGGCACCACTGCCCATCGGATTTGCGGTGTTCATGGTTCACCTGGCAACAATTCCCATCACCGGCACCGGTATCAACCCGGCTAGAAGCTTCGGAGCTGCTGTCATCTATAACAACGACAAGGCCTGGGATGACCATTGGATGTTCTGGGTCGGACCATTCATTGGAGCTGCCATTGCTGCATTCTACCACCAGTATATTCTGAGAGCAGCGGCCATTAAAGCTCTAGGATCTTTCAGGAGCAATGCCTAGGTTTTGGACGCTAGCTGGTGATGGTGATGGTGATGGTTTCTGCAACAAGAATAACATCAATGGTGGAGGAGTATTAGTACATGCAAATCGGTCTCAATCTCTTTCCAGTGTTTTTTCTCTTCTATCTGCCCCCTCTCTCTCCCTCTCTCCCTCTCTCCCTCCCTTGGAACCATGGAATTGTAGATAAATTTGGCTTTTATCTGGCCATGTCTCTTTAAATATATTTCAAGTGCTGTATGTTGTCATCTTTTTTCTTTTCCCTCCTCTCTCTCTCTCTTTCTACTTGGTGTATTCATAATGATGCTTCCACATTATTCTAGTAAATGGCTATGCTCCTCAATCTCCATCACCCC
CDS Sequence
  • >Vitvi06g00281_t001
    ATGACGAAAGACGTTGAAACCTCGGGACAAGAGTTTTCGAACAAGGACTACCATGATCCACCTCCGGCGCCGCTGATCGACCCAGATGAATTAACACAATGGTCCTTATACAGAGCCATCATCGCCGAGTTTATCGCCACCCTGCTTTTCCTCTACATCACTGTCCTAACAGTGATCGGATACAAGAGCCAGTCCGACACCGACCCATGCGGGGGCGTGGGCATCTTGGGTATCGCCTGGGCCTTCGGCGGAATGATCTTCATCCTAGTCTACTGCACCGCCGGCATCTCCGGAGGACACATAAACCCGGCGGTGACCTTTGGGCTGTTCTTGGCGAGGAAGGTGTCGCTGGTGAGGGCTCTCATGTACATGATAGCTCAGTGCCTGGGTGCAGTCTGCGGTGTGGGCTTGGTGAAGGCGTTTCAGTCTTCGCTCTACAATAGGTACGGTGGGGGAGCAAACGAGCTGGCTGGTGGCTACAACAAGGGCGTGGGATTGGGTGCTGAGATCATCGGCACCTTTGTTCTTGTCTACACCGTCTTCTCTGCCACTGACCCCAAGAGGAACGCCCGAGACTCCCATGTTCCTGTTTTGGCACCACTGCCCATCGGATTTGCGGTGTTCATGGTTCACCTGGCAACAATTCCCATCACCGGCACCGGTATCAACCCGGCTAGAAGCTTCGGAGCTGCTGTCATCTATAACAACGACAAGGCCTGGGATGACCATTGGATGTTCTGGGTCGGACCATTCATTGGAGCTGCCATTGCTGCATTCTACCACCAGTATATTCTGAGAGCAGCGGCCATTAAAGCTCTAGGATCTTTCAGGAGCAATGCCTAG
Protein Sequence
  • >Vitvi06g00281_P001
    MTKDVETSGQEFSNKDYHDPPPAPLIDPDELTQWSLYRAIIAEFIATLLFLYITVLTVIGYKSQSDTDPCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLVRALMYMIAQCLGAVCGVGLVKAFQSSLYNRYGGGANELAGGYNKGVGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWMFWVGPFIGAAIAAFYHQYILRAAAIKALGSFRSNA