Gene Details:
- Gene ID: Vitvi06g00281
- Gene Symbol: VvPIP2
- Gene Name: aquaporin PIP2
- Genome: Vitis vinifera PN40024.v4
- Species: Vitis vinifera
Functional Descriptions:
- The Grapevine Root-Specific Aquaporin **VvPIP2|4N **|**VvPIP2|4N **Controls Root Hydraulic Conductance and Leaf Gas Exchange under Well-Watered Conditions But Not under Water Stress.
- Expression of **VvPIP2|4N **|**VvPIP2|4N **in Xenopus laevis oocytes increased their swelling rate 54-fold. Northern blot and quantitative reverse transcription-polymerase chain reaction analyses showed that **VvPIP2|4N **|**VvPIP2|4N **is the most expressed PIP2 gene in root.
- We then constitutively overexpressed **VvPIP2|4N **|**VvPIP2|4N **in grape ‘Brachetto’, and in the resulting transgenic plants we analyzed (1) the expression of endogenous and transgenic **VvPIP2|4N **|**VvPIP2|4N **and of four other aquaporins, (2) whole-plant, root, and leaf ecophysiological parameters, and (3) leaf abscisic acid content. Expression of transgenic **VvPIP2|4N **|**VvPIP2|4N **inhibited neither the expression of the endogenous gene nor that of other PIP aquaporins in both root and leaf.
- The leaf component of total plant hydraulic resistance was low and unaffected by overexpression of **VvPIP2|4N **|4N. Upon water stress, the overexpression of **VvPIP2|4N **|**VvPIP2|4N **induced a surge in leaf abscisic acid content and a decrease in stomatal conductance and leaf gas exchange.
Function-related keywords:
- leaf , root , resistance , stress , stomatal , abscisic-acid
Literature:
- The grapevine root-specific aquaporin VvPIP2;4N controls root hydraulic conductance and leaf gas exchange under well-watered conditions but not under water stress. DOI: 10.1104/pp.112.203455 ; PMID: 22923680
Related News:
Gene Resources:
- NCBI ID: DQ358107.1
- UniProt accessions: Q2HZF5
Orthologs:
Sequences:
cDNA Sequence
- >Vitvi06g00281_t001
CTCACATCAAAGCTTTCTCTCTCTTGTGTAAAAGTTTGCCGAGACAGCGGTGGTAATCAATGACGAAAGACGTTGAAACCTCGGGACAAGAGTTTTCGAACAAGGACTACCATGATCCACCTCCGGCGCCGCTGATCGACCCAGATGAATTAACACAATGGTCCTTATACAGAGCCATCATCGCCGAGTTTATCGCCACCCTGCTTTTCCTCTACATCACTGTCCTAACAGTGATCGGATACAAGAGCCAGTCCGACACCGACCCATGCGGGGGCGTGGGCATCTTGGGTATCGCCTGGGCCTTCGGCGGAATGATCTTCATCCTAGTCTACTGCACCGCCGGCATCTCCGGAGGACACATAAACCCGGCGGTGACCTTTGGGCTGTTCTTGGCGAGGAAGGTGTCGCTGGTGAGGGCTCTCATGTACATGATAGCTCAGTGCCTGGGTGCAGTCTGCGGTGTGGGCTTGGTGAAGGCGTTTCAGTCTTCGCTCTACAATAGGTACGGTGGGGGAGCAAACGAGCTGGCTGGTGGCTACAACAAGGGCGTGGGATTGGGTGCTGAGATCATCGGCACCTTTGTTCTTGTCTACACCGTCTTCTCTGCCACTGACCCCAAGAGGAACGCCCGAGACTCCCATGTTCCTGTTTTGGCACCACTGCCCATCGGATTTGCGGTGTTCATGGTTCACCTGGCAACAATTCCCATCACCGGCACCGGTATCAACCCGGCTAGAAGCTTCGGAGCTGCTGTCATCTATAACAACGACAAGGCCTGGGATGACCATTGGATGTTCTGGGTCGGACCATTCATTGGAGCTGCCATTGCTGCATTCTACCACCAGTATATTCTGAGAGCAGCGGCCATTAAAGCTCTAGGATCTTTCAGGAGCAATGCCTAGGTTTTGGACGCTAGCTGGTGATGGTGATGGTGATGGTTTCTGCAACAAGAATAACATCAATGGTGGAGGAGTATTAGTACATGCAAATCGGTCTCAATCTCTTTCCAGTGTTTTTTCTCTTCTATCTGCCCCCTCTCTCTCCCTCTCTCCCTCTCTCCCTCCCTTGGAACCATGGAATTGTAGATAAATTTGGCTTTTATCTGGCCATGTCTCTTTAAATATATTTCAAGTGCTGTATGTTGTCATCTTTTTTCTTTTCCCTCCTCTCTCTCTCTCTTTCTACTTGGTGTATTCATAATGATGCTTCCACATTATTCTAGTAAATGGCTATGCTCCTCAATCTCCATCACCCC
CDS Sequence
- >Vitvi06g00281_t001
ATGACGAAAGACGTTGAAACCTCGGGACAAGAGTTTTCGAACAAGGACTACCATGATCCACCTCCGGCGCCGCTGATCGACCCAGATGAATTAACACAATGGTCCTTATACAGAGCCATCATCGCCGAGTTTATCGCCACCCTGCTTTTCCTCTACATCACTGTCCTAACAGTGATCGGATACAAGAGCCAGTCCGACACCGACCCATGCGGGGGCGTGGGCATCTTGGGTATCGCCTGGGCCTTCGGCGGAATGATCTTCATCCTAGTCTACTGCACCGCCGGCATCTCCGGAGGACACATAAACCCGGCGGTGACCTTTGGGCTGTTCTTGGCGAGGAAGGTGTCGCTGGTGAGGGCTCTCATGTACATGATAGCTCAGTGCCTGGGTGCAGTCTGCGGTGTGGGCTTGGTGAAGGCGTTTCAGTCTTCGCTCTACAATAGGTACGGTGGGGGAGCAAACGAGCTGGCTGGTGGCTACAACAAGGGCGTGGGATTGGGTGCTGAGATCATCGGCACCTTTGTTCTTGTCTACACCGTCTTCTCTGCCACTGACCCCAAGAGGAACGCCCGAGACTCCCATGTTCCTGTTTTGGCACCACTGCCCATCGGATTTGCGGTGTTCATGGTTCACCTGGCAACAATTCCCATCACCGGCACCGGTATCAACCCGGCTAGAAGCTTCGGAGCTGCTGTCATCTATAACAACGACAAGGCCTGGGATGACCATTGGATGTTCTGGGTCGGACCATTCATTGGAGCTGCCATTGCTGCATTCTACCACCAGTATATTCTGAGAGCAGCGGCCATTAAAGCTCTAGGATCTTTCAGGAGCAATGCCTAG
Protein Sequence
- >Vitvi06g00281_P001
MTKDVETSGQEFSNKDYHDPPPAPLIDPDELTQWSLYRAIIAEFIATLLFLYITVLTVIGYKSQSDTDPCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLVRALMYMIAQCLGAVCGVGLVKAFQSSLYNRYGGGANELAGGYNKGVGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNDKAWDDHWMFWVGPFIGAAIAAFYHQYILRAAAIKALGSFRSNA