Gene Details:

• Gene ID: Vitvi06g00059
• Gene Symbol: VvMYB5b
• Gene Name: Transcription repressor MYB5
• Genome: Vitis vinifera PN40024.v4
• Species: Vitis vinifera

Functional Descriptions:

• Transgenic grapevine plants with altered expression of VvMYB5a, VvMYB5b, and VvWRKY26 show phenotypic aberrations which strongly support a role for the MBWW complex in controlling different aspects of grape plant development and the accumulation of flavonoids.
• VvMYB5a and VvMYB5b are able to replace the function of PhPH4 in the control of vacuolar hyper-acidification.
• Transcriptomic analyses define roles for VvMYB5a, VvMYB5b, and VvWRKY26 in phenylpropanoid metabolism, vacuolar acidification, cell type identity, and membrane transport.
• These results indicated that VvMYB5a and VvMYB5b are functionally distinct from the anthocyanin synthesis regulator VvMYBA1, and that both proteins are functionally interchangeable with the petunia homologue PH4.
• A single amino acid change within the R2 domain of the VvMYB5b transcription factor modulates affinity for protein partners and target promoters selectivity.
• In this work, we describe the effects of a single amino acid substitution (R69L) located in the R2 domain of VvMYB5b and predicted to affect the formation of a salt bridge within the protein.
• Flowers from 35S::VvMYB5bL transgenic plants showed a distinct phenotype in comparison with 35S::VvMYB5bR and the control plants. Finally, significant differences in transcript abundance of flavonoid metabolism genes were observed along with variations in pigments accumulation.
• Our findings indicate that VvMYB5bL is still able to bind DNA but the structural consequences linked to the mutation affect the capacity of the protein to activate the transcription of some flavonoid genes by modifying the interaction with its co-partner(s).

Function-related keywords:

• development ,  plant-development ,  anthocyanin , &nbsptranscription-factor ,  salt ,  flavonoid ,  flavonoid-metabolism ,  protein

Literature:

• The MYB5-driven MBW complex recruits a WRKY factor to enhance the expression of targets involved in vacuolar hyper-acidification and trafficking in grapevine.   DOI:  10.1111/tpj.14419 ;  PMID:  31125454
• A single amino acid change within the R2 domain of the VvMYB5b transcription factor modulates affinity for protein partners and target promoters selectivity.   DOI:  10.1186/1471-2229-11-117 ;  PMID:  21861899

Related News:

Gene Resources:

• NCBI ID: 100232973   AY899404.1
• UniProt accessions: F6GUM7   Q58QD0

Orthologs:

Sequences: