Gene Details:

Functional Descriptions:

  • Bread wheat TaSPO11-1 exhibits evolutionarily conserved function in meiotic recombination across distant plant species.
  • Although the wheat and Arabidopsis SPO11-1 proteins share only 55% identity and the partner proteins also differ, the TaSPO11-1-5D cDNA significantly restored the fertility of the Arabidopsis spo11-1 mutant, indicating a robust functional conservation of the SPO11-1 protein activity across distant plants.
  • This excludes the possibility that TaSPO11-1 induces DSBs independently of SPO11-2 (non-plant organisms have a single SPO11 protein), and confirms the need for the MTOPVIB to form DSBs.
  • We extend these comparisons in demonstrating the functionality and evolutionary conservation of wheat TaSPO11-1 through heterologous complementation of the corresponding Arabidopsis and rice mutant lines, i.e. across distant plant species.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >TraesCS5B02G396300.1
    ATGGCGGGGAGTGGGAAGAGGAGGCGCGCGACGGTGCTCGACGACGACGAGCGGCGGGGCCGGCGGAGGCTGGAGGAGGCGGCCCTGCTCCTCCACAAGATCAAAGGGCTGGTGGGCTGGGTGGTCGCGGAGATCAGCGCCGGCCGATCCCCTTCCCTGGCGCTCCACCGCTACCAGAACTACTGCGCCTGCGCCTCCGCCGCCGCCGCGGCGTCCCCGTCCACATGCGCCTGCAGCTACGACGCCCCCGTCGGCACGGACGTCCTCTCCCTCCTCCGCAAGGAATTCCACGCCTCCCGCCTCAATGTGCTTCTCAGGGTCCTGCTCGTGGTGCAGCAGCTCCTGCAGGAGAATAAGCACTGCTCCAAGAGGGACATCTACTACATGTACCCCTCCATGTTCGTAGAACAAGCGATTGTTGATCGTGCGATCAACGATATCTGCATACTCTTCAAGTGCAGCCGGCATAATCTCAACGTGGTTCCTGTGGCAAAAGGTTTGGTGATGGGCTGGATAAGATTTGTGGAGGGTGAAAAGAAAGTGTACTGCATAACAAACGTCAATACTGCTTTCCCCATTCCAGTTAGCATTGAAGCAATCAAAGATGTTGTTAGTGTTGCTCACTACATCCTTGTTGTTGAGAAGGAGGCAGTATTCCAGCGTTTGGCCAATGACAAGTTCTGCGAAAAGAATCGCTGCATTGTTATTACAGGAAGAGGCTACCCAGATATTCCAACAAGAAGATTCTTGCGCTACCTTGTCGAACAGCTGCACTTGCCTGCTTATTGCTTAGTGGATTCAGATCCGTATGGTTTTGATATTCTGGCCACCTATAAATTTGGTTCCATGCAATTGGCATATGATGCAAATATTCTGCGTGTGCCTGAGATACGATGGCTTGGGGTCTTCACATCTGACTTTGAGGAGTATTGTCTTCCAGACTGCTGTCAACTTCAATTGTCATCTGAAGATAGGAGGAAGCTTGAAGGAATTCTCACCAGATGTTACTTGCACAAGGAAGCCCCAGAATGGAGGTTGAAGTTGGAAGCCATGCTGGAAATGGGTGTCAAGTTTGAGATTGAAGCATTGTCAGCAAGTTCCATTTCGTTTCTGTCGCAGGAGTACATCCCCCAACAGATCAGACTAGGGAGGTACATATAGGATTGCATAGACCTGTATCTATGCAGAATTGTGCTAGGCTAAGTGTCTCCTTTTGTTTTGGTCACACCACTTGTAGTTGCTTGTCTGATCAAACAATCACCTTGGTATTCACAACAAGAATTTGACTGCAATTGGTGTATACCGTCAGTTACTGCATTGCTACTCATAATGCTTCTTGTTGTGCATAGATTAGTTTGGGTTTATTATGTTAATGTGCAAGGCAACCTGTTAGAAATGAATGCCTGAATAGGGAACTCTGGATGTCCTCTTAGTTCTACTAGGTCACCATCCCATGGAAGCAAGTTCCTATTTACCTGAGATCTTAGCCTCCACTTGCCGAGCATCATTAGATAGATTGTTGCTTGCCTTGCAATTCTGGAGCCAGAAACAACAATCCAAGAAAAAAAAATGCATCTACACAGGCTACCATAGTGGAAGCTGGAAATATTTCATGGCTGGAAAATAGTTTAACAAACTTCATGTACACAGGTACGTAAGCTTAGCGAAGCATCACCAAATACTTCTTTCTTACACATGTCCAGGCGCATATGAGCATATCCATTGTGTTAAATACATGTAGGAGCTCAACAAGGTCGTATCTTTTGACACTTTACTAGATTACAGAATTAATTAACAGTGTTAATCGACCATTCAACTAAAGTATTTAAGCTGTAAGATTAAGTACAGGCCCATATGTTCGTCACTGACCGGATTGTGCCTGAGTAAGGTCATATGTGGAGAGTGTGCCGGACTGGTCAACGTCGAGTTTGTCAAACTCCTCTAGAAAAGAAGATATGTCCTCTTGGCTGATCTTCCCCAGTTCTTTGAGCTTGTACACGACAAATTCAGCAGCACTGCACATATCAAAGTTCACAAATCAACACTCATTACCTACAAAATCTTAGGATATGATGGAGGGAAGAATACTGAAAACGCATTATGTGCTTACCCCACTTTCCGATCATTATCCAGATCAGCTGCTTCAAGATCCATGGTTGTTACTCTCCGTGTGAGAACCCATTTGGCCAGCATCTTTTGCCGTCGCTCGGTGTAGATCTCGGCGAGGTACATGAAGAAGAGCGCCAGAATTATGGTGCTTGTAATTATCCAAAACACCGCGAAAGTGCGCCCCAGTTGGGACGAGAAGCTTTTATCCCCATAACCCAAGGTAGTGATTGTGGCACAGACACAATAAAAGGAGTCAACAAGGCTCAGCTTCTCAACCTTCCAGAGAAAAACAGTCCCCACAACAATGGCCGTCACAAGAAGCAGAGCGGCCGTGTAGAACTTGTACTTTGTCTTGTTTGTCTCAATTTGTCTGAGCATTCTGGCCTCGCCACACTTCATATTCATGTGCAGTGCCTTGAAGAACAACACCTCCTGCTTCTCGACGAGATAGTCTGCCGACTTGCTCACGAAGAGAGCAATAATCGCCATGCCGGTGAAGACGAAAACACAAGCGAGCAGCTTTGTCGTGTCGCTGTTAGGAACAAGGTCTCCATAGCCCACCGATGTCATCGTGACAATGCAGAAGTACAGTGCGTCGAGCACTCTGTTGGTTCTCTTGCCAGATAACTGATCCATGACAAGGTAAAAGACTACGACGCCAACTAGCAGGTAGAGAAATAGGAGGAACCCTACTAGTCTGAAGCTGGGCCGTACCACGCTGAACAACTCCTTGGCTGGAAGCGACGAGCCATTTTCTTGTGGTTTTTCATCGGTCTCTGATCTGGGAGCCGACCTGCATCTTCGGAACCGCTTGGCTCCTTGCGACGGCTTTCTTTGGAGCACATTGGCATCGGGGTTCTCAGGTAGCAATGCTCGCTGAATGCTGTTGTCAGACATTGGCTTATGCTGGATTTGTTGTGAATTGCCGTCGCAATTGAAGCCCTATGATTCAGAGAATGGCACACGTTAGAGTATGGTTTTACAGATATGCGAGGAAGAAGTTTAGAAGCTCATTAAATATCAGGTAAAAAATAAGGCAAGACCGTGGATATGCACTGAATTGCATGAACCACCGAGAAACTCTATTCCTCTCAGCTTGTGTGAATTATACGTGTCCTAGCTGACACAAGAATTTCCTATCACCATCAGTTTAAGCATTAAATTATTGGACTGCAGGTCAAAGAGAGGCAGGCAAAATAAGTTTGGTCAAAAATAAGGGCCAATTCCTGGCTGTTATCTTGAATTCCTGATCTTTTTGACTTTCTGGGCCTCTAGGCACAGGAATTTTCCTCTTATCTTATCTAGGAGTACAGTATAGAAAACAAGTTACCTACTCTGTCGCTTTCTTTCTGGACAAAGTCGCACTGCTACTGGTTCAGATCGGATCTCTACTGCTAGCTTAATCTGCAACCCAAGGGATGAAACTGAGAGCCAAATGCTGGAAACCAACCTTGGGTTGAGATGGATTGTAAAAGAGGAGAGTAGGACGAGCAGGGGAAGCATTCCACTCTGCTACTAGCATACAGCAGGGCCATCTGCTTCTACTTGTGATGCAGCTACAGAATTCGGAAGGCAATTCTGGCAGCTCCGGCCAATCGCACGAAGCAAGCAGAGAACGGAGCACGGTTGAGAAGACGCAGAGATCATCCCTTACCTCCCTCCGGCTCCGGCCGACGGAAGCTGGGCGGAGCTTTTCTGGGTTTGAGGGCGCCTTCTTCCTTTTCTCCTTCCCTCGAGCTCTCCTGCTCTGCTCAGCT
CDS Sequence
  • >TraesCS5B02G396300.1
    ATGGCGGGGAGTGGGAAGAGGAGGCGCGCGACGGTGCTCGACGACGACGAGCGGCGGGGCCGGCGGAGGCTGGAGGAGGCGGCCCTGCTCCTCCACAAGATCAAAGGGCTGGTGGGCTGGGTGGTCGCGGAGATCAGCGCCGGCCGATCCCCTTCCCTGGCGCTCCACCGCTACCAGAACTACTGCGCCTGCGCCTCCGCCGCCGCCGCGGCGTCCCCGTCCACATGCGCCTGCAGCTACGACGCCCCCGTCGGCACGGACGTCCTCTCCCTCCTCCGCAAGGAATTCCACGCCTCCCGCCTCAATGTGCTTCTCAGGGTCCTGCTCGTGGTGCAGCAGCTCCTGCAGGAGAATAAGCACTGCTCCAAGAGGGACATCTACTACATGTACCCCTCCATGTTCGTAGAACAAGCGATTGTTGATCGTGCGATCAACGATATCTGCATACTCTTCAAGTGCAGCCGGCATAATCTCAACGTGGTTCCTGTGGCAAAAGGTTTGGTGATGGGCTGGATAAGATTTGTGGAGGGTGAAAAGAAAGTGTACTGCATAACAAACGTCAATACTGCTTTCCCCATTCCAGTTAGCATTGAAGCAATCAAAGATGTTGTTAGTGTTGCTCACTACATCCTTGTTGTTGAGAAGGAGGCAGTATTCCAGCGTTTGGCCAATGACAAGTTCTGCGAAAAGAATCGCTGCATTGTTATTACAGGAAGAGGCTACCCAGATATTCCAACAAGAAGATTCTTGCGCTACCTTGTCGAACAGCTGCACTTGCCTGCTTATTGCTTAGTGGATTCAGATCCGTATGGTTTTGATATTCTGGCCACCTATAAATTTGGTTCCATGCAATTGGCATATGATGCAAATATTCTGCGTGTGCCTGAGATACGATGGCTTGGGGTCTTCACATCTGACTTTGAGGAGTATTGTCTTCCAGACTGCTGTCAACTTCAATTGTCATCTGAAGATAGGAGGAAGCTTGAAGGAATTCTCACCAGATGTTACTTGCACAAGGAAGCCCCAGAATGGAGGTTGAAGTTGGAAGCCATGCTGGAAATGGGTGTCAAGTTTGAGATTGAAGCATTGTCAGCAAGTTCCATTTCGTTTCTGTCGCAGGAGTACATCCCCCAACAGATCAGACTAGGGAGGTACATATAG
Protein Sequence
  • >TraesCS5B02G396300.1
    MAGSGKRRRATVLDDDERRGRRRLEEAALLLHKIKGLVGWVVAEISAGRSPSLALHRYQNYCACASAAAAASPSTCACSYDAPVGTDVLSLLRKEFHASRLNVLLRVLLVVQQLLQENKHCSKRDIYYMYPSMFVEQAIVDRAINDICILFKCSRHNLNVVPVAKGLVMGWIRFVEGEKKVYCITNVNTAFPIPVSIEAIKDVVSVAHYILVVEKEAVFQRLANDKFCEKNRCIVITGRGYPDIPTRRFLRYLVEQLHLPAYCLVDSDPYGFDILATYKFGSMQLAYDANILRVPEIRWLGVFTSDFEEYCLPDCCQLQLSSEDRRKLEGILTRCYLHKEAPEWRLKLEAMLEMGVKFEIEALSASSISFLSQEYIPQQIRLGRYI