Gene Details:

Functional Descriptions:

  • TaWaxy and TaMTL, were edited with high efficiency by the optimized SpCas9 system, with the highest efficiency (80.5%) being achieved when using TaU3 and two sgRNAs to target TaWaxy.
  • A haploid induction rate of 18.9% was found in the TaMTL-edited T1 plants using the CRISPR/SpCas9 system. Mutants with reverse insertion of the deleted sequences of TaMTL and TaWaxy between the two sgRNAs were identified in the edited T0 plants.
  • In addition, wheat grains lacking embryos or endosperms were observed in the TaMTL-edited T1 generation.
  • We then applied the optimized system to edit TaMTL and TaWaxy to develop the induction of haploids and to improve starch quality.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Orthologs:

Sequences:

cDNA Sequence
CDS Sequence
Protein Sequence