Gene Details:

Functional Descriptions:

  • TaTHI2 interacts with Ca2+-dependent protein kinase TaCPK5 to suppress virus infection by regulating ROS accumulation.
  • TaTHI2 also interacted with the Ca2+-dependent protein kinase 5 (TaCPK5), which is localized in the plasma membrane, and promoted reactive oxygen species (ROS) production by repressing TaCPK5-mediated activity of the catalase protein TaCAT1.
  • Our results showed that TaTHI2 reduced the pIMAGO signal of TaCPK5 in a dose-dependent manner, indicating that TaCPK5 had autophosphorylation activity and that the autophosphorylation activity of TaCPK5 was repressed by TaTHI2.
  • We then investigated the role of TaTHI2 in plant immunity and found that it interacts with TaCPK5 to repress its kinase activity.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >TraesCS2D02G229100.1
    CTTCTTCTTCTTCTTTTCCCCTTCTGCTGTTCCTGTTTGAGTTCTGCTTCTAGATCCAGTTTCGGCTTTCTTTTCTTTTTATTCGAGTCTCTTGAATGAATTATTACTTTGTGGATTCAACGATGAGACGTGGATGAATTCAGGTTTCGATTGGTTTCGTCTGCTCGGCTGACTGGGTTGTAAATTTGGAACTGAAAGGAAGAATTTGAGCACAGATGGGCCAGTGTTGCAGCAGAGCTACGTCCCCGGATTCTGTCCAAGGAGGAGCCAATGGCTATGGCTATTCTCACCAGCCAAAACAAGCACAAGCACCTCCTAGTTACAACAATGCTCAGCCACCACCACAAGCTGAGGTGAGGTACACACCGCCAGCGATGAACCCTCCTGTAGTCCCACCTGTGGTTGCCCCCTCAAAGCCCATGCCGGACACGATTCTCGGCAAGCAGTACGAGGATGTGCGCTCTGTCTACTCCCTTGGCAAGGAACTTGGTAGGGGGCAGTTCGGGGTGACGTACCTTTGCACTGAGATTAGCACTGGCAGGCAGTATGCTTGCAAGTCCATATCCAAGCGCAAGCTCGTGAGTAAGGCTGACAAGGAGGATATCCGCAGGGAGATCCAGATCATGCAGCACCTATCTGGGCAGCCAAACATAGTGGAGTTCTGTGGAGCATATGAGGACAAGGGCAGCGTGCATGTCGTGATGGAGCTCTGTGCAGGCGGGGAGCTGTTTGATCGGATTATTGCCAAGGGGCACTACTCAGAGCGTGCAGCTGCTACAATCTGCAGAGGAGTTGTGAATGTTGTCAATGTTTGCCATTTCATGGGAGTGATGCACCGCGATCTGAAGCCGGAGAACTTCTTGCTTGCGACCAAGGATGAGAATGCAGTGCTCAAGGCCACTGATTTCGGGCTTTCGGTCTTCATTGAAGAAGGAAAAATGTATAGGGACATCGTTGGAAGTGCTTATTATGTTGCTCCTGAAGTCCTTAGGAGAAACTATGGTAAAGAGATAGATGTTTGGAGTGCAGGTGTTATTCTGTACATTCTTCTCAGCGGTGTTCCTCCATTCTGGGCTGAAACTGAGAAGGGAATATTTGATGCTATTCTTCAAGGGGAGATTGACTTTGAAAGTCAGCCATGGCCTTCAATTTCTGAGAGCGCTAAAGACCTTGTGAGGAAGATGTTGGCACAGGATCCAAAGAAAAGAATTAGTTCAGCCCAAGTTCTTCAACATCCATGGCTCAGGGAAGGAGAAGCATCAGATAAACCTATTGACAGTGCTGTTCTTTCTAGGATGAAGCAATTCAGAGCAATGAATAAACTGAAAAAGATGGCTCTAAAGGTTATAGCTTCAAATCTTAACGAGGAAGAGATCAAGGGCCTGAAGCAAATGTTTATGAACATGGACACAGACAACAGTGGGACAATCACATATGAGGAACTCAAAGCAGGACTAGCCAAACTTGGATCGAAGCTCTCAGAAGCTGAAGTAAAGCAGTTGATGGATGCGGCTGATGTGGATGGCAATGGATCAATTGACTACGTTGAGTTCATAACAGCAACCATGCATAGACACAAGCTCGAAAGAGACGAGCATTTGTTCAAGGCGTTCCAGTACTTTGACAAAGACAACAGTGGCTTCATCACAAGAGATGAACTGGAGACTGCTTTGATTGAGCATGAAATGGGCGATGCGGATACCATAAAGGACATCATATCAGAAGTCGACACAGATAATGATGGGAGGATTAACTACGAGGAATTCTGCGCTATGATGAGAGGAGGGATGCAGCAGCCAATAAGGCTCAAGTAGTCTTTCCTTGTAGAAATGGTGTACCATTAGCAACTGTTATTGGTTCCTTTTTTTTCGCTGCTGCACGGATGAGAAGTTCTCTTTGGCGTTGTTGAGATGATTGTGTATATCTTGTGGCCTTTTGCTCGTGCATGTTGTTCCTCTAGTGTTTTGGATCTACTTTCTTCTGTAAGTTGGTTTTTGCAAGTACATCTAATAAAAGAGTATTTTTGCACGTCAAATGTGCATATT
CDS Sequence
  • >TraesCS2D02G229100.1
    ATGGGCCAGTGTTGCAGCAGAGCTACGTCCCCGGATTCTGTCCAAGGAGGAGCCAATGGCTATGGCTATTCTCACCAGCCAAAACAAGCACAAGCACCTCCTAGTTACAACAATGCTCAGCCACCACCACAAGCTGAGGTGAGGTACACACCGCCAGCGATGAACCCTCCTGTAGTCCCACCTGTGGTTGCCCCCTCAAAGCCCATGCCGGACACGATTCTCGGCAAGCAGTACGAGGATGTGCGCTCTGTCTACTCCCTTGGCAAGGAACTTGGTAGGGGGCAGTTCGGGGTGACGTACCTTTGCACTGAGATTAGCACTGGCAGGCAGTATGCTTGCAAGTCCATATCCAAGCGCAAGCTCGTGAGTAAGGCTGACAAGGAGGATATCCGCAGGGAGATCCAGATCATGCAGCACCTATCTGGGCAGCCAAACATAGTGGAGTTCTGTGGAGCATATGAGGACAAGGGCAGCGTGCATGTCGTGATGGAGCTCTGTGCAGGCGGGGAGCTGTTTGATCGGATTATTGCCAAGGGGCACTACTCAGAGCGTGCAGCTGCTACAATCTGCAGAGGAGTTGTGAATGTTGTCAATGTTTGCCATTTCATGGGAGTGATGCACCGCGATCTGAAGCCGGAGAACTTCTTGCTTGCGACCAAGGATGAGAATGCAGTGCTCAAGGCCACTGATTTCGGGCTTTCGGTCTTCATTGAAGAAGGAAAAATGTATAGGGACATCGTTGGAAGTGCTTATTATGTTGCTCCTGAAGTCCTTAGGAGAAACTATGGTAAAGAGATAGATGTTTGGAGTGCAGGTGTTATTCTGTACATTCTTCTCAGCGGTGTTCCTCCATTCTGGGCTGAAACTGAGAAGGGAATATTTGATGCTATTCTTCAAGGGGAGATTGACTTTGAAAGTCAGCCATGGCCTTCAATTTCTGAGAGCGCTAAAGACCTTGTGAGGAAGATGTTGGCACAGGATCCAAAGAAAAGAATTAGTTCAGCCCAAGTTCTTCAACATCCATGGCTCAGGGAAGGAGAAGCATCAGATAAACCTATTGACAGTGCTGTTCTTTCTAGGATGAAGCAATTCAGAGCAATGAATAAACTGAAAAAGATGGCTCTAAAGGTTATAGCTTCAAATCTTAACGAGGAAGAGATCAAGGGCCTGAAGCAAATGTTTATGAACATGGACACAGACAACAGTGGGACAATCACATATGAGGAACTCAAAGCAGGACTAGCCAAACTTGGATCGAAGCTCTCAGAAGCTGAAGTAAAGCAGTTGATGGATGCGGCTGATGTGGATGGCAATGGATCAATTGACTACGTTGAGTTCATAACAGCAACCATGCATAGACACAAGCTCGAAAGAGACGAGCATTTGTTCAAGGCGTTCCAGTACTTTGACAAAGACAACAGTGGCTTCATCACAAGAGATGAACTGGAGACTGCTTTGATTGAGCATGAAATGGGCGATGCGGATACCATAAAGGACATCATATCAGAAGTCGACACAGATAATGATGGGAGGATTAACTACGAGGAATTCTGCGCTATGATGAGAGGAGGGATGCAGCAGCCAATAAGGCTCAAGTAG
Protein Sequence
  • >TraesCS2D02G229100.1
    MGQCCSRATSPDSVQGGANGYGYSHQPKQAQAPPSYNNAQPPPQAEVRYTPPAMNPPVVPPVVAPSKPMPDTILGKQYEDVRSVYSLGKELGRGQFGVTYLCTEISTGRQYACKSISKRKLVSKADKEDIRREIQIMQHLSGQPNIVEFCGAYEDKGSVHVVMELCAGGELFDRIIAKGHYSERAAATICRGVVNVVNVCHFMGVMHRDLKPENFLLATKDENAVLKATDFGLSVFIEEGKMYRDIVGSAYYVAPEVLRRNYGKEIDVWSAGVILYILLSGVPPFWAETEKGIFDAILQGEIDFESQPWPSISESAKDLVRKMLAQDPKKRISSAQVLQHPWLREGEASDKPIDSAVLSRMKQFRAMNKLKKMALKVIASNLNEEEIKGLKQMFMNMDTDNSGTITYEELKAGLAKLGSKLSEAEVKQLMDAADVDGNGSIDYVEFITATMHRHKLERDEHLFKAFQYFDKDNSGFITRDELETALIEHEMGDADTIKDIISEVDTDNDGRINYEEFCAMMRGGMQQPIRLK