Gene Details:

Functional Descriptions:

  • The two RNA motifs we chose occur in the cis-element of TaXa21-H and TaWRKY53, and we validated that Pst_A23 overexpression results in decreased functional transcripts of TaXa21-H and TaWRKY53 while silencing of TaXa21-H and TaWRKY53 impairs wheat resistance to Pst.
  • Silencing of TaXa21-H and TaWRKY53 compromises plant resistance to Pst.
  • These data indicate that Pst_A23 binding to the cis-element likely results in the decreased AS of TaXa21-H and TaWRKY53 and the R1R2A mutation affects its regulatory role on AS of TaXa21-H and TaWRKY53.
  • It is reasonable that the repressed functional transcripts of TaXa21-H and TaWRKY53 by Pst_A23 might contribute to the compromised wheat resistance to stripe rust.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >TraesCS6D02G206100.1
    GGCATCGAAATCTAAGTACAAACCACCGGCAAAGCCAAGCTGCCAAAGCTGCCGTTTTGTGCGCTCCTGTCGAGCAGAGCTGCAGAGGTACAACGGGGTTCATGTCTGACCAATCCGTGAAACTCAACATGCTTCTTCTGCTGGCGTTTCTGCTGCTGTCTTATGGAGCTGGCAATGCCCGTTGCTCAACTGTTCATGCGAACATCACAGACATTCTCTCCTTGCTCCGATTCAAAAGGTCCACCCACGATCCAACAGGTTCCTTGAGGAACTGGAACCGAAGCATCCATTACTGCAAGTGGAATGGTGTCTCCTGCAGCTTACTGAATCCAGGGCGGGTGGCGGCTTTGGATCTCCCTGGCCAAAACTTGTCAGGTCAAGTCAACCCTTCTCTTGGGAACATAACGTTCCTTAAGCGCCTGAATTTGTCCTCCAATGGCTTCTCCGGCCAGTTACCTCCTCTGAGTCAGCTCCATGAGCTGACGCTTCTTGACATGAGCTCTAACTTATTCCAAGGGATAATCCCCGATTCACTCACACAATTTTCGAACCTACAGCTCCTGAATTTGTCCTACAATGGCTTCTCCGGCCAGTTACCTCCTCTGAACCAGCTTCCCGAGCTGGTGGTTCTTGACTTGAAATCCAATTTATTCCAAGGGATAATCCCCGACTCACTCACAAACTGTTCGAACCTCACGTTTGTGGATCTTTCAAGAAACATGCTAGAAGGCTCAATCCCGGCGAAAATAGGTTCGCTTTACAATCTAATGAATTTAGACCTTTCTAGGAATAAACTCACCGGGGTCATACCACCAACCATCAGCAATGCCACCAAGCTACAATTTCTCATTCTTCAAGAAAATGAACTAGAGGGAAGCATACCCAGTGAGCTTGGACAATTGTCCAACATGATCGGCTTTACTGTTGGTAGCAATAGGCTCTCAGGTCAAATACCAGCATCAATCTTTAATCTTACTTTGCTCCGAGTGCTAGGCTTGTACGCAAATAGACTACAAATGGCGGCACTGCCACTTGACATTGGCCACACCCTCCCTAATCTCCAAAATATTACTTTGGGCCAAAACATGCTTGAAGGTCCTATCCCAGCGTCGCTAGGTAACATTTCAAGCCTGCAATTAATAGAGTTATCTAATAACAGTTTCACTGGAGAAATTCCTAGTTTCGGAAAGCTACAGAAACTTGTATACCTAAACCTTGCGGACAATAAGCTGGAGTCAAGTGACAGCCAAAGATGGGAATCTTTATATGGACTAACAAACTGCAGTCATCTAAAATCGCTCAGATTCAAGAATAATCAGCTGAAAGGAGTCATACCAAATTCGGTAGGTAAATTGTCCCCTAAACTTGAACTTCTACATCTGGGTGGAAACAATCTATCAGGAATAGTTCCTTCAAGCATAGGAAACCTTGATGGCTTAATAGATTTGGATCTTAGCACAAACAGTTTCAATGGTACAATTGAAGGATGGGTAGGAAGTCTTAAAAAACTACAATCTCTAGATCTTCATGGAAACAATTTCGTTGGAGCCATTCCACCCTCTTTTGGCAACCTTACTGAGCTAACATATCTGTATTTAGCAAAAAATGAATTTGAAGGGACCATACCTCCCATTCTCGGGAAACTTAAAAGACTCTCAGCCATGGACCTTAGCTATAATAATCTTCAAGGTGACATTCCTCCAGAACTCAGTGGGCTTACACAACTCCGTACACTGAATCTTTCATCTAACAGACTTACAGGAGAAATTCCTGTTGATCTGAGCCAGTGTCAAGACCTGGTAACCATCCAAATGGACCATAATAACTTGACGGGTGACATTCCAACCACTTTTGGTGACCTTATGAGCTTGAACATGCTCAGCCTTTCCTATAATGATTTATCAGGGGCCATCCCTGTAAGTCTTCAACATGTCAGCAAGTTGGACTTATCTCATAATCACCTCCAAGGAGAAATCCCACCAGAAGGAGTGTTTAGGAATGCCTCAGCCGTTTCGCTTGCTGGCAATTCAGAGCTTTGTGGAGGGGTGTCGGAACTGCATATGCCTCCATGCCCAGTTGCTTCTCAGAGAACTAAGATACGATATTACTTGATCAGGGTATTGATACCATTATTTGGCTTCATGTCGCTCCTATTATTGGTCTACTTTCTAGTCCTCGAGAGGAAAATGAGAAGAACAAGATATGAATCACAGGCTCCTTTGGGTGAGCATTTCCCTAAAGTTTCTTACAATGATCTGGTTGAAGCAACAAAGAACTTTTCCGAGTCTAACCTGCTTGGGAAAGGAAGCTATGGTACAGTGTACAAGGGAAACTTGGTGCAGCATAAGTTGGAAGTGGCAGTGAAGGTTTTTAACCTTGAGATGCAAGGCGCGGAGAGAAGCTTCATGCCAGAATGTGAAGCGCTGAGAAGCGTTCAACACCGGAATCTTGTTTCCATCATAACTGCATGTTCTACTGTTGATAGCGACGGTAGAGCTTTCAGGGCCCTAATTTACGAGTTCATGCCCAAGGGGAACTTGGACACGTGCCTTCATCACAAGGGGGACGGCAAAGCTGATAAGCATCTGACTTTAACTCAAAGAATCGGCATAGCTGTCAACATAGCAGATGCACTGGACTATTTACATAATGACAGCGAAAACCCCATCATCCATTGTGATCTGAAGCCCAGCAATATTCTTCTTGATGAGGACATGGTTGCTCATTTGGGGGATTTCGGTATTGCAAGGATTTTTCTTGATTCTGGGCTAAGACCAGCAAGCTCGACGAGTTCAATTGGTGTAAAAGGAACGATAGGCTATATCCCACCAGAGTACGGCGGGGGAGGCCGTATATCTACTTCTGGGGATGTCTACAGTTTTGGGATAGTGCTGCTGGAGATGTTGACTGGCAAAAGGCCAACAGATCCTATGTTTATGGATGGACTGGACATCGTCAACTTCGTGGGCAACAAGTTTCCACATCAAATACATGAAGTGATTGACATTTATCTAAAAGGAGAGTGCGAGTCAGAAGATTCGGTTCATCAGTGCCTCGTGTCTCTGCTGCAAGTAGCAGTCTCCTGCACACACTCCATCCCCGGCGAAAGAGCGAACATTAGAGATACAGCTAGCAAGCTCCAGGAAATTAAGGCGTCATATCTTGGAAGGAAGGCAAAGATAAATCCTTCAGTTTAATTACTTAACTACAACTACTACATATACTTCCCGGCAGAAAGAAAGAAAAACTACTGCATATAGGATAGGCCATGCAGATGTTTCAAACACTCACATCGAGTGTTGCTACTTTCCTGAAGGTGTTATCTTCTTTTTTTGTGAGTTCTTGATGTTAGAATATGATATGTGTTTAAAGATAGAGATAAAAAGTAGGGGTCGAGTAGGTTTAAACCATTTATTACTTGTTTTGTATTTCAAGTCTCTACCCCCTCATGTATTCTTAAAGTCGCCCTTTATCGAAAAGAAAAAAACCCTCATGTATTTTATATATACCTCATTTGAGGGTCAGATCAATACAACAC
CDS Sequence
  • >TraesCS6D02G206100.1
    ATGTCTGACCAATCCGTGAAACTCAACATGCTTCTTCTGCTGGCGTTTCTGCTGCTGTCTTATGGAGCTGGCAATGCCCGTTGCTCAACTGTTCATGCGAACATCACAGACATTCTCTCCTTGCTCCGATTCAAAAGGTCCACCCACGATCCAACAGGTTCCTTGAGGAACTGGAACCGAAGCATCCATTACTGCAAGTGGAATGGTGTCTCCTGCAGCTTACTGAATCCAGGGCGGGTGGCGGCTTTGGATCTCCCTGGCCAAAACTTGTCAGGTCAAGTCAACCCTTCTCTTGGGAACATAACGTTCCTTAAGCGCCTGAATTTGTCCTCCAATGGCTTCTCCGGCCAGTTACCTCCTCTGAGTCAGCTCCATGAGCTGACGCTTCTTGACATGAGCTCTAACTTATTCCAAGGGATAATCCCCGATTCACTCACACAATTTTCGAACCTACAGCTCCTGAATTTGTCCTACAATGGCTTCTCCGGCCAGTTACCTCCTCTGAACCAGCTTCCCGAGCTGGTGGTTCTTGACTTGAAATCCAATTTATTCCAAGGGATAATCCCCGACTCACTCACAAACTGTTCGAACCTCACGTTTGTGGATCTTTCAAGAAACATGCTAGAAGGCTCAATCCCGGCGAAAATAGGTTCGCTTTACAATCTAATGAATTTAGACCTTTCTAGGAATAAACTCACCGGGGTCATACCACCAACCATCAGCAATGCCACCAAGCTACAATTTCTCATTCTTCAAGAAAATGAACTAGAGGGAAGCATACCCAGTGAGCTTGGACAATTGTCCAACATGATCGGCTTTACTGTTGGTAGCAATAGGCTCTCAGGTCAAATACCAGCATCAATCTTTAATCTTACTTTGCTCCGAGTGCTAGGCTTGTACGCAAATAGACTACAAATGGCGGCACTGCCACTTGACATTGGCCACACCCTCCCTAATCTCCAAAATATTACTTTGGGCCAAAACATGCTTGAAGGTCCTATCCCAGCGTCGCTAGGTAACATTTCAAGCCTGCAATTAATAGAGTTATCTAATAACAGTTTCACTGGAGAAATTCCTAGTTTCGGAAAGCTACAGAAACTTGTATACCTAAACCTTGCGGACAATAAGCTGGAGTCAAGTGACAGCCAAAGATGGGAATCTTTATATGGACTAACAAACTGCAGTCATCTAAAATCGCTCAGATTCAAGAATAATCAGCTGAAAGGAGTCATACCAAATTCGGTAGGTAAATTGTCCCCTAAACTTGAACTTCTACATCTGGGTGGAAACAATCTATCAGGAATAGTTCCTTCAAGCATAGGAAACCTTGATGGCTTAATAGATTTGGATCTTAGCACAAACAGTTTCAATGGTACAATTGAAGGATGGGTAGGAAGTCTTAAAAAACTACAATCTCTAGATCTTCATGGAAACAATTTCGTTGGAGCCATTCCACCCTCTTTTGGCAACCTTACTGAGCTAACATATCTGTATTTAGCAAAAAATGAATTTGAAGGGACCATACCTCCCATTCTCGGGAAACTTAAAAGACTCTCAGCCATGGACCTTAGCTATAATAATCTTCAAGGTGACATTCCTCCAGAACTCAGTGGGCTTACACAACTCCGTACACTGAATCTTTCATCTAACAGACTTACAGGAGAAATTCCTGTTGATCTGAGCCAGTGTCAAGACCTGGTAACCATCCAAATGGACCATAATAACTTGACGGGTGACATTCCAACCACTTTTGGTGACCTTATGAGCTTGAACATGCTCAGCCTTTCCTATAATGATTTATCAGGGGCCATCCCTGTAAGTCTTCAACATGTCAGCAAGTTGGACTTATCTCATAATCACCTCCAAGGAGAAATCCCACCAGAAGGAGTGTTTAGGAATGCCTCAGCCGTTTCGCTTGCTGGCAATTCAGAGCTTTGTGGAGGGGTGTCGGAACTGCATATGCCTCCATGCCCAGTTGCTTCTCAGAGAACTAAGATACGATATTACTTGATCAGGGTATTGATACCATTATTTGGCTTCATGTCGCTCCTATTATTGGTCTACTTTCTAGTCCTCGAGAGGAAAATGAGAAGAACAAGATATGAATCACAGGCTCCTTTGGGTGAGCATTTCCCTAAAGTTTCTTACAATGATCTGGTTGAAGCAACAAAGAACTTTTCCGAGTCTAACCTGCTTGGGAAAGGAAGCTATGGTACAGTGTACAAGGGAAACTTGGTGCAGCATAAGTTGGAAGTGGCAGTGAAGGTTTTTAACCTTGAGATGCAAGGCGCGGAGAGAAGCTTCATGCCAGAATGTGAAGCGCTGAGAAGCGTTCAACACCGGAATCTTGTTTCCATCATAACTGCATGTTCTACTGTTGATAGCGACGGTAGAGCTTTCAGGGCCCTAATTTACGAGTTCATGCCCAAGGGGAACTTGGACACGTGCCTTCATCACAAGGGGGACGGCAAAGCTGATAAGCATCTGACTTTAACTCAAAGAATCGGCATAGCTGTCAACATAGCAGATGCACTGGACTATTTACATAATGACAGCGAAAACCCCATCATCCATTGTGATCTGAAGCCCAGCAATATTCTTCTTGATGAGGACATGGTTGCTCATTTGGGGGATTTCGGTATTGCAAGGATTTTTCTTGATTCTGGGCTAAGACCAGCAAGCTCGACGAGTTCAATTGGTGTAAAAGGAACGATAGGCTATATCCCACCAGAGTACGGCGGGGGAGGCCGTATATCTACTTCTGGGGATGTCTACAGTTTTGGGATAGTGCTGCTGGAGATGTTGACTGGCAAAAGGCCAACAGATCCTATGTTTATGGATGGACTGGACATCGTCAACTTCGTGGGCAACAAGTTTCCACATCAAATACATGAAGTGATTGACATTTATCTAAAAGGAGAGTGCGAGTCAGAAGATTCGGTTCATCAGTGCCTCGTGTCTCTGCTGCAAGTAGCAGTCTCCTGCACACACTCCATCCCCGGCGAAAGAGCGAACATTAGAGATACAGCTAGCAAGCTCCAGGAAATTAAGGCGTCATATCTTGGAAGGAAGGCAAAGATAAATCCTTCAGTTTAA
Protein Sequence
  • >TraesCS6D02G206100.1
    MSDQSVKLNMLLLLAFLLLSYGAGNARCSTVHANITDILSLLRFKRSTHDPTGSLRNWNRSIHYCKWNGVSCSLLNPGRVAALDLPGQNLSGQVNPSLGNITFLKRLNLSSNGFSGQLPPLSQLHELTLLDMSSNLFQGIIPDSLTQFSNLQLLNLSYNGFSGQLPPLNQLPELVVLDLKSNLFQGIIPDSLTNCSNLTFVDLSRNMLEGSIPAKIGSLYNLMNLDLSRNKLTGVIPPTISNATKLQFLILQENELEGSIPSELGQLSNMIGFTVGSNRLSGQIPASIFNLTLLRVLGLYANRLQMAALPLDIGHTLPNLQNITLGQNMLEGPIPASLGNISSLQLIELSNNSFTGEIPSFGKLQKLVYLNLADNKLESSDSQRWESLYGLTNCSHLKSLRFKNNQLKGVIPNSVGKLSPKLELLHLGGNNLSGIVPSSIGNLDGLIDLDLSTNSFNGTIEGWVGSLKKLQSLDLHGNNFVGAIPPSFGNLTELTYLYLAKNEFEGTIPPILGKLKRLSAMDLSYNNLQGDIPPELSGLTQLRTLNLSSNRLTGEIPVDLSQCQDLVTIQMDHNNLTGDIPTTFGDLMSLNMLSLSYNDLSGAIPVSLQHVSKLDLSHNHLQGEIPPEGVFRNASAVSLAGNSELCGGVSELHMPPCPVASQRTKIRYYLIRVLIPLFGFMSLLLLVYFLVLERKMRRTRYESQAPLGEHFPKVSYNDLVEATKNFSESNLLGKGSYGTVYKGNLVQHKLEVAVKVFNLEMQGAERSFMPECEALRSVQHRNLVSIITACSTVDSDGRAFRALIYEFMPKGNLDTCLHHKGDGKADKHLTLTQRIGIAVNIADALDYLHNDSENPIIHCDLKPSNILLDEDMVAHLGDFGIARIFLDSGLRPASSTSSIGVKGTIGYIPPEYGGGGRISTSGDVYSFGIVLLEMLTGKRPTDPMFMDGLDIVNFVGNKFPHQIHEVIDIYLKGECESEDSVHQCLVSLLQVAVSCTHSIPGERANIRDTASKLQEIKASYLGRKAKINPSV