Gene Details:

Functional Descriptions:

  • Here, we report positional cloning of Pairing homoeologous 2 (Ph2) and functional validation of the wheat DNA mismatch repair protein MSH7-3D as a key inhibitor of homoeologous recombination, thus solving a half-century-old question.
  • Ph2 encodes the mismatch repair protein MSH7-3D that inhibits wheat homoeologous recombination.
  • TaMSH7-3D reduces pollen viability but does not affect plant fertility.
  • TaMSH7-3D is expressed in anthers during meiotic prophase I.
  • Similar to Ph2 mutant phenotype, we show that mutating MSH7-3D induces a substantial increase in homoeologous recombination (up to 5.5 fold) in wheat-wild relative hybrids, which is also associated with a reduction in homologous recombination.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >TraesCS3D02G119400.1
    GCACGCAGGGCGCGAGACGCGAGACGGCGGGGAAAGCGGAAAACCCCCACCCATTTCCCCTCTCCACCGCGCTCGCATTTCCCGCTCGGAAATTCCCCCAAATAATTTCATCTCCCTCCCATTCCCCTCTCCGCTCCCCACCTCCCAGAGAACCCCGCTCCTCCCGCCCGGACGGAGCCACCTCGAGTCGAATCCCGGCCACCGCGATGCAGCCGCGGCGGCAGCAGCAGTCCATCCGCTCCTTCCTCCACCCGCGCCCGAGCCCGGCGCAGGAGGCGTCCGGCGCCGGCACGACCCCCGAGAGGCCCCCGCGGCCCCCCGCCGCGTCGTCGGTCGACGGCATCATGGAGCGGCTCGTGCGTCCGCCGTCGCAGGGGAGAAACAAAGATGCCACTCAGATTAGAAATGCTGAGAGAGCCTTACCTGGTAAAAATGAAGATACCTCAAATGAGCAGCCAAGTGCATCATTCCCTGTACGGTACAACGGCAAATATAGCAGAGGAACTGTGTTATTTGCAGAACATAGCACAGATACCACTCCGCCACAGGAGCCATTGAAGTTCTCTGCAAGGTCTTCCACAGATGAATTTGTTAGAGCAAGCACGCTGTTCCCTGAAATTGGTTCAGATCAAACTCTGCTTCAGGAGTGTCCGAAGAAGTTATCCTCAGAGTGCCCCAGCAACCAGTACGTTCAAGCTAATTCAGTGTTTGAAGCATTTGATGTACAAACTCCATCCCAGGATCCGTTAAAGAGAATCTTTTCTGGGCCTTTTCATGGAGCAGATACACCTTTATCAGAGTATCGTTCATATCCAATTCCTTTGCAGCATCCATCGAAAAAGTTGTCATCGGGCTCTTCTAGTGGTGAATACCTTAGAGCAGTGACACCGCTTGGACTTGATTCGAATGATACTCCTACAGCGAAACACTCAAAGAAGCTATTCTCTGGGTCTTCAGACCATTCATACATTAAAGCAACTAATTTGTTTCCGGAATTTGATTCAAATGGAACTCCGCTGCAGAACCACTCCAATAAGTTCTCAGTATCTATGAACAGTAAGAATATTGGAGCACCTGCTACACTGTTTCCGGAACTTGATTCTGTTCTTCTGAAACCAGAAACTCCAGTGACACAAGCAGTGGCTCCTCGTGGGAAGAGAGTTCAACAGGATCAATGCATGACTGCCAATAACTGTCAGTCTCCTTTGTGGGGTTCAAATAAGAAGGTGAAATCAGCTCATTGTTCTCCAGCTGGGAAAATGGTTCATGATGAAATGGCTGAAAGTGCACGTAGTAAATTTGAATGGCTGAATCCTTTGAATATCAGGGATGCAAATAAAAGGCGGCCAGATGACCCACTTTATGACAAGAGAACTCTTTTTATTCCACCTGATGCACTGAGAAAGATGTCAACATCTCAAAAGCAATACTGGACTATTAAGTGTAAATATATGGATGTTCTCCTCTTCTTCAAAGTGGGGAAGTTTTATGAGCTCTATGAAGTAGATGCTGAGATCGGCCAAAAGGAACTTGATTGGAAAATGACTATTAGTGGGGTGGGAAAATGCCGACAGGTTGGTATTTCAGAGAGTGGGATTGACAATGCTGTTGAAAAGCTTTTAGCTCGGGGATATAAAGTTGGAAGGATAGAACAAATGGAATCTGCAGCACAGGCGAAAGCTAGAGGACCAAATTCAGTTATTGAAAGAAAGTTAGCTCATGTATCCACACCGTCAACTGCAGCTGACAGCAACATAGGGCCTGATGCTGTTCATCTTCTTGCATTGAAAGAGGTTACTCTAGCTTCTAATGGTTCTCGGGTCTACGGATTTGCTTTTCTAGATTATGCTGCACTTAAAATCTGGGTTGGTTCACTTCAAGATGATGATTCTTCTGCAGCTTTGGGGGCTTTGCTGGTGCAGGTTTCCCCGAGGGAGATAATCTATGAATCCTCAGGCCTCTCAAGAGAAAGTCGTAAATCAATGATAAAATATGCCTCAGCAGGCTCTGTGAAAATGCAACTGACCCCACTACCTGGGACAGATTTCTCTGATGCCTCACAAATTCAAATGCTAGTACATTCTAAAGGATACTTTAAAGCATCAACAGATTCTTGGCTATCTGCATTGGATTATTCAGTGAATCAAGATGCAGTTATCTGTGCACTTGGTGGACTTATTGGTCATTTGACTAGACTTATGCTAGACGATGCTCTAAAAAATGGGGAAGTCTTACCTTACAATGTGTACCAAACTTGTTTAAGGATGGATGGTCAGACTCTTGTGAACCTGGAGATTTTCGGCAATAACTTTGATGGTGGCTCATCAGGTACTCTGTACAAGCACCTCAATCACTGCATAACCGCATCTGGTAAGCGGCTTTTAAGAAGATGGATATGCCATCCACTAAAAGATGTCGATGCTATAAATAGAAGGCTTGATATTGTTGAGGGTTTCATTCAACATTGTGGGGTAGGCTCTGTTACACTTGAGCATCTCCGGAAAATTCCTGACCTTGAGAGGTTACTTGGGCGAGTCAGATCTACTGTTGGGCTAACATCTGCTGTCCTGTTGCCTTTTGTTGGTGAAAAGATATTAAAGAGGCGGATTAAAACGTTTTGCATGCTTATCAAGGGCCTCCGGGTTGCAATTGACTTATTAAGTGCCTTGCGTAGAGAGGACCATGGCATTCCAGCGCTGTCAAAATCAGTTGATATTCCAACCCTGAGTTCTCTTGATGAATCAGTCCATCAGTTTGAAGAGGCTATACGCATTGACTTTGAACAGTACCAGGATCATGATATCAAAGACCATGATGCTACCACCTTGGCTAATTTAGTGGAACATTTTGTTGGAAAAGCTACCGAATGGTCTTTGGTAATCAATGCCATCAGCACTGTTGATGTCCTTAGGTCCTTTGCAGCAATGGCATTGTCATCATTTGGCACCATGTGCAGACCACGTATTCTGTTGAAAGACAAATCGCCTATACTTCGGATGAAGGGTCTATGGCATCCATATGCTTTTGCAGAAAGTGGAACTGGGCTTGTGCCAAACGATTTGTCTCTTGGCCAGGATTTATTGGGTCATAATCGCTTTGCATTGTTGTTGACTGGTCCAAATATGGGAGGAAAATCTACAATAATGCGCGCCACCTGCTTGGCTATCGTGCTTGCCCAGCTTGGCTGTTATGTCCCCTGCATATCATGTGAATTGACCCTTGCAGACTCCATCTTTACACGGCTAGGCGCAACGGATCGGATTATGTCTGGAGAAAGTACTTTTCTTGTCGAATGTAGTGAGACTGCATCTGTTCTTCAGAATGCAACTGAGGATTCTCTTGTCTTGCTTGATGAACTTGGCAGAGGAACTAGCACATTTGATGGATACGCGATTGCATATGCAGTATTCCGCCACCTGGTGGAACAGGTGCGATGCCGTCTGCTCTTTGCCACCCACTACCACCCTCTCACCAAGGAGTTCGCCTCCCACCCCCACGTGAGCCTCCAGCACATGGCCTGCATGCTGAGGCCAAGGAGCGGCGGCAACGGCGAGATGGAGCTCACCTTCCTCTACCGTCTTGTGTCAGGCGCCTCTCCGGAGAGCTACGGCCTGCAGGTCGCCACGATGGCGGGGATCCCAAAGTCCATAGTGGAGAAGGCGGCGGTCGCGGGCGAGATGATGAAGTCGAGGATCGCAGGGAACTTCAGGTCGAGCGAAGGGCGAGCGGAGTTCTCCACCCTCCACGAGGACTGGCTGCAGACGATCCTGGCGATCGGCGGCGTCAAGGACGCGCACCTGGACGAGGACACCATGGACACGATGTTCTGCGTCGCCCAGGAGCTCAAGTCTCATTTCAGGAAAGGAGGAAGCTGAGCGCTGAGAAGTCGCCACCGGTAATTATGCGTGGCACCATTAGATGTAGGTAGTCTGAAGGAGGAAGATGAGCACCGAGAAAGTCGCCGCTCACCATTAATCATCAGTGTTTTAATCCGTCCCAGTCGACGGCTTTGTATATATTTACCTCGCGTTTGTAATCACGCAAGCGCACCTGGGCCTGAGTTCATCTGAACTGTCAAAAACTTCATCTCGTAGTTTGTAATCACATGCACATTCCTAGTGATTAGTCGAGAGTTTTGACAAGGCTGGCAAGATCAAGCACCAACACCGCAAGTTCATCAAACTCCATTTGCCTGTATGGACAGCTACAAAGCCCACTTGCAGAGAAAAGGGCTTACCGCCATTTCTCGGACACAAGCATCCCCGTGCGCGCCGAGCTGTCCGTCCTCTCTTCCTACCATCCGC
CDS Sequence
  • >TraesCS3D02G119400.1
    ATGCAGCCGCGGCGGCAGCAGCAGTCCATCCGCTCCTTCCTCCACCCGCGCCCGAGCCCGGCGCAGGAGGCGTCCGGCGCCGGCACGACCCCCGAGAGGCCCCCGCGGCCCCCCGCCGCGTCGTCGGTCGACGGCATCATGGAGCGGCTCGTGCGTCCGCCGTCGCAGGGGAGAAACAAAGATGCCACTCAGATTAGAAATGCTGAGAGAGCCTTACCTGGTAAAAATGAAGATACCTCAAATGAGCAGCCAAGTGCATCATTCCCTGTACGGTACAACGGCAAATATAGCAGAGGAACTGTGTTATTTGCAGAACATAGCACAGATACCACTCCGCCACAGGAGCCATTGAAGTTCTCTGCAAGGTCTTCCACAGATGAATTTGTTAGAGCAAGCACGCTGTTCCCTGAAATTGGTTCAGATCAAACTCTGCTTCAGGAGTGTCCGAAGAAGTTATCCTCAGAGTGCCCCAGCAACCAGTACGTTCAAGCTAATTCAGTGTTTGAAGCATTTGATGTACAAACTCCATCCCAGGATCCGTTAAAGAGAATCTTTTCTGGGCCTTTTCATGGAGCAGATACACCTTTATCAGAGTATCGTTCATATCCAATTCCTTTGCAGCATCCATCGAAAAAGTTGTCATCGGGCTCTTCTAGTGGTGAATACCTTAGAGCAGTGACACCGCTTGGACTTGATTCGAATGATACTCCTACAGCGAAACACTCAAAGAAGCTATTCTCTGGGTCTTCAGACCATTCATACATTAAAGCAACTAATTTGTTTCCGGAATTTGATTCAAATGGAACTCCGCTGCAGAACCACTCCAATAAGTTCTCAGTATCTATGAACAGTAAGAATATTGGAGCACCTGCTACACTGTTTCCGGAACTTGATTCTGTTCTTCTGAAACCAGAAACTCCAGTGACACAAGCAGTGGCTCCTCGTGGGAAGAGAGTTCAACAGGATCAATGCATGACTGCCAATAACTGTCAGTCTCCTTTGTGGGGTTCAAATAAGAAGGTGAAATCAGCTCATTGTTCTCCAGCTGGGAAAATGGTTCATGATGAAATGGCTGAAAGTGCACGTAGTAAATTTGAATGGCTGAATCCTTTGAATATCAGGGATGCAAATAAAAGGCGGCCAGATGACCCACTTTATGACAAGAGAACTCTTTTTATTCCACCTGATGCACTGAGAAAGATGTCAACATCTCAAAAGCAATACTGGACTATTAAGTGTAAATATATGGATGTTCTCCTCTTCTTCAAAGTGGGGAAGTTTTATGAGCTCTATGAAGTAGATGCTGAGATCGGCCAAAAGGAACTTGATTGGAAAATGACTATTAGTGGGGTGGGAAAATGCCGACAGGTTGGTATTTCAGAGAGTGGGATTGACAATGCTGTTGAAAAGCTTTTAGCTCGGGGATATAAAGTTGGAAGGATAGAACAAATGGAATCTGCAGCACAGGCGAAAGCTAGAGGACCAAATTCAGTTATTGAAAGAAAGTTAGCTCATGTATCCACACCGTCAACTGCAGCTGACAGCAACATAGGGCCTGATGCTGTTCATCTTCTTGCATTGAAAGAGGTTACTCTAGCTTCTAATGGTTCTCGGGTCTACGGATTTGCTTTTCTAGATTATGCTGCACTTAAAATCTGGGTTGGTTCACTTCAAGATGATGATTCTTCTGCAGCTTTGGGGGCTTTGCTGGTGCAGGTTTCCCCGAGGGAGATAATCTATGAATCCTCAGGCCTCTCAAGAGAAAGTCGTAAATCAATGATAAAATATGCCTCAGCAGGCTCTGTGAAAATGCAACTGACCCCACTACCTGGGACAGATTTCTCTGATGCCTCACAAATTCAAATGCTAGTACATTCTAAAGGATACTTTAAAGCATCAACAGATTCTTGGCTATCTGCATTGGATTATTCAGTGAATCAAGATGCAGTTATCTGTGCACTTGGTGGACTTATTGGTCATTTGACTAGACTTATGCTAGACGATGCTCTAAAAAATGGGGAAGTCTTACCTTACAATGTGTACCAAACTTGTTTAAGGATGGATGGTCAGACTCTTGTGAACCTGGAGATTTTCGGCAATAACTTTGATGGTGGCTCATCAGGTACTCTGTACAAGCACCTCAATCACTGCATAACCGCATCTGGTAAGCGGCTTTTAAGAAGATGGATATGCCATCCACTAAAAGATGTCGATGCTATAAATAGAAGGCTTGATATTGTTGAGGGTTTCATTCAACATTGTGGGGTAGGCTCTGTTACACTTGAGCATCTCCGGAAAATTCCTGACCTTGAGAGGTTACTTGGGCGAGTCAGATCTACTGTTGGGCTAACATCTGCTGTCCTGTTGCCTTTTGTTGGTGAAAAGATATTAAAGAGGCGGATTAAAACGTTTTGCATGCTTATCAAGGGCCTCCGGGTTGCAATTGACTTATTAAGTGCCTTGCGTAGAGAGGACCATGGCATTCCAGCGCTGTCAAAATCAGTTGATATTCCAACCCTGAGTTCTCTTGATGAATCAGTCCATCAGTTTGAAGAGGCTATACGCATTGACTTTGAACAGTACCAGGATCATGATATCAAAGACCATGATGCTACCACCTTGGCTAATTTAGTGGAACATTTTGTTGGAAAAGCTACCGAATGGTCTTTGGTAATCAATGCCATCAGCACTGTTGATGTCCTTAGGTCCTTTGCAGCAATGGCATTGTCATCATTTGGCACCATGTGCAGACCACGTATTCTGTTGAAAGACAAATCGCCTATACTTCGGATGAAGGGTCTATGGCATCCATATGCTTTTGCAGAAAGTGGAACTGGGCTTGTGCCAAACGATTTGTCTCTTGGCCAGGATTTATTGGGTCATAATCGCTTTGCATTGTTGTTGACTGGTCCAAATATGGGAGGAAAATCTACAATAATGCGCGCCACCTGCTTGGCTATCGTGCTTGCCCAGCTTGGCTGTTATGTCCCCTGCATATCATGTGAATTGACCCTTGCAGACTCCATCTTTACACGGCTAGGCGCAACGGATCGGATTATGTCTGGAGAAAGTACTTTTCTTGTCGAATGTAGTGAGACTGCATCTGTTCTTCAGAATGCAACTGAGGATTCTCTTGTCTTGCTTGATGAACTTGGCAGAGGAACTAGCACATTTGATGGATACGCGATTGCATATGCAGTATTCCGCCACCTGGTGGAACAGGTGCGATGCCGTCTGCTCTTTGCCACCCACTACCACCCTCTCACCAAGGAGTTCGCCTCCCACCCCCACGTGAGCCTCCAGCACATGGCCTGCATGCTGAGGCCAAGGAGCGGCGGCAACGGCGAGATGGAGCTCACCTTCCTCTACCGTCTTGTGTCAGGCGCCTCTCCGGAGAGCTACGGCCTGCAGGTCGCCACGATGGCGGGGATCCCAAAGTCCATAGTGGAGAAGGCGGCGGTCGCGGGCGAGATGATGAAGTCGAGGATCGCAGGGAACTTCAGGTCGAGCGAAGGGCGAGCGGAGTTCTCCACCCTCCACGAGGACTGGCTGCAGACGATCCTGGCGATCGGCGGCGTCAAGGACGCGCACCTGGACGAGGACACCATGGACACGATGTTCTGCGTCGCCCAGGAGCTCAAGTCTCATTTCAGGAAAGGAGGAAGCTGA
Protein Sequence
  • >TraesCS3D02G119400.1
    MQPRRQQQSIRSFLHPRPSPAQEASGAGTTPERPPRPPAASSVDGIMERLVRPPSQGRNKDATQIRNAERALPGKNEDTSNEQPSASFPVRYNGKYSRGTVLFAEHSTDTTPPQEPLKFSARSSTDEFVRASTLFPEIGSDQTLLQECPKKLSSECPSNQYVQANSVFEAFDVQTPSQDPLKRIFSGPFHGADTPLSEYRSYPIPLQHPSKKLSSGSSSGEYLRAVTPLGLDSNDTPTAKHSKKLFSGSSDHSYIKATNLFPEFDSNGTPLQNHSNKFSVSMNSKNIGAPATLFPELDSVLLKPETPVTQAVAPRGKRVQQDQCMTANNCQSPLWGSNKKVKSAHCSPAGKMVHDEMAESARSKFEWLNPLNIRDANKRRPDDPLYDKRTLFIPPDALRKMSTSQKQYWTIKCKYMDVLLFFKVGKFYELYEVDAEIGQKELDWKMTISGVGKCRQVGISESGIDNAVEKLLARGYKVGRIEQMESAAQAKARGPNSVIERKLAHVSTPSTAADSNIGPDAVHLLALKEVTLASNGSRVYGFAFLDYAALKIWVGSLQDDDSSAALGALLVQVSPREIIYESSGLSRESRKSMIKYASAGSVKMQLTPLPGTDFSDASQIQMLVHSKGYFKASTDSWLSALDYSVNQDAVICALGGLIGHLTRLMLDDALKNGEVLPYNVYQTCLRMDGQTLVNLEIFGNNFDGGSSGTLYKHLNHCITASGKRLLRRWICHPLKDVDAINRRLDIVEGFIQHCGVGSVTLEHLRKIPDLERLLGRVRSTVGLTSAVLLPFVGEKILKRRIKTFCMLIKGLRVAIDLLSALRREDHGIPALSKSVDIPTLSSLDESVHQFEEAIRIDFEQYQDHDIKDHDATTLANLVEHFVGKATEWSLVINAISTVDVLRSFAAMALSSFGTMCRPRILLKDKSPILRMKGLWHPYAFAESGTGLVPNDLSLGQDLLGHNRFALLLTGPNMGGKSTIMRATCLAIVLAQLGCYVPCISCELTLADSIFTRLGATDRIMSGESTFLVECSETASVLQNATEDSLVLLDELGRGTSTFDGYAIAYAVFRHLVEQVRCRLLFATHYHPLTKEFASHPHVSLQHMACMLRPRSGGNGEMELTFLYRLVSGASPESYGLQVATMAGIPKSIVEKAAVAGEMMKSRIAGNFRSSEGRAEFSTLHEDWLQTILAIGGVKDAHLDEDTMDTMFCVAQELKSHFRKGGS