Gene Details:

Functional Descriptions:

  • Effects of the TaGW7 gene editing on grain size and weight.
  • With the increase in the functional TaGW7 copy number, the grain width and TGW decreased and grain length increased (Figure 3), but change in grain area was not obvious.
  • By editing the TaGW7 homoeologs in the B and D genomes, we showed that mutations in either of the two or both genomes increased the grain width and weight but reduced the grain length.
  • The effect sizes of mutations in the TaGW7 gene homoeologs coincided with the relative levels of their expression in the B and D genomes.
  • The effects of gene editing on grain morphology and weight traits were dosage dependent with the double-copy mutant showing larger effect than the respective single copy mutants.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >TraesCS2B02G202300.1
    GCTCTTATTTCACGCACCCAGCGCCCGCCTCCCTCTTAAAATCCCCGAGCCCCCCCTCGCTCCCTCTTCCCTCCATCCAACCCGTCCTCTCCCCTCAACAGGTCACGAGAACCGAGCCAGCCTCTGCATGTATGAGCCGCAATCATCATCATAAGTAGTAACATTCCTCCTCCGAACTCACAACGCCCATCCCAAGAGCCTGCAACACTTCTATATCTGCACCTGCATCCATCCACATACCGCCGCTAGCTTCTTGCCCGCAGCTGCTCTGCCTCTTCTTCTTCTCCTGCTGGTTTCAGAACCTTCTCCTGCTTTTGCTACCTCCGGTCGGTGCCGCTGCCGCACAGTGCGCGTGTGGCCTGCTGTGGGCGGTGAATGTGCGGCGGCCGTCTCGTTTGGGCCGGCGTTTGATCGGTTTGGGAGCGTCGGGAGATGCCGGTGGCGAGGAGGGTGCTCAACGCCTTCGCCGACGACATGCCGGAGTTCGACCGCCGCCAGATGGGCTGCATGGCCGGCATATTCCAGATCTTCGACCGCCAGCGGATGCTCACCGCGCGCCGCGCCGGCGCCGGCGGGCGACAGCCGCAGCAGAAGAGACTGCCCGCAGGCCGTACTCCACCAGAAAGCAGCAGCAATGTTCCAGTGCAGTGCTCGAGTACCCCCAAAATCATTCTGGAGAAAACACTCAGCAAAAGCATGACCGAGAACAGTAGCCTTTCGATAGAGTCATCAAGAGCTTCTTGTTCTTCCTCTTCATGCTCATCCTTCTCGTCGATCGACGGCAGCAAATCAGCCCAACAGGAGCTACCGTACATCAGCGAAGAACTCTTTCCGCAGAGGTCACCGAAGAGCTCACCAAGTCTGAAGGGCGCTGACATGGACACCAAGACCGCACAGCCGAACGTCGGTTTCAGAGACATCGTCAAGGACTCCATCAACCGGGACTCGGGAGGGTTAACTGTCATGACCACGGCGAATGGAGCAAGAAGGAACGCGCAGTACAAGGACTCGCCAAGGCCATTGCTGCTCTCCAAATCGATGGACGGAACCTACGTCATCAGCATCGACAGGACCACCAAGGCCGTCCCCGCAAACGTCGTCGAGTCCAGCAGGCGTTTCCCGGAGCAGTCGCGCTTCTCATGCGACGACCGGCGGCTCCTGCGACCGGCTGAAACCCAAGAAGCCAAGAAGCCTCCTTCCACAAGGGCCAAAGAGCTTCCTAGGCTGTCCTTGGACAGCAGGAAAGAGTCTCTGAGCCCAAGTTCACGTCTGAAGAGCTACAGTTACAGGAGAACCGATGACTCTCTCCTAGACGCTCTGAAGCCTCAAGACTCCCCAGGCCACAGGCGTTCCAACAGTGTCATCGCCAAGCTCATGGGGTTGGAAGAAGCACCAGATGCTATGGGGATGCTGATCGCTGATAGCTACGAGCCTGCAAGATCACCAAGAACAGCAGCACAAGCCACACGGAGCGAGCGTCCATCGCGTTCGCCCAGGAGAGATTGCCAGGATGCGTGTGGTTCGCTGCCGAAAAACGAGCCTTCAGCACTGAAAACAAGACCTCCTCCAAGAATTCTCACTGAAGCTGCTCCCTGGAGGCAGCAGGAGAGAGGCGGCAGCGCCACCAACAGCAAGGCTTCACAATGCCGAGACGCTGAGGTGAGGCCAAGAACCGCATCTCTCTATGCCGATATAGAAAGAAGGCTTGGTGGGCTCGAGTTCTCCGAGTGCAACAAAGACTTCAGAGCTCTCAGAATTCTGGGTGCATTGCATGCAAAGGATGCTAAGCACCAGAACAACGATGGTGATGCCGCATCAGTGGCCTCTCAGAGCCAAGAAGAGGATTCAGCCACCACCAGTTCCAGAAGCTTCCAGTCTCCCATTGTAGTCATGAAGCCAGCAAGAACCACCGAGAAGCCTGGGGTTGCTCCCCTTGCAGGGCTGAGAGGCCTCAGGAAGCTGCAGCCCAGAGATTCATCCTTCACTGACAAGAGCGAAGCCAGCACAAATGAGAAGATCCATTCTCGCGTTGCAAGGGCTCAATCCAAGTCCGACGAACCTACCAGCAGAGCGAGCTCGCCGAGACCTGCAGGGTCATCAAGCCCCAGGTTAGTGCAGAGGAAGGCAGAGTCGGAGAGGCGGTCTCGTCCCCCGGTCTCACCAAAGTCTCCAAGCAAGAAGGCAAATGAAGCAGCATCCCCAAGAGGAAGAACAAGATCAAAGCCTTCTCAAGTGAAGAGCAACCGTGACAATGAGGTATCGCAGAGTCCAGGAAGAAGGATCAGCTTGGCGAAGCAGATTGACGTCAGTATAATGGATTGTCAAAATCCTCTGGCTGCTAGATCATCGTTCATTGACCCAAAAACGCCGAGCCAAAAGAGCCCTTCTTCAATCCTGGGTTCAGATCACAAGATCCATTCACTGGAGAACGCCCTGAGCCCCGTGTCGGTCCTCGACACATCCTTCTACCATAAAAGTATCTCAGATTCATTTAAAGATGGCGAGACGCATACTTCAGACGAATGCTGGAATTCAAACAGCCTGCCGGACACGCCGCAGTCGAAGACGAGCAGTGAAGCCAGCCAGATCAAACCAGAAAACCTGGAGGCGCTCATTCAGAAACTTGAACAATTGCAATCGATGAACGATGAAGCTGCAAACTCCAAAGATCACCAGTATATCTACGAGATACTCTTAGCATCTGGTCTTCTGCACAAGGAACTTAGCTTCGCAGCGATGCCTGCACAACTTTCGCCATCCAGTTGCCCGATCAATCCAGAGCTCTTCCTCATTCTAGAGCAAACAAAGCCGCACTTCATTTCAGCGACTCAAGCTGTTACCGGAGCCAAGAAGAGTTCTGACCCTAACATGGAGAAGCTTCATCGCAGAATTGTGTTCGATCTGGTAAATGAAATTATAGCTCAGAAGATGAACATGTATAGCTCTGCAAGTGGAACGGCGAAACTTCTTCGATCAAGGAAGTTAAGTGGGTGGCGTCTATTCAAGGAGTTGTGCACAGAGGTCGACAGGCTCTTATCCGAGAGTTCGGCAGCGAAATGCTCCGAAGAGGATGAGGATGAAAACATACCGTTATCTGAGGATGCATTGTATGAGATGAAAGATTGGGGTAGCCCTGAAGGTGAGCTACAAGGCATGGTTTTGGACATTGAAAGATCCATCTTCCGGGACCTCATTGACGAGGTCATAGGTGGCGAGGCTACAGAGAGGATGCAAGCTGGACAATGGAAATTGCGTAGGCAGCTGTCTTTCAGTAGTATAAACTGAATACGACTGGCGATAGTTGTTGTTTCCATTAGGAAGCTTAGCCACTAGTATAAATGTAACCTTTGTGTTTTTCTGAAGACTGAATTATATAGAAGAAATATAATTATTCCTGG
CDS Sequence
  • >TraesCS2B02G202300.1
    ATGCCGGTGGCGAGGAGGGTGCTCAACGCCTTCGCCGACGACATGCCGGAGTTCGACCGCCGCCAGATGGGCTGCATGGCCGGCATATTCCAGATCTTCGACCGCCAGCGGATGCTCACCGCGCGCCGCGCCGGCGCCGGCGGGCGACAGCCGCAGCAGAAGAGACTGCCCGCAGGCCGTACTCCACCAGAAAGCAGCAGCAATGTTCCAGTGCAGTGCTCGAGTACCCCCAAAATCATTCTGGAGAAAACACTCAGCAAAAGCATGACCGAGAACAGTAGCCTTTCGATAGAGTCATCAAGAGCTTCTTGTTCTTCCTCTTCATGCTCATCCTTCTCGTCGATCGACGGCAGCAAATCAGCCCAACAGGAGCTACCGTACATCAGCGAAGAACTCTTTCCGCAGAGGTCACCGAAGAGCTCACCAAGTCTGAAGGGCGCTGACATGGACACCAAGACCGCACAGCCGAACGTCGGTTTCAGAGACATCGTCAAGGACTCCATCAACCGGGACTCGGGAGGGTTAACTGTCATGACCACGGCGAATGGAGCAAGAAGGAACGCGCAGTACAAGGACTCGCCAAGGCCATTGCTGCTCTCCAAATCGATGGACGGAACCTACGTCATCAGCATCGACAGGACCACCAAGGCCGTCCCCGCAAACGTCGTCGAGTCCAGCAGGCGTTTCCCGGAGCAGTCGCGCTTCTCATGCGACGACCGGCGGCTCCTGCGACCGGCTGAAACCCAAGAAGCCAAGAAGCCTCCTTCCACAAGGGCCAAAGAGCTTCCTAGGCTGTCCTTGGACAGCAGGAAAGAGTCTCTGAGCCCAAGTTCACGTCTGAAGAGCTACAGTTACAGGAGAACCGATGACTCTCTCCTAGACGCTCTGAAGCCTCAAGACTCCCCAGGCCACAGGCGTTCCAACAGTGTCATCGCCAAGCTCATGGGGTTGGAAGAAGCACCAGATGCTATGGGGATGCTGATCGCTGATAGCTACGAGCCTGCAAGATCACCAAGAACAGCAGCACAAGCCACACGGAGCGAGCGTCCATCGCGTTCGCCCAGGAGAGATTGCCAGGATGCGTGTGGTTCGCTGCCGAAAAACGAGCCTTCAGCACTGAAAACAAGACCTCCTCCAAGAATTCTCACTGAAGCTGCTCCCTGGAGGCAGCAGGAGAGAGGCGGCAGCGCCACCAACAGCAAGGCTTCACAATGCCGAGACGCTGAGGTGAGGCCAAGAACCGCATCTCTCTATGCCGATATAGAAAGAAGGCTTGGTGGGCTCGAGTTCTCCGAGTGCAACAAAGACTTCAGAGCTCTCAGAATTCTGGGTGCATTGCATGCAAAGGATGCTAAGCACCAGAACAACGATGGTGATGCCGCATCAGTGGCCTCTCAGAGCCAAGAAGAGGATTCAGCCACCACCAGTTCCAGAAGCTTCCAGTCTCCCATTGTAGTCATGAAGCCAGCAAGAACCACCGAGAAGCCTGGGGTTGCTCCCCTTGCAGGGCTGAGAGGCCTCAGGAAGCTGCAGCCCAGAGATTCATCCTTCACTGACAAGAGCGAAGCCAGCACAAATGAGAAGATCCATTCTCGCGTTGCAAGGGCTCAATCCAAGTCCGACGAACCTACCAGCAGAGCGAGCTCGCCGAGACCTGCAGGGTCATCAAGCCCCAGGTTAGTGCAGAGGAAGGCAGAGTCGGAGAGGCGGTCTCGTCCCCCGGTCTCACCAAAGTCTCCAAGCAAGAAGGCAAATGAAGCAGCATCCCCAAGAGGAAGAACAAGATCAAAGCCTTCTCAAGTGAAGAGCAACCGTGACAATGAGGTATCGCAGAGTCCAGGAAGAAGGATCAGCTTGGCGAAGCAGATTGACGTCAGTATAATGGATTGTCAAAATCCTCTGGCTGCTAGATCATCGTTCATTGACCCAAAAACGCCGAGCCAAAAGAGCCCTTCTTCAATCCTGGGTTCAGATCACAAGATCCATTCACTGGAGAACGCCCTGAGCCCCGTGTCGGTCCTCGACACATCCTTCTACCATAAAAGTATCTCAGATTCATTTAAAGATGGCGAGACGCATACTTCAGACGAATGCTGGAATTCAAACAGCCTGCCGGACACGCCGCAGTCGAAGACGAGCAGTGAAGCCAGCCAGATCAAACCAGAAAACCTGGAGGCGCTCATTCAGAAACTTGAACAATTGCAATCGATGAACGATGAAGCTGCAAACTCCAAAGATCACCAGTATATCTACGAGATACTCTTAGCATCTGGTCTTCTGCACAAGGAACTTAGCTTCGCAGCGATGCCTGCACAACTTTCGCCATCCAGTTGCCCGATCAATCCAGAGCTCTTCCTCATTCTAGAGCAAACAAAGCCGCACTTCATTTCAGCGACTCAAGCTGTTACCGGAGCCAAGAAGAGTTCTGACCCTAACATGGAGAAGCTTCATCGCAGAATTGTGTTCGATCTGGTAAATGAAATTATAGCTCAGAAGATGAACATGTATAGCTCTGCAAGTGGAACGGCGAAACTTCTTCGATCAAGGAAGTTAAGTGGGTGGCGTCTATTCAAGGAGTTGTGCACAGAGGTCGACAGGCTCTTATCCGAGAGTTCGGCAGCGAAATGCTCCGAAGAGGATGAGGATGAAAACATACCGTTATCTGAGGATGCATTGTATGAGATGAAAGATTGGGGTAGCCCTGAAGGTGAGCTACAAGGCATGGTTTTGGACATTGAAAGATCCATCTTCCGGGACCTCATTGACGAGGTCATAGGTGGCGAGGCTACAGAGAGGATGCAAGCTGGACAATGGAAATTGCGTAGGCAGCTGTCTTTCAGTAGTATAAACTGA
Protein Sequence
  • >TraesCS2B02G202300.1
    MPVARRVLNAFADDMPEFDRRQMGCMAGIFQIFDRQRMLTARRAGAGGRQPQQKRLPAGRTPPESSSNVPVQCSSTPKIILEKTLSKSMTENSSLSIESSRASCSSSSCSSFSSIDGSKSAQQELPYISEELFPQRSPKSSPSLKGADMDTKTAQPNVGFRDIVKDSINRDSGGLTVMTTANGARRNAQYKDSPRPLLLSKSMDGTYVISIDRTTKAVPANVVESSRRFPEQSRFSCDDRRLLRPAETQEAKKPPSTRAKELPRLSLDSRKESLSPSSRLKSYSYRRTDDSLLDALKPQDSPGHRRSNSVIAKLMGLEEAPDAMGMLIADSYEPARSPRTAAQATRSERPSRSPRRDCQDACGSLPKNEPSALKTRPPPRILTEAAPWRQQERGGSATNSKASQCRDAEVRPRTASLYADIERRLGGLEFSECNKDFRALRILGALHAKDAKHQNNDGDAASVASQSQEEDSATTSSRSFQSPIVVMKPARTTEKPGVAPLAGLRGLRKLQPRDSSFTDKSEASTNEKIHSRVARAQSKSDEPTSRASSPRPAGSSSPRLVQRKAESERRSRPPVSPKSPSKKANEAASPRGRTRSKPSQVKSNRDNEVSQSPGRRISLAKQIDVSIMDCQNPLAARSSFIDPKTPSQKSPSSILGSDHKIHSLENALSPVSVLDTSFYHKSISDSFKDGETHTSDECWNSNSLPDTPQSKTSSEASQIKPENLEALIQKLEQLQSMNDEAANSKDHQYIYEILLASGLLHKELSFAAMPAQLSPSSCPINPELFLILEQTKPHFISATQAVTGAKKSSDPNMEKLHRRIVFDLVNEIIAQKMNMYSSASGTAKLLRSRKLSGWRLFKELCTEVDRLLSESSAAKCSEEDEDENIPLSEDALYEMKDWGSPEGELQGMVLDIERSIFRDLIDEVIGGEATERMQAGQWKLRRQLSFSSIN