Gene Details:

Functional Descriptions:

  • Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2.
  • TaASN2 encodes the glutamine amidotransferase domain of the protein.
  • Free asparagine concentrations in the grain of plants carrying edits in all six TaASN2 alleles were substantially reduced compared with wildtype, with one plant showing a more than 90% reduction in the T2 seeds.
  • TaASN2 gene in wheat cv. Cadenza using CRISPR/Cas9, to reduce asparagine synthetase gene expression in the grain without affecting its expression elsewhere in the plant.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >TraesCS1A02G382800.2
    ATGCTGGATGGCATGTTCTCGTTTGTGCTTCTTGACACACGTGATAAAAGCTTCATTGCTGCCCGTGATGCTATTGGCATCTGTCCTTTGTACATGGGCTGGGGTCTTGATGAGATGAAGGCATTGAGTGATGATTGCGAGCGCTTCATATCGTTCCCTCCTGGACACTTGTACTCAAGCAAAACAGGTGGCCTAAGGAGGTGGTACAACCCCCCATGGTTTTCAGAAAGCATTCCCTCAGCCCCCTATGATCCTCTCCTCATCCGAGAGAGTTTTGAGAAGGCTGTTATTAAGAGGCTAATGACTGATGTGCCATTTGGTGTTCTCTTGTCTGGTGGGCTTGACTCTTCTTTGGTGGCATCTGTTGTTTCACGCTACTTGGCAGAAACAAAAGTTGCTAGGCAGTGGGGAAACAAACTGCACACCTTTTGCATCGGTTTGAAGGGTTCTCCTGATCTTAAAGCTGCTAAGGAAGTTGCTGACTACCTTGGCACAGTCCATCATGAATTACACTTTACAGTGCAGGAGGGCATTGATGCTTTGGAAGAAGTTATATATCACATCGAGACGTATGACGTAACGACCATTAGAGCAAGTACCCCGATGTTTCTAATGTCTCGGAAAATCAAATCGTTGGGTGTGAAGATGGTTCTTTCGGGTGAAGGTTCCGATGAAATATTTGGTGGTTATCTTTATTTTCATAAGGCACCAAACAAAAAGGAACTCCATGAGGAAACATGTCGGAAGATAAAAGCTCTCCATTTATATGATTGTTTGAGAGCGAACAAAGCAACTTCTGCCTGGGGTCTCGAGGCTCGTGTTCCATTCCTCGACAAAAACTTCATCAATGTAGCAATGGACCTGGATCCGGAATGTAAGATGATAAGGCGTGATCTTGGTCGGATCGAGAAATGGGTCCTGCGTAATGCATTTGATGATGAGAAGAAGCCCTATTTACCCAAGCACATTCTTTACAGGCAAAAAGAACAGTTCAGCGATGGTGTTGGGTACAGTTGGATTGATGGATTGAAGGACCATGCTAACGCACATGTGTCAGATTCCATGATGACGAACGCCAGCTTTGTTTACCCTGAAAACACACCCACAACAAAAGAAGCCTACTATTATAGGACAGTATTTGAGAAGTTCTATCCTAAGAATGCTGCTAGGCTAACAGTGCCAGGAGGTCCCAGCGTTGCATGCAGCACCGCGAAAGCTGTTGAATGGGACACCGCCTGGTCGAAGCTCCTTGACCCCTCTGGCCGTGCTGCTCTCGGTGTGCATGACGCGGCATACGAAGAAGAAAAGGCTCCTGCATCGGCCGATCCCGTCTTCCGTCCACCAGCCCACGGGGAGAGCATCCTAGTCGAAACTGGTGTTCCAGCAGCTGCTGTTTAACCTTCCATTCCATGGTTCCATAAAATGCTCGAGAAAATGTTGTTGCTTAGTTTAATTCTAGCTTCCTTGCAACTTGTCCGTAGCGGTCATTCAGTGTAGAAGTCGCTTTGTTCCATTTTCCTTGTTGCTTGCTGTTATGTTTTGCTTTTCGCATGTAATTTACTAAGTTTATGTGGTGAGCATTGCATTGTAAATATTTCACCATGGTTGTACCATCCGAGTTGCTCAATGTCTGGTTTGCAAACTGCAAAGTTTCATTAATAAATTCCAAATGTTGGTTTCTATATTTCTTTTTG
  • >TraesCS1A02G382800.1
    ATGCTGGATGGCATGTTCTCGTTTGTGCTTCTTGACACACGTGATAAAAGCTTCATTGCTGCCCGTGATGCTATTGGCATCTGTCCTTTGTACATGGGCTGGGGTCTTGATGAGATGAAGGCATTGAGTGATGATTGCGAGCGCTTCATATCGTTCCCTCCTGGACACTTGTACTCAAGCAAAACAGGTGGCCTAAGGAGGTGGTACAACCCCCCATGGTTTTCAGAAAGCATTCCCTCAGCCCCCTATGATCCTCTCCTCATCCGAGAGAGTTTTGAGAAGGCTGTTATTAAGAGGCTAATGACTGATGTGCCATTTGGTGTTCTCTTGTCTGGTGGGCTTGACTCTTCTTTGGTGGCATCTGTTGTTTCACGCTACTTGGCAGAAACAAAAGTTGCTAGGCAGTGGGGAAACAAACTGCACACCTTTTGCATCGGTTTGAAGGGTTCTCCTGATCTTAAAGCTGCTAAGGAAGTTGCTGACTACCTTGGCACAGTCCATCATGAATTACACTTTACAGTGCAGGAGGGCATTGATGCTTTGGAAGAAGTTATATATCACATCGAGACGTATGACGTAACGACCATTAGAGCAAGTACCCCGATGTTTCTAATGTCTCGGAAAATCAAATCGTTGGGTGTGAAGATGGTTCTTTCGGGTGAAGGTTCCGATGAAATATTTGGTGGTTATCTTTATTTTCATAAGGCACCAAACAAAAAGGAACTCCATGAGGAAACATGTCGGAAGATAAAAGCTCTCCATTTATATGATTGTTTGAGAGCGAACAAAGCAACTTCTGCCTGGGGTCTCGAGGCTCGTGTTCCATTCCTCGACAAAAACTTCATCAATGTAGCAATGGACCTGGATCCGGAATGTAAGATGATAAGGCGTGATCTTGGTCGGATCGAGAAATGGGTCCTGCGTAATGCATTTGATGATGAGAAGAAGCCCTATTTACCCAAGCACATTCTTTACAGGCAAAAAGAACAGTTCAGCGATGGTGTTGGGTACAGTTGGATTGATGGATTGAAGGACCATGCTAACGCACATGTGTCAGATTCCATGATGACGAACGCCAGCTTTGTTTACCCTGAAAACACACCCACAACAAAAGAAGCCTACTATTATAGGACAGTATTTGAGAAGTTCTATCCTAAGAATGCTGCTAGGCTAACAGTGCCAGGAGGTCCCAGCGTTGCATGCAGCACCGCGAAAGCTGTTGAATGGGACACCGCCTGGTCGAAGCTCCTTGACCCCTCTGGCCGTGCTGCTCTCGGTGTGCATGACGCGGCATACGAAGAAGAAAAGGCTCCTGCATCGGCCGATCCCGTCTTCCGTCCACCAGCCCACGGGGAGAGCATCCTAGTCGAAACTGGTGTTCCAGCAGCTGCTGTTTAACCTTCCATTCCATGGTTCCATAAAATGCTCGAGAAAATGTTGTTGCTTAGTTTAATTCTAGCTTCCTTGCAACTTGTCCGTAGCGGTCATTCAGTGTAGAAGTCGCTTTGTTCCATTTTCCTTGTTGCTTGCTGTTATGTTTTGCTTTTCGCATGTAATTTACTAAGTTTATGTGGTGAGCATTGCATTGTAAATATTTCACCATGGTTGTACCATCCGAGTTGCTCAATGTCTGGTTTGCAAACTGCAAAGTTTCATTAATAAATTCCAAATGTTGGTTTCTATATTTCTTTTTG
CDS Sequence
  • >TraesCS1A02G382800.2
    ATGCTGGATGGCATGTTCTCGTTTGTGCTTCTTGACACACGTGATAAAAGCTTCATTGCTGCCCGTGATGCTATTGGCATCTGTCCTTTGTACATGGGCTGGGGTCTTGATGAGATGAAGGCATTGAGTGATGATTGCGAGCGCTTCATATCGTTCCCTCCTGGACACTTGTACTCAAGCAAAACAGGTGGCCTAAGGAGGTGGTACAACCCCCCATGGTTTTCAGAAAGCATTCCCTCAGCCCCCTATGATCCTCTCCTCATCCGAGAGAGTTTTGAGAAGGCTGTTATTAAGAGGCTAATGACTGATGTGCCATTTGGTGTTCTCTTGTCTGGTGGGCTTGACTCTTCTTTGGTGGCATCTGTTGTTTCACGCTACTTGGCAGAAACAAAAGTTGCTAGGCAGTGGGGAAACAAACTGCACACCTTTTGCATCGGTTTGAAGGGTTCTCCTGATCTTAAAGCTGCTAAGGAAGTTGCTGACTACCTTGGCACAGTCCATCATGAATTACACTTTACAGTGCAGGAGGGCATTGATGCTTTGGAAGAAGTTATATATCACATCGAGACGTATGACGTAACGACCATTAGAGCAAGTACCCCGATGTTTCTAATGTCTCGGAAAATCAAATCGTTGGGTGTGAAGATGGTTCTTTCGGGTGAAGGTTCCGATGAAATATTTGGTGGTTATCTTTATTTTCATAAGGCACCAAACAAAAAGGAACTCCATGAGGAAACATGTCGGAAGATAAAAGCTCTCCATTTATATGATTGTTTGAGAGCGAACAAAGCAACTTCTGCCTGGGGTCTCGAGGCTCGTGTTCCATTCCTCGACAAAAACTTCATCAATGTAGCAATGGACCTGGATCCGGAATGTAAGATGATAAGGCGTGATCTTGGTCGGATCGAGAAATGGGTCCTGCGTAATGCATTTGATGATGAGAAGAAGCCCTATTTACCCAAGCACATTCTTTACAGGCAAAAAGAACAGTTCAGCGATGGTGTTGGGTACAGTTGGATTGATGGATTGAAGGACCATGCTAACGCACATGTGTCAGATTCCATGATGACGAACGCCAGCTTTGTTTACCCTGAAAACACACCCACAACAAAAGAAGCCTACTATTATAGGACAGTATTTGAGAAGTTCTATCCTAAGAATGCTGCTAGGCTAACAGTGCCAGGAGGTCCCAGCGTTGCATGCAGCACCGCGAAAGCTGTTGAATGGGACACCGCCTGGTCGAAGCTCCTTGACCCCTCTGGCCGTGCTGCTCTCGGTGTGCATGACGCGGCATACGAAGAAGAAAAGGCTCCTGCATCGGCCGATCCCGTCTTCCGTCCACCAGCCCACGGGGAGAGCATCCTAGTCGAAACTGGTGTTCCAGCAGCTGCTGTTTAA
  • >TraesCS1A02G382800.1
    ATGCTGGATGGCATGTTCTCGTTTGTGCTTCTTGACACACGTGATAAAAGCTTCATTGCTGCCCGTGATGCTATTGGCATCTGTCCTTTGTACATGGGCTGGGGTCTTGATGAGATGAAGGCATTGAGTGATGATTGCGAGCGCTTCATATCGTTCCCTCCTGGACACTTGTACTCAAGCAAAACAGGTGGCCTAAGGAGGTGGTACAACCCCCCATGGTTTTCAGAAAGCATTCCCTCAGCCCCCTATGATCCTCTCCTCATCCGAGAGAGTTTTGAGAAGGCTGTTATTAAGAGGCTAATGACTGATGTGCCATTTGGTGTTCTCTTGTCTGGTGGGCTTGACTCTTCTTTGGTGGCATCTGTTGTTTCACGCTACTTGGCAGAAACAAAAGTTGCTAGGCAGTGGGGAAACAAACTGCACACCTTTTGCATCGGTTTGAAGGGTTCTCCTGATCTTAAAGCTGCTAAGGAAGTTGCTGACTACCTTGGCACAGTCCATCATGAATTACACTTTACAGTGCAGGAGGGCATTGATGCTTTGGAAGAAGTTATATATCACATCGAGACGTATGACGTAACGACCATTAGAGCAAGTACCCCGATGTTTCTAATGTCTCGGAAAATCAAATCGTTGGGTGTGAAGATGGTTCTTTCGGGTGAAGGTTCCGATGAAATATTTGGTGGTTATCTTTATTTTCATAAGGCACCAAACAAAAAGGAACTCCATGAGGAAACATGTCGGAAGATAAAAGCTCTCCATTTATATGATTGTTTGAGAGCGAACAAAGCAACTTCTGCCTGGGGTCTCGAGGCTCGTGTTCCATTCCTCGACAAAAACTTCATCAATGTAGCAATGGACCTGGATCCGGAATGTAAGATGATAAGGCGTGATCTTGGTCGGATCGAGAAATGGGTCCTGCGTAATGCATTTGATGATGAGAAGAAGCCCTATTTACCCAAGCACATTCTTTACAGGCAAAAAGAACAGTTCAGCGATGGTGTTGGGTACAGTTGGATTGATGGATTGAAGGACCATGCTAACGCACATGTGTCAGATTCCATGATGACGAACGCCAGCTTTGTTTACCCTGAAAACACACCCACAACAAAAGAAGCCTACTATTATAGGACAGTATTTGAGAAGTTCTATCCTAAGAATGCTGCTAGGCTAACAGTGCCAGGAGGTCCCAGCGTTGCATGCAGCACCGCGAAAGCTGTTGAATGGGACACCGCCTGGTCGAAGCTCCTTGACCCCTCTGGCCGTGCTGCTCTCGGTGTGCATGACGCGGCATACGAAGAAGAAAAGGCTCCTGCATCGGCCGATCCCGTCTTCCGTCCACCAGCCCACGGGGAGAGCATCCTAGTCGAAACTGGTGTTCCAGCAGCTGCTGTTTAA
Protein Sequence
  • >TraesCS1A02G382800.2
    MLDGMFSFVLLDTRDKSFIAARDAIGICPLYMGWGLDEMKALSDDCERFISFPPGHLYSSKTGGLRRWYNPPWFSESIPSAPYDPLLIRESFEKAVIKRLMTDVPFGVLLSGGLDSSLVASVVSRYLAETKVARQWGNKLHTFCIGLKGSPDLKAAKEVADYLGTVHHELHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPNKKELHEETCRKIKALHLYDCLRANKATSAWGLEARVPFLDKNFINVAMDLDPECKMIRRDLGRIEKWVLRNAFDDEKKPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANAHVSDSMMTNASFVYPENTPTTKEAYYYRTVFEKFYPKNAARLTVPGGPSVACSTAKAVEWDTAWSKLLDPSGRAALGVHDAAYEEEKAPASADPVFRPPAHGESILVETGVPAAAV
  • >TraesCS1A02G382800.1
    MLDGMFSFVLLDTRDKSFIAARDAIGICPLYMGWGLDEMKALSDDCERFISFPPGHLYSSKTGGLRRWYNPPWFSESIPSAPYDPLLIRESFEKAVIKRLMTDVPFGVLLSGGLDSSLVASVVSRYLAETKVARQWGNKLHTFCIGLKGSPDLKAAKEVADYLGTVHHELHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPNKKELHEETCRKIKALHLYDCLRANKATSAWGLEARVPFLDKNFINVAMDLDPECKMIRRDLGRIEKWVLRNAFDDEKKPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANAHVSDSMMTNASFVYPENTPTTKEAYYYRTVFEKFYPKNAARLTVPGGPSVACSTAKAVEWDTAWSKLLDPSGRAALGVHDAAYEEEKAPASADPVFRPPAHGESILVETGVPAAAV