Gene Details:

Functional Descriptions:

  • Expression indicated that knock-out of TaARE1 homoeologs could stimulate nitrate transport rather than ammonium transport upon N deprivation.
  • Three TaARE1 homoeologs resulted in more active nitrate transport than ammonium transport upon N deprivation, and the different TaARE1 homoeologs have diverged in this function.
  • We isolated and characterized three TaARE1 homeologs in an elite Chinese winter wheat cv ZhengMai 7698 and then generated a series of transgene-free mutant lines either with partial or triple null taare1 alleles through CRISPR/Cas9-mediated targeted mutagenesis of TaARE1.
  • All transgene-free mutant lines (TaARE1) showed enhanced tolerance to nitrogen starvation, and delayed senescence and increased grain yield in a field experiment.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >TraesCS7D02G283700.1
    GGTCACCGCAGCGCGGTCTGGGGCTATACAATTCTCTGGCTCCTGGTTGTTGCGATTCGCAGATTCTGCAAAGAAGAGGGGTTGACTGTATTCCGGCCTTCCGGGAGTTCGAAAATTCTTGGTTTCTTGATGTGGATACTGCTACCTTGCAATGACAACGGGTGGCAAGAAGGGAATTCTGTGAGGCTGTGAGCACATGGTTGCCAGAGGTGCGTCCGCCCCCGTTCTGTAGCCCGGAAGATGTGGTCTTCGTTGCATGACCTTTAATGAGTTGCTACGTGGTCAGCTCTAGCGGCGTTGCGGTCTGGTTCGCCGTAGAGGAGAGGATCGGGCACCGGAGGGTTTGCGCATGCAAGATGTTCGATGTCGGTCCCCAGAGGAGGAGGGTGGGGAGGCGCCTGGTGGGTTTTGCCAAGAAGAGGAGGCGTTCCAAGAGGCAGCAGCCATGGTGGAAGGCGTGGTTCTCTGATTGGAACGATGAGGAAGAGAGCCTCGCCGGCTGGAGGGAGGATGATGAATTGCTCCAGCAGGTTGTTAGCAACGAAGACCTGTCGGAGGATGACAAGTTTCAGACGTGGAAGAGCAAGGCCGAGGCGATTGTCGACCTGCGGGAAGCCCAGCAGGATGCCGAAAATGCAGAAGGGCGGTCATGGGAGGATTGGATAGGTTGGGGCAGCACGTCCGGCGATGGTGATTGGGGCGGGGGTGGGAGCTTGTCGGACCAGATAACGGATGATCCGACGGAGATAGTGAGGGACAAGGGCATCGCTGAAGCTTTTAGGGACTCTATTGATGAAGATTACAACGACATGTTGTTTGAGGACCGGGTTTTTCTATACGCTTCGACGAAATCGGCCAAATTCCTAGCATTGTTGATCGTTGTTCCATGGGTGTTGGATCTTCTAGTACATGACTATGTTATGATGCCATTTCTAGACAGGTATGTCGAGAAGGTACCACTCGCCGCTGAAATGCTTGATGTAAGACGCAGCCAGAAGATTCAGATGATAAAGGACCTAAATATTGAGAAAGCAAGATTCCGTTTTGAAGTAGAGATTGGTAAATCTCCTCCACTTTCCGATGAGGAGTTCTGGTCAGAGTTGCGGGAAAAAGCGGTAGAGCTGAGGGATGAATGGAGATTAGAAAACCGACAAGCATTTGCAAATATCTGGTCTGATATGGTTTATGGGGTTGCCCTATTCCTTCTTATGTACTTCAACCAGAGTAAAGTTGCAATGATAAAGTTCACAGGATATAAGTTGCTAAACAATATCTCGGACAGTGGGAAGGCTTTTCTTATCATTTTAGTGTCAGATATCCTTCTAGGAACTAGGTTATTTTGCGCCTTAGAAGCTGATTAA
  • >TraesCS7D02G283700.2
    GGTCACCGCAGCGCGGTCTGGGGCTATACAATTCTCTGGCTCCTGGTTGTTGCGATTCGCAGATTCTGCAAAGAAGAGGGGTTGACTGTATTCCGGCCTTCCGGGAGTTCGAAAATTCTTGGTTTCTTGATGTGGATACTGCTACCTTGCAATGACAACGGGTGGCAAGAAGGGAATTCTGTGAGGCTGTGAGCACATGGTTGCCAGAGGTGCGTCCGCCCCCGTTCTGTAGCCCGGAAGATGTGGTCTTCGTTGCATGACCTTTAATGAGTTGCTACGTGGTCAGCTCTAGCGGCGTTGCGGTCTGGTTCGCCGTAGAGGAGAGGATCGGGCACCGGAGGGTTTGCGCATGCAAGATGTTCGATGTCGGTCCCCAGAGGAGGAGGGTGGGGAGGCGCCTGGTGGGTTTTGCCAAGAAGAGGAGGCGTTCCAAGAGGCAGCAGCCATGGTGGAAGGCGTGGTTCTCTGATTGGAACGATGAGGAAGAGAGCCTCGCCGGCTGGAGGGAGGATGATGAATTGCTCCAGCAGGTTGTTAGCAACGAAGACCTGTCGGAGGATGACAAGTTTCAGACGTGGAAGAGCAAGGCCGAGGCGATTGTCGACCTGCGGGAAGCCCAGCAGGATGCCGAAAATGCAGAAGGGCGGTCATGGGAGGATTGGATAGGTTGGGGCAGCACGTCCGGCGATGGTGATTGGGGCGGGGGTGGGAGCTTGTCGGACCAGATAACGGATGATCCGACGGAGATAGTGAGGGACAAGGGCATCGCTGAAGCTTTTAGGGACTCTATTGATGAAGATTACAACGACATGTTGTTTGAGGACCGGGTTTTTCTATACGCTTCGACGAAATCGGCCAAATTCCTAGCATTGTTGATCGTTGTTCCATGGGTGTTGGATCTTCTAGTACATGACTATGTTATGATGCCATTTCTAGACAGGTATGTCGAGAAGGTACCACTCGCCGCTGAAATGCTTGATGTAAGACGCAGCCAGAAGATTCAGATGATAAAGGACCTAAATATTGAGAAAGCAAGATTCCGTTTTGAAGTAGAGATTGGTAAATCTCCTCCACTTTCCGATGAGGAGTTCTGGTCAGAGTTGCGGGAAAAAGCGGTAGAGCTGAGGGATGAATGGAGATTAGAAAACCGACAAGCATTTGCAAATATCTGGTCTGATATGGTTTATGGGGTTGCCCTATTCCTTCTTATGTACTTCAACCAGAGTAAAGTTGCAATGATAAAGTTCACAGGATATAAGTTGCTAAACAATATCTCGGACAGTGGGAAGGCTTTTCTTATCATTTTAGTGTCAGATATCCTTCTAGGAACTAGGTTATTTTGCGCCTTAGAAGCTGATTAA
CDS Sequence
  • >TraesCS7D02G283700.1
    ATGAGTTGCTACGTGGTCAGCTCTAGCGGCGTTGCGGTCTGGTTCGCCGTAGAGGAGAGGATCGGGCACCGGAGGGTTTGCGCATGCAAGATGTTCGATGTCGGTCCCCAGAGGAGGAGGGTGGGGAGGCGCCTGGTGGGTTTTGCCAAGAAGAGGAGGCGTTCCAAGAGGCAGCAGCCATGGTGGAAGGCGTGGTTCTCTGATTGGAACGATGAGGAAGAGAGCCTCGCCGGCTGGAGGGAGGATGATGAATTGCTCCAGCAGGTTGTTAGCAACGAAGACCTGTCGGAGGATGACAAGTTTCAGACGTGGAAGAGCAAGGCCGAGGCGATTGTCGACCTGCGGGAAGCCCAGCAGGATGCCGAAAATGCAGAAGGGCGGTCATGGGAGGATTGGATAGGTTGGGGCAGCACGTCCGGCGATGGTGATTGGGGCGGGGGTGGGAGCTTGTCGGACCAGATAACGGATGATCCGACGGAGATAGTGAGGGACAAGGGCATCGCTGAAGCTTTTAGGGACTCTATTGATGAAGATTACAACGACATGTTGTTTGAGGACCGGGTTTTTCTATACGCTTCGACGAAATCGGCCAAATTCCTAGCATTGTTGATCGTTGTTCCATGGGTGTTGGATCTTCTAGTACATGACTATGTTATGATGCCATTTCTAGACAGGTATGTCGAGAAGGTACCACTCGCCGCTGAAATGCTTGATGTAAGACGCAGCCAGAAGATTCAGATGATAAAGGACCTAAATATTGAGAAAGCAAGATTCCGTTTTGAAGTAGAGATTGGTAAATCTCCTCCACTTTCCGATGAGGAGTTCTGGTCAGAGTTGCGGGAAAAAGCGGTAGAGCTGAGGGATGAATGGAGATTAGAAAACCGACAAGCATTTGCAAATATCTGGTCTGATATGGTTTATGGGGTTGCCCTATTCCTTCTTATGTACTTCAACCAGAGTAAAGTTGCAATGATAAAGTTCACAGGATATAAGTTGCTAAACAATATCTCGGACAGTGGGAAGGCTTTTCTTATCATTTTAGTGTCAGATATCCTTCTAGGAACTAGGTTATTTTGCGCCTTAGAAGCTGATTAA
  • >TraesCS7D02G283700.2
    ATGAGTTGCTACGTGGTCAGCTCTAGCGGCGTTGCGGTCTGGTTCGCCGTAGAGGAGAGGATCGGGCACCGGAGGGTTTGCGCATGCAAGATGTTCGATGTCGGTCCCCAGAGGAGGAGGGTGGGGAGGCGCCTGGTGGGTTTTGCCAAGAAGAGGAGGCGTTCCAAGAGGCAGCAGCCATGGTGGAAGGCGTGGTTCTCTGATTGGAACGATGAGGAAGAGAGCCTCGCCGGCTGGAGGGAGGATGATGAATTGCTCCAGCAGGTTGTTAGCAACGAAGACCTGTCGGAGGATGACAAGTTTCAGACGTGGAAGAGCAAGGCCGAGGCGATTGTCGACCTGCGGGAAGCCCAGCAGGATGCCGAAAATGCAGAAGGGCGGTCATGGGAGGATTGGATAGGTTGGGGCAGCACGTCCGGCGATGGTGATTGGGGCGGGGGTGGGAGCTTGTCGGACCAGATAACGGATGATCCGACGGAGATAGTGAGGGACAAGGGCATCGCTGAAGCTTTTAGGGACTCTATTGATGAAGATTACAACGACATGTTGTTTGAGGACCGGGTTTTTCTATACGCTTCGACGAAATCGGCCAAATTCCTAGCATTGTTGATCGTTGTTCCATGGGTGTTGGATCTTCTAGTACATGACTATGTTATGATGCCATTTCTAGACAGGTATGTCGAGAAGGTACCACTCGCCGCTGAAATGCTTGATGTAAGACGCAGCCAGAAGATTCAGATGATAAAGGACCTAAATATTGAGAAAGCAAGATTCCGTTTTGAAGTAGAGATTGGTAAATCTCCTCCACTTTCCGATGAGGAGTTCTGGTCAGAGTTGCGGGAAAAAGCGGTAGAGCTGAGGGATGAATGGAGATTAGAAAACCGACAAGCATTTGCAAATATCTGGTCTGATATGGTTTATGGGGTTGCCCTATTCCTTCTTATGTACTTCAACCAGAGTAAAGTTGCAATGATAAAGTTCACAGGATATAAGTTGCTAAACAATATCTCGGACAGTGGGAAGGCTTTTCTTATCATTTTAGTGTCAGATATCCTTCTAGGAACTAGGTTATTTTGCGCCTTAGAAGCTGATTAA
Protein Sequence
  • >TraesCS7D02G283700.1
    MSCYVVSSSGVAVWFAVEERIGHRRVCACKMFDVGPQRRRVGRRLVGFAKKRRRSKRQQPWWKAWFSDWNDEEESLAGWREDDELLQQVVSNEDLSEDDKFQTWKSKAEAIVDLREAQQDAENAEGRSWEDWIGWGSTSGDGDWGGGGSLSDQITDDPTEIVRDKGIAEAFRDSIDEDYNDMLFEDRVFLYASTKSAKFLALLIVVPWVLDLLVHDYVMMPFLDRYVEKVPLAAEMLDVRRSQKIQMIKDLNIEKARFRFEVEIGKSPPLSDEEFWSELREKAVELRDEWRLENRQAFANIWSDMVYGVALFLLMYFNQSKVAMIKFTGYKLLNNISDSGKAFLIILVSDILLGTRLFCALEAD
  • >TraesCS7D02G283700.2
    MSCYVVSSSGVAVWFAVEERIGHRRVCACKMFDVGPQRRRVGRRLVGFAKKRRRSKRQQPWWKAWFSDWNDEEESLAGWREDDELLQQVVSNEDLSEDDKFQTWKSKAEAIVDLREAQQDAENAEGRSWEDWIGWGSTSGDGDWGGGGSLSDQITDDPTEIVRDKGIAEAFRDSIDEDYNDMLFEDRVFLYASTKSAKFLALLIVVPWVLDLLVHDYVMMPFLDRYVEKVPLAAEMLDVRRSQKIQMIKDLNIEKARFRFEVEIGKSPPLSDEEFWSELREKAVELRDEWRLENRQAFANIWSDMVYGVALFLLMYFNQSKVAMIKFTGYKLLNNISDSGKAFLIILVSDILLGTRLFCALEAD