Gene Details:
- Gene ID: TraesCS7D02G283700
- Gene Symbol: TaARE1-7D
- Gene Name: chloroplast envelope membrane
- Genome: Chinese_Spring1.0
- Species: Triticum aestivum
Functional Descriptions:
- Expression indicated that knock-out of TaARE1 homoeologs could stimulate nitrate transport rather than ammonium transport upon N deprivation.
- Three TaARE1 homoeologs resulted in more active nitrate transport than ammonium transport upon N deprivation, and the different TaARE1 homoeologs have diverged in this function.
- We isolated and characterized three TaARE1 homeologs in an elite Chinese winter wheat cv ZhengMai 7698 and then generated a series of transgene-free mutant lines either with partial or triple null taare1 alleles through CRISPR/Cas9-mediated targeted mutagenesis of TaARE1.
- All transgene-free mutant lines (TaARE1) showed enhanced tolerance to nitrogen starvation, and delayed senescence and increased grain yield in a field experiment.
Function-related keywords:
- nitrogen , senescence , grain , grain-yield , tolerance , yield , nitrate
Literature:
- Increasing yield potential through manipulating of an ARE1 ortholog related to nitrogen use efficiency in wheat by CRISPR/Cas9. DOI: 10.1111/jipb.13151 ; PMID: 34270164
Related News:
Gene Resources:
- NCBI ID: KAF7108894
- UniProt accessions: A0A3B6TJS2
Sequences:
cDNA Sequence
- >TraesCS7D02G283700.1
GGTCACCGCAGCGCGGTCTGGGGCTATACAATTCTCTGGCTCCTGGTTGTTGCGATTCGCAGATTCTGCAAAGAAGAGGGGTTGACTGTATTCCGGCCTTCCGGGAGTTCGAAAATTCTTGGTTTCTTGATGTGGATACTGCTACCTTGCAATGACAACGGGTGGCAAGAAGGGAATTCTGTGAGGCTGTGAGCACATGGTTGCCAGAGGTGCGTCCGCCCCCGTTCTGTAGCCCGGAAGATGTGGTCTTCGTTGCATGACCTTTAATGAGTTGCTACGTGGTCAGCTCTAGCGGCGTTGCGGTCTGGTTCGCCGTAGAGGAGAGGATCGGGCACCGGAGGGTTTGCGCATGCAAGATGTTCGATGTCGGTCCCCAGAGGAGGAGGGTGGGGAGGCGCCTGGTGGGTTTTGCCAAGAAGAGGAGGCGTTCCAAGAGGCAGCAGCCATGGTGGAAGGCGTGGTTCTCTGATTGGAACGATGAGGAAGAGAGCCTCGCCGGCTGGAGGGAGGATGATGAATTGCTCCAGCAGGTTGTTAGCAACGAAGACCTGTCGGAGGATGACAAGTTTCAGACGTGGAAGAGCAAGGCCGAGGCGATTGTCGACCTGCGGGAAGCCCAGCAGGATGCCGAAAATGCAGAAGGGCGGTCATGGGAGGATTGGATAGGTTGGGGCAGCACGTCCGGCGATGGTGATTGGGGCGGGGGTGGGAGCTTGTCGGACCAGATAACGGATGATCCGACGGAGATAGTGAGGGACAAGGGCATCGCTGAAGCTTTTAGGGACTCTATTGATGAAGATTACAACGACATGTTGTTTGAGGACCGGGTTTTTCTATACGCTTCGACGAAATCGGCCAAATTCCTAGCATTGTTGATCGTTGTTCCATGGGTGTTGGATCTTCTAGTACATGACTATGTTATGATGCCATTTCTAGACAGGTATGTCGAGAAGGTACCACTCGCCGCTGAAATGCTTGATGTAAGACGCAGCCAGAAGATTCAGATGATAAAGGACCTAAATATTGAGAAAGCAAGATTCCGTTTTGAAGTAGAGATTGGTAAATCTCCTCCACTTTCCGATGAGGAGTTCTGGTCAGAGTTGCGGGAAAAAGCGGTAGAGCTGAGGGATGAATGGAGATTAGAAAACCGACAAGCATTTGCAAATATCTGGTCTGATATGGTTTATGGGGTTGCCCTATTCCTTCTTATGTACTTCAACCAGAGTAAAGTTGCAATGATAAAGTTCACAGGATATAAGTTGCTAAACAATATCTCGGACAGTGGGAAGGCTTTTCTTATCATTTTAGTGTCAGATATCCTTCTAGGAACTAGGTTATTTTGCGCCTTAGAAGCTGATTAA - >TraesCS7D02G283700.2
GGTCACCGCAGCGCGGTCTGGGGCTATACAATTCTCTGGCTCCTGGTTGTTGCGATTCGCAGATTCTGCAAAGAAGAGGGGTTGACTGTATTCCGGCCTTCCGGGAGTTCGAAAATTCTTGGTTTCTTGATGTGGATACTGCTACCTTGCAATGACAACGGGTGGCAAGAAGGGAATTCTGTGAGGCTGTGAGCACATGGTTGCCAGAGGTGCGTCCGCCCCCGTTCTGTAGCCCGGAAGATGTGGTCTTCGTTGCATGACCTTTAATGAGTTGCTACGTGGTCAGCTCTAGCGGCGTTGCGGTCTGGTTCGCCGTAGAGGAGAGGATCGGGCACCGGAGGGTTTGCGCATGCAAGATGTTCGATGTCGGTCCCCAGAGGAGGAGGGTGGGGAGGCGCCTGGTGGGTTTTGCCAAGAAGAGGAGGCGTTCCAAGAGGCAGCAGCCATGGTGGAAGGCGTGGTTCTCTGATTGGAACGATGAGGAAGAGAGCCTCGCCGGCTGGAGGGAGGATGATGAATTGCTCCAGCAGGTTGTTAGCAACGAAGACCTGTCGGAGGATGACAAGTTTCAGACGTGGAAGAGCAAGGCCGAGGCGATTGTCGACCTGCGGGAAGCCCAGCAGGATGCCGAAAATGCAGAAGGGCGGTCATGGGAGGATTGGATAGGTTGGGGCAGCACGTCCGGCGATGGTGATTGGGGCGGGGGTGGGAGCTTGTCGGACCAGATAACGGATGATCCGACGGAGATAGTGAGGGACAAGGGCATCGCTGAAGCTTTTAGGGACTCTATTGATGAAGATTACAACGACATGTTGTTTGAGGACCGGGTTTTTCTATACGCTTCGACGAAATCGGCCAAATTCCTAGCATTGTTGATCGTTGTTCCATGGGTGTTGGATCTTCTAGTACATGACTATGTTATGATGCCATTTCTAGACAGGTATGTCGAGAAGGTACCACTCGCCGCTGAAATGCTTGATGTAAGACGCAGCCAGAAGATTCAGATGATAAAGGACCTAAATATTGAGAAAGCAAGATTCCGTTTTGAAGTAGAGATTGGTAAATCTCCTCCACTTTCCGATGAGGAGTTCTGGTCAGAGTTGCGGGAAAAAGCGGTAGAGCTGAGGGATGAATGGAGATTAGAAAACCGACAAGCATTTGCAAATATCTGGTCTGATATGGTTTATGGGGTTGCCCTATTCCTTCTTATGTACTTCAACCAGAGTAAAGTTGCAATGATAAAGTTCACAGGATATAAGTTGCTAAACAATATCTCGGACAGTGGGAAGGCTTTTCTTATCATTTTAGTGTCAGATATCCTTCTAGGAACTAGGTTATTTTGCGCCTTAGAAGCTGATTAA
CDS Sequence
- >TraesCS7D02G283700.1
ATGAGTTGCTACGTGGTCAGCTCTAGCGGCGTTGCGGTCTGGTTCGCCGTAGAGGAGAGGATCGGGCACCGGAGGGTTTGCGCATGCAAGATGTTCGATGTCGGTCCCCAGAGGAGGAGGGTGGGGAGGCGCCTGGTGGGTTTTGCCAAGAAGAGGAGGCGTTCCAAGAGGCAGCAGCCATGGTGGAAGGCGTGGTTCTCTGATTGGAACGATGAGGAAGAGAGCCTCGCCGGCTGGAGGGAGGATGATGAATTGCTCCAGCAGGTTGTTAGCAACGAAGACCTGTCGGAGGATGACAAGTTTCAGACGTGGAAGAGCAAGGCCGAGGCGATTGTCGACCTGCGGGAAGCCCAGCAGGATGCCGAAAATGCAGAAGGGCGGTCATGGGAGGATTGGATAGGTTGGGGCAGCACGTCCGGCGATGGTGATTGGGGCGGGGGTGGGAGCTTGTCGGACCAGATAACGGATGATCCGACGGAGATAGTGAGGGACAAGGGCATCGCTGAAGCTTTTAGGGACTCTATTGATGAAGATTACAACGACATGTTGTTTGAGGACCGGGTTTTTCTATACGCTTCGACGAAATCGGCCAAATTCCTAGCATTGTTGATCGTTGTTCCATGGGTGTTGGATCTTCTAGTACATGACTATGTTATGATGCCATTTCTAGACAGGTATGTCGAGAAGGTACCACTCGCCGCTGAAATGCTTGATGTAAGACGCAGCCAGAAGATTCAGATGATAAAGGACCTAAATATTGAGAAAGCAAGATTCCGTTTTGAAGTAGAGATTGGTAAATCTCCTCCACTTTCCGATGAGGAGTTCTGGTCAGAGTTGCGGGAAAAAGCGGTAGAGCTGAGGGATGAATGGAGATTAGAAAACCGACAAGCATTTGCAAATATCTGGTCTGATATGGTTTATGGGGTTGCCCTATTCCTTCTTATGTACTTCAACCAGAGTAAAGTTGCAATGATAAAGTTCACAGGATATAAGTTGCTAAACAATATCTCGGACAGTGGGAAGGCTTTTCTTATCATTTTAGTGTCAGATATCCTTCTAGGAACTAGGTTATTTTGCGCCTTAGAAGCTGATTAA - >TraesCS7D02G283700.2
ATGAGTTGCTACGTGGTCAGCTCTAGCGGCGTTGCGGTCTGGTTCGCCGTAGAGGAGAGGATCGGGCACCGGAGGGTTTGCGCATGCAAGATGTTCGATGTCGGTCCCCAGAGGAGGAGGGTGGGGAGGCGCCTGGTGGGTTTTGCCAAGAAGAGGAGGCGTTCCAAGAGGCAGCAGCCATGGTGGAAGGCGTGGTTCTCTGATTGGAACGATGAGGAAGAGAGCCTCGCCGGCTGGAGGGAGGATGATGAATTGCTCCAGCAGGTTGTTAGCAACGAAGACCTGTCGGAGGATGACAAGTTTCAGACGTGGAAGAGCAAGGCCGAGGCGATTGTCGACCTGCGGGAAGCCCAGCAGGATGCCGAAAATGCAGAAGGGCGGTCATGGGAGGATTGGATAGGTTGGGGCAGCACGTCCGGCGATGGTGATTGGGGCGGGGGTGGGAGCTTGTCGGACCAGATAACGGATGATCCGACGGAGATAGTGAGGGACAAGGGCATCGCTGAAGCTTTTAGGGACTCTATTGATGAAGATTACAACGACATGTTGTTTGAGGACCGGGTTTTTCTATACGCTTCGACGAAATCGGCCAAATTCCTAGCATTGTTGATCGTTGTTCCATGGGTGTTGGATCTTCTAGTACATGACTATGTTATGATGCCATTTCTAGACAGGTATGTCGAGAAGGTACCACTCGCCGCTGAAATGCTTGATGTAAGACGCAGCCAGAAGATTCAGATGATAAAGGACCTAAATATTGAGAAAGCAAGATTCCGTTTTGAAGTAGAGATTGGTAAATCTCCTCCACTTTCCGATGAGGAGTTCTGGTCAGAGTTGCGGGAAAAAGCGGTAGAGCTGAGGGATGAATGGAGATTAGAAAACCGACAAGCATTTGCAAATATCTGGTCTGATATGGTTTATGGGGTTGCCCTATTCCTTCTTATGTACTTCAACCAGAGTAAAGTTGCAATGATAAAGTTCACAGGATATAAGTTGCTAAACAATATCTCGGACAGTGGGAAGGCTTTTCTTATCATTTTAGTGTCAGATATCCTTCTAGGAACTAGGTTATTTTGCGCCTTAGAAGCTGATTAA
Protein Sequence
- >TraesCS7D02G283700.1
MSCYVVSSSGVAVWFAVEERIGHRRVCACKMFDVGPQRRRVGRRLVGFAKKRRRSKRQQPWWKAWFSDWNDEEESLAGWREDDELLQQVVSNEDLSEDDKFQTWKSKAEAIVDLREAQQDAENAEGRSWEDWIGWGSTSGDGDWGGGGSLSDQITDDPTEIVRDKGIAEAFRDSIDEDYNDMLFEDRVFLYASTKSAKFLALLIVVPWVLDLLVHDYVMMPFLDRYVEKVPLAAEMLDVRRSQKIQMIKDLNIEKARFRFEVEIGKSPPLSDEEFWSELREKAVELRDEWRLENRQAFANIWSDMVYGVALFLLMYFNQSKVAMIKFTGYKLLNNISDSGKAFLIILVSDILLGTRLFCALEAD - >TraesCS7D02G283700.2
MSCYVVSSSGVAVWFAVEERIGHRRVCACKMFDVGPQRRRVGRRLVGFAKKRRRSKRQQPWWKAWFSDWNDEEESLAGWREDDELLQQVVSNEDLSEDDKFQTWKSKAEAIVDLREAQQDAENAEGRSWEDWIGWGSTSGDGDWGGGGSLSDQITDDPTEIVRDKGIAEAFRDSIDEDYNDMLFEDRVFLYASTKSAKFLALLIVVPWVLDLLVHDYVMMPFLDRYVEKVPLAAEMLDVRRSQKIQMIKDLNIEKARFRFEVEIGKSPPLSDEEFWSELREKAVELRDEWRLENRQAFANIWSDMVYGVALFLLMYFNQSKVAMIKFTGYKLLNNISDSGKAFLIILVSDILLGTRLFCALEAD