Information report for TaAQP8
Gene Details
|
|
Functional Descriptions
- Overexpression of a Wheat Aquaporin Gene, TaAQP8, Enhances Salt Stress Tolerance in Transgenic Tobacco.
- Transient expression of TaAQP8–green fluorescent protein (GFP) fusion protein revealed its localization in the plasma membrane. TaAQP8 exhibited water channel activity in Xenopus laevis oocytes.
- Investigation showed that up-regulation of TaAQP8 under salt stress involves ethylene and H2O2 signaling, with ethylene causing a positive effect and H2O2 acting as a negative factor.
- Overexpression of TaAQP8 in tobacco increased root elongation compared with controls under salt stress. The roots of transgenic plants also retained a high K+/Na+ ratio and Ca2+ content, but reduced H2O2 accumulation by an enhancement of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities under salt stress.
- Our results demonstrate that TaAQP8 confers salt stress tolerance not only by retaining high a K+/Na+ ratio and Ca2+ content, but also by reducing H2O2 accumulation and membrane damage by enhancing the antioxidant system.
Functional Keywords
- root , stress , salt , tolerance , ethylene , root-elongation , salt-tolerance , salt-stress , stress-tolerance , plasma-membrane , catalase , aquaporin-water-channel , ethylene-content , h2o2 , protein
Literature and News
Gene Resources
- NCBI ID: HQ650110.1
- UniProt accessions: G3E8E2
- EnsemblPlants: TraesCS2A02G407700.1
- Gramene: TraesCS2A02G407700.1
- GO: GO:0015267 , GO:0016020
- OMA: IFNNDHA
- ExpressionAtlas: TraesCS2A02G407700
- InterPro: IPR000425 , IPR022357 , IPR023271 , IPR034294
- PANTHER: PTHR45687
- SUPFAM: SSF81338
- PROSITE: PS00221
- Gene3D: 1.20.1080.10
- OrthoDB: G3E8E2
- SMR: G3E8E2
- CDD: cd00333
Sequences
cDNA Sequence
- >TraesCS2A02G407700.1
GTTCCAGCTCAGGCGAGCAGCGACCACCTTCCGGCGAGGGCAGCAGCGATGGAGGGCAAGGAGGAGGACGTGCGGCTCGGGGCGAACAAGTACTCGGAGCGGCAGCCCATCGGCACGGCGGCGCAGGGGTCCGAGGACAAGGACTACAAGGAGCCCCCGCCGGCGCCGCTGTTCGAGCCCGGCGAGCTCAAGTCCTGGTCCTTCTACCGCGCCGGCATCGCCGAGTTCATGGCCACCTTCCTCTTCCTCTACGTCACCATCCTCACCGTGATGGGCTACAGCGGCGCCACCTCCAAGTGCGCCAGCGTCGGCATCCAGGGCATCGCCTGGTCCTTCGGCGGCATGATCTTCGCCCTCGTCTACTGCACCGCCGGCATCTCCGGCGGGCACATCAACCCGGCGGTGACCTTCGGGCTGTTCCTGGCGAGGAAGCTGTCGCTGACCCGGGCGGTGTTCTACATCATCATGCAGTGCCTGGGCGCCATCTGCGGCGCCGGCGTGGTGAAGGGGTTCCAGCAGGGCCTGTACATGGGCAACGGCGGCGGCGCCAACGTGGTGGCGCCCGGCTACACCAAGGGCTCCGGCCTCGGCGCCGAGATCATCGGCACCTTCGTCCTCGTCTACACCGTCTTCTCCGCCACCGACGCCAAGAGGAACGCCAGGGACTCCCACGTTCCCATCCTCGCCCCGCTGCCCATCGGGTTCGCCGTGTTCCTGGTGCACCTGGCCACCATCCCCATCACCGGCACCGGCATCAACCCGGCGAGGAGCCTCGGCGCGGCCATCATCTACAACAGGGAGCACGCCTGGTCAGACCACTGGATCTTCTGGGTCGGCCCCTTCATCGGCGCCGCGCTGGCTGCCGTCTACCACCAGGTGGTCATCAGAGCGATCCCGTTCAAGACCAAGTCCTAAGCAGCTGTTCTTCCTGCTACAAAGAAGATGCCAACCCAACCCGAAGAGCAAGCTGCGTGTTCTGAATTTCTGATGGGGCGGCTATATATCTCTTCCCGTCATCCGTGTTACTGTGGAATTCCAATTTGTCTTCCAAGTTTGATGTACTCTGTATTCACCTGAACCTGGACCCAGACTTGATCTGGTTCAAACCCTACCGCGTCTGCGGCGGGGGTGACGGCATAGACGGCAGGCGTTTAGCTGCACAGCCGTGGATACAATTCCTAGCGTCAGGGTCCCTAACGGTAGGTTGTTACACTCTACCGCCATCGACCCCGACGGTAGGAAAAGGGGTTAGATCCCCAAATTTTATCA - >TraesCS2A02G407700.2
GTTCCAGCTCAGGCGAGCAGCGACCACCTTCCGGCGAGGGCAGCAGCGATGGAGGGCAAGGAGGAGGACGTGCGGCTCGGGGCGAACAAGTACTCGGAGCGGCAGCCCATCGGCACGGCGGCGCAGGGGTCCGAGGACAAGGACTACAAGGAGCCCCCGCCGGCGCCGCTGTTCGAGCCCGGCGAGCTCAAGTCCTGGTCCTTCTACCGCGCCGGCATCGCCGAGTTCATGGCCACCTTCCTCTTCCTCTACGTCACCATCCTCACCGTGATGGGCTACAGCGGCGCCACCTCCAAGTGCGCCAGCGTCGGCATCCAGGGCATCGCCTGGTCCTTCGGCGGCATGATCTTCGCCCTCGTCTACTGCACCGCCGGCATCTCCGGCGGGCACATCAACCCGGCGGTGACCTTCGGGCTGTTCCTGGCGAGGAAGCTGTCGCTGACCCGGGCGGTGTTCTACATCATCATGCAGTGCCTGGGCGCCATCTGCGGCGCCGGCGTGGTGAAGGGGTTCCAGCAGGGCCTGTACATGGGCAACGGCGGCGGCGCCAACGTGGTGGCGCCCGGCTACACCAAGGGCTCCGGCCTCGGCGCCGAGATCATCGGCACCTTCGTCCTCGTCTACACCGTCTTCTCCGCCACCGACGCCAAGAGGAACGCCAGGGACTCCCACGTTCCCATCCTCGCCCCGCTGCCCATCGGGTTCGCCGTGTTCCTGGTGCACCTGGCCACCATCCCCATCACCGGCACCGGCATCAACCCGGCGAGGAGCCTCGGCGCGGCCATCATCTACAACAGGGAGCACGCCTGGTCAGACCACTGGATCTTCTGGGTCGGCCCCTTCATCGGCGCCGCGCTGGCTGCCGTCTACCACCAGGTGGTCATCAGAGCGATCCCGTTCAAGACCAAGTCCTAAGCAGCTGTTCTTCCTGCTACAAAGAAGATGCCAACCCAACCCGAAGAGCAAGCTGCGTGTTCTGAATTTCTGATGGGGCGGCTATATATCTCTTCCCGTCATCCGTGTTACTGTGGAATTCCAATTTGTCTTCCAAGTTTGATGTACTCTGTATTCACCTGAACCTGGACCCAGACTTGATCTGGTTCAAACCCTACCGCGTCTGCGGCGGGGGTGACGGCATAGACGGCAGGCGTTTAGCTGCACAGCCGTGGATACAATTCCTAGCGTCAGGGTCCCTAACGGTAGGTTGTTACACTCTACCGCCATCGACCCCGACGGTAGGAAAAGGGGTTAGATCCCCAAATTTTATCA
CDS Sequence
- >TraesCS2A02G407700.1
ATGGAGGGCAAGGAGGAGGACGTGCGGCTCGGGGCGAACAAGTACTCGGAGCGGCAGCCCATCGGCACGGCGGCGCAGGGGTCCGAGGACAAGGACTACAAGGAGCCCCCGCCGGCGCCGCTGTTCGAGCCCGGCGAGCTCAAGTCCTGGTCCTTCTACCGCGCCGGCATCGCCGAGTTCATGGCCACCTTCCTCTTCCTCTACGTCACCATCCTCACCGTGATGGGCTACAGCGGCGCCACCTCCAAGTGCGCCAGCGTCGGCATCCAGGGCATCGCCTGGTCCTTCGGCGGCATGATCTTCGCCCTCGTCTACTGCACCGCCGGCATCTCCGGCGGGCACATCAACCCGGCGGTGACCTTCGGGCTGTTCCTGGCGAGGAAGCTGTCGCTGACCCGGGCGGTGTTCTACATCATCATGCAGTGCCTGGGCGCCATCTGCGGCGCCGGCGTGGTGAAGGGGTTCCAGCAGGGCCTGTACATGGGCAACGGCGGCGGCGCCAACGTGGTGGCGCCCGGCTACACCAAGGGCTCCGGCCTCGGCGCCGAGATCATCGGCACCTTCGTCCTCGTCTACACCGTCTTCTCCGCCACCGACGCCAAGAGGAACGCCAGGGACTCCCACGTTCCCATCCTCGCCCCGCTGCCCATCGGGTTCGCCGTGTTCCTGGTGCACCTGGCCACCATCCCCATCACCGGCACCGGCATCAACCCGGCGAGGAGCCTCGGCGCGGCCATCATCTACAACAGGGAGCACGCCTGGTCAGACCACTGGATCTTCTGGGTCGGCCCCTTCATCGGCGCCGCGCTGGCTGCCGTCTACCACCAGGTGGTCATCAGAGCGATCCCGTTCAAGACCAAGTCCTAA - >TraesCS2A02G407700.2
ATGGAGGGCAAGGAGGAGGACGTGCGGCTCGGGGCGAACAAGTACTCGGAGCGGCAGCCCATCGGCACGGCGGCGCAGGGGTCCGAGGACAAGGACTACAAGGAGCCCCCGCCGGCGCCGCTGTTCGAGCCCGGCGAGCTCAAGTCCTGGTCCTTCTACCGCGCCGGCATCGCCGAGTTCATGGCCACCTTCCTCTTCCTCTACGTCACCATCCTCACCGTGATGGGCTACAGCGGCGCCACCTCCAAGTGCGCCAGCGTCGGCATCCAGGGCATCGCCTGGTCCTTCGGCGGCATGATCTTCGCCCTCGTCTACTGCACCGCCGGCATCTCCGGCGGGCACATCAACCCGGCGGTGACCTTCGGGCTGTTCCTGGCGAGGAAGCTGTCGCTGACCCGGGCGGTGTTCTACATCATCATGCAGTGCCTGGGCGCCATCTGCGGCGCCGGCGTGGTGAAGGGGTTCCAGCAGGGCCTGTACATGGGCAACGGCGGCGGCGCCAACGTGGTGGCGCCCGGCTACACCAAGGGCTCCGGCCTCGGCGCCGAGATCATCGGCACCTTCGTCCTCGTCTACACCGTCTTCTCCGCCACCGACGCCAAGAGGAACGCCAGGGACTCCCACGTTCCCATCCTCGCCCCGCTGCCCATCGGGTTCGCCGTGTTCCTGGTGCACCTGGCCACCATCCCCATCACCGGCACCGGCATCAACCCGGCGAGGAGCCTCGGCGCGGCCATCATCTACAACAGGGAGCACGCCTGGTCAGACCACTGGATCTTCTGGGTCGGCCCCTTCATCGGCGCCGCGCTGGCTGCCGTCTACCACCAGGTGGTCATCAGAGCGATCCCGTTCAAGACCAAGTCCTAA
Protein Sequence
- >TraesCS2A02G407700.1
MEGKEEDVRLGANKYSERQPIGTAAQGSEDKDYKEPPPAPLFEPGELKSWSFYRAGIAEFMATFLFLYVTILTVMGYSGATSKCASVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAVFYIIMQCLGAICGAGVVKGFQQGLYMGNGGGANVVAPGYTKGSGLGAEIIGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIYNREHAWSDHWIFWVGPFIGAALAAVYHQVVIRAIPFKTKS - >TraesCS2A02G407700.2
MEGKEEDVRLGANKYSERQPIGTAAQGSEDKDYKEPPPAPLFEPGELKSWSFYRAGIAEFMATFLFLYVTILTVMGYSGATSKCASVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAVFYIIMQCLGAICGAGVVKGFQQGLYMGNGGGANVVAPGYTKGSGLGAEIIGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIYNREHAWSDHWIFWVGPFIGAALAAVYHQVVIRAIPFKTKS