Gene Details:

Functional Descriptions:

  • Overexpression of a Wheat Aquaporin Gene, TaAQP8, Enhances Salt Stress Tolerance in Transgenic Tobacco.
  • Transient expression of TaAQP8–green fluorescent protein (GFP) fusion protein revealed its localization in the plasma membrane. TaAQP8 exhibited water channel activity in Xenopus laevis oocytes.
  • Investigation showed that up-regulation of TaAQP8 under salt stress involves ethylene and H2O2 signaling, with ethylene causing a positive effect and H2O2 acting as a negative factor.
  • Overexpression of TaAQP8 in tobacco increased root elongation compared with controls under salt stress. The roots of transgenic plants also retained a high K+/Na+ ratio and Ca2+ content, but reduced H2O2 accumulation by an enhancement of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities under salt stress.
  • Our results demonstrate that TaAQP8 confers salt stress tolerance not only by retaining high a K+/Na+ ratio and Ca2+ content, but also by reducing H2O2 accumulation and membrane damage by enhancing the antioxidant system.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >TraesCS2A02G407700.1
    GTTCCAGCTCAGGCGAGCAGCGACCACCTTCCGGCGAGGGCAGCAGCGATGGAGGGCAAGGAGGAGGACGTGCGGCTCGGGGCGAACAAGTACTCGGAGCGGCAGCCCATCGGCACGGCGGCGCAGGGGTCCGAGGACAAGGACTACAAGGAGCCCCCGCCGGCGCCGCTGTTCGAGCCCGGCGAGCTCAAGTCCTGGTCCTTCTACCGCGCCGGCATCGCCGAGTTCATGGCCACCTTCCTCTTCCTCTACGTCACCATCCTCACCGTGATGGGCTACAGCGGCGCCACCTCCAAGTGCGCCAGCGTCGGCATCCAGGGCATCGCCTGGTCCTTCGGCGGCATGATCTTCGCCCTCGTCTACTGCACCGCCGGCATCTCCGGCGGGCACATCAACCCGGCGGTGACCTTCGGGCTGTTCCTGGCGAGGAAGCTGTCGCTGACCCGGGCGGTGTTCTACATCATCATGCAGTGCCTGGGCGCCATCTGCGGCGCCGGCGTGGTGAAGGGGTTCCAGCAGGGCCTGTACATGGGCAACGGCGGCGGCGCCAACGTGGTGGCGCCCGGCTACACCAAGGGCTCCGGCCTCGGCGCCGAGATCATCGGCACCTTCGTCCTCGTCTACACCGTCTTCTCCGCCACCGACGCCAAGAGGAACGCCAGGGACTCCCACGTTCCCATCCTCGCCCCGCTGCCCATCGGGTTCGCCGTGTTCCTGGTGCACCTGGCCACCATCCCCATCACCGGCACCGGCATCAACCCGGCGAGGAGCCTCGGCGCGGCCATCATCTACAACAGGGAGCACGCCTGGTCAGACCACTGGATCTTCTGGGTCGGCCCCTTCATCGGCGCCGCGCTGGCTGCCGTCTACCACCAGGTGGTCATCAGAGCGATCCCGTTCAAGACCAAGTCCTAAGCAGCTGTTCTTCCTGCTACAAAGAAGATGCCAACCCAACCCGAAGAGCAAGCTGCGTGTTCTGAATTTCTGATGGGGCGGCTATATATCTCTTCCCGTCATCCGTGTTACTGTGGAATTCCAATTTGTCTTCCAAGTTTGATGTACTCTGTATTCACCTGAACCTGGACCCAGACTTGATCTGGTTCAAACCCTACCGCGTCTGCGGCGGGGGTGACGGCATAGACGGCAGGCGTTTAGCTGCACAGCCGTGGATACAATTCCTAGCGTCAGGGTCCCTAACGGTAGGTTGTTACACTCTACCGCCATCGACCCCGACGGTAGGAAAAGGGGTTAGATCCCCAAATTTTATCA
  • >TraesCS2A02G407700.2
    GTTCCAGCTCAGGCGAGCAGCGACCACCTTCCGGCGAGGGCAGCAGCGATGGAGGGCAAGGAGGAGGACGTGCGGCTCGGGGCGAACAAGTACTCGGAGCGGCAGCCCATCGGCACGGCGGCGCAGGGGTCCGAGGACAAGGACTACAAGGAGCCCCCGCCGGCGCCGCTGTTCGAGCCCGGCGAGCTCAAGTCCTGGTCCTTCTACCGCGCCGGCATCGCCGAGTTCATGGCCACCTTCCTCTTCCTCTACGTCACCATCCTCACCGTGATGGGCTACAGCGGCGCCACCTCCAAGTGCGCCAGCGTCGGCATCCAGGGCATCGCCTGGTCCTTCGGCGGCATGATCTTCGCCCTCGTCTACTGCACCGCCGGCATCTCCGGCGGGCACATCAACCCGGCGGTGACCTTCGGGCTGTTCCTGGCGAGGAAGCTGTCGCTGACCCGGGCGGTGTTCTACATCATCATGCAGTGCCTGGGCGCCATCTGCGGCGCCGGCGTGGTGAAGGGGTTCCAGCAGGGCCTGTACATGGGCAACGGCGGCGGCGCCAACGTGGTGGCGCCCGGCTACACCAAGGGCTCCGGCCTCGGCGCCGAGATCATCGGCACCTTCGTCCTCGTCTACACCGTCTTCTCCGCCACCGACGCCAAGAGGAACGCCAGGGACTCCCACGTTCCCATCCTCGCCCCGCTGCCCATCGGGTTCGCCGTGTTCCTGGTGCACCTGGCCACCATCCCCATCACCGGCACCGGCATCAACCCGGCGAGGAGCCTCGGCGCGGCCATCATCTACAACAGGGAGCACGCCTGGTCAGACCACTGGATCTTCTGGGTCGGCCCCTTCATCGGCGCCGCGCTGGCTGCCGTCTACCACCAGGTGGTCATCAGAGCGATCCCGTTCAAGACCAAGTCCTAAGCAGCTGTTCTTCCTGCTACAAAGAAGATGCCAACCCAACCCGAAGAGCAAGCTGCGTGTTCTGAATTTCTGATGGGGCGGCTATATATCTCTTCCCGTCATCCGTGTTACTGTGGAATTCCAATTTGTCTTCCAAGTTTGATGTACTCTGTATTCACCTGAACCTGGACCCAGACTTGATCTGGTTCAAACCCTACCGCGTCTGCGGCGGGGGTGACGGCATAGACGGCAGGCGTTTAGCTGCACAGCCGTGGATACAATTCCTAGCGTCAGGGTCCCTAACGGTAGGTTGTTACACTCTACCGCCATCGACCCCGACGGTAGGAAAAGGGGTTAGATCCCCAAATTTTATCA
CDS Sequence
  • >TraesCS2A02G407700.1
    ATGGAGGGCAAGGAGGAGGACGTGCGGCTCGGGGCGAACAAGTACTCGGAGCGGCAGCCCATCGGCACGGCGGCGCAGGGGTCCGAGGACAAGGACTACAAGGAGCCCCCGCCGGCGCCGCTGTTCGAGCCCGGCGAGCTCAAGTCCTGGTCCTTCTACCGCGCCGGCATCGCCGAGTTCATGGCCACCTTCCTCTTCCTCTACGTCACCATCCTCACCGTGATGGGCTACAGCGGCGCCACCTCCAAGTGCGCCAGCGTCGGCATCCAGGGCATCGCCTGGTCCTTCGGCGGCATGATCTTCGCCCTCGTCTACTGCACCGCCGGCATCTCCGGCGGGCACATCAACCCGGCGGTGACCTTCGGGCTGTTCCTGGCGAGGAAGCTGTCGCTGACCCGGGCGGTGTTCTACATCATCATGCAGTGCCTGGGCGCCATCTGCGGCGCCGGCGTGGTGAAGGGGTTCCAGCAGGGCCTGTACATGGGCAACGGCGGCGGCGCCAACGTGGTGGCGCCCGGCTACACCAAGGGCTCCGGCCTCGGCGCCGAGATCATCGGCACCTTCGTCCTCGTCTACACCGTCTTCTCCGCCACCGACGCCAAGAGGAACGCCAGGGACTCCCACGTTCCCATCCTCGCCCCGCTGCCCATCGGGTTCGCCGTGTTCCTGGTGCACCTGGCCACCATCCCCATCACCGGCACCGGCATCAACCCGGCGAGGAGCCTCGGCGCGGCCATCATCTACAACAGGGAGCACGCCTGGTCAGACCACTGGATCTTCTGGGTCGGCCCCTTCATCGGCGCCGCGCTGGCTGCCGTCTACCACCAGGTGGTCATCAGAGCGATCCCGTTCAAGACCAAGTCCTAA
  • >TraesCS2A02G407700.2
    ATGGAGGGCAAGGAGGAGGACGTGCGGCTCGGGGCGAACAAGTACTCGGAGCGGCAGCCCATCGGCACGGCGGCGCAGGGGTCCGAGGACAAGGACTACAAGGAGCCCCCGCCGGCGCCGCTGTTCGAGCCCGGCGAGCTCAAGTCCTGGTCCTTCTACCGCGCCGGCATCGCCGAGTTCATGGCCACCTTCCTCTTCCTCTACGTCACCATCCTCACCGTGATGGGCTACAGCGGCGCCACCTCCAAGTGCGCCAGCGTCGGCATCCAGGGCATCGCCTGGTCCTTCGGCGGCATGATCTTCGCCCTCGTCTACTGCACCGCCGGCATCTCCGGCGGGCACATCAACCCGGCGGTGACCTTCGGGCTGTTCCTGGCGAGGAAGCTGTCGCTGACCCGGGCGGTGTTCTACATCATCATGCAGTGCCTGGGCGCCATCTGCGGCGCCGGCGTGGTGAAGGGGTTCCAGCAGGGCCTGTACATGGGCAACGGCGGCGGCGCCAACGTGGTGGCGCCCGGCTACACCAAGGGCTCCGGCCTCGGCGCCGAGATCATCGGCACCTTCGTCCTCGTCTACACCGTCTTCTCCGCCACCGACGCCAAGAGGAACGCCAGGGACTCCCACGTTCCCATCCTCGCCCCGCTGCCCATCGGGTTCGCCGTGTTCCTGGTGCACCTGGCCACCATCCCCATCACCGGCACCGGCATCAACCCGGCGAGGAGCCTCGGCGCGGCCATCATCTACAACAGGGAGCACGCCTGGTCAGACCACTGGATCTTCTGGGTCGGCCCCTTCATCGGCGCCGCGCTGGCTGCCGTCTACCACCAGGTGGTCATCAGAGCGATCCCGTTCAAGACCAAGTCCTAA
Protein Sequence
  • >TraesCS2A02G407700.1
    MEGKEEDVRLGANKYSERQPIGTAAQGSEDKDYKEPPPAPLFEPGELKSWSFYRAGIAEFMATFLFLYVTILTVMGYSGATSKCASVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAVFYIIMQCLGAICGAGVVKGFQQGLYMGNGGGANVVAPGYTKGSGLGAEIIGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIYNREHAWSDHWIFWVGPFIGAALAAVYHQVVIRAIPFKTKS
  • >TraesCS2A02G407700.2
    MEGKEEDVRLGANKYSERQPIGTAAQGSEDKDYKEPPPAPLFEPGELKSWSFYRAGIAEFMATFLFLYVTILTVMGYSGATSKCASVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAVFYIIMQCLGAICGAGVVKGFQQGLYMGNGGGANVVAPGYTKGSGLGAEIIGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIYNREHAWSDHWIFWVGPFIGAALAAVYHQVVIRAIPFKTKS