Gene Details:

Functional Descriptions:

  • Designated ORF1 as RNHL-D1 and ORF2 as RNHL-D1.2. Two independent rnhl-d1 mutants showed significant reduction in plant height and spike length compared to the non-edited control. The RNHL-D1-overexpressing transgenic lines showed significantly increased plant height and SL.
  • Suggesting a significant dosage effect of RNHL1 homoeologs in regulating plant height.
  • Our phenotyping and genetic mapping analyses collectively suggested that these two newly identified EMS-induced mutants are allelic to Rht8, thus we renamed them Rht8-2 and Rht8-3,respectively. Rht8-2 and Rht8-3 reduced plant height and had no obvious negative impact on grain yield.
  • A natural variation in Ribonuclease H-like gene underlies Rht8 to confer “Green Revolution” trait in wheat.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >TraesCS2B02G073600.1
    ACGGACGTCTCTGTTTGTTTTTGGCCTTCTCTTCTTCTTCTCGGAGGAGAGAGCGGGCCTCAGGACACCGGGGGATATACTAGTCGGGCTACACGGGGCCCGAGATAAATCGGGGGGAGAAAAAAAGGGGGCGGGGGTGAAAAATACGCCGCACCACTTCTCTCTTGACGCGGCCCTCCGCTCGGGCGATAGTTGGAAAGGAGAGCGAAACGGGCGCCCGAAACCCCCTGAAAACCTCCGCCTGACGCGCCCGCCCATGTCGGCGCCGGAGGAGGGCGACGCCGCCGCCTCAGGACCGTCGGCGCCGCCGCCGCCTCCCGCGCCCGCGCCGATGGCGATGGCGGAGCCCGGCGCAATGGGGCCCGAGGAGGCCGCCGCCAGGAAGCGCTACGAGGCGCTGATGCAGGTGCGGGCCAAGGCGCTCAAGGGCAAGGGCGCCTGGTACTGGGGCCACCTCGAGCCCGTCCTCGTCCCGCCGCCGGCCTCCGGGGCGCCCCCCAAGGCCGCCCGCCTCCGATGCGCGCTCTGCGCCGCCACCTTCTCCGCCTCCAACCCCTCCCGCACCGCCACCGAGCACCTCAAGCGCGGCGCCTGCCCTAATTTCGCGGCGGCGCAGGGCGCGCCGCCGCCGCCGCCGCCGCCGCCCCCGCCCCCGCCGCAGAATCAGCATCAGCAGTTGCAGCTCGTCGCCGTCTCGTCCCCCGCCTCATCGATCGTGCCCATCTCCTCCATCCCCCCGTCCTCCTCCTCCTCCTCGCAGCGCCGCCACTCCACCGGCGTTGGCGGCCGGAAGCGCCACGCGCTCGCCGCGGCCTACGCATCCGTCGAGGCGGCCGCGCACCAGCACCACGTCATGGTCGCCGACCCGGCCGGGTACTCCCCGACGCCGCCCACCCCGCCCGGGATGCCCGCGCCCAGGCAGCTGCTCTCCGGCGGCCGGGGCGACCTCGCCCCGCTCGCCATGCTGGAGGACAGCGTCAAGCGCCTCAAGTCGCCCTCGGCGTCGCCGGGCGCGATGCTGCCGCGGCCGCAGGCCGAGGCCGCGCTCTCGCTGCTCGCCGACTGGTTCCTCGAGTCGTCCGGCAGCGCGTCCCTATCCGCGGTCGAGCACCCCAAGCTCAAGAACTTCCTGCGCCAGGTTGGGCTGCCGGAGATATCTCGGGCCGACCTCGCCGGCGCGCGCCTGGACACGCGCTTCGCCGAGGCTCGCGCCGACGCCGCCGCGCGCTTCCGCGAGGCGCGGTTCTTCCAGCTCGCGGCCGACGGGCTGCGCGAGCAGGTGATCACCCTCTCTGTCAACCTCCCCAACGACACGTCCGTGTTCCACCGCGCCGTGCCCATGCCCGCGCCGGCGTCGGCGTCCCCTGACTACGCCCAGGAGCTGTTCCTGGACGCGGCATCGTCCGTCTCCGCCTCCTCCGGCGACATTCGGCATTGCGCCGGCATCGTCGCCGACCGCTTCGGCTCCAAGACTCTTCGTGATCTCGAGACCAAGCACCATTGGATGGTGAACCTTACCTGCCAAGTCCATGGCCTGTCCCGCTTGATAAGCGACATGGCGCGCGAGCTCCCACTCTTCAACAACGCTGCCTCAAATTGCGCCAAGATCGCCAGCTACTTCAACACCACGCCCTCCGTGCGCGCGCTGCTGCACAAGCACCAGGTCCAGGAGCACGGTCACGCCTTCCTCCTCCCCATTGCCGCTCCGCCGTATAATGGCGGGGAATTTGCCGCCGCGTTCGTGATGCTCGAGAGCATCCTGACCTCGGCGCGGCCGCTCCAGCTCGCCGTGCTCGAGGAGTCCTACAAGGTGGTCTGCATCGACGACCCTGCCGCGAGGGAGATCGCGGCCATGGTGCAGAATGTGGCGTTCTGGACCGAGGTCGAGGCCACGCATTCGGTTGTGAAGCTGATCATGGACTTGGTGAAGGAGATGGAGACCGAGAGGCCCCTGGTCGGGCAATGCCTGCCGCTCTGGGAGGACCTGCGGGGCAAGGTCAGAGGCTGGTGCCGGAAATTCAGCGTAGAAGAGGCACCTGCCATGAATGTGGTCGAGAGTAGGTTCAGGAAGAACTACCACCCGGCGTGGTCAGCGGCATTCATACTGGACCCATTGTATCTGATCAAGGACGCCGGCAGGAGGTACCTTCCACCGTTCAACTACTTGACGCCAGAGCAGGAGAAGGACGTGGACAGGCTGATCACGAGGCTGGTGTCGCCGGAGGAGGCGCACCTCGCGCTGATGGAGCTGATGAAATGGCGGTCCGAGGGGCTCGACCCATTGTACGCGCAGGCGGTCCAGGTCCGGCAGCCAGACCCGTCGACGGGGAAGATGAAGATAGCCAACAAGCAGAGCAGCCGGCTTGTCTGGGAGACATGCCTCAGCGAGTTCAAGTCACTTGGCAAAGTGGCCGTCAGGCTCATCTTCCTCCATGCAACCGCCAAGGGGTTCAAATGCACGCCGTCGATGACCCGGTGGCTCACCGCGCCGGGCAGCTCGGCCGGCAGCATTGGGCGGGCGCACCGGCTGGTGTTCATCGCGGCGAATTCGAAGCTGGAGAGGAGGGATTTCTCAAACGACGACGACAAGGACGTGGAGCTGCTGACAGAAGGGGACGACGACATGCTAACCGAGACCGGCAATGTGGATCCCTCCTCATCATCAGTGTAGCATTCCATTCCATCCCTTCCTTGCTGCTGCTTAATTTCCTTGGCCCCTTTGTGCATTTGATGTTAGCATCCCTTAGAATTTGATCCCTCTAGGAATTGCTAGATTCTTTTTTTGCTTGTGCCCCCCATGTTGCTAGTTCTTGCTCCCCTCCTCTTGTTTTGGTTGGGTAGGGGGTAGGGGCATGAGAGATGGAGATATGCTGCACCCGGTCGATCGGTCTTAATTTGCTTGTCAAGTGTGGTTAATTAATAAACGGCTCATCCCTTGTCATCCCCTGCTGATTCTGCTGCTGGATCCTGAATGAAGTGTTTGGAAATCGAATTCAGAATCAGCAGGTGGCAAGATGAGTGAGCAGTGGGGGAGTGTGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTTAGCTATTGTGCAAATAATACTACTACTACCTAGTGCCGTTTATAC
CDS Sequence
  • >TraesCS2B02G073600.1
    ATGTCGGCGCCGGAGGAGGGCGACGCCGCCGCCTCAGGACCGTCGGCGCCGCCGCCGCCTCCCGCGCCCGCGCCGATGGCGATGGCGGAGCCCGGCGCAATGGGGCCCGAGGAGGCCGCCGCCAGGAAGCGCTACGAGGCGCTGATGCAGGTGCGGGCCAAGGCGCTCAAGGGCAAGGGCGCCTGGTACTGGGGCCACCTCGAGCCCGTCCTCGTCCCGCCGCCGGCCTCCGGGGCGCCCCCCAAGGCCGCCCGCCTCCGATGCGCGCTCTGCGCCGCCACCTTCTCCGCCTCCAACCCCTCCCGCACCGCCACCGAGCACCTCAAGCGCGGCGCCTGCCCTAATTTCGCGGCGGCGCAGGGCGCGCCGCCGCCGCCGCCGCCGCCGCCCCCGCCCCCGCCGCAGAATCAGCATCAGCAGTTGCAGCTCGTCGCCGTCTCGTCCCCCGCCTCATCGATCGTGCCCATCTCCTCCATCCCCCCGTCCTCCTCCTCCTCCTCGCAGCGCCGCCACTCCACCGGCGTTGGCGGCCGGAAGCGCCACGCGCTCGCCGCGGCCTACGCATCCGTCGAGGCGGCCGCGCACCAGCACCACGTCATGGTCGCCGACCCGGCCGGGTACTCCCCGACGCCGCCCACCCCGCCCGGGATGCCCGCGCCCAGGCAGCTGCTCTCCGGCGGCCGGGGCGACCTCGCCCCGCTCGCCATGCTGGAGGACAGCGTCAAGCGCCTCAAGTCGCCCTCGGCGTCGCCGGGCGCGATGCTGCCGCGGCCGCAGGCCGAGGCCGCGCTCTCGCTGCTCGCCGACTGGTTCCTCGAGTCGTCCGGCAGCGCGTCCCTATCCGCGGTCGAGCACCCCAAGCTCAAGAACTTCCTGCGCCAGGTTGGGCTGCCGGAGATATCTCGGGCCGACCTCGCCGGCGCGCGCCTGGACACGCGCTTCGCCGAGGCTCGCGCCGACGCCGCCGCGCGCTTCCGCGAGGCGCGGTTCTTCCAGCTCGCGGCCGACGGGCTGCGCGAGCAGGTGATCACCCTCTCTGTCAACCTCCCCAACGACACGTCCGTGTTCCACCGCGCCGTGCCCATGCCCGCGCCGGCGTCGGCGTCCCCTGACTACGCCCAGGAGCTGTTCCTGGACGCGGCATCGTCCGTCTCCGCCTCCTCCGGCGACATTCGGCATTGCGCCGGCATCGTCGCCGACCGCTTCGGCTCCAAGACTCTTCGTGATCTCGAGACCAAGCACCATTGGATGGTGAACCTTACCTGCCAAGTCCATGGCCTGTCCCGCTTGATAAGCGACATGGCGCGCGAGCTCCCACTCTTCAACAACGCTGCCTCAAATTGCGCCAAGATCGCCAGCTACTTCAACACCACGCCCTCCGTGCGCGCGCTGCTGCACAAGCACCAGGTCCAGGAGCACGGTCACGCCTTCCTCCTCCCCATTGCCGCTCCGCCGTATAATGGCGGGGAATTTGCCGCCGCGTTCGTGATGCTCGAGAGCATCCTGACCTCGGCGCGGCCGCTCCAGCTCGCCGTGCTCGAGGAGTCCTACAAGGTGGTCTGCATCGACGACCCTGCCGCGAGGGAGATCGCGGCCATGGTGCAGAATGTGGCGTTCTGGACCGAGGTCGAGGCCACGCATTCGGTTGTGAAGCTGATCATGGACTTGGTGAAGGAGATGGAGACCGAGAGGCCCCTGGTCGGGCAATGCCTGCCGCTCTGGGAGGACCTGCGGGGCAAGGTCAGAGGCTGGTGCCGGAAATTCAGCGTAGAAGAGGCACCTGCCATGAATGTGGTCGAGAGTAGGTTCAGGAAGAACTACCACCCGGCGTGGTCAGCGGCATTCATACTGGACCCATTGTATCTGATCAAGGACGCCGGCAGGAGGTACCTTCCACCGTTCAACTACTTGACGCCAGAGCAGGAGAAGGACGTGGACAGGCTGATCACGAGGCTGGTGTCGCCGGAGGAGGCGCACCTCGCGCTGATGGAGCTGATGAAATGGCGGTCCGAGGGGCTCGACCCATTGTACGCGCAGGCGGTCCAGGTCCGGCAGCCAGACCCGTCGACGGGGAAGATGAAGATAGCCAACAAGCAGAGCAGCCGGCTTGTCTGGGAGACATGCCTCAGCGAGTTCAAGTCACTTGGCAAAGTGGCCGTCAGGCTCATCTTCCTCCATGCAACCGCCAAGGGGTTCAAATGCACGCCGTCGATGACCCGGTGGCTCACCGCGCCGGGCAGCTCGGCCGGCAGCATTGGGCGGGCGCACCGGCTGGTGTTCATCGCGGCGAATTCGAAGCTGGAGAGGAGGGATTTCTCAAACGACGACGACAAGGACGTGGAGCTGCTGACAGAAGGGGACGACGACATGCTAACCGAGACCGGCAATGTGGATCCCTCCTCATCATCAGTGTAG
Protein Sequence
  • >TraesCS2B02G073600.1.cds1
    MSAPEEGDAAASGPSAPPPPPAPAPMAMAEPGAMGPEEAAARKRYEALMQVRAKALKGKGAWYWGHLEPVLVPPPASGAPPKAARLRCALCAATFSASNPSRTATEHLKRGACPNFAAAQGAPPPPPPPPPPPPQNQHQQLQLVAVSSPASSIVPISSIPPSSSSSSQRRHSTGVGGRKRHALAAAYASVEAAAHQHHVMVADPAGYSPTPPTPPGMPAPRQLLSGGRGDLAPLAMLEDSVKRLKSPSASPGAMLPRPQAEAALSLLADWFLESSGSASLSAVEHPKLKNFLRQVGLPEISRADLAGARLDTRFAEARADAAARFREARFFQLAADGLREQVITLSVNLPNDTSVFHRAVPMPAPASASPDYAQELFLDAASSVSASSGDIRHCAGIVADRFGSKTLRDLETKHHWMVNLTCQVHGLSRLISDMARELPLFNNAASNCAKIASYFNTTPSVRALLHKHQVQEHGHAFLLPIAAPPYNGGEFAAAFVMLESILTSARPLQLAVLEESYKVVCIDDPAAREIAAMVQNVAFWTEVEATHSVVKLIMDLVKEMETERPLVGQCLPLWEDLRGKVRGWCRKFSVEEAPAMNVVESRFRKNYHPAWSAAFILDPLYLIKDAGRRYLPPFNYLTPEQEKDVDRLITRLVSPEEAHLALMELMKWRSEGLDPLYAQAVQVRQPDPSTGKMKIANKQSSRLVWETCLSEFKSLGKVAVRLIFLHATAKGFKCTPSMTRWLTAPGSSAGSIGRAHRLVFIAANSKLERRDFSNDDDKDVELLTEGDDDMLTETGNVDPSSSSV